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The map label for this gene is fliA [H]

Identifier: 57237071

GI number: 57237071

Start: 74415

End: 75107

Strand: Reverse

Name: fliA [H]

Synonym: CJE0058

Alternate gene names: 57237071

Gene position: 75107-74415 (Counterclockwise)

Preceding gene: 57237072

Following gene: 57237070

Centisome position: 4.22

GC content: 32.61

Gene sequence:

>693_bases
ATGCTAAAAGAGCCGCCTAAAGCTTATGCACAAATGCTAAAGAAAGAGCAAGATGAGCTTGTGCTTTCTTACATGCCAGC
TCTGCGTGCTATGGCTTTTAGACTAAAAGAACGCTTGCCTTCAAGTATAGATGTTAATGATCTTATTAGCATAGGCGTTG
AAGAAATGATAAAGCTTTCACGCCGTTACGATAAAGAACAAAATGATAACTTCTGGGGTTTTGCAAGAAAAAGAGTTAAC
GGCTCTATGCTTGATTATCTTAGAAGTCTTGATGTAATGAGTCGAAATAATCGTAAAATTATTAAAGATATTGATGCAAT
CATGGATGAATATTTTTTAGAACATGAATGCGAACCTGATGATGAGTATCTAGCCAAAAAACTTGATTTAGATGTAGAAA
AAATCAAAGAAGTAAGAACAGCTCATGCTATTAGCTATACCCTGCCCATTGATGAGCAAATCGAACTTTATAATGAAGAC
AATACTTTAGAAAAAATTGAAAAAGAAGAACTTTTAGAAAAAATTCACGAAGTGCTAGATGATCTTAAAGAGCGCGATCA
GTTAATTATTCAGCTTTATTATTATGAAGAATTAAGCCTAAAAGAAATCAGTGAAATTTTACAAATCAGTGAAAGCAGAA
TTTCACAAATTCACAAAAAACTTCTTAAAAAACTTAGAGAAAGGCTAGTGTAA

Upstream 100 bases:

>100_bases
GATTTAGTTATTCGTGAAAAGAAAATGGATATAATTTTAGAAAAGCTCAATGAAGAAAGAGAAGAGCTAAAGAAAAATGA
ATTCAAAGAAAGACGAAGAA

Downstream 100 bases:

>100_bases
TGGCTGAGATACTCTCCCAAGAAGAAATTGATGCTCTACTTGAAGTTGTCGATGATAACACCGACACACCCATAGCCTCA
AATTCAAAAGATGAAAAAGA

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MLKEPPKAYAQMLKKEQDELVLSYMPALRAMAFRLKERLPSSIDVNDLISIGVEEMIKLSRRYDKEQNDNFWGFARKRVN
GSMLDYLRSLDVMSRNNRKIIKDIDAIMDEYFLEHECEPDDEYLAKKLDLDVEKIKEVRTAHAISYTLPIDEQIELYNED
NTLEKIEKEELLEKIHEVLDDLKERDQLIIQLYYYEELSLKEISEILQISESRISQIHKKLLKKLRERLV

Sequences:

>Translated_230_residues
MLKEPPKAYAQMLKKEQDELVLSYMPALRAMAFRLKERLPSSIDVNDLISIGVEEMIKLSRRYDKEQNDNFWGFARKRVN
GSMLDYLRSLDVMSRNNRKIIKDIDAIMDEYFLEHECEPDDEYLAKKLDLDVEKIKEVRTAHAISYTLPIDEQIELYNED
NTLEKIEKEELLEKIHEVLDDLKERDQLIIQLYYYEELSLKEISEILQISESRISQIHKKLLKKLRERLV
>Mature_230_residues
MLKEPPKAYAQMLKKEQDELVLSYMPALRAMAFRLKERLPSSIDVNDLISIGVEEMIKLSRRYDKEQNDNFWGFARKRVN
GSMLDYLRSLDVMSRNNRKIIKDIDAIMDEYFLEHECEPDDEYLAKKLDLDVEKIKEVRTAHAISYTLPIDEQIELYNED
NTLEKIEKEELLEKIHEVLDDLKERDQLIIQLYYYEELSLKEISEILQISESRISQIHKKLLKKLRERLV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellar operon [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=217, Percent_Identity=32.258064516129, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27455; Mature: 27455

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEPPKAYAQMLKKEQDELVLSYMPALRAMAFRLKERLPSSIDVNDLISIGVEEMIKLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RRYDKEQNDNFWGFARKRVNGSMLDYLRSLDVMSRNNRKIIKDIDAIMDEYFLEHECEPD
HHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
DEYLAKKLDLDVEKIKEVRTAHAISYTLPIDEQIELYNEDNTLEKIEKEELLEKIHEVLD
HHHHHHHHCCCHHHHHHHHHHHHHHEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DLKERDQLIIQLYYYEELSLKEISEILQISESRISQIHKKLLKKLRERLV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKEPPKAYAQMLKKEQDELVLSYMPALRAMAFRLKERLPSSIDVNDLISIGVEEMIKLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RRYDKEQNDNFWGFARKRVNGSMLDYLRSLDVMSRNNRKIIKDIDAIMDEYFLEHECEPD
HHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
DEYLAKKLDLDVEKIKEVRTAHAISYTLPIDEQIELYNEDNTLEKIEKEELLEKIHEVLD
HHHHHHHHCCCHHHHHHHHHHHHHHEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DLKERDQLIIQLYYYEELSLKEISEILQISESRISQIHKKLLKKLRERLV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730257 [H]