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The map label for this gene is purF [H]

Identifier: 57237201

GI number: 57237201

Start: 189585

End: 190922

Strand: Reverse

Name: purF [H]

Synonym: CJE0189

Alternate gene names: 57237201

Gene position: 190922-189585 (Counterclockwise)

Preceding gene: 57237202

Following gene: 57237198

Centisome position: 10.74

GC content: 34.45

Gene sequence:

>1338_bases
ATGTGTGCAGTTGTAGGAGTTATCAATTCAAAAAATGCTAGCACTTATGCGTATTATGCGCTTTTTGCTATGCAACACCG
TGGGCAAGAAGCAAGTGGCATTAGTGTGAGTAATGGTAAAAATATTAAGACTATTAAAGCTAAAGGTGAAGTGAGTCAAA
TTTTTAATCCTGATAATCTAAAAACTTTAGAAGGTGAAATCGCTATAGGGCATAATCGCTATTCTACAGCAGGAAATTCA
AGTTTAAATGACGCTCAGCCCATAGCAGCTACTTCGGTTTTAGGTGATATTGCTTTAGCGCATAATGGAAATTTAGTCAA
TAAAGAAGAGGTAAGATCAAGACTTATTCAAGATGGGGCTATTTTTCAAACCAATATGGATACAGAAAATGTTGTCCATC
TTATCGCAAGAAGTAAGCAAGAAAGTCTAAAAGATAGATTTATTGAGAGTTTAAAAGAATGTATAGGAGCTTATTGTTTT
GTTTTAGCAAGCAAAGATAAACTTTATGTTGTAAGAGATCCTCATGGGGTGCGTCCTTTGTCTTTAGGGCGTTTAAAAGA
TGGAGGCTATATAGTTGCTAGTGAAACTTGTGCTTTTGATCTTATAGAGGCTGAATTTATTCGTGATGTTAAACCAGGGG
AAATGCTTATTTTTACTCAGGGAAATGATAAATTTGAAAGCATAGAACTTTTTTCTCAAACTCCTAGAATTTGCGCTTTT
GAATACATTTATTTTGCTAGACCTGATAGTATAGTAGAAGGCAAAAGCGTGTATGAAGTGCGTAAAAAAATGGGCGAAGC
TTTAGCTAAAAAATTTGCTTATAAAGCAGACTTTGTCGTGCCTGTGCCAGATAGTGGAGTAAGTGCAGCTATAGGTTTTG
CGCAATATTTACAAATCCCTCTTGAAATGGCAATAGTAAGAAATCATTATGTAGGAAGAACTTTTATAGAGCCAACTCAA
GAGCTTAGAAATTTAAAAGTAAAATTAAAACTTAATCCTATGCGTAAAGTTTTAGAAGGCAAAGAAATCGTAGTCATTGA
TGATAGCTTGGTGCGTGGCACTACTTCTAAAAAAATAATTTCCTTACTTCGTGCTGCAGGAGCGAGTAAAATCCATCTTG
CCATAGCTTGTCCTGAGATCAAATTTCCTGATACTTATGGTATAGATACTCCAACTTTTGAAGAACTTATCAGCGCAAAT
AAAAATGCTGAAGAAGTGCGTGAATATGTAGAAGCTGATACGCTAAGTTTTTTAAGCATAAAAGAGCTAACTCAAAGCAT
AGGCGATGAAAGAAAATATTCTTTAATCAGTTTTGATGGAGATTATTTTATTAAATAA

Upstream 100 bases:

>100_bases
TCAAGGGCGACTTTTGCTAAAGGTGCGATTAAAATCGCTATTTGGCTTAGCAAGCAAGAAGCAAAAATGTATTCAATCAA
TGATTTTTTAGGAATTTAAA

Downstream 100 bases:

>100_bases
TTTTATTTTAAATTAGGATTAGGCAAGGTTGCACTTGCACTTCCTACATTTATATTTTGAGTTGGTGGCAAAGGAGTATT
TGGTGTATCAATTTGACTTA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MCAVVGVINSKNASTYAYYALFAMQHRGQEASGISVSNGKNIKTIKAKGEVSQIFNPDNLKTLEGEIAIGHNRYSTAGNS
SLNDAQPIAATSVLGDIALAHNGNLVNKEEVRSRLIQDGAIFQTNMDTENVVHLIARSKQESLKDRFIESLKECIGAYCF
VLASKDKLYVVRDPHGVRPLSLGRLKDGGYIVASETCAFDLIEAEFIRDVKPGEMLIFTQGNDKFESIELFSQTPRICAF
EYIYFARPDSIVEGKSVYEVRKKMGEALAKKFAYKADFVVPVPDSGVSAAIGFAQYLQIPLEMAIVRNHYVGRTFIEPTQ
ELRNLKVKLKLNPMRKVLEGKEIVVIDDSLVRGTTSKKIISLLRAAGASKIHLAIACPEIKFPDTYGIDTPTFEELISAN
KNAEEVREYVEADTLSFLSIKELTQSIGDERKYSLISFDGDYFIK

Sequences:

>Translated_445_residues
MCAVVGVINSKNASTYAYYALFAMQHRGQEASGISVSNGKNIKTIKAKGEVSQIFNPDNLKTLEGEIAIGHNRYSTAGNS
SLNDAQPIAATSVLGDIALAHNGNLVNKEEVRSRLIQDGAIFQTNMDTENVVHLIARSKQESLKDRFIESLKECIGAYCF
VLASKDKLYVVRDPHGVRPLSLGRLKDGGYIVASETCAFDLIEAEFIRDVKPGEMLIFTQGNDKFESIELFSQTPRICAF
EYIYFARPDSIVEGKSVYEVRKKMGEALAKKFAYKADFVVPVPDSGVSAAIGFAQYLQIPLEMAIVRNHYVGRTFIEPTQ
ELRNLKVKLKLNPMRKVLEGKEIVVIDDSLVRGTTSKKIISLLRAAGASKIHLAIACPEIKFPDTYGIDTPTFEELISAN
KNAEEVREYVEADTLSFLSIKELTQSIGDERKYSLISFDGDYFIK
>Mature_445_residues
MCAVVGVINSKNASTYAYYALFAMQHRGQEASGISVSNGKNIKTIKAKGEVSQIFNPDNLKTLEGEIAIGHNRYSTAGNS
SLNDAQPIAATSVLGDIALAHNGNLVNKEEVRSRLIQDGAIFQTNMDTENVVHLIARSKQESLKDRFIESLKECIGAYCF
VLASKDKLYVVRDPHGVRPLSLGRLKDGGYIVASETCAFDLIEAEFIRDVKPGEMLIFTQGNDKFESIELFSQTPRICAF
EYIYFARPDSIVEGKSVYEVRKKMGEALAKKFAYKADFVVPVPDSGVSAAIGFAQYLQIPLEMAIVRNHYVGRTFIEPTQ
ELRNLKVKLKLNPMRKVLEGKEIVVIDDSLVRGTTSKKIISLLRAAGASKIHLAIACPEIKFPDTYGIDTPTFEELISAN
KNAEEVREYVEADTLSFLSIKELTQSIGDERKYSLISFDGDYFIK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=460, Percent_Identity=36.9565217391304, Blast_Score=306, Evalue=2e-83,
Organism=Escherichia coli, GI1788651, Length=471, Percent_Identity=37.5796178343949, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI1790167, Length=213, Percent_Identity=26.2910798122066, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=459, Percent_Identity=37.4727668845316, Blast_Score=306, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6323958, Length=473, Percent_Identity=34.8837209302326, Blast_Score=259, Evalue=5e-70,
Organism=Drosophila melanogaster, GI28573187, Length=457, Percent_Identity=37.855579868709, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24659598, Length=433, Percent_Identity=37.6443418013857, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24659604, Length=433, Percent_Identity=37.6443418013857, Blast_Score=305, Evalue=4e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 49340; Mature: 49340

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAVVGVINSKNASTYAYYALFAMQHRGQEASGISVSNGKNIKTIKAKGEVSQIFNPDNL
CEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEECCCHHHCCCCCCC
KTLEGEIAIGHNRYSTAGNSSLNDAQPIAATSVLGDIALAHNGNLVNKEEVRSRLIQDGA
EEECCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEEEECCCCCCCHHHHHHHHHCCCC
IFQTNMDTENVVHLIARSKQESLKDRFIESLKECIGAYCFVLASKDKLYVVRDPHGVRPL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCCCCCC
SLGRLKDGGYIVASETCAFDLIEAEFIRDVKPGEMLIFTQGNDKFESIELFSQTPRICAF
CCCEECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCCCEEEE
EYIYFARPDSIVEGKSVYEVRKKMGEALAKKFAYKADFVVPVPDSGVSAAIGFAQYLQIP
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHH
LEMAIVRNHYVGRTFIEPTQELRNLKVKLKLNPMRKVLEGKEIVVIDDSLVRGTTSKKII
HHHHHHHHCCCCCHHCCHHHHHCCEEEEEEECHHHHHHCCCEEEEECCHHHCCCCHHHHH
SLLRAAGASKIHLAIACPEIKFPDTYGIDTPTFEELISANKNAEEVREYVEADTLSFLSI
HHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KELTQSIGDERKYSLISFDGDYFIK
HHHHHHHCCCCCEEEEEECCCEEEC
>Mature Secondary Structure
MCAVVGVINSKNASTYAYYALFAMQHRGQEASGISVSNGKNIKTIKAKGEVSQIFNPDNL
CEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEECCCHHHCCCCCCC
KTLEGEIAIGHNRYSTAGNSSLNDAQPIAATSVLGDIALAHNGNLVNKEEVRSRLIQDGA
EEECCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEEEECCCCCCCHHHHHHHHHCCCC
IFQTNMDTENVVHLIARSKQESLKDRFIESLKECIGAYCFVLASKDKLYVVRDPHGVRPL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCCCCCC
SLGRLKDGGYIVASETCAFDLIEAEFIRDVKPGEMLIFTQGNDKFESIELFSQTPRICAF
CCCEECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCCCEEEE
EYIYFARPDSIVEGKSVYEVRKKMGEALAKKFAYKADFVVPVPDSGVSAAIGFAQYLQIP
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHH
LEMAIVRNHYVGRTFIEPTQELRNLKVKLKLNPMRKVLEGKEIVVIDDSLVRGTTSKKII
HHHHHHHHCCCCCHHCCHHHHHCCEEEEEEECHHHHHHCCCEEEEECCHHHCCCCHHHHH
SLLRAAGASKIHLAIACPEIKFPDTYGIDTPTFEELISANKNAEEVREYVEADTLSFLSI
HHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KELTQSIGDERKYSLISFDGDYFIK
HHHHHHHCCCCCEEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]