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The map label for this gene is cdtA [H]

Identifier: 57237087

GI number: 57237087

Start: 89268

End: 90074

Strand: Reverse

Name: cdtA [H]

Synonym: CJE0075

Alternate gene names: 57237087

Gene position: 90074-89268 (Counterclockwise)

Preceding gene: 57237092

Following gene: 57237086

Centisome position: 5.07

GC content: 34.2

Gene sequence:

>807_bases
ATGCAAAAAATTATAGTTTTTATTTTATGTTGTTTTATGACTTTTTTTCTTTATGCATGTTCTTCTAAATTTGAAAATGT
AAATCCTTTGGGGCGTTCATTTGGCGAATTTGAAGATACTGATCCTTTAAAACTAGGACTTGAACCTACTTTTCCTACCA
ATCAAGAAATTCCAAGTTTAATTAGCGGTGCTGATTTAGTACCTATTACTCCTATTACCCCACCTTTAACTAGAACAAGC
AATAGTGCCAACAATAATGCAGTAAATGGGATCAATCCTCGCTTTAAAGACGAAGCTTTTAATGATGTTTTAATTTTTGA
AAATCGCCCTGCGGTTTCTGATTTTTTAACCATTTTAGGCCCTAGCGGAGCAGCTTTAACGGTTTGGGCTTTAGCACAAG
GAAATTGGATTTGGGGCTATACTTTAATCGATAGTAAAGGATTTGGCGATGCTAGAGTTTGGCAACTTTTGCTTTATCCT
AATGATTTTGCAATGATTAAAAATGCCAAAACCAATACTTGTCTTAATGCTTATGGTAATGGAATTGTCCATTATCCTTG
CGATGCAAGCAATCACGCACAAATGTGGAAACTTATCCCTATGAGCAATACAGCGGTTCAAATTAAAAATTTAGGAAATG
GAAAATGCATACAAGCACCTATTACAAATCTTTATGGTGATTTTCACAAGGTTTTTAAAATTTTTACCGTAGAGTGTGCA
AAAAAAGATAATTTTGATCAACAATGGTTTTTAACTACTCCGCCTTTTACTGCAAAACCTTTATATCGCCAAGGAGAGGT
ACGATGA

Upstream 100 bases:

>100_bases
TTTAAAAACAACAAAATAAAACATTTTTAAAAAGCGGAAAATTATAATGAAATTTATGTTATTATTTTCTTAAAAATTTA
AATACATATCAAGGTTTTTA

Downstream 100 bases:

>100_bases
AAAAAATTATATGTTTATTTTTATCTTTTAACCTTGCTTTTGCAAATTTAGAAAATTTTAATGTTGGCACTTGGAATTTG
CAAGGCTCATCCGCAGCCAC

Product: cytolethal distending toxin, subunit A

Products: NA

Alternate protein names: CDT A [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MQKIIVFILCCFMTFFLYACSSKFENVNPLGRSFGEFEDTDPLKLGLEPTFPTNQEIPSLISGADLVPITPITPPLTRTS
NSANNNAVNGINPRFKDEAFNDVLIFENRPAVSDFLTILGPSGAALTVWALAQGNWIWGYTLIDSKGFGDARVWQLLLYP
NDFAMIKNAKTNTCLNAYGNGIVHYPCDASNHAQMWKLIPMSNTAVQIKNLGNGKCIQAPITNLYGDFHKVFKIFTVECA
KKDNFDQQWFLTTPPFTAKPLYRQGEVR

Sequences:

>Translated_268_residues
MQKIIVFILCCFMTFFLYACSSKFENVNPLGRSFGEFEDTDPLKLGLEPTFPTNQEIPSLISGADLVPITPITPPLTRTS
NSANNNAVNGINPRFKDEAFNDVLIFENRPAVSDFLTILGPSGAALTVWALAQGNWIWGYTLIDSKGFGDARVWQLLLYP
NDFAMIKNAKTNTCLNAYGNGIVHYPCDASNHAQMWKLIPMSNTAVQIKNLGNGKCIQAPITNLYGDFHKVFKIFTVECA
KKDNFDQQWFLTTPPFTAKPLYRQGEVR
>Mature_268_residues
MQKIIVFILCCFMTFFLYACSSKFENVNPLGRSFGEFEDTDPLKLGLEPTFPTNQEIPSLISGADLVPITPITPPLTRTS
NSANNNAVNGINPRFKDEAFNDVLIFENRPAVSDFLTILGPSGAALTVWALAQGNWIWGYTLIDSKGFGDARVWQLLLYP
NDFAMIKNAKTNTCLNAYGNGIVHYPCDASNHAQMWKLIPMSNTAVQIKNLGNGKCIQAPITNLYGDFHKVFKIFTVECA
KKDNFDQQWFLTTPPFTAKPLYRQGEVR

Specific function: CDTs are cytotoxins which induce cell distension, growth arrest in G2/M phase, nucleus swelling, and chromatin fragmentation in HeLa cells [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ricin B-type lectin domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015957
- InterPro:   IPR003558
- InterPro:   IPR008997
- InterPro:   IPR000772 [H]

Pfam domain/function: PF03498 CDtoxinA [H]

EC number: NA

Molecular weight: Translated: 29948; Mature: 29948

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50231 RICIN_B_LECTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKIIVFILCCFMTFFLYACSSKFENVNPLGRSFGEFEDTDPLKLGLEPTFPTNQEIPSL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHH
ISGADLVPITPITPPLTRTSNSANNNAVNGINPRFKDEAFNDVLIFENRPAVSDFLTILG
HCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHC
PSGAALTVWALAQGNWIWGYTLIDSKGFGDARVWQLLLYPNDFAMIKNAKTNTCLNAYGN
CCCCEEEEEEEECCCEEEEEEEEECCCCCCHHEEEEEECCCCEEEEECCCCCHHHHHCCC
GIVHYPCDASNHAQMWKLIPMSNTAVQIKNLGNGKCIQAPITNLYGDFHKVFKIFTVECA
CEEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHEEC
KKDNFDQQWFLTTPPFTAKPLYRQGEVR
CCCCCCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQKIIVFILCCFMTFFLYACSSKFENVNPLGRSFGEFEDTDPLKLGLEPTFPTNQEIPSL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHH
ISGADLVPITPITPPLTRTSNSANNNAVNGINPRFKDEAFNDVLIFENRPAVSDFLTILG
HCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHC
PSGAALTVWALAQGNWIWGYTLIDSKGFGDARVWQLLLYPNDFAMIKNAKTNTCLNAYGN
CCCCEEEEEEEECCCEEEEEEEEECCCCCCHHEEEEEECCCCEEEEECCCCCHHHHHCCC
GIVHYPCDASNHAQMWKLIPMSNTAVQIKNLGNGKCIQAPITNLYGDFHKVFKIFTVECA
CEEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHEEC
KKDNFDQQWFLTTPPFTAKPLYRQGEVR
CCCCCCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]