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The map label for this gene is rsmF [H]

Identifier: 57236949

GI number: 57236949

Start: 674835

End: 675674

Strand: Direct

Name: rsmF [H]

Synonym: CJE0739

Alternate gene names: 57236949

Gene position: 674835-675674 (Clockwise)

Preceding gene: 57236948

Following gene: 57236954

Centisome position: 37.96

GC content: 27.86

Gene sequence:

>840_bases
ATGCAAAATATTTTATCTTCTTTTACTCAAGAAAAAAATGTTTGTGTATTTACAAATACTTTAAAAATTAGCATTGAAGA
ATTAGAAAAAGAATTTTTAAAACAAAAATTAAAATTTAAAAAGATCAATGCTTATTGTTATTTGTTTGATGCCAAAGATA
AGGCTATCTTAAGTTCTATGAAAGTTTTTAATGAGGCGCACTTTTATATACAAAATTATTCTTCTTATTTGTGTGCTTTA
AATTTAGAAGTCAAAGCAGGGCAAAGGGTTCTTGATATGTGTGCAGCTCCGGGTGGTAAAAGTATCAATCTTGCAAATTT
TATGCAAAATACAGGTTATTTAGCTTGCAATGAGATGAGTAGGGATAGATTTTTTATTTTGCAAAAAAATCTTAAAAATT
ACGGCGTGAATGCTAAAGTTTTTATGAAAGATGGTAAAAATATAGGCAATTTATGTCCTTTAAAATTTGATAAAATCTTA
CTTGATGCACCTTGTTCGACTTTTGCTAAAATAGGTTTTGATTTGGAAAAAAGTTATAAAGAAATCAAAAATATTGCAAA
AACTCAAAAAAAACTTCTACATTCTGCTTTAAAGGCATTAAAAATAGGTGGAGAGCTTGTATATAGTACTTGCACCTTTA
CCAAAGAAGAAAATGAAGAAGTGATAGAGAATGCTTTAAAAAGTGAGTTTAAATTAGAGCTTTTAGATATTGATTTGGAA
AATGTTGAGGCTAAAGCAGGGCAAAGTGAAGAATTTGCTGAAATTTCAAAATGCAGACGGATTTTACCAAGCTTAGACTA
TGATGGATTTTTCATAGCTAAACTTAGGAAATTATGTTAA

Upstream 100 bases:

>100_bases
ACACGCAAAAAAGATGGTAAATTAGACTCCTTATCCGCTTTTATCATGATAAAGGATTATTTTGCTCTCTAGAATAGAAA
AACTTTATACCAAAGATGAA

Downstream 100 bases:

>100_bases
TTAATAAAATTATTCACATTTAAATACAAATTCATAAAAAAACTTAAAAAACAATAAATAATTTTTTCTTTATTTTAAAG
CTTAAAGTTCTATTTTTAAG

Product: NOL1/NOP2/sun family protein

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MQNILSSFTQEKNVCVFTNTLKISIEELEKEFLKQKLKFKKINAYCYLFDAKDKAILSSMKVFNEAHFYIQNYSSYLCAL
NLEVKAGQRVLDMCAAPGGKSINLANFMQNTGYLACNEMSRDRFFILQKNLKNYGVNAKVFMKDGKNIGNLCPLKFDKIL
LDAPCSTFAKIGFDLEKSYKEIKNIAKTQKKLLHSALKALKIGGELVYSTCTFTKEENEEVIENALKSEFKLELLDIDLE
NVEAKAGQSEEFAEISKCRRILPSLDYDGFFIAKLRKLC

Sequences:

>Translated_279_residues
MQNILSSFTQEKNVCVFTNTLKISIEELEKEFLKQKLKFKKINAYCYLFDAKDKAILSSMKVFNEAHFYIQNYSSYLCAL
NLEVKAGQRVLDMCAAPGGKSINLANFMQNTGYLACNEMSRDRFFILQKNLKNYGVNAKVFMKDGKNIGNLCPLKFDKIL
LDAPCSTFAKIGFDLEKSYKEIKNIAKTQKKLLHSALKALKIGGELVYSTCTFTKEENEEVIENALKSEFKLELLDIDLE
NVEAKAGQSEEFAEISKCRRILPSLDYDGFFIAKLRKLC
>Mature_279_residues
MQNILSSFTQEKNVCVFTNTLKISIEELEKEFLKQKLKFKKINAYCYLFDAKDKAILSSMKVFNEAHFYIQNYSSYLCAL
NLEVKAGQRVLDMCAAPGGKSINLANFMQNTGYLACNEMSRDRFFILQKNLKNYGVNAKVFMKDGKNIGNLCPLKFDKIL
LDAPCSTFAKIGFDLEKSYKEIKNIAKTQKKLLHSALKALKIGGELVYSTCTFTKEENEEVIENALKSEFKLELLDIDLE
NVEAKAGQSEEFAEISKCRRILPSLDYDGFFIAKLRKLC

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI11545785, Length=246, Percent_Identity=35.3658536585366, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI76150625, Length=293, Percent_Identity=32.4232081911263, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI76150623, Length=293, Percent_Identity=32.4232081911263, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI40316918, Length=209, Percent_Identity=32.5358851674641, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI32698918, Length=174, Percent_Identity=34.4827586206897, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI270288818, Length=232, Percent_Identity=27.1551724137931, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI23199998, Length=232, Percent_Identity=27.1551724137931, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI8922322, Length=232, Percent_Identity=27.1551724137931, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI270288816, Length=232, Percent_Identity=27.1551724137931, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI39995082, Length=191, Percent_Identity=29.3193717277487, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI301336155, Length=191, Percent_Identity=29.3193717277487, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87081985, Length=277, Percent_Identity=36.101083032491, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI2367212, Length=229, Percent_Identity=30.5676855895196, Blast_Score=101, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17536757, Length=292, Percent_Identity=30.1369863013699, Blast_Score=120, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17509941, Length=165, Percent_Identity=29.6969696969697, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324268, Length=284, Percent_Identity=31.3380281690141, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319447, Length=178, Percent_Identity=31.4606741573034, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI22024126, Length=285, Percent_Identity=32.280701754386, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24668781, Length=234, Percent_Identity=32.4786324786325, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356513, Length=234, Percent_Identity=32.4786324786325, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920494, Length=180, Percent_Identity=32.7777777777778, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355201, Length=313, Percent_Identity=26.8370607028754, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21356579, Length=241, Percent_Identity=30.7053941908714, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 31821; Mature: 31821

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNILSSFTQEKNVCVFTNTLKISIEELEKEFLKQKLKFKKINAYCYLFDAKDKAILSSM
CCHHHHHHHCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHH
KVFNEAHFYIQNYSSYLCALNLEVKAGQRVLDMCAAPGGKSINLANFMQNTGYLACNEMS
HHHHHHHHHHHCCCCEEEEEEEEHHCCHHHHHHHCCCCCCCEEHHHHHHCCCCEEECCCC
RDRFFILQKNLKNYGVNAKVFMKDGKNIGNLCPLKFDKILLDAPCSTFAKIGFDLEKSYK
CCEEEEEEHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHEECCCHHHHHHHCCCHHHHHH
EIKNIAKTQKKLLHSALKALKIGGELVYSTCTFTKEENEEVIENALKSEFKLELLDIDLE
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEEEEECCHH
NVEAKAGQSEEFAEISKCRRILPSLDYDGFFIAKLRKLC
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
>Mature Secondary Structure
MQNILSSFTQEKNVCVFTNTLKISIEELEKEFLKQKLKFKKINAYCYLFDAKDKAILSSM
CCHHHHHHHCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHHHH
KVFNEAHFYIQNYSSYLCALNLEVKAGQRVLDMCAAPGGKSINLANFMQNTGYLACNEMS
HHHHHHHHHHHCCCCEEEEEEEEHHCCHHHHHHHCCCCCCCEEHHHHHHCCCCEEECCCC
RDRFFILQKNLKNYGVNAKVFMKDGKNIGNLCPLKFDKILLDAPCSTFAKIGFDLEKSYK
CCEEEEEEHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHEECCCHHHHHHHCCCHHHHHH
EIKNIAKTQKKLLHSALKALKIGGELVYSTCTFTKEENEEVIENALKSEFKLELLDIDLE
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEEEEECCHH
NVEAKAGQSEEFAEISKCRRILPSLDYDGFFIAKLRKLC
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA