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The map label for this gene is trpB

Identifier: 57237403

GI number: 57237403

Start: 356515

End: 357693

Strand: Direct

Name: trpB

Synonym: CJE0397

Alternate gene names: 57237403

Gene position: 356515-357693 (Clockwise)

Preceding gene: 57237402

Following gene: 57237404

Centisome position: 20.05

GC content: 35.03

Gene sequence:

>1179_bases
ATGAAAAAAGCGTATTATGGGGATTTTGGTGGGCAGTTTTTGCCTGAAAGTGCAATGTTTGCTTTAAATGAGCTAGAAGG
TGCTTTTTTGAAATTTTCTAAAGATAAACTCTTTAAAAAAGAATTAAACGAACTTTTAAAAACTTATGTAGGGCGTCCTA
CTCCACTTTATTTTGCTAGAAATTTAAGCAAAAAATATCAGCATGAAATTTATTTAAAACGCGAGGATTTAAATCATACC
GGAGCACATAAAATCAATAATGCCATCGCTCAAGCCCTTTTAGCAAAAAAAATGGGAAAGAAAAAAATCATAGCAGAAAC
AGGAGCAGGTCAACACGGGCTTGCAACAGCCACTGCAGCAGCACTTTTAGGACTTGAATGTGAAATTTATATGGGGGCAA
CCGATGTGCAAAGACAGGCTTTAAATGTTTATAAAATGGAGCTTTTGGGTGCTAAAATTCATGCAGTACAAAGTGGGCTT
AAAACTCTAAAAGAAGCTACTACTGCGGCTATTCAAGCTTGGGTTGGAGATATTAAAAATATTTTTTATGTGGTTGGAAG
TGCGGTAGGTCCTTATCCTTATCCTAAAATGGTAATGCATTTTCAAAGTATTATAGGAAAAGAATGCAAAATGCAACTTC
AAAAACTCAATAAAAAAGTAGATTATATTATCGCAGCTGTTGGGGGTGGAAGCAATGCAGCTGGGATTTTTTATGATTTT
ATCAAAGATGAAAATGTTAAACTTATAGGTATTGAAGCAGGGGGGCTTGGCATTGATACGCCTTATCATGCAGCGACTTT
AAATAAGGGAAAAACAGGCATTATTCACGGTATGAAAACCAAGGTTTTACAAGATGATTTAGGCAATATCTTGCCTGTAC
ATAGTGTTTCTGCAGGGCTTGATTATCCAGGAATTGGTCCTTTGCATGCTTTTTTATTTGAAAGCAAAAGAGCGCAATAT
CATGCTATAAGTGATGAAGAATGTATGCAGGCTTTAAAATTACTTTGTAAAGAAGAAGGTATTATTGCAGCGATTGAAAG
TTCGCATGCTTTGGCATTTTTAGAAAAACTTTGTCCAACTTTAAAAAAGAAAAGCGTGATAGTAGTAAATCTTTCAGGCA
GGGGAGATAAAGATATGCAAATGATTAGAGATTATAAAAAAGGGGTGATTTATGGTTGA

Upstream 100 bases:

>100_bases
AAACGGGAGCTAAAATTTTAGATCTTAATTCAAAACTTGAAGATGAAAAAGGCTTAAAAGATATCAACAAAATAAAACAA
ATTTTAAAGGAGTTGAAAAA

Downstream 100 bases:

>100_bases
TTTTAGAAAATTTTATAAAGAAAATGCAAATGTGGCTTATACGGTTTTAGGTTATCCAAATCTTCAAACAAGTGAAGCTT
TTTTGCAAAGATTGGATCAA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKKAYYGDFGGQFLPESAMFALNELEGAFLKFSKDKLFKKELNELLKTYVGRPTPLYFARNLSKKYQHEIYLKREDLNHT
GAHKINNAIAQALLAKKMGKKKIIAETGAGQHGLATATAAALLGLECEIYMGATDVQRQALNVYKMELLGAKIHAVQSGL
KTLKEATTAAIQAWVGDIKNIFYVVGSAVGPYPYPKMVMHFQSIIGKECKMQLQKLNKKVDYIIAAVGGGSNAAGIFYDF
IKDENVKLIGIEAGGLGIDTPYHAATLNKGKTGIIHGMKTKVLQDDLGNILPVHSVSAGLDYPGIGPLHAFLFESKRAQY
HAISDEECMQALKLLCKEEGIIAAIESSHALAFLEKLCPTLKKKSVIVVNLSGRGDKDMQMIRDYKKGVIYG

Sequences:

>Translated_392_residues
MKKAYYGDFGGQFLPESAMFALNELEGAFLKFSKDKLFKKELNELLKTYVGRPTPLYFARNLSKKYQHEIYLKREDLNHT
GAHKINNAIAQALLAKKMGKKKIIAETGAGQHGLATATAAALLGLECEIYMGATDVQRQALNVYKMELLGAKIHAVQSGL
KTLKEATTAAIQAWVGDIKNIFYVVGSAVGPYPYPKMVMHFQSIIGKECKMQLQKLNKKVDYIIAAVGGGSNAAGIFYDF
IKDENVKLIGIEAGGLGIDTPYHAATLNKGKTGIIHGMKTKVLQDDLGNILPVHSVSAGLDYPGIGPLHAFLFESKRAQY
HAISDEECMQALKLLCKEEGIIAAIESSHALAFLEKLCPTLKKKSVIVVNLSGRGDKDMQMIRDYKKGVIYG
>Mature_392_residues
MKKAYYGDFGGQFLPESAMFALNELEGAFLKFSKDKLFKKELNELLKTYVGRPTPLYFARNLSKKYQHEIYLKREDLNHT
GAHKINNAIAQALLAKKMGKKKIIAETGAGQHGLATATAAALLGLECEIYMGATDVQRQALNVYKMELLGAKIHAVQSGL
KTLKEATTAAIQAWVGDIKNIFYVVGSAVGPYPYPKMVMHFQSIIGKECKMQLQKLNKKVDYIIAAVGGGSNAAGIFYDF
IKDENVKLIGIEAGGLGIDTPYHAATLNKGKTGIIHGMKTKVLQDDLGNILPVHSVSAGLDYPGIGPLHAFLFESKRAQY
HAISDEECMQALKLLCKEEGIIAAIESSHALAFLEKLCPTLKKKSVIVVNLSGRGDKDMQMIRDYKKGVIYG

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=385, Percent_Identity=51.4285714285714, Blast_Score=397, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321412, Length=380, Percent_Identity=53.4210526315789, Blast_Score=415, Evalue=1e-117,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_CAMJ8 (A8FKD7)

Other databases:

- EMBL:   CP000814
- RefSeq:   YP_001481901.1
- ProteinModelPortal:   A8FKD7
- SMR:   A8FKD7
- STRING:   A8FKD7
- GeneID:   5617882
- GenomeReviews:   CP000814_GR
- KEGG:   cju:C8J_0325
- eggNOG:   COG0133
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   CJEJ407148:C8J_0325-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 42948; Mature: 42948

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAYYGDFGGQFLPESAMFALNELEGAFLKFSKDKLFKKELNELLKTYVGRPTPLYFAR
CCCCEECCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
NLSKKYQHEIYLKREDLNHTGAHKINNAIAQALLAKKMGKKKIIAETGAGQHGLATATAA
HHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
ALLGLECEIYMGATDVQRQALNVYKMELLGAKIHAVQSGLKTLKEATTAAIQAWVGDIKN
HHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFYVVGSAVGPYPYPKMVMHFQSIIGKECKMQLQKLNKKVDYIIAAVGGGSNAAGIFYDF
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHH
IKDENVKLIGIEAGGLGIDTPYHAATLNKGKTGIIHGMKTKVLQDDLGNILPVHSVSAGL
HCCCCEEEEEEECCCCCCCCCCCCEECCCCCCEEEECHHHHHHHHHHCCCCCCHHHHCCC
DYPGIGPLHAFLFESKRAQYHAISDEECMQALKLLCKEEGIIAAIESSHALAFLEKLCPT
CCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
LKKKSVIVVNLSGRGDKDMQMIRDYKKGVIYG
HCCCCEEEEEECCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKAYYGDFGGQFLPESAMFALNELEGAFLKFSKDKLFKKELNELLKTYVGRPTPLYFAR
CCCCEECCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
NLSKKYQHEIYLKREDLNHTGAHKINNAIAQALLAKKMGKKKIIAETGAGQHGLATATAA
HHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
ALLGLECEIYMGATDVQRQALNVYKMELLGAKIHAVQSGLKTLKEATTAAIQAWVGDIKN
HHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFYVVGSAVGPYPYPKMVMHFQSIIGKECKMQLQKLNKKVDYIIAAVGGGSNAAGIFYDF
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHH
IKDENVKLIGIEAGGLGIDTPYHAATLNKGKTGIIHGMKTKVLQDDLGNILPVHSVSAGL
HCCCCEEEEEEECCCCCCCCCCCCEECCCCCCEEEECHHHHHHHHHHCCCCCCHHHHCCC
DYPGIGPLHAFLFESKRAQYHAISDEECMQALKLLCKEEGIIAAIESSHALAFLEKLCPT
CCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
LKKKSVIVVNLSGRGDKDMQMIRDYKKGVIYG
HCCCCEEEEEECCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA