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The map label for this gene is thiC [H]

Identifier: 57237507

GI number: 57237507

Start: 456731

End: 458023

Strand: Direct

Name: thiC [H]

Synonym: CJE0503

Alternate gene names: 57237507

Gene position: 456731-458023 (Clockwise)

Preceding gene: 57237506

Following gene: 57237514

Centisome position: 25.69

GC content: 35.65

Gene sequence:

>1293_bases
ATGAAAACTCAAATGAATTATGCCAAAGAAGGTATTTTTACTAAAGAAATGCAGATTGTTGCACAAAAAGAAAATTTAAG
TAAAGATTTTTTGCTTGAAAATATTGCTTGCGGTAAAATTATTATCCCTGCAAATATTAATCATAAAAGTCTTGATCCAA
ATGGTATAGGTTTTGGACTGCGTACTAAGGTTAATGTAAATTTAGGAGTTTCAAATGATTGCGTTGATTATAGCGAGGAA
ATGAAAAAAGTAGAGCTTGCTCATAAATTTGACATAGAAGCGATTATGGACTTAAGCAATTATGGCAAAACAAGTCGTTT
TAGAGACGAGCTTGTTAATGTTTCAAAAGCTATGATAGGAACAGTTCCTGTATATGATGCGGTAGGATTTTTAGAAAAAG
ATTTAAAACAAATTGGTGCTAAAGACTTTTTAGATGTTGTATATCATCATGCTAAAAGTGGGGTTGATTTTATGACAATT
CATGCAGGTATTAATTCTCGCGCAGCGCATATTTTTAAACAAAGTAAAAGACTTACAAATATAGTTTCAAGAGGAGGCTC
TGTGCTTTATGCTTGGATGATGATGAAAGATGCTGAGAATCCTTTTTTTGAGTATTATGATGATTTGCTTGACATTTGTT
TAAAATACGATGTAACTTTATCTTTGGGCGATGCTTTGCGTCCTGGCTCTACTCACGATGCAAGTGATGGGGCGCAAATT
TCAGAGCTTATAGAATTGTCACTCCTTACTCAAAGAGCTTGGGATGTTGGGGTTCAAGTAATGATAGAAGGTCCAGGGCA
TATGGCTATTAATGAAATAGAGGCAAATATGCAATTAGAAAAGCGTTTATGCAAAGGAGCGCCTTTTTATGTCTTAGGAC
CTTTGGTAACAGATATTGGCGCAGGGTATGATCATATTAGTGGTGCTATTGGAGGAGCTGTGGCTGCAGCTAGCGGTGCT
GATATGCTGTGTTATGTAACACCTGCTGAACACTTAAGACTTCCAAATTTAGAAGATGTTAGAGAGGGTATAGTTGCGAC
TAAAATTGCAGCTCATGCGGGAGATATAGCTAAACTTCCAAAAGAAAGAGCAAGAGATGATGAGATGAGTAAGGCTAGAC
AAGAGATAGATTGGGAGAAAATGTTTAAACTTGCTATTGATGGAGAAAAGGCTAAAAAAATGTTTAATGAGCGTCGCCCT
GATGATTTAAACTCATGTTCCATGTGTGGAAAAATGTGTGCAATGAATACTATGAATCAAATTTTAAAAGGCGAAGATGT
GAGTTTAGCTTGA

Upstream 100 bases:

>100_bases
GGATCTGGATAATACCAGCGTCGGGAAGATTTATAAATTTCAATACTTTAAAAATTTATACTCACCCTTCATTTATCCAA
ATTTTTTAAAGGATAAATTT

Downstream 100 bases:

>100_bases
ATAAACTTTGAGAGTAAGATCTATAGAGATACCTTGCTCTCCTGCTTTAAGATTAAGTGGATAATCAAGCTTGATTAAGT
TATTATAGTTTTTTAGAGCA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKTQMNYAKEGIFTKEMQIVAQKENLSKDFLLENIACGKIIIPANINHKSLDPNGIGFGLRTKVNVNLGVSNDCVDYSEE
MKKVELAHKFDIEAIMDLSNYGKTSRFRDELVNVSKAMIGTVPVYDAVGFLEKDLKQIGAKDFLDVVYHHAKSGVDFMTI
HAGINSRAAHIFKQSKRLTNIVSRGGSVLYAWMMMKDAENPFFEYYDDLLDICLKYDVTLSLGDALRPGSTHDASDGAQI
SELIELSLLTQRAWDVGVQVMIEGPGHMAINEIEANMQLEKRLCKGAPFYVLGPLVTDIGAGYDHISGAIGGAVAAASGA
DMLCYVTPAEHLRLPNLEDVREGIVATKIAAHAGDIAKLPKERARDDEMSKARQEIDWEKMFKLAIDGEKAKKMFNERRP
DDLNSCSMCGKMCAMNTMNQILKGEDVSLA

Sequences:

>Translated_430_residues
MKTQMNYAKEGIFTKEMQIVAQKENLSKDFLLENIACGKIIIPANINHKSLDPNGIGFGLRTKVNVNLGVSNDCVDYSEE
MKKVELAHKFDIEAIMDLSNYGKTSRFRDELVNVSKAMIGTVPVYDAVGFLEKDLKQIGAKDFLDVVYHHAKSGVDFMTI
HAGINSRAAHIFKQSKRLTNIVSRGGSVLYAWMMMKDAENPFFEYYDDLLDICLKYDVTLSLGDALRPGSTHDASDGAQI
SELIELSLLTQRAWDVGVQVMIEGPGHMAINEIEANMQLEKRLCKGAPFYVLGPLVTDIGAGYDHISGAIGGAVAAASGA
DMLCYVTPAEHLRLPNLEDVREGIVATKIAAHAGDIAKLPKERARDDEMSKARQEIDWEKMFKLAIDGEKAKKMFNERRP
DDLNSCSMCGKMCAMNTMNQILKGEDVSLA
>Mature_430_residues
MKTQMNYAKEGIFTKEMQIVAQKENLSKDFLLENIACGKIIIPANINHKSLDPNGIGFGLRTKVNVNLGVSNDCVDYSEE
MKKVELAHKFDIEAIMDLSNYGKTSRFRDELVNVSKAMIGTVPVYDAVGFLEKDLKQIGAKDFLDVVYHHAKSGVDFMTI
HAGINSRAAHIFKQSKRLTNIVSRGGSVLYAWMMMKDAENPFFEYYDDLLDICLKYDVTLSLGDALRPGSTHDASDGAQI
SELIELSLLTQRAWDVGVQVMIEGPGHMAINEIEANMQLEKRLCKGAPFYVLGPLVTDIGAGYDHISGAIGGAVAAASGA
DMLCYVTPAEHLRLPNLEDVREGIVATKIAAHAGDIAKLPKERARDDEMSKARQEIDWEKMFKLAIDGEKAKKMFNERRP
DDLNSCSMCGKMCAMNTMNQILKGEDVSLA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=452, Percent_Identity=41.3716814159292, Blast_Score=351, Evalue=7e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 47512; Mature: 47512

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQMNYAKEGIFTKEMQIVAQKENLSKDFLLENIACGKIIIPANINHKSLDPNGIGFGL
CCCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCE
RTKVNVNLGVSNDCVDYSEEMKKVELAHKFDIEAIMDLSNYGKTSRFRDELVNVSKAMIG
EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
TVPVYDAVGFLEKDLKQIGAKDFLDVVYHHAKSGVDFMTIHAGINSRAAHIFKQSKRLTN
CCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHH
IVSRGGSVLYAWMMMKDAENPFFEYYDDLLDICLKYDVTLSLGDALRPGSTHDASDGAQI
HHHCCCHHEEHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHH
SELIELSLLTQRAWDVGVQVMIEGPGHMAINEIEANMQLEKRLCKGAPFYVLGPLVTDIG
HHHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHHHHHHHCCCCCEEEHHHHHHHC
AGYDHISGAIGGAVAAASGADMLCYVTPAEHLRLPNLEDVREGIVATKIAAHAGDIAKLP
CCHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCC
KERARDDEMSKARQEIDWEKMFKLAIDGEKAKKMFNERRPDDLNSCSMCGKMCAMNTMNQ
HHHCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ILKGEDVSLA
HHCCCCCCCC
>Mature Secondary Structure
MKTQMNYAKEGIFTKEMQIVAQKENLSKDFLLENIACGKIIIPANINHKSLDPNGIGFGL
CCCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCE
RTKVNVNLGVSNDCVDYSEEMKKVELAHKFDIEAIMDLSNYGKTSRFRDELVNVSKAMIG
EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
TVPVYDAVGFLEKDLKQIGAKDFLDVVYHHAKSGVDFMTIHAGINSRAAHIFKQSKRLTN
CCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHH
IVSRGGSVLYAWMMMKDAENPFFEYYDDLLDICLKYDVTLSLGDALRPGSTHDASDGAQI
HHHCCCHHEEHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHH
SELIELSLLTQRAWDVGVQVMIEGPGHMAINEIEANMQLEKRLCKGAPFYVLGPLVTDIG
HHHHHHHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHHHHHHHCCCCCEEEHHHHHHHC
AGYDHISGAIGGAVAAASGADMLCYVTPAEHLRLPNLEDVREGIVATKIAAHAGDIAKLP
CCHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHCC
KERARDDEMSKARQEIDWEKMFKLAIDGEKAKKMFNERRPDDLNSCSMCGKMCAMNTMNQ
HHHCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ILKGEDVSLA
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA