Click here to switch to the map view.

The map label for this gene is fliM [H]

Identifier: 57237070

GI number: 57237070

Start: 73336

End: 74415

Strand: Reverse

Name: fliM [H]

Synonym: CJE0057

Alternate gene names: 57237070

Gene position: 74415-73336 (Counterclockwise)

Preceding gene: 57237071

Following gene: 57237069

Centisome position: 4.19

GC content: 33.98

Gene sequence:

>1080_bases
ATGGCTGAGATACTCTCCCAAGAAGAAATTGATGCTCTACTTGAAGTTGTCGATGATAACACCGACACACCCATAGCCTC
AAATTCAAAAGATGAAAAAGATGAAAGAAATATCGTTGTATATGATTTTAAGCGTCCCAACAGAGTTTCAAAAGAACAAC
TTAGGACAATCAAAGGCATACATGATAAATTAGCAAGAAATTTAGCCTCTCAAATTTCATCGATGATGAGAAGTATTGTT
GAAACTAAACTTCATTCAGTCGATCAGATGACTTATGGAGAGTTTTTAATGTCTTTGCCAAGCCCAACAAGTTTTAACGT
TTTTTCTATCAAACCTTTAGATGGAAACTGTGTTTTAGAAATCAATCCAAGCATAGCTTTTCCTATGATAGATAGACTTT
TGGGCGGACAAGGTGATAGCTATGAAGCTTCGCGTGAGCTTACGGATATTGAGCTTAATTTGCTTGATTCTATTTTGCGT
ATCATTATGCAAAGACTTAAAGAAAGCTGGGCTACCGTAACTGAAATTTATCCTAGTATAGAAGCTAAAGAATCAAGTCC
AAATGTTGTACAAATCGTATCTCAAAATGAGATTGTTATCATGGTGGTAATGGAAATCATCATAGGAAATTCAAGCGGAA
TGGTAAATATCTGCTATCCTGTAGTACATTTAGAAAGTATATTAAGCCGTTTAGCAAACCGTGATATTATGATGGGTGAA
ACCTCAGCCAAAAAATCACGCAACAAAGAACTTAAAACTTTAATCGGTCGTGCTGAAGTGGTATATGAGGCAATTTTAGG
AAAAACCTTAATTAATGTTCATGAATTTTTAGAACTTAAACAAGGCGATATCTTAAGACTTGATAGAGAAGCAGATGATA
AAGCTATAGTAAGCATCGATAAAAAAGATGTTTTCTTAGCACAAATTGGATTGCATCGTTTTAGAAAATCTATTAAAATT
TTAGAACTTATACGCACAGATAAAGATGAAATCAAAGAAATTTTGGAAAAATACGAAGAAGAGCGTAAAGCTAAAGCTAG
TGTTTATGATGAGCCTGAAGAGGAGGATGAAGAAATATGA

Upstream 100 bases:

>100_bases
TAAGCCTAAAAGAAATCAGTGAAATTTTACAAATCAGTGAAAGCAGAATTTCACAAATTCACAAAAAACTTCTTAAAAAA
CTTAGAGAAAGGCTAGTGTA

Downstream 100 bases:

>100_bases
TCAATGATTTTTTAAAAATGTTTACAAATGAATGCATCAGCACCATTGAAGGTTTAACAGGAAAAAGTGCTGAATTTAGC
GAATATAAAGAATTTGATGT

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MAEILSQEEIDALLEVVDDNTDTPIASNSKDEKDERNIVVYDFKRPNRVSKEQLRTIKGIHDKLARNLASQISSMMRSIV
ETKLHSVDQMTYGEFLMSLPSPTSFNVFSIKPLDGNCVLEINPSIAFPMIDRLLGGQGDSYEASRELTDIELNLLDSILR
IIMQRLKESWATVTEIYPSIEAKESSPNVVQIVSQNEIVIMVVMEIIIGNSSGMVNICYPVVHLESILSRLANRDIMMGE
TSAKKSRNKELKTLIGRAEVVYEAILGKTLINVHEFLELKQGDILRLDREADDKAIVSIDKKDVFLAQIGLHRFRKSIKI
LELIRTDKDEIKEILEKYEEERKAKASVYDEPEEEDEEI

Sequences:

>Translated_359_residues
MAEILSQEEIDALLEVVDDNTDTPIASNSKDEKDERNIVVYDFKRPNRVSKEQLRTIKGIHDKLARNLASQISSMMRSIV
ETKLHSVDQMTYGEFLMSLPSPTSFNVFSIKPLDGNCVLEINPSIAFPMIDRLLGGQGDSYEASRELTDIELNLLDSILR
IIMQRLKESWATVTEIYPSIEAKESSPNVVQIVSQNEIVIMVVMEIIIGNSSGMVNICYPVVHLESILSRLANRDIMMGE
TSAKKSRNKELKTLIGRAEVVYEAILGKTLINVHEFLELKQGDILRLDREADDKAIVSIDKKDVFLAQIGLHRFRKSIKI
LELIRTDKDEIKEILEKYEEERKAKASVYDEPEEEDEEI
>Mature_358_residues
AEILSQEEIDALLEVVDDNTDTPIASNSKDEKDERNIVVYDFKRPNRVSKEQLRTIKGIHDKLARNLASQISSMMRSIVE
TKLHSVDQMTYGEFLMSLPSPTSFNVFSIKPLDGNCVLEINPSIAFPMIDRLLGGQGDSYEASRELTDIELNLLDSILRI
IMQRLKESWATVTEIYPSIEAKESSPNVVQIVSQNEIVIMVVMEIIIGNSSGMVNICYPVVHLESILSRLANRDIMMGET
SAKKSRNKELKTLIGRAEVVYEAILGKTLINVHEFLELKQGDILRLDREADDKAIVSIDKKDVFLAQIGLHRFRKSIKIL
ELIRTDKDEIKEILEKYEEERKAKASVYDEPEEEDEEI

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=326, Percent_Identity=26.9938650306748, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 40827; Mature: 40696

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEILSQEEIDALLEVVDDNTDTPIASNSKDEKDERNIVVYDFKRPNRVSKEQLRTIKGI
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHH
HDKLARNLASQISSMMRSIVETKLHSVDQMTYGEFLMSLPSPTSFNVFSIKPLDGNCVLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEE
INPSIAFPMIDRLLGGQGDSYEASRELTDIELNLLDSILRIIMQRLKESWATVTEIYPSI
ECCCCHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EAKESSPNVVQIVSQNEIVIMVVMEIIIGNSSGMVNICYPVVHLESILSRLANRDIMMGE
CCCCCCCCEEEEECCCCEEHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCEEECC
TSAKKSRNKELKTLIGRAEVVYEAILGKTLINVHEFLELKQGDILRLDREADDKAIVSID
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEC
KKDVFLAQIGLHRFRKSIKILELIRTDKDEIKEILEKYEEERKAKASVYDEPEEEDEEI
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AEILSQEEIDALLEVVDDNTDTPIASNSKDEKDERNIVVYDFKRPNRVSKEQLRTIKGI
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHH
HDKLARNLASQISSMMRSIVETKLHSVDQMTYGEFLMSLPSPTSFNVFSIKPLDGNCVLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEE
INPSIAFPMIDRLLGGQGDSYEASRELTDIELNLLDSILRIIMQRLKESWATVTEIYPSI
ECCCCHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EAKESSPNVVQIVSQNEIVIMVVMEIIIGNSSGMVNICYPVVHLESILSRLANRDIMMGE
CCCCCCCCEEEEECCCCEEHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCEEECC
TSAKKSRNKELKTLIGRAEVVYEAILGKTLINVHEFLELKQGDILRLDREADDKAIVSID
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEC
KKDVFLAQIGLHRFRKSIKILELIRTDKDEIKEILEKYEEERKAKASVYDEPEEEDEEI
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]