Click here to switch to the map view.

The map label for this gene is sppA [C]

Identifier: 57237078

GI number: 57237078

Start: 80049

End: 80945

Strand: Direct

Name: sppA [C]

Synonym: CJE0065

Alternate gene names: 57237078

Gene position: 80049-80945 (Clockwise)

Preceding gene: 57237077

Following gene: 57237079

Centisome position: 4.5

GC content: 32.78

Gene sequence:

>897_bases
ATGCAAATTTTAAAATCATTTTTTAAGGCTTTAGGATGCGGTATAAAATTTATCAATACTTATTTTAAAACCTTTGTTTT
ATTGTTGATTGTTATATGGATTTTAATGCCTAGTGCAAATTCAAGCTCCAATCTTGCAAATTTAGAACGCATTGATTTAA
AGGGTGAAATTTTTGACAGCTCTGCGGTTTTGGAAAAAATTATCAATGCTAAAAATGATAGCAATATTAAAGGAGTGCTT
TTTGTTATAGACTCTCCTGGTGGAGCTTTTGCTCCTAGTATGGAACTAGCTCTAGCTATAAAGGATTTAAAAATTAAAAA
GCCTGTGCTTGTTTATGCAAGCGGAACTATGGCAAGCGGAAGTTATTTAGCAGGAGTTGGAGCTAATAAAATTTTAGCCA
ATCCAGCAAGTTTTATAGGCTCTATCGGTGTGATTATGCAAGGAGCTGATTTAAGCGGATTGGCTAATAAACTCGGTATT
AAAGAACAAACTATTCAAGCGGGTGAATTTAAAAGTGCTGGAACTTTTGCAAGAGCTTGGAATGAGAATGAAAGAAATTT
TTTACAGGGTTTAATTGATCAAAGTTATGATCTTTTTACAGGTTTTGTTGTCAAGGAAAGAGCATTAGATCTTAATAAAA
AAGATCAATGGGCAAATGCAAGAGTGTTTTTAGCTGCCAAGGCTAAGGAGCTTGGTCTTATAGATGAGCTTAGTAATTAT
GAAAATGCTAAAAAGGAGCTTGAAAAACTTGCTAATGTTTCAAATCCTGTTTGGAAAGAAGAAGATAAGATTGATAAATT
TCTTAACCGCCTAGAAGGACAAACATCAAGCTTGATAAGTAAAAGCTTAATCGAAATCGCTTATAAAACTAATAGCAGTT
TTATAAACGCTAGATAA

Upstream 100 bases:

>100_bases
TTAACGTATTTGAGCTAGGAGAATGTGATAAAAAGCAAGCTCCTTTGCAGTTTATTTTAAATGCTAAGGAAGTAGATAAA
TTATTTATAAAAGGAAAAGA

Downstream 100 bases:

>100_bases
AATATATAAATATTTTTATTTAATTTTTGTAATATTTTTATGGAATAATTATTTATATCTTAGATAAGGAGTAAAATAAT
GAAAAAATATTGTTTTTTTC

Product: signal peptide peptidase SppA, 36K type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MQILKSFFKALGCGIKFINTYFKTFVLLLIVIWILMPSANSSSNLANLERIDLKGEIFDSSAVLEKIINAKNDSNIKGVL
FVIDSPGGAFAPSMELALAIKDLKIKKPVLVYASGTMASGSYLAGVGANKILANPASFIGSIGVIMQGADLSGLANKLGI
KEQTIQAGEFKSAGTFARAWNENERNFLQGLIDQSYDLFTGFVVKERALDLNKKDQWANARVFLAAKAKELGLIDELSNY
ENAKKELEKLANVSNPVWKEEDKIDKFLNRLEGQTSSLISKSLIEIAYKTNSSFINAR

Sequences:

>Translated_298_residues
MQILKSFFKALGCGIKFINTYFKTFVLLLIVIWILMPSANSSSNLANLERIDLKGEIFDSSAVLEKIINAKNDSNIKGVL
FVIDSPGGAFAPSMELALAIKDLKIKKPVLVYASGTMASGSYLAGVGANKILANPASFIGSIGVIMQGADLSGLANKLGI
KEQTIQAGEFKSAGTFARAWNENERNFLQGLIDQSYDLFTGFVVKERALDLNKKDQWANARVFLAAKAKELGLIDELSNY
ENAKKELEKLANVSNPVWKEEDKIDKFLNRLEGQTSSLISKSLIEIAYKTNSSFINAR
>Mature_298_residues
MQILKSFFKALGCGIKFINTYFKTFVLLLIVIWILMPSANSSSNLANLERIDLKGEIFDSSAVLEKIINAKNDSNIKGVL
FVIDSPGGAFAPSMELALAIKDLKIKKPVLVYASGTMASGSYLAGVGANKILANPASFIGSIGVIMQGADLSGLANKLGI
KEQTIQAGEFKSAGTFARAWNENERNFLQGLIDQSYDLFTGFVVKERALDLNKKDQWANARVFLAAKAKELGLIDELSNY
ENAKKELEKLANVSNPVWKEEDKIDKFLNRLEGQTSSLISKSLIEIAYKTNSSFINAR

Specific function: Digestion Of The Cleaved Signal Peptides. This Activity Is Necessary To Maintain Proper Secretion Of Mature Proteins Across The Membrane. [C]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=225, Percent_Identity=24.4444444444444, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787527, Length=192, Percent_Identity=27.0833333333333, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.- [C]

Molecular weight: Translated: 32630; Mature: 32630

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQILKSFFKALGCGIKFINTYFKTFVLLLIVIWILMPSANSSSNLANLERIDLKGEIFDS
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEECCCCCCCCH
SAVLEKIINAKNDSNIKGVLFVIDSPGGAFAPSMELALAIKDLKIKKPVLVYASGTMASG
HHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCC
SYLAGVGANKILANPASFIGSIGVIMQGADLSGLANKLGIKEQTIQAGEFKSAGTFARAW
CEEECCCCCHHHCCHHHHHHHHHHHEECCCHHHHHHHHCCCHHHHCCCCCCCCCHHHHHC
NENERNFLQGLIDQSYDLFTGFVVKERALDLNKKDQWANARVFLAAKAKELGLIDELSNY
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHCHHHHHHHH
ENAKKELEKLANVSNPVWKEEDKIDKFLNRLEGQTSSLISKSLIEIAYKTNSSFINAR
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEECCC
>Mature Secondary Structure
MQILKSFFKALGCGIKFINTYFKTFVLLLIVIWILMPSANSSSNLANLERIDLKGEIFDS
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEECCCCCCCCH
SAVLEKIINAKNDSNIKGVLFVIDSPGGAFAPSMELALAIKDLKIKKPVLVYASGTMASG
HHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCC
SYLAGVGANKILANPASFIGSIGVIMQGADLSGLANKLGIKEQTIQAGEFKSAGTFARAW
CEEECCCCCHHHCCHHHHHHHHHHHEECCCHHHHHHHHCCCHHHHCCCCCCCCCHHHHHC
NENERNFLQGLIDQSYDLFTGFVVKERALDLNKKDQWANARVFLAAKAKELGLIDELSNY
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHCHHHHHHHH
ENAKKELEKLANVSNPVWKEEDKIDKFLNRLEGQTSSLISKSLIEIAYKTNSSFINAR
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]