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The map label for this gene is fliF [H]

Identifier: 57237369

GI number: 57237369

Start: 324291

End: 325973

Strand: Direct

Name: fliF [H]

Synonym: CJE0363

Alternate gene names: 57237369

Gene position: 324291-325973 (Clockwise)

Preceding gene: 57237368

Following gene: 57237370

Centisome position: 18.24

GC content: 33.33

Gene sequence:

>1683_bases
ATGGATTTTAAAAATATGCTTCATCAAATTGGGCAACTTTATCAAAATTTAACTCGCAAGCAGCGTATAGTGATTGCTGC
ATCTATTGTAGTAGTGGTTGGGTTTTTGGTTTTTTTAGCTTTATTTCGTGGAAGTGGTAGTAATGCAAATAATGGATATG
CAGTTTTAGTTGAAAATGTAAGCCCAAGTTCTTCTGCTGCAATTGTAGCTAAACTTGAACAAAATAATATTCCCTATAAA
TTAGAAAGTGAAAATAAAATTTTAGTTCCACAAGATCAGGTTTATCGTCAAAGAATGTTTATTGCAAGCGAAGGTCTTAT
AAAAGATAGTCGCGTGGGTTTTGAAGCTTTTGATACTCAAGCTTTTGGAGCTACAAATGAAGAGCAAAAGGTAAAATACC
AAAGAGCCATAGAAGGTGAACTTGCAAGAACTATTGAGACTTTAGAACCTATTCGTAGTGCTGTTGTACATATAGCATTT
CCTAAAGATAGTGTTTTTACCGAAAGGCAAATTCCGCCTACAGCTTCAGTTGTTGTTAATGTTCGCGAAGGCTTAAAGCT
TACTAGAAAACAAATTGATGGTATAAAAAATATTGTTTCAGCGGCAGTTCCAAAACTTACTAAAGAAAATGTTAAGATTA
GTGATCAAAGTGGTGTGCCTTTAGATGAGCAAGAAGCATATGAAGATGACTTGGTTCGTGCGCAAATTAAATTTAAAAGT
GATCAAGAAAAAGCTTTAGAAGATAAGATTATAGAAAATTTAGCTCCTTTTGCAGGTGGCATGGATAAAGTAAAAGTGAG
TGTAAATATAGATTTTGATTTTTCCAAGCAAGAATCTCAAAGTGAAATTTATGATCCAAATCCTATAGTGCGTAGTGAGC
AAACTTTAAACGAGGAAAGAACAGGTAGAAAAGATCCAGAAATTCAGGGTGTTCCAGGTGCTGTTTCTAATATAGGTCCT
GTTGAAGGACTTGACAATAAAGGTCAAATTGACACTTATAAAAAAAATCAAGTTACTACAAACAATGAACTTTCTAAAAC
TATCACAAATACAAAAAAACAATTTGCAACAGTTTTACGCACTTCAGCAGCTGTCACTATAGATGGAAAATATCAAGATG
TGGTTGATGAAAATGGGGATGTGAAAAGCGAATATGTTCCATTGACTAAAGAAGAGCTTGCAAGTGTTGAGAGTATAGTT
AAAAATACTATTAATTTTAGCGCTACTAGAGGTGATAGTGTTGTAGTGCAAAATTTACCATTTCATCGTGAGTCGATTAG
AGTTGAGAGTAAAGTTAAAACTTTTTACAATCGTTTTGTTGAGCCATTTATTCCACCAGTTAAGTATTTTATTGCAGCGA
TTTTACTTTTTATTTTTTACAAAAAAGTCATTGCTCCATTTACTCAAAAAATGCTTGAAGATGTCGCAGCGCAAGAAGAA
ATGCAGCAAGGTCCAAATGCTGTTTTAGATGATGCTGAAGATGCTTTGGAGAAATTTAATGCAGCAAGGAAAAAAGTTGA
AGAACAACTTGGTTTTGGGGATAATTTTAATGAAGATTCTATTCAATATGAGGTTTTACTTGAAAAATTGCGTGGTTTAG
TAAGTGATAAAGGCGAGGAAATTGCAGCACTTTTACAAAACTTAATTCAAAATGATAGTGAATTTGGCGAAAAGGATATG
TGA

Upstream 100 bases:

>100_bases
TTATGAAAATGAAAAATTTTTTACAGAATTTGATAAGATTTTAAGATAGATAAATCAAATAAAAATATGTAAAATTCGCT
AAACTAAGGGATGAGAATTA

Downstream 100 bases:

>100_bases
TGATAAAGCTAAGTGAAGAACAAAAAATGGTCTATGATGATTTATCGATGCCTGAGAAGGTAGCGATATTTCTTATACAA
CTTGGCGAAGATGCAACAAC

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MDFKNMLHQIGQLYQNLTRKQRIVIAASIVVVVGFLVFLALFRGSGSNANNGYAVLVENVSPSSSAAIVAKLEQNNIPYK
LESENKILVPQDQVYRQRMFIASEGLIKDSRVGFEAFDTQAFGATNEEQKVKYQRAIEGELARTIETLEPIRSAVVHIAF
PKDSVFTERQIPPTASVVVNVREGLKLTRKQIDGIKNIVSAAVPKLTKENVKISDQSGVPLDEQEAYEDDLVRAQIKFKS
DQEKALEDKIIENLAPFAGGMDKVKVSVNIDFDFSKQESQSEIYDPNPIVRSEQTLNEERTGRKDPEIQGVPGAVSNIGP
VEGLDNKGQIDTYKKNQVTTNNELSKTITNTKKQFATVLRTSAAVTIDGKYQDVVDENGDVKSEYVPLTKEELASVESIV
KNTINFSATRGDSVVVQNLPFHRESIRVESKVKTFYNRFVEPFIPPVKYFIAAILLFIFYKKVIAPFTQKMLEDVAAQEE
MQQGPNAVLDDAEDALEKFNAARKKVEEQLGFGDNFNEDSIQYEVLLEKLRGLVSDKGEEIAALLQNLIQNDSEFGEKDM

Sequences:

>Translated_560_residues
MDFKNMLHQIGQLYQNLTRKQRIVIAASIVVVVGFLVFLALFRGSGSNANNGYAVLVENVSPSSSAAIVAKLEQNNIPYK
LESENKILVPQDQVYRQRMFIASEGLIKDSRVGFEAFDTQAFGATNEEQKVKYQRAIEGELARTIETLEPIRSAVVHIAF
PKDSVFTERQIPPTASVVVNVREGLKLTRKQIDGIKNIVSAAVPKLTKENVKISDQSGVPLDEQEAYEDDLVRAQIKFKS
DQEKALEDKIIENLAPFAGGMDKVKVSVNIDFDFSKQESQSEIYDPNPIVRSEQTLNEERTGRKDPEIQGVPGAVSNIGP
VEGLDNKGQIDTYKKNQVTTNNELSKTITNTKKQFATVLRTSAAVTIDGKYQDVVDENGDVKSEYVPLTKEELASVESIV
KNTINFSATRGDSVVVQNLPFHRESIRVESKVKTFYNRFVEPFIPPVKYFIAAILLFIFYKKVIAPFTQKMLEDVAAQEE
MQQGPNAVLDDAEDALEKFNAARKKVEEQLGFGDNFNEDSIQYEVLLEKLRGLVSDKGEEIAALLQNLIQNDSEFGEKDM
>Mature_560_residues
MDFKNMLHQIGQLYQNLTRKQRIVIAASIVVVVGFLVFLALFRGSGSNANNGYAVLVENVSPSSSAAIVAKLEQNNIPYK
LESENKILVPQDQVYRQRMFIASEGLIKDSRVGFEAFDTQAFGATNEEQKVKYQRAIEGELARTIETLEPIRSAVVHIAF
PKDSVFTERQIPPTASVVVNVREGLKLTRKQIDGIKNIVSAAVPKLTKENVKISDQSGVPLDEQEAYEDDLVRAQIKFKS
DQEKALEDKIIENLAPFAGGMDKVKVSVNIDFDFSKQESQSEIYDPNPIVRSEQTLNEERTGRKDPEIQGVPGAVSNIGP
VEGLDNKGQIDTYKKNQVTTNNELSKTITNTKKQFATVLRTSAAVTIDGKYQDVVDENGDVKSEYVPLTKEELASVESIV
KNTINFSATRGDSVVVQNLPFHRESIRVESKVKTFYNRFVEPFIPPVKYFIAAILLFIFYKKVIAPFTQKMLEDVAAQEE
MQQGPNAVLDDAEDALEKFNAARKKVEEQLGFGDNFNEDSIQYEVLLEKLRGLVSDKGEEIAALLQNLIQNDSEFGEKDM

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=560, Percent_Identity=30.8928571428571, Blast_Score=221, Evalue=1e-58,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 62590; Mature: 62590

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKNMLHQIGQLYQNLTRKQRIVIAASIVVVVGFLVFLALFRGSGSNANNGYAVLVENV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
SPSSSAAIVAKLEQNNIPYKLESENKILVPQDQVYRQRMFIASEGLIKDSRVGFEAFDTQ
CCCCCCEEEEEEECCCCCEEECCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCC
AFGATNEEQKVKYQRAIEGELARTIETLEPIRSAVVHIAFPKDSVFTERQIPPTASVVVN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHE
VREGLKLTRKQIDGIKNIVSAAVPKLTKENVKISDQSGVPLDEQEAYEDDLVRAQIKFKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHEEECC
DQEKALEDKIIENLAPFAGGMDKVKVSVNIDFDFSKQESQSEIYDPNPIVRSEQTLNEER
CHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
TGRKDPEIQGVPGAVSNIGPVEGLDNKGQIDTYKKNQVTTNNELSKTITNTKKQFATVLR
CCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TSAAVTIDGKYQDVVDENGDVKSEYVPLTKEELASVESIVKNTINFSATRGDSVVVQNLP
HCCEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
FHRESIRVESKVKTFYNRFVEPFIPPVKYFIAAILLFIFYKKVIAPFTQKMLEDVAAQEE
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQQGPNAVLDDAEDALEKFNAARKKVEEQLGFGDNFNEDSIQYEVLLEKLRGLVSDKGEE
HHCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
IAALLQNLIQNDSEFGEKDM
HHHHHHHHHHCCHHCCCCCC
>Mature Secondary Structure
MDFKNMLHQIGQLYQNLTRKQRIVIAASIVVVVGFLVFLALFRGSGSNANNGYAVLVENV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
SPSSSAAIVAKLEQNNIPYKLESENKILVPQDQVYRQRMFIASEGLIKDSRVGFEAFDTQ
CCCCCCEEEEEEECCCCCEEECCCCEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCC
AFGATNEEQKVKYQRAIEGELARTIETLEPIRSAVVHIAFPKDSVFTERQIPPTASVVVN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHE
VREGLKLTRKQIDGIKNIVSAAVPKLTKENVKISDQSGVPLDEQEAYEDDLVRAQIKFKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHEEECC
DQEKALEDKIIENLAPFAGGMDKVKVSVNIDFDFSKQESQSEIYDPNPIVRSEQTLNEER
CHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
TGRKDPEIQGVPGAVSNIGPVEGLDNKGQIDTYKKNQVTTNNELSKTITNTKKQFATVLR
CCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TSAAVTIDGKYQDVVDENGDVKSEYVPLTKEELASVESIVKNTINFSATRGDSVVVQNLP
HCCEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
FHRESIRVESKVKTFYNRFVEPFIPPVKYFIAAILLFIFYKKVIAPFTQKMLEDVAAQEE
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQQGPNAVLDDAEDALEKFNAARKKVEEQLGFGDNFNEDSIQYEVLLEKLRGLVSDKGEE
HHCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHH
IAALLQNLIQNDSEFGEKDM
HHHHHHHHHHCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2544561; 11677609; 1551848 [H]