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The map label for this gene is nifU [H]

Identifier: 57237299

GI number: 57237299

Start: 257177

End: 258148

Strand: Reverse

Name: nifU [H]

Synonym: CJE0290

Alternate gene names: 57237299

Gene position: 258148-257177 (Counterclockwise)

Preceding gene: 57237300

Following gene: 57237296

Centisome position: 14.52

GC content: 35.19

Gene sequence:

>972_bases
ATGGGAAAAAATAGTTTAATTGGTGGATCCATTTGGGATGAGTATTCACAAAAAGTTCAAGATAGAATGAATAATCCTCA
ACATATGGGAGAATTTAGTGAAGAAGATGCTAAAGCACGCAATGCAAAATTGATCGTAGCTGATTTTGGTGCAGAAAGTT
GCGGCGATGCGGTAAGACTTTTTTGGCTTGTAGATGAAAAAACTGATAAAATTATCGATGCAAAATTTAAAAGTTTTGGT
TGTGGTACAGCTATAGCAAGTAGTGATACTATGGTGGATCTTTGTATAGGAAAAACTGTAGATGAAGCAGTAAAAATCAC
AAATTTAGATGTGGAATTTGCTATGCGTGATAATCCAGAAACTCCAGCTGTTCCACCTCAAAAAATGCACTGTTCGGTTA
TGGCTTATGATGTAATCAAACAAGCAGCAGCGCATTATAAAGGTATAAGTCCTGAAGATTTTGAAGATCAAATTATAGTT
TGCGAGTGCGCTAGGGTAAGTCTTGGAACTATTAAAGAAGTGATTAAACTTAATGATTTGCATAGCGTAGAAGAAATCAC
ACAATATACTAAAGCGGGTGCTTTTTGTAAATCCTGTATCAAACCAGGCGGACATGAAAAAAGAGATTATTACCTTGTAG
ATATTTTAGCTGAAACTAGAGCTGAAATAGATAGAGAAAAACTCAAAAATACAATGAAAAGTGATGTAGCTTTTGATGAA
ATGACCGTAGTGGGACAATTAAAAGCAGTTGAAAGTGTTTTAGATGCTGAAATTCGCCCTATGCTTCATAATGATGGTGG
AGATTTGGAAGTGATTGATATACAAAAGGCTGAAGGTGCAGCAATTGATGTTTATATTCGTTATCTTGGTGCTTGTAGTG
GTTGTTCTAGTGGAAGTGGTGCAACACTTTATGCTATTGAAACAATTTTACAAGAAGAATTAAGTCCAAATATACGCGTT
ATGCCGGTTTAA

Upstream 100 bases:

>100_bases
TGATTATGCAGCAAAACAAATTAAAAATGCAACACAGAGATTAAGAGCAATTTCTTGTACTTATGCTTATAATCCAAATA
ATTACAAATAAGGATGAAAA

Downstream 100 bases:

>100_bases
AAAATAGCAAGATAAAATCTTGCTATTTTATAGCTTTTGCTTGCAAATCAAGGGGTGGTAAATTTTCTATATAAATACTA
CGACTTGGGAAAGCAAAGGT

Product: NifU family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MGKNSLIGGSIWDEYSQKVQDRMNNPQHMGEFSEEDAKARNAKLIVADFGAESCGDAVRLFWLVDEKTDKIIDAKFKSFG
CGTAIASSDTMVDLCIGKTVDEAVKITNLDVEFAMRDNPETPAVPPQKMHCSVMAYDVIKQAAAHYKGISPEDFEDQIIV
CECARVSLGTIKEVIKLNDLHSVEEITQYTKAGAFCKSCIKPGGHEKRDYYLVDILAETRAEIDREKLKNTMKSDVAFDE
MTVVGQLKAVESVLDAEIRPMLHNDGGDLEVIDIQKAEGAAIDVYIRYLGACSGCSSGSGATLYAIETILQEELSPNIRV
MPV

Sequences:

>Translated_323_residues
MGKNSLIGGSIWDEYSQKVQDRMNNPQHMGEFSEEDAKARNAKLIVADFGAESCGDAVRLFWLVDEKTDKIIDAKFKSFG
CGTAIASSDTMVDLCIGKTVDEAVKITNLDVEFAMRDNPETPAVPPQKMHCSVMAYDVIKQAAAHYKGISPEDFEDQIIV
CECARVSLGTIKEVIKLNDLHSVEEITQYTKAGAFCKSCIKPGGHEKRDYYLVDILAETRAEIDREKLKNTMKSDVAFDE
MTVVGQLKAVESVLDAEIRPMLHNDGGDLEVIDIQKAEGAAIDVYIRYLGACSGCSSGSGATLYAIETILQEELSPNIRV
MPV
>Mature_322_residues
GKNSLIGGSIWDEYSQKVQDRMNNPQHMGEFSEEDAKARNAKLIVADFGAESCGDAVRLFWLVDEKTDKIIDAKFKSFGC
GTAIASSDTMVDLCIGKTVDEAVKITNLDVEFAMRDNPETPAVPPQKMHCSVMAYDVIKQAAAHYKGISPEDFEDQIIVC
ECARVSLGTIKEVIKLNDLHSVEEITQYTKAGAFCKSCIKPGGHEKRDYYLVDILAETRAEIDREKLKNTMKSDVAFDEM
TVVGQLKAVESVLDAEIRPMLHNDGGDLEVIDIQKAEGAAIDVYIRYLGACSGCSSGSGATLYAIETILQEELSPNIRVM
PV

Specific function: Involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=133, Percent_Identity=42.8571428571429, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI24307953, Length=134, Percent_Identity=41.7910447761194, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788878, Length=133, Percent_Identity=39.8496240601504, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17543474, Length=134, Percent_Identity=39.5522388059701, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324800, Length=133, Percent_Identity=39.0977443609023, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325122, Length=133, Percent_Identity=39.8496240601504, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355597, Length=133, Percent_Identity=40.6015037593985, Blast_Score=98, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR016217
- InterPro:   IPR010238
- InterPro:   IPR001075
- InterPro:   IPR002871
- ProDom:   PD002830 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01106 NifU; PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 35491; Mature: 35360

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNSLIGGSIWDEYSQKVQDRMNNPQHMGEFSEEDAKARNAKLIVADFGAESCGDAVRL
CCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHCCCEEEEEECCCCCCCCEEEE
FWLVDEKTDKIIDAKFKSFGCGTAIASSDTMVDLCIGKTVDEAVKITNLDVEFAMRDNPE
EEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEHHCCCHHHHHEEECCEEEEEECCCCC
TPAVPPQKMHCSVMAYDVIKQAAAHYKGISPEDFEDQIIVCECARVSLGTIKEVIKLNDL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCH
HSVEEITQYTKAGAFCKSCIKPGGHEKRDYYLVDILAETRAEIDREKLKNTMKSDVAFDE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHH
MTVVGQLKAVESVLDAEIRPMLHNDGGDLEVIDIQKAEGAAIDVYIRYLGACSGCSSGSG
HHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCC
ATLYAIETILQEELSPNIRVMPV
CCHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
GKNSLIGGSIWDEYSQKVQDRMNNPQHMGEFSEEDAKARNAKLIVADFGAESCGDAVRL
CCCCCCCCHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHCCCEEEEEECCCCCCCCEEEE
FWLVDEKTDKIIDAKFKSFGCGTAIASSDTMVDLCIGKTVDEAVKITNLDVEFAMRDNPE
EEEEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEHHCCCHHHHHEEECCEEEEEECCCCC
TPAVPPQKMHCSVMAYDVIKQAAAHYKGISPEDFEDQIIVCECARVSLGTIKEVIKLNDL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCH
HSVEEITQYTKAGAFCKSCIKPGGHEKRDYYLVDILAETRAEIDREKLKNTMKSDVAFDE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHH
MTVVGQLKAVESVLDAEIRPMLHNDGGDLEVIDIQKAEGAAIDVYIRYLGACSGCSSGSG
HHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCC
ATLYAIETILQEELSPNIRVMPV
CCHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672; 7947754; 10819462 [H]