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The map label for this gene is nusA [H]

Identifier: 57237514

GI number: 57237514

Start: 462289

End: 463377

Strand: Direct

Name: nusA [H]

Synonym: CJE0510

Alternate gene names: 57237514

Gene position: 462289-463377 (Clockwise)

Preceding gene: 57237507

Following gene: 57237516

Centisome position: 26.0

GC content: 30.49

Gene sequence:

>1089_bases
ATGGAAAAGATAGCAGATATTATCGAATCTATTGCTAATGAAAAAAATTTAAACTTAGAGAATGTAAGAGAAAAGGTAGC
TACCGCTTTAATCAATACTGCAAAAAGAATTTATGGACAAGAATATGAGTTTTTTGTGGATCCTAAAAATTTAAATCTTT
ATCAAAAAATTACCATAGTAGCAGATAATGACGAGAGATTGCAAAATAAAAGTGAGAGTTTTATCGCGTTAAGTAAGGCA
AAATCAGAAGCTCCAGATGTTGAAATAGGAGATGAATTAACCTATGAATGTTCTTTGGAAAATTTAGGAAGAACTGCGGT
AAATACCTTGCATAAAGAACTTGAATATCATATACAAAAATTATTAGAACAAACTATTTTTGAAAAATATAAAAACAAAG
TAGGGCAAATGGTTTTTGGTACAGTTGTTAGAGTGGATAATGAAGAAAATACTTTTATTGAAATAGATGAATTAAGAGCT
TTTTTACCGAGAAAAAATCGCATTAAAGGGGAAAAATTTAAAATAGGTGATGTGGTAAAAGCTGTGATACGCCGTGTTTA
TACAGATAAAGGTATTAAAATCGAGCTTAGTCGTACAAGTCCTAAATTTTTAGAATGTTTACTTGAAGCTGAGGTTCCAG
AAATCAAAGATGGTTATGTCAATATTATAGGATGTGCTAGAATTCCAGGTGAAAGAGCAAAAATCATTCTTCAGGCCAAT
GGAGCAAATATCGATCCTGTAGGAGCTACAGTAGGTGTTAAAGGTGTAAGAATTAATGCGGTAAGCAAAGAGCTTCATAA
TGAAAATATTGATTGTATAGAATTTACAAATGAGAGTGAAATTTTAATTTCTCGTGCCTTGGCTCCTGCTATTGTAAATT
CTGTAAAAATTGAAGATAAAAAAGCTATTGTAAGTTTAAATAGCGAACAAAAAAGCAAAGCTATAGGAAAAAATGGTATT
AATATTCGCCTTGCTTCTATGTTAAGTGGTTATGAAATAGAGCTCAATGAATTAAGCTCATCGCAGCTAAATAATGCCAT
CAGCAATGAAGAAGCAATGAAAAATCTTCAAGATTTGTTTAAAATTTAA

Upstream 100 bases:

>100_bases
AATCTATAATTTTAGCAAATTTTACTTTTAATTAAACTTATTAAGGATATAATTTTTATCTACATTTTTAAATTTCCCAC
AAAAAAAGGTTTTATAAGAT

Downstream 100 bases:

>100_bases
AATATTTGTGAATTTTCACAAATATTTTTTATAAAACCACTTCCAATAAAATGCCGCAAAGATAAAAATCATACCCAATA
AACCTGTAATCAGTTTTAAA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MEKIADIIESIANEKNLNLENVREKVATALINTAKRIYGQEYEFFVDPKNLNLYQKITIVADNDERLQNKSESFIALSKA
KSEAPDVEIGDELTYECSLENLGRTAVNTLHKELEYHIQKLLEQTIFEKYKNKVGQMVFGTVVRVDNEENTFIEIDELRA
FLPRKNRIKGEKFKIGDVVKAVIRRVYTDKGIKIELSRTSPKFLECLLEAEVPEIKDGYVNIIGCARIPGERAKIILQAN
GANIDPVGATVGVKGVRINAVSKELHNENIDCIEFTNESEILISRALAPAIVNSVKIEDKKAIVSLNSEQKSKAIGKNGI
NIRLASMLSGYEIELNELSSSQLNNAISNEEAMKNLQDLFKI

Sequences:

>Translated_362_residues
MEKIADIIESIANEKNLNLENVREKVATALINTAKRIYGQEYEFFVDPKNLNLYQKITIVADNDERLQNKSESFIALSKA
KSEAPDVEIGDELTYECSLENLGRTAVNTLHKELEYHIQKLLEQTIFEKYKNKVGQMVFGTVVRVDNEENTFIEIDELRA
FLPRKNRIKGEKFKIGDVVKAVIRRVYTDKGIKIELSRTSPKFLECLLEAEVPEIKDGYVNIIGCARIPGERAKIILQAN
GANIDPVGATVGVKGVRINAVSKELHNENIDCIEFTNESEILISRALAPAIVNSVKIEDKKAIVSLNSEQKSKAIGKNGI
NIRLASMLSGYEIELNELSSSQLNNAISNEEAMKNLQDLFKI
>Mature_362_residues
MEKIADIIESIANEKNLNLENVREKVATALINTAKRIYGQEYEFFVDPKNLNLYQKITIVADNDERLQNKSESFIALSKA
KSEAPDVEIGDELTYECSLENLGRTAVNTLHKELEYHIQKLLEQTIFEKYKNKVGQMVFGTVVRVDNEENTFIEIDELRA
FLPRKNRIKGEKFKIGDVVKAVIRRVYTDKGIKIELSRTSPKFLECLLEAEVPEIKDGYVNIIGCARIPGERAKIILQAN
GANIDPVGATVGVKGVRINAVSKELHNENIDCIEFTNESEILISRALAPAIVNSVKIEDKKAIVSLNSEQKSKAIGKNGI
NIRLASMLSGYEIELNELSSSQLNNAISNEEAMKNLQDLFKI

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=335, Percent_Identity=31.044776119403, Blast_Score=154, Evalue=8e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N [H]

EC number: NA

Molecular weight: Translated: 40642; Mature: 40642

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIADIIESIANEKNLNLENVREKVATALINTAKRIYGQEYEFFVDPKNLNLYQKITIV
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEE
ADNDERLQNKSESFIALSKAKSEAPDVEIGDELTYECSLENLGRTAVNTLHKELEYHIQK
ECCHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEECHHHCCHHHHHHHHHHHHHHHHH
LLEQTIFEKYKNKVGQMVFGTVVRVDNEENTFIEIDELRAFLPRKNRIKGEKFKIGDVVK
HHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEHHHHHHHCCCCCCCCCCCEEHHHHHH
AVIRRVYTDKGIKIELSRTSPKFLECLLEAEVPEIKDGYVNIIGCARIPGERAKIILQAN
HHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEEEEEEC
GANIDPVGATVGVKGVRINAVSKELHNENIDCIEFTNESEILISRALAPAIVNSVKIEDK
CCCCCCCCCEECCCCEEEEHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCEECCC
KAIVSLNSEQKSKAIGKNGINIRLASMLSGYEIELNELSSSQLNNAISNEEAMKNLQDLF
CEEEEECCHHHHHHHCCCCCEEEEEHHHCCCEEEHHHCCHHHHHHHHCHHHHHHHHHHHH
KI
CC
>Mature Secondary Structure
MEKIADIIESIANEKNLNLENVREKVATALINTAKRIYGQEYEFFVDPKNLNLYQKITIV
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEE
ADNDERLQNKSESFIALSKAKSEAPDVEIGDELTYECSLENLGRTAVNTLHKELEYHIQK
ECCHHHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEECHHHCCHHHHHHHHHHHHHHHHH
LLEQTIFEKYKNKVGQMVFGTVVRVDNEENTFIEIDELRAFLPRKNRIKGEKFKIGDVVK
HHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEHHHHHHHCCCCCCCCCCCEEHHHHHH
AVIRRVYTDKGIKIELSRTSPKFLECLLEAEVPEIKDGYVNIIGCARIPGERAKIILQAN
HHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEEEEEEC
GANIDPVGATVGVKGVRINAVSKELHNENIDCIEFTNESEILISRALAPAIVNSVKIEDK
CCCCCCCCCEECCCCEEEEHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCEECCC
KAIVSLNSEQKSKAIGKNGINIRLASMLSGYEIELNELSSSQLNNAISNEEAMKNLQDLF
CEEEEECCHHHHHHHCCCCCEEEEEHHHCCCEEEHHHCCHHHHHHHHCHHHHHHHHHHHH
KI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]