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The map label for this gene is polA [H]

Identifier: 57237389

GI number: 57237389

Start: 341564

End: 344203

Strand: Reverse

Name: polA [H]

Synonym: CJE0383

Alternate gene names: 57237389

Gene position: 344203-341564 (Counterclockwise)

Preceding gene: 57237392

Following gene: 57237388

Centisome position: 19.36

GC content: 29.28

Gene sequence:

>2640_bases
ATGAAAACTTTAACTATTATTGATACTTTTGGATTTTTTTTCAGACTTTACTACGCATTAAAAGATTTTAAAAATTCACA
AGGACAAGCTAGTGGAATGATTAGCGGTTTTGCAAATTTTATTTATAGCTTAAAAAATGAACATAAAAGTGATTATATTG
TTTTTGCATTAGATTCCAAAGGAAAAACTTTTCGTAGTGAAATTGATCCAAACTATAAACAAAATCGCACTCCACCACCG
CCTGAACTTCTAGAACAAATTCCAATTTGCATAGAAATGATAGAAAAAATGGGTTTTATAAGCGTTTCTCAAGAAGGTTA
TGAAGCTGATGATATCATCGCTTCTTTTGTAAAAACTTGTGAAGATAGGGATATTTTTGTACGCATTATCACTCAAGATA
AAGATCTTTATCAACTCATAAAAGATGGTAAAACAAGCATTTACAGTCCTATTTCTAAAAACGACTATGATGAAGCAGCG
TGTTTAGAAAAATACGGAGTAAAACCTAATCAAATCAGAGATTTTTTAGCTCTTTGTGGGGATAGTTCTGATAATATTCC
AGGCGTTAAAGGTATAGGTGCAAAAGGAGCTAAAACCTTGCTTGATGAGTTTGGTAGCATAGAGGGAATTTATGAAAATT
TAACCCTAGTACGTAACGAAAGAAGTCGTAACTTGCTTTTAGAGAGTAAGGAAAATGCATTTTTAAGCAAAAAATTAGCT
TCTTTGTATGACAATTTAGAAGTACAAAATCTCATAGAAAAAGCCACTTATCCTGATGAAGAACCTCTTTTAAAAATACT
TGAAATTTTAGAACATTACGAACTTAATACTTTGCTTAAAAAACTGCGTCAAAATCCTGATAATAAAGATAAAAATTTAG
GTTTTAAAGCCACTTTAGTACAAGATGAAAACAAACTTTTTGAAATTTTAAATACCCTTGATAAAGAAAGTATTATTGCT
TTTGATACCGAAACCACAGGACTTGATACTAAAGAAGCAAAAATTGTTGGTTTTAGTTTTTGCATGAATGAAAATGAAGC
CTTTTACGTGCCTTTAACTCATAATTATCTTGGAGTAGGTGAGCAAATCTCATTGCAAAGTGCCAAAAAAGCTATAGAAG
TAATCTTTAATCATTTTGTTATAGGGCACAATCTCAAATATGATTTTAAAATCATTCAAAACAATTTTGACTTAAATTTA
CCTCAAAAATACGCAGATACAATGATTTTAGCATGGCTTAAAAATCCTTCTTTAAGAGTAAATATGGATGATTTAGCCTT
AAGACTTTTTAATTACGAAACCTTGCATTTTGAAAGCCTTGTTAAAAAAGGAGAAAATTTTGCAAGTGTAGAACTTGAAA
AAGCTTGTAAATACGCTGCAGAAGATGCTTATATCACTTTAAGATTTTATCTTTATTTTTTAAAAAATCTTGAAACTCCT
TTATTAGAACTTGCTAAAAACTGTGAATTTGATTTCATTAAAATCATTATGATGATGGAAGAAAATGGCATAAAGCTTGA
TACAAATGCACTTGAAATCCTTATGAAAAAATTTGAAAATGAAATCAAAAATTTAAGTGAAGAAATTTATACTCTTTGTG
AGGATAGATTCAACCTAAATTCACCTAAACAAGTAGGTGATATACTTTTTGAAAAACTCAAACTCCCAAGTAGAAAAAAA
GGTAAAACAGGATATTCTACCGATGAAAAAGTTTTAAATGCACTTTTAGATAAACATCCTGCGATTACCAAAATTTTAGA
TTATAGGGAACTTGCCAAGCTTTATTCCACTTATTGCGAACCTTTGTTAAAACTAGCATTAAAAGATAAAAATTCACGCA
TTTATTCAAGTTTTTTACAAACTGGAACCGCTACAGGACGCCTTTCATCTAAGGATCCAAATTTGCAAAATATTCCTGCA
CACGGACAATACGCAAAAGATTATAAATCTTGCTTTGTAGCTAAAGATGGATTTAGTTTTATCTCTCTTGATTATTCTCA
AATCGAACTGAGAATGCTTGCACATTTTAGCGAAGATGAAAAACTTTTAAATGCTTTTGCCAACGATGAAGATATACATG
CAAGAACAGCTATAATGATTTTTGGAGAAAGCAATTATGAAACCAGAAGTGTTGCAAAAAGTATAAATTTTGGACTTATT
TATGGCATGGGCTATAAAACTTTAAGTCAAAATTTAAAAATAGAAGCTCATTTAGCCAAATCTTACATAGAAAAATATTT
TGAAAATTTTACTAGTATTAAAAAATATTTTGAAACAGTTAAAAACGAAGCTAAACAAAATGGCTTTATCACTACTTTAA
GCGGTCGCAAGCGTTATTTTGATTTTGAAAACGCAAAACCTATGCAAATTGCCATGTATGAAAGAGAAAGCATTAATTCA
ATCTTACAAGGCTCAGCGGCTGATGTAATCAAACTTGCAATGCTAGAAATCAATAAAGAATTAAATGAAGACAAAAAGCT
TATTTTGCAAATTCACGATGAATTAATTTTTGAAGTAAAAGACGATTTGTGTGAAAATTTTGTAAAAAAAACTAGAGATA
TTATGGAAAATATAGTAAAATTAAAAGTAAAATTAAAAACAAGTTCAAGTATCGCCAAAAATTGGGGCGATTTAAAATAA

Upstream 100 bases:

>100_bases
GAGTATTTTCGCTTAAATATCTTTAAATTTCGAGACATTTTAAGCAAAATTTTGAAATAATATAACCAAAAAATATATTT
TATCAAAAAAAGAAATTATT

Downstream 100 bases:

>100_bases
TCAAGGAGATAAATTAATGGATCTTTCAACCATATTAGGAATGGTGCTTGCAGTTACTAGTATTTCGGTAGGAGATATAC
TAGAGGGAGGCAATCCTTTG

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 879; Mature: 879

Protein sequence:

>879_residues
MKTLTIIDTFGFFFRLYYALKDFKNSQGQASGMISGFANFIYSLKNEHKSDYIVFALDSKGKTFRSEIDPNYKQNRTPPP
PELLEQIPICIEMIEKMGFISVSQEGYEADDIIASFVKTCEDRDIFVRIITQDKDLYQLIKDGKTSIYSPISKNDYDEAA
CLEKYGVKPNQIRDFLALCGDSSDNIPGVKGIGAKGAKTLLDEFGSIEGIYENLTLVRNERSRNLLLESKENAFLSKKLA
SLYDNLEVQNLIEKATYPDEEPLLKILEILEHYELNTLLKKLRQNPDNKDKNLGFKATLVQDENKLFEILNTLDKESIIA
FDTETTGLDTKEAKIVGFSFCMNENEAFYVPLTHNYLGVGEQISLQSAKKAIEVIFNHFVIGHNLKYDFKIIQNNFDLNL
PQKYADTMILAWLKNPSLRVNMDDLALRLFNYETLHFESLVKKGENFASVELEKACKYAAEDAYITLRFYLYFLKNLETP
LLELAKNCEFDFIKIIMMMEENGIKLDTNALEILMKKFENEIKNLSEEIYTLCEDRFNLNSPKQVGDILFEKLKLPSRKK
GKTGYSTDEKVLNALLDKHPAITKILDYRELAKLYSTYCEPLLKLALKDKNSRIYSSFLQTGTATGRLSSKDPNLQNIPA
HGQYAKDYKSCFVAKDGFSFISLDYSQIELRMLAHFSEDEKLLNAFANDEDIHARTAIMIFGESNYETRSVAKSINFGLI
YGMGYKTLSQNLKIEAHLAKSYIEKYFENFTSIKKYFETVKNEAKQNGFITTLSGRKRYFDFENAKPMQIAMYERESINS
ILQGSAADVIKLAMLEINKELNEDKKLILQIHDELIFEVKDDLCENFVKKTRDIMENIVKLKVKLKTSSSIAKNWGDLK

Sequences:

>Translated_879_residues
MKTLTIIDTFGFFFRLYYALKDFKNSQGQASGMISGFANFIYSLKNEHKSDYIVFALDSKGKTFRSEIDPNYKQNRTPPP
PELLEQIPICIEMIEKMGFISVSQEGYEADDIIASFVKTCEDRDIFVRIITQDKDLYQLIKDGKTSIYSPISKNDYDEAA
CLEKYGVKPNQIRDFLALCGDSSDNIPGVKGIGAKGAKTLLDEFGSIEGIYENLTLVRNERSRNLLLESKENAFLSKKLA
SLYDNLEVQNLIEKATYPDEEPLLKILEILEHYELNTLLKKLRQNPDNKDKNLGFKATLVQDENKLFEILNTLDKESIIA
FDTETTGLDTKEAKIVGFSFCMNENEAFYVPLTHNYLGVGEQISLQSAKKAIEVIFNHFVIGHNLKYDFKIIQNNFDLNL
PQKYADTMILAWLKNPSLRVNMDDLALRLFNYETLHFESLVKKGENFASVELEKACKYAAEDAYITLRFYLYFLKNLETP
LLELAKNCEFDFIKIIMMMEENGIKLDTNALEILMKKFENEIKNLSEEIYTLCEDRFNLNSPKQVGDILFEKLKLPSRKK
GKTGYSTDEKVLNALLDKHPAITKILDYRELAKLYSTYCEPLLKLALKDKNSRIYSSFLQTGTATGRLSSKDPNLQNIPA
HGQYAKDYKSCFVAKDGFSFISLDYSQIELRMLAHFSEDEKLLNAFANDEDIHARTAIMIFGESNYETRSVAKSINFGLI
YGMGYKTLSQNLKIEAHLAKSYIEKYFENFTSIKKYFETVKNEAKQNGFITTLSGRKRYFDFENAKPMQIAMYERESINS
ILQGSAADVIKLAMLEINKELNEDKKLILQIHDELIFEVKDDLCENFVKKTRDIMENIVKLKVKLKTSSSIAKNWGDLK
>Mature_879_residues
MKTLTIIDTFGFFFRLYYALKDFKNSQGQASGMISGFANFIYSLKNEHKSDYIVFALDSKGKTFRSEIDPNYKQNRTPPP
PELLEQIPICIEMIEKMGFISVSQEGYEADDIIASFVKTCEDRDIFVRIITQDKDLYQLIKDGKTSIYSPISKNDYDEAA
CLEKYGVKPNQIRDFLALCGDSSDNIPGVKGIGAKGAKTLLDEFGSIEGIYENLTLVRNERSRNLLLESKENAFLSKKLA
SLYDNLEVQNLIEKATYPDEEPLLKILEILEHYELNTLLKKLRQNPDNKDKNLGFKATLVQDENKLFEILNTLDKESIIA
FDTETTGLDTKEAKIVGFSFCMNENEAFYVPLTHNYLGVGEQISLQSAKKAIEVIFNHFVIGHNLKYDFKIIQNNFDLNL
PQKYADTMILAWLKNPSLRVNMDDLALRLFNYETLHFESLVKKGENFASVELEKACKYAAEDAYITLRFYLYFLKNLETP
LLELAKNCEFDFIKIIMMMEENGIKLDTNALEILMKKFENEIKNLSEEIYTLCEDRFNLNSPKQVGDILFEKLKLPSRKK
GKTGYSTDEKVLNALLDKHPAITKILDYRELAKLYSTYCEPLLKLALKDKNSRIYSSFLQTGTATGRLSSKDPNLQNIPA
HGQYAKDYKSCFVAKDGFSFISLDYSQIELRMLAHFSEDEKLLNAFANDEDIHARTAIMIFGESNYETRSVAKSINFGLI
YGMGYKTLSQNLKIEAHLAKSYIEKYFENFTSIKKYFETVKNEAKQNGFITTLSGRKRYFDFENAKPMQIAMYERESINS
ILQGSAADVIKLAMLEINKELNEDKKLILQIHDELIFEVKDDLCENFVKKTRDIMENIVKLKVKLKTSSSIAKNWGDLK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=439, Percent_Identity=32.1184510250569, Blast_Score=217, Evalue=5e-56,
Organism=Homo sapiens, GI139394648, Length=625, Percent_Identity=27.04, Blast_Score=194, Evalue=4e-49,
Organism=Escherichia coli, GI1790294, Length=920, Percent_Identity=35.6521739130435, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI226510970, Length=201, Percent_Identity=34.8258706467662, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI86563272, Length=449, Percent_Identity=28.5077951002227, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI17933644, Length=592, Percent_Identity=28.2094594594595, Blast_Score=202, Evalue=8e-52,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 100965; Mature: 100965

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLTIIDTFGFFFRLYYALKDFKNSQGQASGMISGFANFIYSLKNEHKSDYIVFALDSK
CCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
GKTFRSEIDPNYKQNRTPPPPELLEQIPICIEMIEKMGFISVSQEGYEADDIIASFVKTC
CCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHC
EDRDIFVRIITQDKDLYQLIKDGKTSIYSPISKNDYDEAACLEKYGVKPNQIRDFLALCG
CCCCEEEEEEECCHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHC
DSSDNIPGVKGIGAKGAKTLLDEFGSIEGIYENLTLVRNERSRNLLLESKENAFLSKKLA
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHH
SLYDNLEVQNLIEKATYPDEEPLLKILEILEHYELNTLLKKLRQNPDNKDKNLGFKATLV
HHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
QDENKLFEILNTLDKESIIAFDTETTGLDTKEAKIVGFSFCMNENEAFYVPLTHNYLGVG
CCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCC
EQISLQSAKKAIEVIFNHFVIGHNLKYDFKIIQNNFDLNLPQKYADTMILAWLKNPSLRV
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHEECCCCEEE
NMDDLALRLFNYETLHFESLVKKGENFASVELEKACKYAAEDAYITLRFYLYFLKNLETP
EHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCCEEHHHHHHHHHHCCCHH
LLELAKNCEFDFIKIIMMMEENGIKLDTNALEILMKKFENEIKNLSEEIYTLCEDRFNLN
HHHHHHCCCHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPKQVGDILFEKLKLPSRKKGKTGYSTDEKVLNALLDKHPAITKILDYRELAKLYSTYCE
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
PLLKLALKDKNSRIYSSFLQTGTATGRLSSKDPNLQNIPAHGQYAKDYKSCFVAKDGFSF
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCE
ISLDYSQIELRMLAHFSEDEKLLNAFANDEDIHARTAIMIFGESNYETRSVAKSINFGLI
EEECHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHCCCEEE
YGMGYKTLSQNLKIEAHLAKSYIEKYFENFTSIKKYFETVKNEAKQNGFITTLSGRKRYF
EECCHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEC
DFENAKPMQIAMYERESINSILQGSAADVIKLAMLEINKELNEDKKLILQIHDELIFEVK
CCCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
DDLCENFVKKTRDIMENIVKLKVKLKTSSSIAKNWGDLK
HHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHCCCCCC
>Mature Secondary Structure
MKTLTIIDTFGFFFRLYYALKDFKNSQGQASGMISGFANFIYSLKNEHKSDYIVFALDSK
CCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
GKTFRSEIDPNYKQNRTPPPPELLEQIPICIEMIEKMGFISVSQEGYEADDIIASFVKTC
CCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHC
EDRDIFVRIITQDKDLYQLIKDGKTSIYSPISKNDYDEAACLEKYGVKPNQIRDFLALCG
CCCCEEEEEEECCHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHC
DSSDNIPGVKGIGAKGAKTLLDEFGSIEGIYENLTLVRNERSRNLLLESKENAFLSKKLA
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHH
SLYDNLEVQNLIEKATYPDEEPLLKILEILEHYELNTLLKKLRQNPDNKDKNLGFKATLV
HHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
QDENKLFEILNTLDKESIIAFDTETTGLDTKEAKIVGFSFCMNENEAFYVPLTHNYLGVG
CCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCC
EQISLQSAKKAIEVIFNHFVIGHNLKYDFKIIQNNFDLNLPQKYADTMILAWLKNPSLRV
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHEECCCCEEE
NMDDLALRLFNYETLHFESLVKKGENFASVELEKACKYAAEDAYITLRFYLYFLKNLETP
EHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCCEEHHHHHHHHHHCCCHH
LLELAKNCEFDFIKIIMMMEENGIKLDTNALEILMKKFENEIKNLSEEIYTLCEDRFNLN
HHHHHHCCCHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPKQVGDILFEKLKLPSRKKGKTGYSTDEKVLNALLDKHPAITKILDYRELAKLYSTYCE
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
PLLKLALKDKNSRIYSSFLQTGTATGRLSSKDPNLQNIPAHGQYAKDYKSCFVAKDGFSF
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCE
ISLDYSQIELRMLAHFSEDEKLLNAFANDEDIHARTAIMIFGESNYETRSVAKSINFGLI
EEECHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHCCCEEE
YGMGYKTLSQNLKIEAHLAKSYIEKYFENFTSIKKYFETVKNEAKQNGFITTLSGRKRYF
EECCHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEC
DFENAKPMQIAMYERESINSILQGSAADVIKLAMLEINKELNEDKKLILQIHDELIFEVK
CCCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
DDLCENFVKKTRDIMENIVKLKVKLKTSSSIAKNWGDLK
HHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]