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The map label for this gene is tig [H]

Identifier: 57237198

GI number: 57237198

Start: 186147

End: 187481

Strand: Reverse

Name: tig [H]

Synonym: CJE0186

Alternate gene names: 57237198

Gene position: 187481-186147 (Counterclockwise)

Preceding gene: 57237201

Following gene: 57237197

Centisome position: 10.55

GC content: 30.11

Gene sequence:

>1335_bases
ATGGAAGTAAAGGCAAAGCAACTAGATTCTGTTAATGCGACTGCTAGTGTAAAAATTCCTTCAGGAATGATCAAAAGTGA
AGTAGAAAATTTAGCAAAAAAAGCTTCTAAAAGTGTAAAAATGGATGGGTTTAGACCAGGAAAAGTTCCTGTAAGTGCCG
TACTTAAAAGATATGAAAGAGAGCTCACTCAAGATGCAGAACAAAATCTTTTTAAATCCGCTGTAAATAGCGCTTTACAA
GAACTTAAAAAAGAAAATAAAGAGCTTGTAGGTGAGCCATATTTTGAAAAATTTGATCGTAAAGATGGAGAAATTATTGC
TGAATTAATACTTTCTTTTAAACCTGAAATCAAACTTGAGGGTTATGAAAAACTCATCCCTGAATATCAAACTCCAAAAG
TAAGTAAAAAAGAAATTGATGAGAAAAAAGATGAACTTTTAAAGCGTTTTGCTACTCCTGAAGCTATAAAAACAAAAAGA
GCTTTAAAAGAAGGTGATTTTGCAAAATTTGATTTTGAAGGTTTTGTAGATGATAAAGCTTTTGAAGGTGGTAAAGCTGA
AAATTATGTTTTAGAAATTGGTTCTAAGCAATTTATTCCAGGTTTTGAAGATGGTATGGTGGGAATGAAAATAGGTGAAG
AAAAAGATATCAAAGTAACTTTCCCTAAAGAATACGGCGCAGCGCATTTAGCGGGTAAAGATGCAGTATTTAAAGTAAAA
CTACATGAAATTCAAGAGCTTAAAATTCCAGAACTTGATGATGAAATGCTTAAAAAACTTTTACCAGGAGAAGAAAAAGC
TAGTGTTGAAGTTTTAGATGAAAAATTAAAAGAGCAAATCAAAAATGAAAAACTTTTCAAACTTGTAAATGATGAATTAA
AAGGCAAATTTGCAGATGCTTTGATTGAAAAATATAATTTTGATTTACCTAAAGGCATAGTAGAACAAGAAACTGATATG
CAGATGCGTGCGGCTTTTAATACTTTTAGCGAAAAAGAAATTGAAGAGCTTAAAGCAAGTAAAGAAAAATATCAAGAAAA
ACGTGATTCTTTTAAAGAAGAAGCACAAAAAAGTGTAAAACTTACTTTTATCATTGATGAACTTGCAAAATTGCGTAAGA
TTGAAGTAAATGATCAAGAATTGATTCAAGCGATTTATTTTGAAGCATATCGCTATGGTATGAATCCAAAAGAGCATTTA
GAAAACTATAAAAAACAAGGAGCTTTGCCTGCTGTGAAAATGGCTTTAATTGAAGAAAAACTTTTTAATGATATTTTTAT
ACCAAAAACAGAAAAATCGGAAAAAGTGAGCAAAAAAGAGAAAGAAGATAAATAA

Upstream 100 bases:

>100_bases
TATTGCAATTTTATCATAAATTACTTTAAAATTTTATGCAAGTAATAAGGAAAAATTTGCTATCATTGAAGGCAAATTTT
ATTTTATAAGGAAAAAAACT

Downstream 100 bases:

>100_bases
TGTTTATTCCTTATGTTATTGAAAAATCTAGTCGTGGTGAAAGAAGTTATGACATTTATTCACGCCTTTTAAAAGATAGA
ATTATTATGCTAAGTGGTGA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MEVKAKQLDSVNATASVKIPSGMIKSEVENLAKKASKSVKMDGFRPGKVPVSAVLKRYERELTQDAEQNLFKSAVNSALQ
ELKKENKELVGEPYFEKFDRKDGEIIAELILSFKPEIKLEGYEKLIPEYQTPKVSKKEIDEKKDELLKRFATPEAIKTKR
ALKEGDFAKFDFEGFVDDKAFEGGKAENYVLEIGSKQFIPGFEDGMVGMKIGEEKDIKVTFPKEYGAAHLAGKDAVFKVK
LHEIQELKIPELDDEMLKKLLPGEEKASVEVLDEKLKEQIKNEKLFKLVNDELKGKFADALIEKYNFDLPKGIVEQETDM
QMRAAFNTFSEKEIEELKASKEKYQEKRDSFKEEAQKSVKLTFIIDELAKLRKIEVNDQELIQAIYFEAYRYGMNPKEHL
ENYKKQGALPAVKMALIEEKLFNDIFIPKTEKSEKVSKKEKEDK

Sequences:

>Translated_444_residues
MEVKAKQLDSVNATASVKIPSGMIKSEVENLAKKASKSVKMDGFRPGKVPVSAVLKRYERELTQDAEQNLFKSAVNSALQ
ELKKENKELVGEPYFEKFDRKDGEIIAELILSFKPEIKLEGYEKLIPEYQTPKVSKKEIDEKKDELLKRFATPEAIKTKR
ALKEGDFAKFDFEGFVDDKAFEGGKAENYVLEIGSKQFIPGFEDGMVGMKIGEEKDIKVTFPKEYGAAHLAGKDAVFKVK
LHEIQELKIPELDDEMLKKLLPGEEKASVEVLDEKLKEQIKNEKLFKLVNDELKGKFADALIEKYNFDLPKGIVEQETDM
QMRAAFNTFSEKEIEELKASKEKYQEKRDSFKEEAQKSVKLTFIIDELAKLRKIEVNDQELIQAIYFEAYRYGMNPKEHL
ENYKKQGALPAVKMALIEEKLFNDIFIPKTEKSEKVSKKEKEDK
>Mature_444_residues
MEVKAKQLDSVNATASVKIPSGMIKSEVENLAKKASKSVKMDGFRPGKVPVSAVLKRYERELTQDAEQNLFKSAVNSALQ
ELKKENKELVGEPYFEKFDRKDGEIIAELILSFKPEIKLEGYEKLIPEYQTPKVSKKEIDEKKDELLKRFATPEAIKTKR
ALKEGDFAKFDFEGFVDDKAFEGGKAENYVLEIGSKQFIPGFEDGMVGMKIGEEKDIKVTFPKEYGAAHLAGKDAVFKVK
LHEIQELKIPELDDEMLKKLLPGEEKASVEVLDEKLKEQIKNEKLFKLVNDELKGKFADALIEKYNFDLPKGIVEQETDM
QMRAAFNTFSEKEIEELKASKEKYQEKRDSFKEEAQKSVKLTFIIDELAKLRKIEVNDQELIQAIYFEAYRYGMNPKEHL
ENYKKQGALPAVKMALIEEKLFNDIFIPKTEKSEKVSKKEKEDK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=407, Percent_Identity=30.2211302211302, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 50968; Mature: 50968

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKAKQLDSVNATASVKIPSGMIKSEVENLAKKASKSVKMDGFRPGKVPVSAVLKRYER
CCCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
ELTQDAEQNLFKSAVNSALQELKKENKELVGEPYFEKFDRKDGEIIAELILSFKPEIKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCEEC
GYEKLIPEYQTPKVSKKEIDEKKDELLKRFATPEAIKTKRALKEGDFAKFDFEGFVDDKA
CHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEECCCCCCCCHH
FEGGKAENYVLEIGSKQFIPGFEDGMVGMKIGEEKDIKVTFPKEYGAAHLAGKDAVFKVK
CCCCCCCCEEEEECCCCCCCCCCCCCEECEECCCCCEEEECCHHHCCHHCCCCCCEEEEE
LHEIQELKIPELDDEMLKKLLPGEEKASVEVLDEKLKEQIKNEKLFKLVNDELKGKFADA
HHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIEKYNFDLPKGIVEQETDMQMRAAFNTFSEKEIEELKASKEKYQEKRDSFKEEAQKSVK
HHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTFIIDELAKLRKIEVNDQELIQAIYFEAYRYGMNPKEHLENYKKQGALPAVKMALIEEK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LFNDIFIPKTEKSEKVSKKEKEDK
HHHCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MEVKAKQLDSVNATASVKIPSGMIKSEVENLAKKASKSVKMDGFRPGKVPVSAVLKRYER
CCCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
ELTQDAEQNLFKSAVNSALQELKKENKELVGEPYFEKFDRKDGEIIAELILSFKPEIKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCEEC
GYEKLIPEYQTPKVSKKEIDEKKDELLKRFATPEAIKTKRALKEGDFAKFDFEGFVDDKA
CHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEECCCCCCCCHH
FEGGKAENYVLEIGSKQFIPGFEDGMVGMKIGEEKDIKVTFPKEYGAAHLAGKDAVFKVK
CCCCCCCCEEEEECCCCCCCCCCCCCEECEECCCCCEEEECCHHHCCHHCCCCCCEEEEE
LHEIQELKIPELDDEMLKKLLPGEEKASVEVLDEKLKEQIKNEKLFKLVNDELKGKFADA
HHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIEKYNFDLPKGIVEQETDMQMRAAFNTFSEKEIEELKASKEKYQEKRDSFKEEAQKSVK
HHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTFIIDELAKLRKIEVNDQELIQAIYFEAYRYGMNPKEHLENYKKQGALPAVKMALIEEK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LFNDIFIPKTEKSEKVSKKEKEDK
HHHCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA