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The map label for this gene is priA [H]

Identifier: 57236987

GI number: 57236987

Start: 715332

End: 717185

Strand: Direct

Name: priA [H]

Synonym: CJE0782

Alternate gene names: 57236987

Gene position: 715332-717185 (Clockwise)

Preceding gene: 57236986

Following gene: 57236989

Centisome position: 40.24

GC content: 30.1

Gene sequence:

>1854_bases
ATGCAAAGATCTGATATAAGATATTATGAACTTGCAATTTGTGGTTTGTATTTGGATAATTTAACTTTTCATAGTTTTGA
TGAAATCAAACCTTTAACACAAGTATTAGTGGATTTAAAATCTAAAAAAAATCTTAAAGCCATCGTACTTAAAGAATGTC
AAAAACCTGATTTTAAAACTATAGAAATTAAAGAAATTACAGGGTATTTTCTAACTCCTTTGCAGTTTGAATTGGCAAAT
TTTATTGTTTATTATTATGCTTCAAAACTTGGTTTTGTTTTGGGTTTTTTTGAAACTAGTCCTAAGTATGAATGTCACAA
AATGTTTTTTAAAGATACTCCTAAACTAAGCAATCAACAACAAAATGCCTTAAGTTTTCTTCAAAAAGAAAATAATTCCT
TGCTTTTTGCTGATACGGGTAGTGGCAAGACTGAAATTTATATTTCTTTGATAAAAGAGTGTTTAGAACAGGGTAAGCAA
GCTTTGCTTTTGATGCCAGAAATCGCACTTACTCCACAAATGCAAAAGCGTTTAGAAGTGTATTTTAAGGATAATTTTTT
CCTGTGGCATTCTAAAATTTCAAAGAAAAAAAAGCAAGAATATTTAGAGCGTTTTTGCAAGGGAGAAGTGCTTTTAGTAG
CGGGTGCAAGATCGGCTTTGTTTTTACCTTTTAGAAATTTAGGACTTATTGTAGTAGATGAAGAACATGATAATTCTTAT
AAGGCTTCTAACCAGCCTTTTATCAATGCAAGAGATTTAGCACTTTTTTTGGGTCAAAAAAATAATATTAAAGTGGTTTT
AGGTTCTGCAACCCCATCTTTAACGAGCTTTTATAAACAAAAAAGTTTTAGATTAAAAGGAACTTTTTTTGAGAGTAAAA
AACATTTTTTATATGATGAAAATGAGCTTGGTATAACGCCTATGCTTTTAAGTGAATTAGAAAAAAGTTTAAAACATCAA
AAGCAAGCTATAGTGTTTTTACCTACAAGGGCTAATTTTAGGCAAATCATTTGTAAGGATTGCGGAGAAACGATAAAATG
TCCTTTTTGTTCTATTGCTATGAGTATGCATAAAAAGAAAAATGTTTTAAAATGTCATTATTGTAATTATACTTCTTTAA
TAGAGCAAAATTGTCCAAGTTGCAAAGGAGAAATGTTAGAAGCTAGAAAAATGGGGACTGCTGAACTTTTAGAGCTTTTG
CAAAATGCTTTGCCTTTAGCAAAAATAGCAAAATTTGATAGCGATGAAATCACAAGTGTTAAAAAGCTTAATACTATTTT
AAAAGACTTTAATGAAAATAAGATCGATATACTTATTGGCACTTCTATGCTTGCTAAAGGACATGATTATCATAGTGTGG
ATTTGAGTGTGATTTTAGGGCTTGATGAATATTTGTTGCGTCCTAGTTTTAGAGCGAGTGAAGAAACTTTAGCTTTAGCT
ATGCAAGTTGCAGGCAGGGCAGGGCGTAAAGGCGAAGCAAGAGTACTTTTGCAAACAAAAAATAGAGCTTTTTTTGAAAG
GTATATAGAGAATTATGATGCTTTTTTAAAAGATGAGTTAGAAAATAGAAAAGATCTTTACCCCCCATTTAAACGCCTTT
TAAGAGTTCTTATAGAAGATAAAGATCAAAAAAGTGCACAAAAACTTTGTGAAAAATTTGCTTCTCAATTTAGAAATATC
AAACAGGTAGAATTGGTAGGCTATGGAATTTGTGGAGTTGAAATGCTGTATGGAAAATATCGTTTTTATTTGTTGCTTAG
AAGTGAAAATCATAAAGCTTTAGTGGCAATTGAAAATTATATTTTGCAATTTAAAAATGCAAGTGCGGATATTGATCCTA
TTGATTTTGTGTAA

Upstream 100 bases:

>100_bases
TAGGTAAAAATCACTATAGATTTGCATTAAATGCTAAAGAGATGGTTGAATTTATTTATGATTCTAAAGAAGGTTTGTTA
AAATGTATAGGAAGTTCTAG

Downstream 100 bases:

>100_bases
GTGATATGATTATTTATCTTTGTATATTTTTTCAAATTCTTTATAATATTTCCAAGAATCAATAATATGAGGATGTGTTT
GTTTTAAATGATTTAGTTCA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MQRSDIRYYELAICGLYLDNLTFHSFDEIKPLTQVLVDLKSKKNLKAIVLKECQKPDFKTIEIKEITGYFLTPLQFELAN
FIVYYYASKLGFVLGFFETSPKYECHKMFFKDTPKLSNQQQNALSFLQKENNSLLFADTGSGKTEIYISLIKECLEQGKQ
ALLLMPEIALTPQMQKRLEVYFKDNFFLWHSKISKKKKQEYLERFCKGEVLLVAGARSALFLPFRNLGLIVVDEEHDNSY
KASNQPFINARDLALFLGQKNNIKVVLGSATPSLTSFYKQKSFRLKGTFFESKKHFLYDENELGITPMLLSELEKSLKHQ
KQAIVFLPTRANFRQIICKDCGETIKCPFCSIAMSMHKKKNVLKCHYCNYTSLIEQNCPSCKGEMLEARKMGTAELLELL
QNALPLAKIAKFDSDEITSVKKLNTILKDFNENKIDILIGTSMLAKGHDYHSVDLSVILGLDEYLLRPSFRASEETLALA
MQVAGRAGRKGEARVLLQTKNRAFFERYIENYDAFLKDELENRKDLYPPFKRLLRVLIEDKDQKSAQKLCEKFASQFRNI
KQVELVGYGICGVEMLYGKYRFYLLLRSENHKALVAIENYILQFKNASADIDPIDFV

Sequences:

>Translated_617_residues
MQRSDIRYYELAICGLYLDNLTFHSFDEIKPLTQVLVDLKSKKNLKAIVLKECQKPDFKTIEIKEITGYFLTPLQFELAN
FIVYYYASKLGFVLGFFETSPKYECHKMFFKDTPKLSNQQQNALSFLQKENNSLLFADTGSGKTEIYISLIKECLEQGKQ
ALLLMPEIALTPQMQKRLEVYFKDNFFLWHSKISKKKKQEYLERFCKGEVLLVAGARSALFLPFRNLGLIVVDEEHDNSY
KASNQPFINARDLALFLGQKNNIKVVLGSATPSLTSFYKQKSFRLKGTFFESKKHFLYDENELGITPMLLSELEKSLKHQ
KQAIVFLPTRANFRQIICKDCGETIKCPFCSIAMSMHKKKNVLKCHYCNYTSLIEQNCPSCKGEMLEARKMGTAELLELL
QNALPLAKIAKFDSDEITSVKKLNTILKDFNENKIDILIGTSMLAKGHDYHSVDLSVILGLDEYLLRPSFRASEETLALA
MQVAGRAGRKGEARVLLQTKNRAFFERYIENYDAFLKDELENRKDLYPPFKRLLRVLIEDKDQKSAQKLCEKFASQFRNI
KQVELVGYGICGVEMLYGKYRFYLLLRSENHKALVAIENYILQFKNASADIDPIDFV
>Mature_617_residues
MQRSDIRYYELAICGLYLDNLTFHSFDEIKPLTQVLVDLKSKKNLKAIVLKECQKPDFKTIEIKEITGYFLTPLQFELAN
FIVYYYASKLGFVLGFFETSPKYECHKMFFKDTPKLSNQQQNALSFLQKENNSLLFADTGSGKTEIYISLIKECLEQGKQ
ALLLMPEIALTPQMQKRLEVYFKDNFFLWHSKISKKKKQEYLERFCKGEVLLVAGARSALFLPFRNLGLIVVDEEHDNSY
KASNQPFINARDLALFLGQKNNIKVVLGSATPSLTSFYKQKSFRLKGTFFESKKHFLYDENELGITPMLLSELEKSLKHQ
KQAIVFLPTRANFRQIICKDCGETIKCPFCSIAMSMHKKKNVLKCHYCNYTSLIEQNCPSCKGEMLEARKMGTAELLELL
QNALPLAKIAKFDSDEITSVKKLNTILKDFNENKIDILIGTSMLAKGHDYHSVDLSVILGLDEYLLRPSFRASEETLALA
MQVAGRAGRKGEARVLLQTKNRAFFERYIENYDAFLKDELENRKDLYPPFKRLLRVLIEDKDQKSAQKLCEKFASQFRNI
KQVELVGYGICGVEMLYGKYRFYLLLRSENHKALVAIENYILQFKNASADIDPIDFV

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=538, Percent_Identity=32.5278810408922, Blast_Score=282, Evalue=5e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 71169; Mature: 71169

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRSDIRYYELAICGLYLDNLTFHSFDEIKPLTQVLVDLKSKKNLKAIVLKECQKPDFKT
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCE
IEIKEITGYFLTPLQFELANFIVYYYASKLGFVLGFFETSPKYECHKMFFKDTPKLSNQQ
EEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHCCCCCCCCCH
QNALSFLQKENNSLLFADTGSGKTEIYISLIKECLEQGKQALLLMPEIALTPQMQKRLEV
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCHHCCHHHHHHEEE
YFKDNFFLWHSKISKKKKQEYLERFCKGEVLLVAGARSALFLPFRNLGLIVVDEEHDNSY
EECCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCEEEEECCCCCCC
KASNQPFINARDLALFLGQKNNIKVVLGSATPSLTSFYKQKSFRLKGTFFESKKHFLYDE
CCCCCCCCCHHHEEEEECCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCCEEECC
NELGITPMLLSELEKSLKHQKQAIVFLPTRANFRQIICKDCGETIKCPFCSIAMSMHKKK
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCC
NVLKCHYCNYTSLIEQNCPSCKGEMLEARKMGTAELLELLQNALPLAKIAKFDSDEITSV
CCEEEEECCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCHHHHHH
KKLNTILKDFNENKIDILIGTSMLAKGHDYHSVDLSVILGLDEYLLRPSFRASEETLALA
HHHHHHHHHCCCCEEEEEEECHHHHCCCCCCEEEEEEEECCHHHHHCCCCCCCHHHHHHH
MQVAGRAGRKGEARVLLQTKNRAFFERYIENYDAFLKDELENRKDLYPPFKRLLRVLIED
HHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
KDQKSAQKLCEKFASQFRNIKQVELVGYGICGVEMLYGKYRFYLLLRSENHKALVAIENY
CCHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHCCEEEEEEEECCCCCEEEEEEHH
ILQFKNASADIDPIDFV
HHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQRSDIRYYELAICGLYLDNLTFHSFDEIKPLTQVLVDLKSKKNLKAIVLKECQKPDFKT
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCE
IEIKEITGYFLTPLQFELANFIVYYYASKLGFVLGFFETSPKYECHKMFFKDTPKLSNQQ
EEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHCCCCCCCCCH
QNALSFLQKENNSLLFADTGSGKTEIYISLIKECLEQGKQALLLMPEIALTPQMQKRLEV
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCHHCCHHHHHHEEE
YFKDNFFLWHSKISKKKKQEYLERFCKGEVLLVAGARSALFLPFRNLGLIVVDEEHDNSY
EECCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCEEEEECCCCCCC
KASNQPFINARDLALFLGQKNNIKVVLGSATPSLTSFYKQKSFRLKGTFFESKKHFLYDE
CCCCCCCCCHHHEEEEECCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCCEEECC
NELGITPMLLSELEKSLKHQKQAIVFLPTRANFRQIICKDCGETIKCPFCSIAMSMHKKK
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCC
NVLKCHYCNYTSLIEQNCPSCKGEMLEARKMGTAELLELLQNALPLAKIAKFDSDEITSV
CCEEEEECCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCHHHHHH
KKLNTILKDFNENKIDILIGTSMLAKGHDYHSVDLSVILGLDEYLLRPSFRASEETLALA
HHHHHHHHHCCCCEEEEEEECHHHHCCCCCCEEEEEEEECCHHHHHCCCCCCCHHHHHHH
MQVAGRAGRKGEARVLLQTKNRAFFERYIENYDAFLKDELENRKDLYPPFKRLLRVLIED
HHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
KDQKSAQKLCEKFASQFRNIKQVELVGYGICGVEMLYGKYRFYLLLRSENHKALVAIENY
CCHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHCCEEEEEEEECCCCCEEEEEEHH
ILQFKNASADIDPIDFV
HHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]