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The map label for this gene is hemN [H]

Identifier: 57237418

GI number: 57237418

Start: 367039

End: 368409

Strand: Reverse

Name: hemN [H]

Synonym: CJE0412

Alternate gene names: 57237418

Gene position: 368409-367039 (Counterclockwise)

Preceding gene: 57237420

Following gene: 57237414

Centisome position: 20.72

GC content: 28.52

Gene sequence:

>1371_bases
ATGAATTTATTTCAAAATTTAGCTTTAAAATACTCTCATACTATGATGGAAAAATCCCTTCAAAAAGGTTTCAATGTCGA
ACTTTTAAAACAACCCAAAGAAAAAATTCCAAAACAAGACAAAAGCTATATGCTTTATGCTCATGTACCTTTTTGCCATA
CCTTTTGTCCTTATTGTAGCTTTCATAAATATTATTATGATGAAAATTTAGCCAAAGTATATTTTCAAAATCTTAGAGAA
GAAATTAAAATCATGAAAGATAAAGGTTTTGATTTTACCTCCATGTATGTGGGTGGTGGTACAACACTGATTGATGAAGA
GGAATTATTAAAAACCTTAGAACTTTGTAAAAAGTTATTTAATATAAAAGAAATTTCATGCGAAAGCGATCCAAATCATA
TTGATCCAAATAAGCTTTCTATGTTTAAAGGTATAATTGATCGTTTAAGTTGCGGGATTCAAAGTTTTGATGATGAAACT
TTGAAAAAAGTGGCAAGATATAATAAATTTGGTTCATCTAAAGAACTTCAAGAAAAAATTTCAAAAGCTTTAGGAATTTT
ACCTATTTTTAGTATAGATCTTATTTTCAATCTTCCAGGACAAAACGAAAAACAACTTTTAAATGACTTAGAAATTGCTA
AAAATTTAGCCCCGCAACAAATCACAACCTATCCTTTAATGAAATCAAATCTTACCAAAGATAATATCGCTAAAAGTCTA
GGAGTGAGCATTAAAGATAATGAATTTACTTATTATCAAATCATACGAGAATTTTTTAAAGACTATACCCAAAACAATGG
TTGGAGTTTTTCTTTAGAAAAAAACAAGCTTAATGACGAATATGTCAGTTCTCATCATGAATACCTAGGAGTAGGCAGCG
GAGCTTTTAGCTTTTTAGATGGGGAGCTTTTAATCAATGCATTTAATCTAAATGATTATGCTAAATTTATACAAGAAAAA
CAAAATGCTAATATAGCCAAAGCTTGTTTTGATAAAAAAGAAATTATCAAATATGTCTTTTTAACTGAAATTTTTTCAGG
GAAAATAGAAATTGATAAATTTAATAAAACTCTAAATTGCAATCTCGAAAAAGAACTTTTCATAGAACTTTTAGGACTTA
AAACAAGTGGTGCCATCATAAAAGATCAAAATGCTTTAATCGCAACTGAATTTGGTCAATATCTTTTTGTGGTTTTAATG
AAAGATTTTTACACAGGAATGGATCTTGTGCGCGCAGTATTTAGAGATGATAAGCGTCTTAAAAATAAAGATTATATCGA
TATCATGAAAGAAAATATTGATCCGCTTAACTCCGCTAGTATGGAATTTAAAAGCGGAGACAAACTAAAAGGGACGAACA
ACCATCATTAA

Upstream 100 bases:

>100_bases
ACTTAATCCCATTTTTTATTTAAGAAATAAAAAATTTACTCCTATTTTAGCTAAAAATTGATAAAGTTATGTCTTAAATA
TTTTAATATATAGGCCACTT

Downstream 100 bases:

>100_bases
AGCGATGATAATAAACATTATAGTTGGAAATTCATTATAAATTCTAAAATATTTTCCACTTTTAGTAGAAGTATCATTTG
CTAAAGCTTTTAAACAATAA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MNLFQNLALKYSHTMMEKSLQKGFNVELLKQPKEKIPKQDKSYMLYAHVPFCHTFCPYCSFHKYYYDENLAKVYFQNLRE
EIKIMKDKGFDFTSMYVGGGTTLIDEEELLKTLELCKKLFNIKEISCESDPNHIDPNKLSMFKGIIDRLSCGIQSFDDET
LKKVARYNKFGSSKELQEKISKALGILPIFSIDLIFNLPGQNEKQLLNDLEIAKNLAPQQITTYPLMKSNLTKDNIAKSL
GVSIKDNEFTYYQIIREFFKDYTQNNGWSFSLEKNKLNDEYVSSHHEYLGVGSGAFSFLDGELLINAFNLNDYAKFIQEK
QNANIAKACFDKKEIIKYVFLTEIFSGKIEIDKFNKTLNCNLEKELFIELLGLKTSGAIIKDQNALIATEFGQYLFVVLM
KDFYTGMDLVRAVFRDDKRLKNKDYIDIMKENIDPLNSASMEFKSGDKLKGTNNHH

Sequences:

>Translated_456_residues
MNLFQNLALKYSHTMMEKSLQKGFNVELLKQPKEKIPKQDKSYMLYAHVPFCHTFCPYCSFHKYYYDENLAKVYFQNLRE
EIKIMKDKGFDFTSMYVGGGTTLIDEEELLKTLELCKKLFNIKEISCESDPNHIDPNKLSMFKGIIDRLSCGIQSFDDET
LKKVARYNKFGSSKELQEKISKALGILPIFSIDLIFNLPGQNEKQLLNDLEIAKNLAPQQITTYPLMKSNLTKDNIAKSL
GVSIKDNEFTYYQIIREFFKDYTQNNGWSFSLEKNKLNDEYVSSHHEYLGVGSGAFSFLDGELLINAFNLNDYAKFIQEK
QNANIAKACFDKKEIIKYVFLTEIFSGKIEIDKFNKTLNCNLEKELFIELLGLKTSGAIIKDQNALIATEFGQYLFVVLM
KDFYTGMDLVRAVFRDDKRLKNKDYIDIMKENIDPLNSASMEFKSGDKLKGTNNHH
>Mature_456_residues
MNLFQNLALKYSHTMMEKSLQKGFNVELLKQPKEKIPKQDKSYMLYAHVPFCHTFCPYCSFHKYYYDENLAKVYFQNLRE
EIKIMKDKGFDFTSMYVGGGTTLIDEEELLKTLELCKKLFNIKEISCESDPNHIDPNKLSMFKGIIDRLSCGIQSFDDET
LKKVARYNKFGSSKELQEKISKALGILPIFSIDLIFNLPGQNEKQLLNDLEIAKNLAPQQITTYPLMKSNLTKDNIAKSL
GVSIKDNEFTYYQIIREFFKDYTQNNGWSFSLEKNKLNDEYVSSHHEYLGVGSGAFSFLDGELLINAFNLNDYAKFIQEK
QNANIAKACFDKKEIIKYVFLTEIFSGKIEIDKFNKTLNCNLEKELFIELLGLKTSGAIIKDQNALIATEFGQYLFVVLM
KDFYTGMDLVRAVFRDDKRLKNKDYIDIMKENIDPLNSASMEFKSGDKLKGTNNHH

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789325, Length=277, Percent_Identity=24.5487364620939, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082341, Length=229, Percent_Identity=25.3275109170306, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 52768; Mature: 52768

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFQNLALKYSHTMMEKSLQKGFNVELLKQPKEKIPKQDKSYMLYAHVPFCHTFCPYCS
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHCCCCCCCEEEEEECCHHHHHHHHHH
FHKYYYDENLAKVYFQNLREEIKIMKDKGFDFTSMYVGGGTTLIDEEELLKTLELCKKLF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECHHHHHHHHHHHHHHH
NIKEISCESDPNHIDPNKLSMFKGIIDRLSCGIQSFDDETLKKVARYNKFGSSKELQEKI
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHH
SKALGILPIFSIDLIFNLPGQNEKQLLNDLEIAKNLAPQQITTYPLMKSNLTKDNIAKSL
HHHHCCHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHEECCHHHCCCCHHHHHHHH
GVSIKDNEFTYYQIIREFFKDYTQNNGWSFSLEKNKLNDEYVSSHHEYLGVGSGAFSFLD
CCEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCHHHEECCCCHHHHCC
GELLINAFNLNDYAKFIQEKQNANIAKACFDKKEIIKYVFLTEIFSGKIEIDKFNKTLNC
CCCEEEEECCHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCEECC
NLEKELFIELLGLKTSGAIIKDQNALIATEFGQYLFVVLMKDFYTGMDLVRAVFRDDKRL
CCHHHHHHHHHCCCCCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KNKDYIDIMKENIDPLNSASMEFKSGDKLKGTNNHH
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNLFQNLALKYSHTMMEKSLQKGFNVELLKQPKEKIPKQDKSYMLYAHVPFCHTFCPYCS
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHCCCCCCCEEEEEECCHHHHHHHHHH
FHKYYYDENLAKVYFQNLREEIKIMKDKGFDFTSMYVGGGTTLIDEEELLKTLELCKKLF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECHHHHHHHHHHHHHHH
NIKEISCESDPNHIDPNKLSMFKGIIDRLSCGIQSFDDETLKKVARYNKFGSSKELQEKI
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHH
SKALGILPIFSIDLIFNLPGQNEKQLLNDLEIAKNLAPQQITTYPLMKSNLTKDNIAKSL
HHHHCCHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHEECCHHHCCCCHHHHHHHH
GVSIKDNEFTYYQIIREFFKDYTQNNGWSFSLEKNKLNDEYVSSHHEYLGVGSGAFSFLD
CCEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCHHHEECCCCHHHHCC
GELLINAFNLNDYAKFIQEKQNANIAKACFDKKEIIKYVFLTEIFSGKIEIDKFNKTLNC
CCCEEEEECCHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCEECC
NLEKELFIELLGLKTSGAIIKDQNALIATEFGQYLFVVLMKDFYTGMDLVRAVFRDDKRL
CCHHHHHHHHHCCCCCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KNKDYIDIMKENIDPLNSASMEFKSGDKLKGTNNHH
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8757728; 8969508; 9384377; 1339421 [H]