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The map label for this gene is trpA

Identifier: 57237404

GI number: 57237404

Start: 357686

End: 358435

Strand: Direct

Name: trpA

Synonym: CJE0398

Alternate gene names: 57237404

Gene position: 357686-358435 (Clockwise)

Preceding gene: 57237403

Following gene: 57237405

Centisome position: 20.12

GC content: 29.6

Gene sequence:

>750_bases
ATGGTTGATTTTAGAAAATTTTATAAAGAAAATGCAAATGTGGCTTATACGGTTTTAGGTTATCCAAATCTTCAAACAAG
TGAAGCTTTTTTGCAAAGATTGGATCAAAGCCCTATTGATATTTTAGAACTTGGTGTTGCTTATAGTGATCCTATTGCCG
ATGGTGAGATTATTGCAGATGCGGCAAAAATAGCTCTTGATCAAGGCGTGGATATTCATAGTGTTTTTGAGCTTTTAGCT
AGGATAAAAACTAAAAAAGCTTTGGTTTTTATGGTTTATTATAATTTAATTTTTTCTTATGGCTTGGAAAAATTTGTGAA
AAAAGCAAAATCTTTAGGGATTTGTGCTTTGATTGTGCCTGAATTGAGTTTTGAAGAAAGTGATGATTTAATTAAAGAGT
GTGAAAGATATAATATAGCTTTAATTACACTTGTAAGTGTTACAACCCCTAAAGAAAGGGTTAAAAAGTTAGTAAAACAT
GCTAAAGGTTTTATTTATTTATTAGCAAGCATAGGTATTACAGGTACGAAAAGTGTCGAAGAAGCAATCTTACAAGATAA
AGTAAAAGAAATCAGATCTTTTACAAATTTGCCTATTTTTGTAGGCTTTGGCATTCAAAATAATCAAGATGTAAAAAGAA
TGAGAAAAGTTGCTGACGGAGTGATTGTGGGAACTAGCATAGTAAAGTGTTTTAAACAAGGAAATTTGGATATAATAATG
AAAGATATTGAAGAAATTTTCAAAAAATAA

Upstream 100 bases:

>100_bases
TGTCCAACTTTAAAAAAGAAAAGCGTGATAGTAGTAAATCTTTCAGGCAGGGGAGATAAAGATATGCAAATGATTAGAGA
TTATAAAAAAGGGGTGATTT

Downstream 100 bases:

>100_bases
AATTTTAAAACATATTTTTATTTTATATTTACTATAATTTTAAATTAAGTTTTTTATAAAGGTGGTAGTTATGTTAAAGA
TTCTTGAGTATTCCATCACT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MVDFRKFYKENANVAYTVLGYPNLQTSEAFLQRLDQSPIDILELGVAYSDPIADGEIIADAAKIALDQGVDIHSVFELLA
RIKTKKALVFMVYYNLIFSYGLEKFVKKAKSLGICALIVPELSFEESDDLIKECERYNIALITLVSVTTPKERVKKLVKH
AKGFIYLLASIGITGTKSVEEAILQDKVKEIRSFTNLPIFVGFGIQNNQDVKRMRKVADGVIVGTSIVKCFKQGNLDIIM
KDIEEIFKK

Sequences:

>Translated_249_residues
MVDFRKFYKENANVAYTVLGYPNLQTSEAFLQRLDQSPIDILELGVAYSDPIADGEIIADAAKIALDQGVDIHSVFELLA
RIKTKKALVFMVYYNLIFSYGLEKFVKKAKSLGICALIVPELSFEESDDLIKECERYNIALITLVSVTTPKERVKKLVKH
AKGFIYLLASIGITGTKSVEEAILQDKVKEIRSFTNLPIFVGFGIQNNQDVKRMRKVADGVIVGTSIVKCFKQGNLDIIM
KDIEEIFKK
>Mature_249_residues
MVDFRKFYKENANVAYTVLGYPNLQTSEAFLQRLDQSPIDILELGVAYSDPIADGEIIADAAKIALDQGVDIHSVFELLA
RIKTKKALVFMVYYNLIFSYGLEKFVKKAKSLGICALIVPELSFEESDDLIKECERYNIALITLVSVTTPKERVKKLVKH
AKGFIYLLASIGITGTKSVEEAILQDKVKEIRSFTNLPIFVGFGIQNNQDVKRMRKVADGVIVGTSIVKCFKQGNLDIIM
KDIEEIFKK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=229, Percent_Identity=29.2576419213974, Blast_Score=116, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321412, Length=231, Percent_Identity=32.034632034632, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_CAMJ8 (A8FKD8)

Other databases:

- EMBL:   CP000814
- RefSeq:   YP_001481902.1
- ProteinModelPortal:   A8FKD8
- SMR:   A8FKD8
- STRING:   A8FKD8
- GeneID:   5617883
- GenomeReviews:   CP000814_GR
- KEGG:   cju:C8J_0326
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   CJEJ407148:C8J_0326-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27967; Mature: 27967

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 43-43 ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDFRKFYKENANVAYTVLGYPNLQTSEAFLQRLDQSPIDILELGVAYSDPIADGEIIAD
CCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCHHHHH
AAKIALDQGVDIHSVFELLARIKTKKALVFMVYYNLIFSYGLEKFVKKAKSLGICALIVP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
ELSFEESDDLIKECERYNIALITLVSVTTPKERVKKLVKHAKGFIYLLASIGITGTKSVE
CCCCCCCHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
EAILQDKVKEIRSFTNLPIFVGFGIQNNQDVKRMRKVADGVIVGTSIVKCFKQGNLDIIM
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHH
KDIEEIFKK
HHHHHHHCC
>Mature Secondary Structure
MVDFRKFYKENANVAYTVLGYPNLQTSEAFLQRLDQSPIDILELGVAYSDPIADGEIIAD
CCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCHHHHH
AAKIALDQGVDIHSVFELLARIKTKKALVFMVYYNLIFSYGLEKFVKKAKSLGICALIVP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
ELSFEESDDLIKECERYNIALITLVSVTTPKERVKKLVKHAKGFIYLLASIGITGTKSVE
CCCCCCCHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
EAILQDKVKEIRSFTNLPIFVGFGIQNNQDVKRMRKVADGVIVGTSIVKCFKQGNLDIIM
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHH
KDIEEIFKK
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA