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The map label for this gene is thrS [H]

Identifier: 57237210

GI number: 57237210

Start: 200275

End: 202083

Strand: Direct

Name: thrS [H]

Synonym: CJE0199

Alternate gene names: 57237210

Gene position: 200275-202083 (Clockwise)

Preceding gene: 57237209

Following gene: 57237211

Centisome position: 11.27

GC content: 33.94

Gene sequence:

>1809_bases
ATGGAAAAAGAAGTTATTGCGTATTTGGACAATGAGACGATAATTGACTCTCAAAGTGTGAAAAATACAAATTTAAAAGA
AATTTATTTTGATAATTCTAAAGAAAGTTTAGAAGTGATACGCCATTCTTGTGCGCATTTGATGGCACAGGCGATTAAGA
GCTTGTATCCTGAGGCTAAATTTTTCGTTGGTCCTGTTATAGAAGATGGATTTTATTATGATTTTCGTGTAGAAAGTAAG
ATTGGTGAAGAAGATTTAGTAAAAATTGAAAAAAAGATGAAAGAACTTGCTGAAGCAAAGATTGAAATTTCAAAATATGA
AATCACAAAAAACGAAGCTTTGGCTAAATTTCAAAATGATGATTTAAAACAAGAAGTTTTATTGCGTATTCCTGATGGAG
CTGTAAGTATTTATAAACAAGGTGAATTTGAAGATTTGTGTCGTGGTCCTCATGTGCCAAATACGAAATTTTTACGCTTT
TTTAAACTCACTCGTGTTGCAGGAGCGTATTTGGATGGCGATGAAAAAAGAGAAATGCTTACTCGAATTTATGGGACTGC
TTTTGCAGATAAGGAAAGTTTAAAAGAGTATTTAACCATTATTGAAGAAGCTAAAAAACGCGATCATAGAAAGCTTGGAA
CAGAGCTTAAACTTTTTACTTTTGATGATGAGATCGGAGGAGGCCTTCCTATCTGGTTAAGTAATGGTGCAAGACTTAGA
TCTAAACTTGAACATATGCTTTATAAAATTCATCGTTTGCGTGGTTATGAGCCTGTGCGTGGGCCTGAGCTTTTAAAAGC
AGATGCATGGAAAATCAGTGGGCATTATGCAAACTATAAAGAAAATATGTATTTTACACAAATTGATGAACAAGAGTATG
GTATTAAGCCTATGAACTGTGTGGGGCATATTAAAATTTATCAAAGTGATGTAAGAAGCTATCGTGATTTGCCTTTGAAA
TTTTTTGAATACGGCGTAGTGCATCGTCATGAAAAAAGTGGGGTTTTGCACGGACTTTTTAGAGTGAGAGAATTCACTCA
AGATGATGCACATATTTTTTGTATGCCAAGTCAGATTAAAGAGCAGGTTTTAGAAATTTTAGCTTTTGTGGATAATTTGA
TGAAATTATTTGATTTTAGCTATGAAATGGAAATTTCAACTAAACCTGAAAAAGCCATAGGTGATGATGAAATTTGGGAA
GTGGCGACTAAGGCTTTAAAAGAAGCTTTAGATGAACAAGGTTTAAAATACGGCATCGATGAAGGTGGCGGGGCTTTTTA
TGGGCCAAAGATTGATATTAAAATCACTGATGCTTTAAAAAGAAAGTGGCAATGTGGAACTATACAAGTAGATTTTAACC
TGCCAAGTCGTTTTAAACTTGAATACACTGATAGTGATAATGAAAAAAAACAACCTGTAATGTTGCATCGTGCGATTTTA
GGTTCTTTTGAAAGATTTATAGGAATTTTAACCGAGCATTGTGCGGGTGAGTTTCCATTTTTTATCGCACCAACTGCGGT
GGGTATAGTGCCAATAGGCGAAGCACATATTGCTTACGCAAAAGAAATTCAAAAAGAATTATTAGAGCTTAATATCGATA
GCGAGGTGTATGAAAAAAATGAGAGCTTAAGTAAAAAAATTCGTATCGCTGAAAAGCAAAAATTACCTATGATTTTAGTT
TTAGGCGATGATGAGGTGGCAAAGCGTAGCGTTGCTTTAAGAGATAGAAGAGCTAAAGAACAAAAAAATCTTAGCTTGGA
CGAATTTATTAAATTAGTTAAGGAGAAAATGAGTGAGGTACACTTTTGA

Upstream 100 bases:

>100_bases
TAAAAATAAGCTGAATTTCAAAAATAATTAAGTCTTAGAAAGTTAAAATTAGAACTTTATAAACACTCACAGGGTAAGTG
AGCGACAAAGGGTTAAAAAT

Downstream 100 bases:

>100_bases
GTAAAGAAAAAGAAGTATTGCTCAATGAAGAAATAAGAGCGGATGAAATCAGATGTGTTGGCGATGATGGCAAAGTTTAT
GGTATTATCAGTAGTGATGA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MEKEVIAYLDNETIIDSQSVKNTNLKEIYFDNSKESLEVIRHSCAHLMAQAIKSLYPEAKFFVGPVIEDGFYYDFRVESK
IGEEDLVKIEKKMKELAEAKIEISKYEITKNEALAKFQNDDLKQEVLLRIPDGAVSIYKQGEFEDLCRGPHVPNTKFLRF
FKLTRVAGAYLDGDEKREMLTRIYGTAFADKESLKEYLTIIEEAKKRDHRKLGTELKLFTFDDEIGGGLPIWLSNGARLR
SKLEHMLYKIHRLRGYEPVRGPELLKADAWKISGHYANYKENMYFTQIDEQEYGIKPMNCVGHIKIYQSDVRSYRDLPLK
FFEYGVVHRHEKSGVLHGLFRVREFTQDDAHIFCMPSQIKEQVLEILAFVDNLMKLFDFSYEMEISTKPEKAIGDDEIWE
VATKALKEALDEQGLKYGIDEGGGAFYGPKIDIKITDALKRKWQCGTIQVDFNLPSRFKLEYTDSDNEKKQPVMLHRAIL
GSFERFIGILTEHCAGEFPFFIAPTAVGIVPIGEAHIAYAKEIQKELLELNIDSEVYEKNESLSKKIRIAEKQKLPMILV
LGDDEVAKRSVALRDRRAKEQKNLSLDEFIKLVKEKMSEVHF

Sequences:

>Translated_602_residues
MEKEVIAYLDNETIIDSQSVKNTNLKEIYFDNSKESLEVIRHSCAHLMAQAIKSLYPEAKFFVGPVIEDGFYYDFRVESK
IGEEDLVKIEKKMKELAEAKIEISKYEITKNEALAKFQNDDLKQEVLLRIPDGAVSIYKQGEFEDLCRGPHVPNTKFLRF
FKLTRVAGAYLDGDEKREMLTRIYGTAFADKESLKEYLTIIEEAKKRDHRKLGTELKLFTFDDEIGGGLPIWLSNGARLR
SKLEHMLYKIHRLRGYEPVRGPELLKADAWKISGHYANYKENMYFTQIDEQEYGIKPMNCVGHIKIYQSDVRSYRDLPLK
FFEYGVVHRHEKSGVLHGLFRVREFTQDDAHIFCMPSQIKEQVLEILAFVDNLMKLFDFSYEMEISTKPEKAIGDDEIWE
VATKALKEALDEQGLKYGIDEGGGAFYGPKIDIKITDALKRKWQCGTIQVDFNLPSRFKLEYTDSDNEKKQPVMLHRAIL
GSFERFIGILTEHCAGEFPFFIAPTAVGIVPIGEAHIAYAKEIQKELLELNIDSEVYEKNESLSKKIRIAEKQKLPMILV
LGDDEVAKRSVALRDRRAKEQKNLSLDEFIKLVKEKMSEVHF
>Mature_602_residues
MEKEVIAYLDNETIIDSQSVKNTNLKEIYFDNSKESLEVIRHSCAHLMAQAIKSLYPEAKFFVGPVIEDGFYYDFRVESK
IGEEDLVKIEKKMKELAEAKIEISKYEITKNEALAKFQNDDLKQEVLLRIPDGAVSIYKQGEFEDLCRGPHVPNTKFLRF
FKLTRVAGAYLDGDEKREMLTRIYGTAFADKESLKEYLTIIEEAKKRDHRKLGTELKLFTFDDEIGGGLPIWLSNGARLR
SKLEHMLYKIHRLRGYEPVRGPELLKADAWKISGHYANYKENMYFTQIDEQEYGIKPMNCVGHIKIYQSDVRSYRDLPLK
FFEYGVVHRHEKSGVLHGLFRVREFTQDDAHIFCMPSQIKEQVLEILAFVDNLMKLFDFSYEMEISTKPEKAIGDDEIWE
VATKALKEALDEQGLKYGIDEGGGAFYGPKIDIKITDALKRKWQCGTIQVDFNLPSRFKLEYTDSDNEKKQPVMLHRAIL
GSFERFIGILTEHCAGEFPFFIAPTAVGIVPIGEAHIAYAKEIQKELLELNIDSEVYEKNESLSKKIRIAEKQKLPMILV
LGDDEVAKRSVALRDRRAKEQKNLSLDEFIKLVKEKMSEVHF

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=596, Percent_Identity=37.751677852349, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI38570111, Length=583, Percent_Identity=37.7358490566038, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI20070344, Length=595, Percent_Identity=33.4453781512605, Blast_Score=351, Evalue=1e-96,
Organism=Escherichia coli, GI1788013, Length=603, Percent_Identity=42.7860696517413, Blast_Score=504, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71984184, Length=595, Percent_Identity=38.3193277310924, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71984192, Length=595, Percent_Identity=38.3193277310924, Blast_Score=428, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322112, Length=582, Percent_Identity=39.0034364261168, Blast_Score=445, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322654, Length=387, Percent_Identity=36.4341085271318, Blast_Score=271, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24583839, Length=572, Percent_Identity=41.0839160839161, Blast_Score=450, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24583842, Length=572, Percent_Identity=41.0839160839161, Blast_Score=450, Evalue=1e-126,
Organism=Drosophila melanogaster, GI28574141, Length=572, Percent_Identity=41.0839160839161, Blast_Score=450, Evalue=1e-126,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 69587; Mature: 69587

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEVIAYLDNETIIDSQSVKNTNLKEIYFDNSKESLEVIRHSCAHLMAQAIKSLYPEAK
CCCCEEEEECCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
FFVGPVIEDGFYYDFRVESKIGEEDLVKIEKKMKELAEAKIEISKYEITKNEALAKFQND
EEECCEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEHHEEEECHHHHHHHHCCC
DLKQEVLLRIPDGAVSIYKQGEFEDLCRGPHVPNTKFLRFFKLTRVAGAYLDGDEKREML
CHHHHHHEECCCCCEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
TRIYGTAFADKESLKEYLTIIEEAKKRDHRKLGTELKLFTFDDEIGGGLPIWLSNGARLR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCCHHHH
SKLEHMLYKIHRLRGYEPVRGPELLKADAWKISGHYANYKENMYFTQIDEQEYGIKPMNC
HHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEECCCCCCCCCCEEEEEECCHHCCCCCCCH
VGHIKIYQSDVRSYRDLPLKFFEYGVVHRHEKSGVLHGLFRVREFTQDDAHIFCMPSQIK
HEEEEEEHHHHHHHHCCCHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
EQVLEILAFVDNLMKLFDFSYEMEISTKPEKAIGDDEIWEVATKALKEALDEQGLKYGID
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCC
EGGGAFYGPKIDIKITDALKRKWQCGTIQVDFNLPSRFKLEYTDSDNEKKQPVMLHRAIL
CCCCEEECCEEEEEEEHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHH
GSFERFIGILTEHCAGEFPFFIAPTAVGIVPIGEAHIAYAKEIQKELLELNIDSEVYEKN
HHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCC
ESLSKKIRIAEKQKLPMILVLGDDEVAKRSVALRDRRAKEQKNLSLDEFIKLVKEKMSEV
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
HF
CC
>Mature Secondary Structure
MEKEVIAYLDNETIIDSQSVKNTNLKEIYFDNSKESLEVIRHSCAHLMAQAIKSLYPEAK
CCCCEEEEECCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
FFVGPVIEDGFYYDFRVESKIGEEDLVKIEKKMKELAEAKIEISKYEITKNEALAKFQND
EEECCEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEHHEEEECHHHHHHHHCCC
DLKQEVLLRIPDGAVSIYKQGEFEDLCRGPHVPNTKFLRFFKLTRVAGAYLDGDEKREML
CHHHHHHEECCCCCEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
TRIYGTAFADKESLKEYLTIIEEAKKRDHRKLGTELKLFTFDDEIGGGLPIWLSNGARLR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCCHHHH
SKLEHMLYKIHRLRGYEPVRGPELLKADAWKISGHYANYKENMYFTQIDEQEYGIKPMNC
HHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEECCCCCCCCCCEEEEEECCHHCCCCCCCH
VGHIKIYQSDVRSYRDLPLKFFEYGVVHRHEKSGVLHGLFRVREFTQDDAHIFCMPSQIK
HEEEEEEHHHHHHHHCCCHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
EQVLEILAFVDNLMKLFDFSYEMEISTKPEKAIGDDEIWEVATKALKEALDEQGLKYGID
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCC
EGGGAFYGPKIDIKITDALKRKWQCGTIQVDFNLPSRFKLEYTDSDNEKKQPVMLHRAIL
CCCCEEECCEEEEEEEHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHH
GSFERFIGILTEHCAGEFPFFIAPTAVGIVPIGEAHIAYAKEIQKELLELNIDSEVYEKN
HHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCC
ESLSKKIRIAEKQKLPMILVLGDDEVAKRSVALRDRRAKEQKNLSLDEFIKLVKEKMSEV
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
HF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA