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The map label for this gene is iscS [H]

Identifier: 57237300

GI number: 57237300

Start: 258158

End: 259339

Strand: Reverse

Name: iscS [H]

Synonym: CJE0291

Alternate gene names: 57237300

Gene position: 259339-258158 (Counterclockwise)

Preceding gene: 57237301

Following gene: 57237299

Centisome position: 14.59

GC content: 36.89

Gene sequence:

>1182_bases
GTGAAAGTATATTTAGATAACAACGCAACGACAATGCTTGATCCTAATGCTTATGAGTTGATGCTACCTTTTTTAAAGGA
TATATATGGAAATCCAAATAGCCTTCATCAATATGGAAGCGCAACACATCCTGCTTTAAGAGAAGCTTTAGATAAACTTT
ATGCAGGGCTTGGAGCAAATGACTTAGATGACATAGTTATAACCTCCTGTGCGACAGAAAGCATTAACTGGGTGCTTAAG
GGTGTGTATTTTGATCATATTTTAGATAGAGAGCGCAATGAAGTGATTATTTCTAGTGTAGAACACCCTGCTGTTGCTGC
TGCTGCTTATTTTTTAAAAAGTCTTGGAGTAAAAGTCATAGAACTTCCTGTAAACGAAGAAGGAGTTTCTACTGTAGAAG
ATTTGCGTAAAGTGATTAGCGATAAAACAGCACTTGTAAGTGTTATGTGGGCAAATAATGAAACAGGTATGATTTTTGAT
ATTAAAGCGATGGCAGAACTTGCGCATGAATTTGGTGCTCTTTTTCATACAGATGCTACTCAAGCAGTAGGTAAAATAAA
GGTTAATTTAACTCAAGTAGGAGTTGATTTTGCTTCATTTTCAGCTCATAAATTTCATGGACCAAAGGGTGTGGGTGGAC
TTTTTATAAAAAAAGGACTTAAGCTTACTCCACTTTTACACGGTGGAGAACACATGGGTGGTCGTAGAAGCGGGACTTTA
AATGTGCCTTATATAGTGGCTATGGGCGAGGCTTTGCGTATTGCAAATACTATGCTTGATTTTGAAGATTCTCATATACG
CCGTTTAAGAGATAAACTAGAAGATCAAATTTTAGCCTTGCCTGATACAACAGTGGTTGGAAAAAGAGAACACCGTGTGC
CTAATACAATCTTAGCAAGTATTAAAGGAGTTGAAGGCGAAGCAATGCTTTGGGATTTAAATAAAAACGGTATAGCTGCA
AGTACGGGCTCAGCCTGTGCAAGTGAAGCTTTAGAAAGCAATCCTATTATGGAAGCTATCGGTGCGGAGCATGATTTAGC
GCATACAGCTTTAAGGCTTTCTTTGTCACGTTTTAACACTGAAGAAGAAATTGATTATGCAGCAAAACAAATTAAAAATG
CAACACAGAGATTAAGAGCAATTTCTTGTACTTATGCTTATAATCCAAATAATTACAAATAA

Upstream 100 bases:

>100_bases
AATTTTTAACCTAATTTTTATAAATTTTTGTTATTATCCGTTTTTAAATAAAGATAAAAAATATCCTAATTAAAATTAAG
GATAAATTAAGGATTTTAAA

Downstream 100 bases:

>100_bases
GGATGAAAAATGGGAAAAAATAGTTTAATTGGTGGATCCATTTGGGATGAGTATTCACAAAAAGTTCAAGATAGAATGAA
TAATCCTCAACATATGGGAG

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKVYLDNNATTMLDPNAYELMLPFLKDIYGNPNSLHQYGSATHPALREALDKLYAGLGANDLDDIVITSCATESINWVLK
GVYFDHILDRERNEVIISSVEHPAVAAAAYFLKSLGVKVIELPVNEEGVSTVEDLRKVISDKTALVSVMWANNETGMIFD
IKAMAELAHEFGALFHTDATQAVGKIKVNLTQVGVDFASFSAHKFHGPKGVGGLFIKKGLKLTPLLHGGEHMGGRRSGTL
NVPYIVAMGEALRIANTMLDFEDSHIRRLRDKLEDQILALPDTTVVGKREHRVPNTILASIKGVEGEAMLWDLNKNGIAA
STGSACASEALESNPIMEAIGAEHDLAHTALRLSLSRFNTEEEIDYAAKQIKNATQRLRAISCTYAYNPNNYK

Sequences:

>Translated_393_residues
MKVYLDNNATTMLDPNAYELMLPFLKDIYGNPNSLHQYGSATHPALREALDKLYAGLGANDLDDIVITSCATESINWVLK
GVYFDHILDRERNEVIISSVEHPAVAAAAYFLKSLGVKVIELPVNEEGVSTVEDLRKVISDKTALVSVMWANNETGMIFD
IKAMAELAHEFGALFHTDATQAVGKIKVNLTQVGVDFASFSAHKFHGPKGVGGLFIKKGLKLTPLLHGGEHMGGRRSGTL
NVPYIVAMGEALRIANTMLDFEDSHIRRLRDKLEDQILALPDTTVVGKREHRVPNTILASIKGVEGEAMLWDLNKNGIAA
STGSACASEALESNPIMEAIGAEHDLAHTALRLSLSRFNTEEEIDYAAKQIKNATQRLRAISCTYAYNPNNYK
>Mature_393_residues
MKVYLDNNATTMLDPNAYELMLPFLKDIYGNPNSLHQYGSATHPALREALDKLYAGLGANDLDDIVITSCATESINWVLK
GVYFDHILDRERNEVIISSVEHPAVAAAAYFLKSLGVKVIELPVNEEGVSTVEDLRKVISDKTALVSVMWANNETGMIFD
IKAMAELAHEFGALFHTDATQAVGKIKVNLTQVGVDFASFSAHKFHGPKGVGGLFIKKGLKLTPLLHGGEHMGGRRSGTL
NVPYIVAMGEALRIANTMLDFEDSHIRRLRDKLEDQILALPDTTVVGKREHRVPNTILASIKGVEGEAMLWDLNKNGIAA
STGSACASEALESNPIMEAIGAEHDLAHTALRLSLSRFNTEEEIDYAAKQIKNATQRLRAISCTYAYNPNNYK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=384, Percent_Identity=37.2395833333333, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI156713448, Length=423, Percent_Identity=32.1513002364066, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI48994898, Length=387, Percent_Identity=38.7596899224806, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1789175, Length=400, Percent_Identity=26.75, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787970, Length=336, Percent_Identity=27.6785714285714, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25143064, Length=384, Percent_Identity=37.5, Blast_Score=251, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI17533177, Length=321, Percent_Identity=29.9065420560748, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319831, Length=383, Percent_Identity=39.1644908616188, Blast_Score=265, Evalue=8e-72,
Organism=Drosophila melanogaster, GI20129463, Length=384, Percent_Identity=36.9791666666667, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017773
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43048; Mature: 43048

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00595 AA_TRANSFER_CLASS_5 ; PS00132 CARBOXYPEPT_ZN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYLDNNATTMLDPNAYELMLPFLKDIYGNPNSLHQYGSATHPALREALDKLYAGLGAN
CEEEECCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCC
DLDDIVITSCATESINWVLKGVYFDHILDRERNEVIISSVEHPAVAAAAYFLKSLGVKVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEE
ELPVNEEGVSTVEDLRKVISDKTALVSVMWANNETGMIFDIKAMAELAHEFGALFHTDAT
EECCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCHH
QAVGKIKVNLTQVGVDFASFSAHKFHGPKGVGGLFIKKGLKLTPLLHGGEHMGGRRSGTL
HHHEEEEEEEEEECCCHHHCCCHHCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCC
NVPYIVAMGEALRIANTMLDFEDSHIRRLRDKLEDQILALPDTTVVGKREHRVPNTILAS
CCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCEEECCCCEEECCHHCCCCHHHHHH
IKGVEGEAMLWDLNKNGIAASTGSACASEALESNPIMEAIGAEHDLAHTALRLSLSRFNT
HCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCC
EEEIDYAAKQIKNATQRLRAISCTYAYNPNNYK
HHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCC
>Mature Secondary Structure
MKVYLDNNATTMLDPNAYELMLPFLKDIYGNPNSLHQYGSATHPALREALDKLYAGLGAN
CEEEECCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCC
DLDDIVITSCATESINWVLKGVYFDHILDRERNEVIISSVEHPAVAAAAYFLKSLGVKVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEE
ELPVNEEGVSTVEDLRKVISDKTALVSVMWANNETGMIFDIKAMAELAHEFGALFHTDAT
EECCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCHH
QAVGKIKVNLTQVGVDFASFSAHKFHGPKGVGGLFIKKGLKLTPLLHGGEHMGGRRSGTL
HHHEEEEEEEEEECCCHHHCCCHHCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCC
NVPYIVAMGEALRIANTMLDFEDSHIRRLRDKLEDQILALPDTTVVGKREHRVPNTILAS
CCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCEEECCCCEEECCHHCCCCHHHHHH
IKGVEGEAMLWDLNKNGIAASTGSACASEALESNPIMEAIGAEHDLAHTALRLSLSRFNT
HCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCC
EEEIDYAAKQIKNATQRLRAISCTYAYNPNNYK
HHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA