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The map label for this gene is moaA [H]

Identifier: 57237169

GI number: 57237169

Start: 159994

End: 160956

Strand: Reverse

Name: moaA [H]

Synonym: CJE0157

Alternate gene names: 57237169

Gene position: 160956-159994 (Counterclockwise)

Preceding gene: 57237170

Following gene: 57237168

Centisome position: 9.05

GC content: 29.39

Gene sequence:

>963_bases
ATGCTTATAGATCAATTCGGTAGAAAGATAAATTATCTTAGAATTTCAGTTACACAAAGATGTAACTTTCGCTGCCTTTA
TTGTATGCCAAAAATTCCTTTCGATTATCAGCCAAAAGAAAATTTATTAAGCTTCGAAGAACTTTTTTTATTTGTTAAAG
CAGCTATGGACGAAGGAATTGAAAAAATCCGTATAACAGGTGGAGAACCTTTATTAAGAAAAGATTTAAGTATTTTTATA
AAAATGATTAGCGATTATAAAAGCGATATTGACCTTGCTATAACTACAAATGGATTTTTACTAAAAGACTTTGCTAAAGA
CCTAAAAAATGCTGGACTTAAACGACTTAATATTTCACTTGATACCCTAGATCACAAAAAAGCAAAAACTCTTGCGCAAA
AAGATGTATTAGATAGTGTTTTATCTGGAATTGATGAAGCTTTAAATCTAGATTTGAAAGTAAAACTTAATACTGTAGCT
CTTAAAAATTTAAACGATGATGAACTGATATCTCTTTTGGAATTTGCAAAATCAAAAAAAGCTCAAATTCGCTTTATAGA
ATTTATGGAAAATACTCATGCTTATGGTAAATTACAAGGATTAAAAAGAGATGAAATTATACAAATTTTGAGTCAAAAAT
ATCAAATTCAACTTATAAAAAAAGATGAAAAAGCACCTGTGAGTATTTACAAGGCAGATGATTATGAATTTGGAATTATA
GATCCTCATAGCCATGAATTTTGCGATTCTTGCAATCGCATACGCTTAAGTGCTGAAGGCTTGTTAATACCTTGCCTTTA
TTTTGATGAAGCATTAAGCATAAAAGAAGCGGTTCGAAAAGGTGATATTAAAGCCGCTGTAGAAATATTACAAGAAGTAT
TAAGAAATAAACCAGAAAAAAATAAATGGAGTGTTGTTGATAATGAAACCTCATCTCGTGCTTTTTATCAAACTGGAGGT
TAA

Upstream 100 bases:

>100_bases
AAAAAGCATAGAACAAATCGCTAAAGATTTACGCATTGGCACTGGAAATGTAGAACTTGTCTTAAAATTTAAGAAATTAA
TAAAATAGAATGGAAAATAA

Downstream 100 bases:

>100_bases
TTTTGCAACTTGTTGAAAGTTTCTTAAGCATTCAAGGAGAAGGCAAATATAATGGAAAATTAGCTATTTTTATGCGTTTT
GCTGGTTGCAATTTCAACTG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MLIDQFGRKINYLRISVTQRCNFRCLYCMPKIPFDYQPKENLLSFEELFLFVKAAMDEGIEKIRITGGEPLLRKDLSIFI
KMISDYKSDIDLAITTNGFLLKDFAKDLKNAGLKRLNISLDTLDHKKAKTLAQKDVLDSVLSGIDEALNLDLKVKLNTVA
LKNLNDDELISLLEFAKSKKAQIRFIEFMENTHAYGKLQGLKRDEIIQILSQKYQIQLIKKDEKAPVSIYKADDYEFGII
DPHSHEFCDSCNRIRLSAEGLLIPCLYFDEALSIKEAVRKGDIKAAVEILQEVLRNKPEKNKWSVVDNETSSRAFYQTGG

Sequences:

>Translated_320_residues
MLIDQFGRKINYLRISVTQRCNFRCLYCMPKIPFDYQPKENLLSFEELFLFVKAAMDEGIEKIRITGGEPLLRKDLSIFI
KMISDYKSDIDLAITTNGFLLKDFAKDLKNAGLKRLNISLDTLDHKKAKTLAQKDVLDSVLSGIDEALNLDLKVKLNTVA
LKNLNDDELISLLEFAKSKKAQIRFIEFMENTHAYGKLQGLKRDEIIQILSQKYQIQLIKKDEKAPVSIYKADDYEFGII
DPHSHEFCDSCNRIRLSAEGLLIPCLYFDEALSIKEAVRKGDIKAAVEILQEVLRNKPEKNKWSVVDNETSSRAFYQTGG
>Mature_320_residues
MLIDQFGRKINYLRISVTQRCNFRCLYCMPKIPFDYQPKENLLSFEELFLFVKAAMDEGIEKIRITGGEPLLRKDLSIFI
KMISDYKSDIDLAITTNGFLLKDFAKDLKNAGLKRLNISLDTLDHKKAKTLAQKDVLDSVLSGIDEALNLDLKVKLNTVA
LKNLNDDELISLLEFAKSKKAQIRFIEFMENTHAYGKLQGLKRDEIIQILSQKYQIQLIKKDEKAPVSIYKADDYEFGII
DPHSHEFCDSCNRIRLSAEGLLIPCLYFDEALSIKEAVRKGDIKAAVEILQEVLRNKPEKNKWSVVDNETSSRAFYQTGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=301, Percent_Identity=34.5514950166113, Blast_Score=184, Evalue=7e-47,
Organism=Homo sapiens, GI22027536, Length=301, Percent_Identity=34.5514950166113, Blast_Score=184, Evalue=8e-47,
Organism=Escherichia coli, GI1786999, Length=317, Percent_Identity=31.8611987381703, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17561210, Length=325, Percent_Identity=35.3846153846154, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI32566331, Length=306, Percent_Identity=35.6209150326797, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI32566329, Length=325, Percent_Identity=34.4615384615385, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI28574490, Length=282, Percent_Identity=33.6879432624113, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI28574488, Length=282, Percent_Identity=33.6879432624113, Blast_Score=185, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 36656; Mature: 36656

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDQFGRKINYLRISVTQRCNFRCLYCMPKIPFDYQPKENLLSFEELFLFVKAAMDEGI
CCCHHHCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
EKIRITGGEPLLRKDLSIFIKMISDYKSDIDLAITTNGFLLKDFAKDLKNAGLKRLNISL
CEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEHHHHHHHHHHCCCEEEEEEE
DTLDHKKAKTLAQKDVLDSVLSGIDEALNLDLKVKLNTVALKNLNDDELISLLEFAKSKK
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCHHHHHHHHHHHHCCC
AQIRFIEFMENTHAYGKLQGLKRDEIIQILSQKYQIQLIKKDEKAPVSIYKADDYEFGII
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCEEEECC
DPHSHEFCDSCNRIRLSAEGLLIPCLYFDEALSIKEAVRKGDIKAAVEILQEVLRNKPEK
CCCCCHHHCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
NKWSVVDNETSSRAFYQTGG
CCCEEECCCCCCCEEEECCC
>Mature Secondary Structure
MLIDQFGRKINYLRISVTQRCNFRCLYCMPKIPFDYQPKENLLSFEELFLFVKAAMDEGI
CCCHHHCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
EKIRITGGEPLLRKDLSIFIKMISDYKSDIDLAITTNGFLLKDFAKDLKNAGLKRLNISL
CEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEHHHHHHHHHHCCCEEEEEEE
DTLDHKKAKTLAQKDVLDSVLSGIDEALNLDLKVKLNTVALKNLNDDELISLLEFAKSKK
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCHHHHHHHHHHHHCCC
AQIRFIEFMENTHAYGKLQGLKRDEIIQILSQKYQIQLIKKDEKAPVSIYKADDYEFGII
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCCEEEEECCCEEEECC
DPHSHEFCDSCNRIRLSAEGLLIPCLYFDEALSIKEAVRKGDIKAAVEILQEVLRNKPEK
CCCCCHHHCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
NKWSVVDNETSSRAFYQTGG
CCCEEECCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA