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The map label for this gene is glyA [H]

Identifier: 57237457

GI number: 57237457

Start: 404904

End: 406148

Strand: Direct

Name: glyA [H]

Synonym: CJE0451

Alternate gene names: 57237457

Gene position: 404904-406148 (Clockwise)

Preceding gene: 57237456

Following gene: 57237458

Centisome position: 22.78

GC content: 33.17

Gene sequence:

>1245_bases
ATGAGTTTAGAAATGTTTGATAAAGAAATTTTTGATTTAACAAACAAAGAGTTAGAGCGTCAATGCGAAGGTCTTGAAAT
GATAGCGAGTGAAAATTTCACTTTACCTGAAGTAATGGAAGTTATGGGAAGTATCTTGACGAACAAATACGCAGAAGGTT
ATCCAGGTAAAAGATATTATGGTGGTTGTGAATTTGTTGATGAGATTGAAACTCTAGCTATTGAAAGATGTAAAAAACTT
TTTAATTGTAAATTTGCTAATGTTCAGCCTAATTCAGGTTCTCAAGCTAATCAAGGTGTTTATGCGGCTTTGATTAATCC
AGGTGATAAAATTTTAGGAATGGATTTAAGTCATGGTGGACATTTAACTCATGGTGCAAAAGTAAGTTCTTCGGGTAAAA
TGTACGAAAGTTGTTTTTACGGCGTAGAACTTGATGGAAGAATTGATTATGAAAAAGTAAGAGAAATTGCTAAAAAAGAA
AAGCCAAAATTAATAGTTTGTGGAGCTAGTGCTTATGCAAGAGTGATTGATTTTGCTAAATTTAGAGAAATCGCTGATGA
AGTAGGCGCCTATCTTTTTGCTGATATAGCACATATTGCAGGTCTTGTTGTAGCAGGCGAACATCCAAGTCCTTTTCCAC
ACGCTCATGTAGTAAGTTCAACTACGCATAAAACTTTGCGTGGCCCAAGAGGTGGTATTATTATGACAAATGATGAAGAG
CTTGCTAAAAAAATTAATTCTGCCATTTTTCCAGGTATTCAAGGTGGTCCTTTGATGCATGTAATTGCTGCAAAAGCAGT
AGGATTTAAATTTAATCTTAGCGATGAGTGGAAAGTTTATGCAAAACAAGTAAGAACCAATGCTCAAGTTTTAGCTAATG
TTTTAATGGATAGAAAATTTAAACTTGTTAGCGATGGAACGGATAATCACCTTGTTTTAATGAGTTTTTTAGATCGTGAG
TTTAGCGGAAAAGATGCAGATTTAGCTCTTGGAAATGCAGGTATTACTGCAAATAAAAATACCGTTCCAGGAGAGATTAG
AAGTCCTTTTATCACAAGTGGATTAAGACTTGGAACTCCAGCCCTTACTGCCAGAGGTTTTAAAGAAAAAGAAATGGAAA
TTGTGTCAAATTATATTGCAGATATTTTAGATGATGTTAATAATGAAAAATTACAAAAGAATATTAAACAAGAATTAAAA
AAACTTGCAAGTAATTTTATTATTTATGAAAGGGCTATGTTTTGA

Upstream 100 bases:

>100_bases
TAGATAGACTTGTTATGCTTTTGACAAATAAAAAATCTATTCGAGATGTGATATTGTTTCCTGCAATGCGTCCATTAAAA
TCAGAACTAAAGGAGAAAGA

Downstream 100 bases:

>100_bases
TTTCACCAATGGATATGTCGCTAATTAAAATAATTGGCGATCATTATTATATAAGACGCGATAAAATTGTAAATAAAATT
ACACATAGAGGTCGCTTATT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSLEMFDKEIFDLTNKELERQCEGLEMIASENFTLPEVMEVMGSILTNKYAEGYPGKRYYGGCEFVDEIETLAIERCKKL
FNCKFANVQPNSGSQANQGVYAALINPGDKILGMDLSHGGHLTHGAKVSSSGKMYESCFYGVELDGRIDYEKVREIAKKE
KPKLIVCGASAYARVIDFAKFREIADEVGAYLFADIAHIAGLVVAGEHPSPFPHAHVVSSTTHKTLRGPRGGIIMTNDEE
LAKKINSAIFPGIQGGPLMHVIAAKAVGFKFNLSDEWKVYAKQVRTNAQVLANVLMDRKFKLVSDGTDNHLVLMSFLDRE
FSGKDADLALGNAGITANKNTVPGEIRSPFITSGLRLGTPALTARGFKEKEMEIVSNYIADILDDVNNEKLQKNIKQELK
KLASNFIIYERAMF

Sequences:

>Translated_414_residues
MSLEMFDKEIFDLTNKELERQCEGLEMIASENFTLPEVMEVMGSILTNKYAEGYPGKRYYGGCEFVDEIETLAIERCKKL
FNCKFANVQPNSGSQANQGVYAALINPGDKILGMDLSHGGHLTHGAKVSSSGKMYESCFYGVELDGRIDYEKVREIAKKE
KPKLIVCGASAYARVIDFAKFREIADEVGAYLFADIAHIAGLVVAGEHPSPFPHAHVVSSTTHKTLRGPRGGIIMTNDEE
LAKKINSAIFPGIQGGPLMHVIAAKAVGFKFNLSDEWKVYAKQVRTNAQVLANVLMDRKFKLVSDGTDNHLVLMSFLDRE
FSGKDADLALGNAGITANKNTVPGEIRSPFITSGLRLGTPALTARGFKEKEMEIVSNYIADILDDVNNEKLQKNIKQELK
KLASNFIIYERAMF
>Mature_413_residues
SLEMFDKEIFDLTNKELERQCEGLEMIASENFTLPEVMEVMGSILTNKYAEGYPGKRYYGGCEFVDEIETLAIERCKKLF
NCKFANVQPNSGSQANQGVYAALINPGDKILGMDLSHGGHLTHGAKVSSSGKMYESCFYGVELDGRIDYEKVREIAKKEK
PKLIVCGASAYARVIDFAKFREIADEVGAYLFADIAHIAGLVVAGEHPSPFPHAHVVSSTTHKTLRGPRGGIIMTNDEEL
AKKINSAIFPGIQGGPLMHVIAAKAVGFKFNLSDEWKVYAKQVRTNAQVLANVLMDRKFKLVSDGTDNHLVLMSFLDREF
SGKDADLALGNAGITANKNTVPGEIRSPFITSGLRLGTPALTARGFKEKEMEIVSNYIADILDDVNNEKLQKNIKQELKK
LASNFIIYERAMF

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=450, Percent_Identity=43.7777777777778, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI261862350, Length=450, Percent_Identity=43.7777777777778, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI261862348, Length=450, Percent_Identity=43.7777777777778, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI19923315, Length=450, Percent_Identity=44, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI261862346, Length=449, Percent_Identity=43.4298440979955, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI22547186, Length=397, Percent_Identity=48.1108312342569, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI22547189, Length=383, Percent_Identity=45.9530026109661, Blast_Score=300, Evalue=2e-81,
Organism=Escherichia coli, GI1788902, Length=411, Percent_Identity=55.4744525547445, Blast_Score=470, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI25144732, Length=400, Percent_Identity=46.5, Blast_Score=344, Evalue=5e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=400, Percent_Identity=46.5, Blast_Score=343, Evalue=7e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=414, Percent_Identity=43.9613526570048, Blast_Score=338, Evalue=9e-94,
Organism=Saccharomyces cerevisiae, GI6323087, Length=421, Percent_Identity=40.6175771971496, Blast_Score=306, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=48.75, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=48.75, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45737; Mature: 45606

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEMFDKEIFDLTNKELERQCEGLEMIASENFTLPEVMEVMGSILTNKYAEGYPGKRYY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
GGCEFVDEIETLAIERCKKLFNCKFANVQPNSGSQANQGVYAALINPGDKILGMDLSHGG
CCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCC
HLTHGAKVSSSGKMYESCFYGVELDGRIDYEKVREIAKKEKPKLIVCGASAYARVIDFAK
CCCCCCEECCCCCHHHHHEECEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHH
FREIADEVGAYLFADIAHIAGLVVAGEHPSPFPHAHVVSSTTHKTLRGPRGGIIMTNDEE
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHCCCCHHHHHCCCCCCEEEECCHH
LAKKINSAIFPGIQGGPLMHVIAAKAVGFKFNLSDEWKVYAKQVRTNAQVLANVLMDRKF
HHHHHHHHHCCCCCCCHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCE
KLVSDGTDNHLVLMSFLDREFSGKDADLALGNAGITANKNTVPGEIRSPFITSGLRLGTP
EEECCCCCCCEEEHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHCCCHHHCCCCCCCC
ALTARGFKEKEMEIVSNYIADILDDVNNEKLQKNIKQELKKLASNFIIYERAMF
HHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLEMFDKEIFDLTNKELERQCEGLEMIASENFTLPEVMEVMGSILTNKYAEGYPGKRYY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
GGCEFVDEIETLAIERCKKLFNCKFANVQPNSGSQANQGVYAALINPGDKILGMDLSHGG
CCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCC
HLTHGAKVSSSGKMYESCFYGVELDGRIDYEKVREIAKKEKPKLIVCGASAYARVIDFAK
CCCCCCEECCCCCHHHHHEECEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHH
FREIADEVGAYLFADIAHIAGLVVAGEHPSPFPHAHVVSSTTHKTLRGPRGGIIMTNDEE
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHCCCCHHHHHCCCCCCEEEECCHH
LAKKINSAIFPGIQGGPLMHVIAAKAVGFKFNLSDEWKVYAKQVRTNAQVLANVLMDRKF
HHHHHHHHHCCCCCCCHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCE
KLVSDGTDNHLVLMSFLDREFSGKDADLALGNAGITANKNTVPGEIRSPFITSGLRLGTP
EEECCCCCCCEEEHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHCCCHHHCCCCCCCC
ALTARGFKEKEMEIVSNYIADILDDVNNEKLQKNIKQELKKLASNFIIYERAMF
HHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA