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The map label for this gene is trpS

Identifier: 57237443

GI number: 57237443

Start: 390995

End: 391954

Strand: Direct

Name: trpS

Synonym: CJE0437

Alternate gene names: 57237443

Gene position: 390995-391954 (Clockwise)

Preceding gene: 57237442

Following gene: 57237444

Centisome position: 21.99

GC content: 33.12

Gene sequence:

>960_bases
ATGAGAGTATTAACAGGCCTTCAGCCAAGTGGCGATTTGCATATAGGTAATTATTTTGGTGCGATTAAGCAAATGGTAGA
TGCACAAGAAAAAAGCCAAATGTTTATGTTTATTGCTAATTATCATGCCATGACTTCTTCTCAAGATGGAGAAAAACTTA
AACAAAATTCCTTAAAAGCTGCTGCAGCTTTTTTAAGCCTTGGTATAGATCCTCAAAAAAGCGTTTTTTGGTTGCAAAGT
GATGTTAAGGAAGTTATGGAGCTTTATTGGATTTTATCGCAATTTACTCCTATGGGTTTATTAGAGCGTGCACATAGTTA
CAAGGATAAAGTTGCTAAGGGACTTAGTGCTTCACATGGGCTTTTTTCTTATCCTGTTTTAATGGCGGCAGATATTTTAC
TTTTTGATACACGAATTGTCCCTGTAGGAAAAGATCAAATCCAGCATGTTGAAATCGCACGCGATATTGCTTTAAAAGTT
AATAATGAATGGGGTGAAATTTTTACCCTTCCAGAAGCGAGAGTTAATGAAGAAGTAGCTGTGGTTGTGGGTACTGATGG
AGCAAAAATGAGTAAATCTTATCAAAATACTATAGATATTTTTAGCAGTGAAAAAACCTTAAAAAAACAAATTTCTTCCA
TAGTTACAGATAGTACCGCTTTAGAAGATCCAAAAGATCATGAAAACTGCAATATTTTTAAAATCGCAAAGCTTTTTTTA
GATGAATCAGGGCAAAAAGAATTACAAATACGCTATGAAAAAGGTGGTGAGGGTTATGGACATTTTAAAATATATTTAAA
CGAGCTTGTAAATGCTTATTTTAAAGAAGCAAGAGAAAAATATAATGAGCTTTTAGAAAAGCCTTCGCATTTGAAAGAAA
TTTTGGATTTTGGTGCGACAAAAGCAAGAAAAATCGCCCAAGAAAAAATGCAAAAAATTTATGAAAAAATCGGACTTTAA

Upstream 100 bases:

>100_bases
ATGAGCGTTTAAGCAAGTATGAACAAAAGGCAAATTTTATTTTAAATATAGAAAATAAAAATATAGATGAGCTTTTAAGC
GAGATTAAAAAGGTGATAAA

Downstream 100 bases:

>100_bases
GGAGTAGAAAATGCTAGATTTAAAAAATTTGCAAAATAATTTTGATGAAGTGGCTAAAAAGTTAAAAAATAAAAAAGTTG
ATGAAAATATCTTAAAAAAA

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRVLTGLQPSGDLHIGNYFGAIKQMVDAQEKSQMFMFIANYHAMTSSQDGEKLKQNSLKAAAAFLSLGIDPQKSVFWLQS
DVKEVMELYWILSQFTPMGLLERAHSYKDKVAKGLSASHGLFSYPVLMAADILLFDTRIVPVGKDQIQHVEIARDIALKV
NNEWGEIFTLPEARVNEEVAVVVGTDGAKMSKSYQNTIDIFSSEKTLKKQISSIVTDSTALEDPKDHENCNIFKIAKLFL
DESGQKELQIRYEKGGEGYGHFKIYLNELVNAYFKEAREKYNELLEKPSHLKEILDFGATKARKIAQEKMQKIYEKIGL

Sequences:

>Translated_319_residues
MRVLTGLQPSGDLHIGNYFGAIKQMVDAQEKSQMFMFIANYHAMTSSQDGEKLKQNSLKAAAAFLSLGIDPQKSVFWLQS
DVKEVMELYWILSQFTPMGLLERAHSYKDKVAKGLSASHGLFSYPVLMAADILLFDTRIVPVGKDQIQHVEIARDIALKV
NNEWGEIFTLPEARVNEEVAVVVGTDGAKMSKSYQNTIDIFSSEKTLKKQISSIVTDSTALEDPKDHENCNIFKIAKLFL
DESGQKELQIRYEKGGEGYGHFKIYLNELVNAYFKEAREKYNELLEKPSHLKEILDFGATKARKIAQEKMQKIYEKIGL
>Mature_319_residues
MRVLTGLQPSGDLHIGNYFGAIKQMVDAQEKSQMFMFIANYHAMTSSQDGEKLKQNSLKAAAAFLSLGIDPQKSVFWLQS
DVKEVMELYWILSQFTPMGLLERAHSYKDKVAKGLSASHGLFSYPVLMAADILLFDTRIVPVGKDQIQHVEIARDIALKV
NNEWGEIFTLPEARVNEEVAVVVGTDGAKMSKSYQNTIDIFSSEKTLKKQISSIVTDSTALEDPKDHENCNIFKIAKLFL
DESGQKELQIRYEKGGEGYGHFKIYLNELVNAYFKEAREKYNELLEKPSHLKEILDFGATKARKIAQEKMQKIYEKIGL

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=326, Percent_Identity=32.8220858895706, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI41352700, Length=182, Percent_Identity=41.7582417582418, Blast_Score=148, Evalue=6e-36,
Organism=Escherichia coli, GI1789786, Length=329, Percent_Identity=38.6018237082067, Blast_Score=207, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI71982800, Length=331, Percent_Identity=28.7009063444109, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI71982793, Length=330, Percent_Identity=27.5757575757576, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320474, Length=347, Percent_Identity=30.835734870317, Blast_Score=146, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24666151, Length=325, Percent_Identity=31.3846153846154, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_CAMJE (Q9PIB4)

Other databases:

- EMBL:   AL111168
- PIR:   B81382
- RefSeq:   YP_002343825.1
- PDB:   3M5W
- PDBsum:   3M5W
- ProteinModelPortal:   Q9PIB4
- SMR:   Q9PIB4
- IntAct:   Q9PIB4
- GeneID:   904711
- GenomeReviews:   AL111168_GR
- KEGG:   cje:Cj0388
- HOGENOM:   HBG293263
- OMA:   YFIADYH
- PhylomeDB:   Q9PIB4
- ProtClustDB:   PRK00927
- BioCyc:   CJEJ192222:CJ0388-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 36085; Mature: 36085

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLTGLQPSGDLHIGNYFGAIKQMVDAQEKSQMFMFIANYHAMTSSQDGEKLKQNSLKA
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHEEEHEEHHHHHCCCCCHHHHHHHHHHH
AAAFLSLGIDPQKSVFWLQSDVKEVMELYWILSQFTPMGLLERAHSYKDKVAKGLSASHG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
LFSYPVLMAADILLFDTRIVPVGKDQIQHVEIARDIALKVNNEWGEIFTLPEARVNEEVA
HHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHEEECCCCCCEEECCCCCCCCCEE
VVVGTDGAKMSKSYQNTIDIFSSEKTLKKQISSIVTDSTALEDPKDHENCNIFKIAKLFL
EEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
DESGQKELQIRYEKGGEGYGHFKIYLNELVNAYFKEAREKYNELLEKPSHLKEILDFGAT
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCH
KARKIAQEKMQKIYEKIGL
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVLTGLQPSGDLHIGNYFGAIKQMVDAQEKSQMFMFIANYHAMTSSQDGEKLKQNSLKA
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHEEEHEEHHHHHCCCCCHHHHHHHHHHH
AAAFLSLGIDPQKSVFWLQSDVKEVMELYWILSQFTPMGLLERAHSYKDKVAKGLSASHG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
LFSYPVLMAADILLFDTRIVPVGKDQIQHVEIARDIALKVNNEWGEIFTLPEARVNEEVA
HHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHEEECCCCCCEEECCCCCCCCCEE
VVVGTDGAKMSKSYQNTIDIFSSEKTLKKQISSIVTDSTALEDPKDHENCNIFKIAKLFL
EEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
DESGQKELQIRYEKGGEGYGHFKIYLNELVNAYFKEAREKYNELLEKPSHLKEILDFGAT
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCH
KARKIAQEKMQKIYEKIGL
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204