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The map label for this gene is pnk [H]

Identifier: 57236954

GI number: 57236954

Start: 679334

End: 680194

Strand: Direct

Name: pnk [H]

Synonym: CJE0744

Alternate gene names: 57236954

Gene position: 679334-680194 (Clockwise)

Preceding gene: 57236949

Following gene: 57236955

Centisome position: 38.21

GC content: 28.46

Gene sequence:

>861_bases
ATGCAAAATAAAATAGATTATAAAAATATTAAAAAAATTGGACTTGTTACGCGACCCAATGTGAGTTTAGATAAGGAAAT
TTTAAAACTTCAATCGATTTTATCTATATATAAAGTTGAACTTGTATTGCTTAAAGAAAGTTCTGAAATTTTAGATTTGC
CAAAGTATGGATTGGATGATTTGTTTAAAATATCCGATTTTGTTATTTCTTTAGGTGGAGATGGAACTTTGATTTCTTTA
TGCCGTAAAGCTTGTGAATATGATAAAGCAGTTTTAGGAATTCATGCTGGACATTTAGGTTTTTTAACAGATTTTAAAGT
TGATGAAGCTGAAAATTTTTTTCAAGCTTTTTTTCAAGGAGAATTTAGAATAGAAAAGCCTTATTTGTTAAGTGTTTTTT
TAGAAGATAAGCAAGGAAAAATTTTAGAAAAATTAGCTTTTAACGATGTGGTCATCAGTAAAAACAATCAAGCTTCTATG
GCTCATATAGAAGTTTTTAGAAAAGAAAAGAAATTTAATGAATATTTTGGAGATGGGCTTATTGTTGCAACTCCTGCGGG
TTCAACAGCTTATAATTTAAGTGCAAATGGGCCCATAGTTTATACTTTAGCTCAAGCTTTTATCTTAACTCCTGTTTGTT
CTCATTCTTTAACACAACGCCCTATTGTCTTACCAAAAGGTTTTGAAATAGAGATTATGGCAAAGGATTGTATACTTTGC
ATTGATGGGCAAGAAAATTATAAAATGAATGATTTTAAAAGTATAAAAGTGGGTTTAAGTGATAAAAATGTGGCTTTAAT
TCACCCAAAAAATAGGGATTATTTTCAAATTTTAAAAGAAAAATTACACTGGGGAAATTGA

Upstream 100 bases:

>100_bases
GACTTCGCAAATTTATTCCTTGAAAAAGATTTTTTAAGATAATGAGCAAAATTATAGTATTTTTATTCTTTTGCTTTGCT
AAGATTTGATATAATTTTTT

Downstream 100 bases:

>100_bases
TGATAAATAGAATTTTTATGAAAGAAAATCTTGGTTTTAAAAAGGCTGAACTTGAAATTTCTAAAGGGCTTACCGTTTTT
ACGGGGCTAAGTGGAGCTGG

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MQNKIDYKNIKKIGLVTRPNVSLDKEILKLQSILSIYKVELVLLKESSEILDLPKYGLDDLFKISDFVISLGGDGTLISL
CRKACEYDKAVLGIHAGHLGFLTDFKVDEAENFFQAFFQGEFRIEKPYLLSVFLEDKQGKILEKLAFNDVVISKNNQASM
AHIEVFRKEKKFNEYFGDGLIVATPAGSTAYNLSANGPIVYTLAQAFILTPVCSHSLTQRPIVLPKGFEIEIMAKDCILC
IDGQENYKMNDFKSIKVGLSDKNVALIHPKNRDYFQILKEKLHWGN

Sequences:

>Translated_286_residues
MQNKIDYKNIKKIGLVTRPNVSLDKEILKLQSILSIYKVELVLLKESSEILDLPKYGLDDLFKISDFVISLGGDGTLISL
CRKACEYDKAVLGIHAGHLGFLTDFKVDEAENFFQAFFQGEFRIEKPYLLSVFLEDKQGKILEKLAFNDVVISKNNQASM
AHIEVFRKEKKFNEYFGDGLIVATPAGSTAYNLSANGPIVYTLAQAFILTPVCSHSLTQRPIVLPKGFEIEIMAKDCILC
IDGQENYKMNDFKSIKVGLSDKNVALIHPKNRDYFQILKEKLHWGN
>Mature_286_residues
MQNKIDYKNIKKIGLVTRPNVSLDKEILKLQSILSIYKVELVLLKESSEILDLPKYGLDDLFKISDFVISLGGDGTLISL
CRKACEYDKAVLGIHAGHLGFLTDFKVDEAENFFQAFFQGEFRIEKPYLLSVFLEDKQGKILEKLAFNDVVISKNNQASM
AHIEVFRKEKKFNEYFGDGLIVATPAGSTAYNLSANGPIVYTLAQAFILTPVCSHSLTQRPIVLPKGFEIEIMAKDCILC
IDGQENYKMNDFKSIKVGLSDKNVALIHPKNRDYFQILKEKLHWGN

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=259, Percent_Identity=28.1853281853282, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1788968, Length=233, Percent_Identity=34.3347639484979, Blast_Score=134, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6320794, Length=241, Percent_Identity=26.9709543568465, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6325068, Length=176, Percent_Identity=32.3863636363636, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6322509, Length=239, Percent_Identity=25.9414225941423, Blast_Score=92, Evalue=9e-20,
Organism=Drosophila melanogaster, GI161077047, Length=255, Percent_Identity=31.3725490196078, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28573832, Length=256, Percent_Identity=31.25, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28573830, Length=255, Percent_Identity=31.3725490196078, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28573826, Length=255, Percent_Identity=31.3725490196078, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28573828, Length=255, Percent_Identity=31.3725490196078, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI20129957, Length=317, Percent_Identity=29.0220820189274, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653422, Length=317, Percent_Identity=29.0220820189274, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653424, Length=317, Percent_Identity=29.0220820189274, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281363323, Length=317, Percent_Identity=29.0220820189274, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281363321, Length=317, Percent_Identity=29.0220820189274, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 32322; Mature: 32322

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKIDYKNIKKIGLVTRPNVSLDKEILKLQSILSIYKVELVLLKESSEILDLPKYGLDD
CCCCCCHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCHHH
LFKISDFVISLGGDGTLISLCRKACEYDKAVLGIHAGHLGFLTDFKVDEAENFFQAFFQG
HHHHHHEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHHHHHHHHCC
EFRIEKPYLLSVFLEDKQGKILEKLAFNDVVISKNNQASMAHIEVFRKEKKFNEYFGDGL
CEEECCCEEEEEEEECCCCHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCE
IVATPAGSTAYNLSANGPIVYTLAQAFILTPVCSHSLTQRPIVLPKGFEIEIMAKDCILC
EEEECCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEECCEEEE
IDGQENYKMNDFKSIKVGLSDKNVALIHPKNRDYFQILKEKLHWGN
EECCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MQNKIDYKNIKKIGLVTRPNVSLDKEILKLQSILSIYKVELVLLKESSEILDLPKYGLDD
CCCCCCHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCHHH
LFKISDFVISLGGDGTLISLCRKACEYDKAVLGIHAGHLGFLTDFKVDEAENFFQAFFQG
HHHHHHEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCHHHHHHHHHHCC
EFRIEKPYLLSVFLEDKQGKILEKLAFNDVVISKNNQASMAHIEVFRKEKKFNEYFGDGL
CEEECCCEEEEEEEECCCCHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCE
IVATPAGSTAYNLSANGPIVYTLAQAFILTPVCSHSLTQRPIVLPKGFEIEIMAKDCILC
EEEECCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEEEECCEEEE
IDGQENYKMNDFKSIKVGLSDKNVALIHPKNRDYFQILKEKLHWGN
EECCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA