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The map label for this gene is lysS [H]

Identifier: 57237456

GI number: 57237456

Start: 403402

End: 404907

Strand: Direct

Name: lysS [H]

Synonym: CJE0450

Alternate gene names: 57237456

Gene position: 403402-404907 (Clockwise)

Preceding gene: 57237455

Following gene: 57237457

Centisome position: 22.69

GC content: 31.54

Gene sequence:

>1506_bases
ATGTTTGATAATATTTTAGAGCAGCAAAGAATAGAAAAAGCTAAAGAGTTAAAAAATTTAGGGATTAATCCTTATCCTCA
TTTTTTAGAAAAGGAAATGTCTTTAAAGACTTTTAAGGATAGATTTTCTTATATTTTAGAGCAAGTTGAAAAAAGAGATG
AAAGTGTCAATGCTGTTGTTGCAGGACGTTTGAAACTTTTGCGTATAGCTGGGAAATCAATTTTTGCAAACATTGAAGAT
GAGGATACAAATTTACAAATTTATTTTAGTAAAGATAGCGTAGGCGAAGAGCTTTATACGATTTTAAAAAAGAATTTAGA
GGTGGGTGATATTGTTTTGGTTAAAGGTTTTCCTTTTGTAACTAAAACAGGTGAGTTTAGTTTGCATGCAAGTGAAGTAA
AACTCGCTACAAAAGCTATTGTTCCTTTACCTGAAAAATATCATGGATTAACAGATATTGAACAAAGATATCGTAAACGT
TATGTTGATATGATAATGAATGCAGAAGTTAGAAAAGATTTTTTAGTGCGTTCTAAAGTGGTGAGTTTGATTCGTCATTT
TTTTGAAAATAAAGGCTTTTTAGAAGTAGAAACTCCTATGATGCATCCGATTGCAGGTGGAGCAAACGCTAAGCCTTTTG
TAACTTTTCACAATTCTTTAGGCGTAGAAAGATTTTTAAGAATTGCTCCAGAACTTTATCTTAAAAGACTTGTAGTGGGT
GGTTTTGAGGCAGTTTTTGAAATCAATCGTTGTTTTAGAAACGAAGGTATGGATTTAACTCATAATCCCGAATTTACAAC
TATAGAATTTTATTGGGCTTATCATAATTATAAAGATTTAATGGATTTAACTGAAGAACTTTTTGCTTTGCTTTTAGATA
AACTTAATTTGGGAAAAACAATAGAATTTGATGGAAAAATGATAGATTTTTCTAAACCTTTTGAAAGAATAACCTATAAA
GATGCACTTTGTAAGTATGGTGGTTTAGATAGGGATTTAATCGAAGATAAGGAAAAAATTCTTACTAAATTAAAAGCAGA
TGGTTTTGAAGCGAATGAAAAGCTTGAACTTGGACATTTGCAAGCTGAACTTTTTGACAATTATGTGGAAGAAAAATTGA
TCAATCCTACTTTTGTGATTGATTTTCCTATTTCCATAAGTCCTTTATCAAGACGTAGCGATGAGGATTCTCAGATTGCT
GAGAGATTTGAGTTGTTTATTTGTGGAAGAGAATTGGCAAATGGTTTTAATGAGCTTAATGATCCTCTTGATCAATATGA
GAGATTTTTAAAACAAATTGAAGCTAAAAATGCAGGCGATGAAGAGGCTTGCGAAATGGATGAAGATTTTGTAAATGCTT
TGGGTTATGGAATGCCTCCAACGGCAGGGCAGGGTATAGGCATAGATAGACTTGTTATGCTTTTGACAAATAAAAAATCT
ATTCGAGATGTGATATTGTTTCCTGCAATGCGTCCATTAAAATCAGAACTAAAGGAGAAAGAATGA

Upstream 100 bases:

>100_bases
GATTGCAAAAGAACATGGTTTTAAACTTACAGGGCATTTGATGCAGCTTTATGGTGTTTGTGGTGATTGTAATAATCAAA
AAGTAAAGGTAAAAATATAA

Downstream 100 bases:

>100_bases
GTTTAGAAATGTTTGATAAAGAAATTTTTGATTTAACAAACAAAGAGTTAGAGCGTCAATGCGAAGGTCTTGAAATGATA
GCGAGTGAAAATTTCACTTT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MFDNILEQQRIEKAKELKNLGINPYPHFLEKEMSLKTFKDRFSYILEQVEKRDESVNAVVAGRLKLLRIAGKSIFANIED
EDTNLQIYFSKDSVGEELYTILKKNLEVGDIVLVKGFPFVTKTGEFSLHASEVKLATKAIVPLPEKYHGLTDIEQRYRKR
YVDMIMNAEVRKDFLVRSKVVSLIRHFFENKGFLEVETPMMHPIAGGANAKPFVTFHNSLGVERFLRIAPELYLKRLVVG
GFEAVFEINRCFRNEGMDLTHNPEFTTIEFYWAYHNYKDLMDLTEELFALLLDKLNLGKTIEFDGKMIDFSKPFERITYK
DALCKYGGLDRDLIEDKEKILTKLKADGFEANEKLELGHLQAELFDNYVEEKLINPTFVIDFPISISPLSRRSDEDSQIA
ERFELFICGRELANGFNELNDPLDQYERFLKQIEAKNAGDEEACEMDEDFVNALGYGMPPTAGQGIGIDRLVMLLTNKKS
IRDVILFPAMRPLKSELKEKE

Sequences:

>Translated_501_residues
MFDNILEQQRIEKAKELKNLGINPYPHFLEKEMSLKTFKDRFSYILEQVEKRDESVNAVVAGRLKLLRIAGKSIFANIED
EDTNLQIYFSKDSVGEELYTILKKNLEVGDIVLVKGFPFVTKTGEFSLHASEVKLATKAIVPLPEKYHGLTDIEQRYRKR
YVDMIMNAEVRKDFLVRSKVVSLIRHFFENKGFLEVETPMMHPIAGGANAKPFVTFHNSLGVERFLRIAPELYLKRLVVG
GFEAVFEINRCFRNEGMDLTHNPEFTTIEFYWAYHNYKDLMDLTEELFALLLDKLNLGKTIEFDGKMIDFSKPFERITYK
DALCKYGGLDRDLIEDKEKILTKLKADGFEANEKLELGHLQAELFDNYVEEKLINPTFVIDFPISISPLSRRSDEDSQIA
ERFELFICGRELANGFNELNDPLDQYERFLKQIEAKNAGDEEACEMDEDFVNALGYGMPPTAGQGIGIDRLVMLLTNKKS
IRDVILFPAMRPLKSELKEKE
>Mature_501_residues
MFDNILEQQRIEKAKELKNLGINPYPHFLEKEMSLKTFKDRFSYILEQVEKRDESVNAVVAGRLKLLRIAGKSIFANIED
EDTNLQIYFSKDSVGEELYTILKKNLEVGDIVLVKGFPFVTKTGEFSLHASEVKLATKAIVPLPEKYHGLTDIEQRYRKR
YVDMIMNAEVRKDFLVRSKVVSLIRHFFENKGFLEVETPMMHPIAGGANAKPFVTFHNSLGVERFLRIAPELYLKRLVVG
GFEAVFEINRCFRNEGMDLTHNPEFTTIEFYWAYHNYKDLMDLTEELFALLLDKLNLGKTIEFDGKMIDFSKPFERITYK
DALCKYGGLDRDLIEDKEKILTKLKADGFEANEKLELGHLQAELFDNYVEEKLINPTFVIDFPISISPLSRRSDEDSQIA
ERFELFICGRELANGFNELNDPLDQYERFLKQIEAKNAGDEEACEMDEDFVNALGYGMPPTAGQGIGIDRLVMLLTNKKS
IRDVILFPAMRPLKSELKEKE

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=498, Percent_Identity=42.570281124498, Blast_Score=381, Evalue=1e-105,
Organism=Homo sapiens, GI194272210, Length=498, Percent_Identity=42.570281124498, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI45439306, Length=476, Percent_Identity=24.5798319327731, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1789256, Length=492, Percent_Identity=48.9837398373984, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1790571, Length=492, Percent_Identity=48.1707317073171, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI87082379, Length=329, Percent_Identity=34.3465045592705, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788173, Length=299, Percent_Identity=24.7491638795987, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=499, Percent_Identity=42.4849699398798, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535925, Length=499, Percent_Identity=42.4849699398798, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71994340, Length=499, Percent_Identity=42.4849699398798, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17551876, Length=353, Percent_Identity=24.3626062322946, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=209, Percent_Identity=28.2296650717703, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=511, Percent_Identity=40.5088062622309, Blast_Score=354, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6324256, Length=505, Percent_Identity=31.2871287128713, Blast_Score=200, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6323011, Length=368, Percent_Identity=26.6304347826087, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321807, Length=322, Percent_Identity=23.2919254658385, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=170, Percent_Identity=30, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640851, Length=499, Percent_Identity=42.4849699398798, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640849, Length=499, Percent_Identity=42.4849699398798, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17136276, Length=456, Percent_Identity=21.7105263157895, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24584738, Length=305, Percent_Identity=27.2131147540984, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57833; Mature: 57833

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDNILEQQRIEKAKELKNLGINPYPHFLEKEMSLKTFKDRFSYILEQVEKRDESVNAVV
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRLKLLRIAGKSIFANIEDEDTNLQIYFSKDSVGEELYTILKKNLEVGDIVLVKGFPFV
HHHHHHHHHHCHHHEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCE
TKTGEFSLHASEVKLATKAIVPLPEKYHGLTDIEQRYRKRYVDMIMNAEVRKDFLVRSKV
EECCCCEEEHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSLIRHFFENKGFLEVETPMMHPIAGGANAKPFVTFHNSLGVERFLRIAPELYLKRLVVG
HHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GFEAVFEINRCFRNEGMDLTHNPEFTTIEFYWAYHNYKDLMDLTEELFALLLDKLNLGKT
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCE
IEFDGKMIDFSKPFERITYKDALCKYGGLDRDLIEDKEKILTKLKADGFEANEKLELGHL
EECCCCEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
QAELFDNYVEEKLINPTFVIDFPISISPLSRRSDEDSQIAERFELFICGRELANGFNELN
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHEECHHHHHHHHHHC
DPLDQYERFLKQIEAKNAGDEEACEMDEDFVNALGYGMPPTAGQGIGIDRLVMLLTNKKS
CHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHH
IRDVILFPAMRPLKSELKEKE
HHHHHHHHCHHHHHHHHCCCC
>Mature Secondary Structure
MFDNILEQQRIEKAKELKNLGINPYPHFLEKEMSLKTFKDRFSYILEQVEKRDESVNAVV
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRLKLLRIAGKSIFANIEDEDTNLQIYFSKDSVGEELYTILKKNLEVGDIVLVKGFPFV
HHHHHHHHHHCHHHEECCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCE
TKTGEFSLHASEVKLATKAIVPLPEKYHGLTDIEQRYRKRYVDMIMNAEVRKDFLVRSKV
EECCCCEEEHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSLIRHFFENKGFLEVETPMMHPIAGGANAKPFVTFHNSLGVERFLRIAPELYLKRLVVG
HHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
GFEAVFEINRCFRNEGMDLTHNPEFTTIEFYWAYHNYKDLMDLTEELFALLLDKLNLGKT
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCE
IEFDGKMIDFSKPFERITYKDALCKYGGLDRDLIEDKEKILTKLKADGFEANEKLELGHL
EECCCCEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
QAELFDNYVEEKLINPTFVIDFPISISPLSRRSDEDSQIAERFELFICGRELANGFNELN
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHEECHHHHHHHHHHC
DPLDQYERFLKQIEAKNAGDEEACEMDEDFVNALGYGMPPTAGQGIGIDRLVMLLTNKKS
CHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHH
IRDVILFPAMRPLKSELKEKE
HHHHHHHHCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA