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The map label for this gene is leuC [H]

Identifier: 57236904

GI number: 57236904

Start: 1764931

End: 1766337

Strand: Reverse

Name: leuC [H]

Synonym: CJE1887

Alternate gene names: 57236904

Gene position: 1766337-1764931 (Counterclockwise)

Preceding gene: 57236905

Following gene: 57236903

Centisome position: 99.35

GC content: 36.03

Gene sequence:

>1407_bases
ATGGCTAAAACTTTATATGAGAAAGTCTTTGATGCTCATGTGGTTTATGAAGGCAAAAACGAACTTCCTATACTTTATAT
CGATAGACATTTAATCCATGAAGTAACAAGCCCTCAAGCATTTTCAGGGCTTAAAATGGCTAAGCGTAGAATGGCAAGAG
CGGATTTAACTTTGGCTACCATAGATCATGATGTTTCAACTAAAAGCGTGGATTTAAATGCTTGTTCTGATATGGCAAAA
GAGCAAATTACAACTTTGATGCAAAATACTAAGGAATTTGGAGTAAGGCTTTTAGGACTTGGAGATAAAAATCAAGGCAT
AGTTCATATAGTAGGTCCTGAGCTTGGCTTTACTTTACCTGGGGTTACTTTAGTTTGTGGGGATTCTCATACGGCAACAC
ATGGGGCTTTTGGAGCTTTAGCCTTTGGTATAGGCACGAGTGAAGTAGAGCATGTCATGGCAACGCAAACTTTAAAACAA
GCCAAACTTAAAACTATGAAAATCGAATGTAAAGGGCAGTTTCAAAAAGGCGTTTATACTAAGGATTTGATTTTGTATTT
GATCGCACAATACGGCACAGCAAAAGGCACAGGCTATGCTATAGAATTTTGCGGAGAACTTATAAGAAATCTAAGCATGG
AAGCTAGAATGACTCTTTGTAATATGGCTATAGAATTTGGAGCGAAAGTAGGTATGATAGCTCCTGATGAGATCACTTTT
GAGTATATTAAGGGAAAGGAATTTGCACCTAAAGGAGAAGAATTTCAAAAGTATTGTGAGTATTGGAAGAGTTTAAGAAG
TGATGAAGGAGCAAAATATGATGCGAGTATTACTCTAGATGTAAGCAAGATAAAACCACAAATTAGCTATGGAACAAATC
CTTCGCAAGTGATTGGCATAGATGAAAAAATTCCAAAAATAAGTGATTTTAAAAATCAAAGCGAGCAAAAATCTTTGCTT
GATGCTTTATATTATGTAAATTTAGAGCAAGATCAAGTTATAGAAGGGGTAAAAATCGACATTGTATTTATAGGATCTTG
CACAAATGGGCGTTTAGAAGATCTTAAAATAGCTGCTGATATCTTAAAAGGGCGCAAAATTCATAAAAATGTTAAAGCTT
TAATCGTGCCTGGTTCAATGCAAGTAAGAAAAGAAGCTGAAAATTTAGGACTTGATAAGATTTTTATCGAGGCAGGGTGT
GAGTGGAGATATGCAGGGTGTTCTATGTGTCTTGGTATGAATGATGACAAAGCAAATTCAGGTCAAAGAGTTGCTTCAAC
TTCAAATCGTAACTTTGTAGGCAGACAAGGTAAAGGTTCTATCACTCATTTGATGAGTCCTGCAAGTGCGGCAGCTTGTG
CCATAGAAGGTGTTATTTGTGATAATAGAAAGTATTTAGGAGTTTAA

Upstream 100 bases:

>100_bases
TTGGCACAAGGAAAAATGACTAAGGATTTAAATGCTAAGTCGTATTTAAATACTGATGAAATGGGGGATTGTATTTTAGA
AATTTTAAAGGAGAATAATA

Downstream 100 bases:

>100_bases
TATGCAAAAATTTATTATACACAAAGGTATAGCTTGTCCTTTAGAATATGCCAATATAGACACAGATCAAATTATACCTA
AGCAATTTTTACTTGCAGTG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MAKTLYEKVFDAHVVYEGKNELPILYIDRHLIHEVTSPQAFSGLKMAKRRMARADLTLATIDHDVSTKSVDLNACSDMAK
EQITTLMQNTKEFGVRLLGLGDKNQGIVHIVGPELGFTLPGVTLVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLKQ
AKLKTMKIECKGQFQKGVYTKDLILYLIAQYGTAKGTGYAIEFCGELIRNLSMEARMTLCNMAIEFGAKVGMIAPDEITF
EYIKGKEFAPKGEEFQKYCEYWKSLRSDEGAKYDASITLDVSKIKPQISYGTNPSQVIGIDEKIPKISDFKNQSEQKSLL
DALYYVNLEQDQVIEGVKIDIVFIGSCTNGRLEDLKIAADILKGRKIHKNVKALIVPGSMQVRKEAENLGLDKIFIEAGC
EWRYAGCSMCLGMNDDKANSGQRVASTSNRNFVGRQGKGSITHLMSPASAAACAIEGVICDNRKYLGV

Sequences:

>Translated_468_residues
MAKTLYEKVFDAHVVYEGKNELPILYIDRHLIHEVTSPQAFSGLKMAKRRMARADLTLATIDHDVSTKSVDLNACSDMAK
EQITTLMQNTKEFGVRLLGLGDKNQGIVHIVGPELGFTLPGVTLVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLKQ
AKLKTMKIECKGQFQKGVYTKDLILYLIAQYGTAKGTGYAIEFCGELIRNLSMEARMTLCNMAIEFGAKVGMIAPDEITF
EYIKGKEFAPKGEEFQKYCEYWKSLRSDEGAKYDASITLDVSKIKPQISYGTNPSQVIGIDEKIPKISDFKNQSEQKSLL
DALYYVNLEQDQVIEGVKIDIVFIGSCTNGRLEDLKIAADILKGRKIHKNVKALIVPGSMQVRKEAENLGLDKIFIEAGC
EWRYAGCSMCLGMNDDKANSGQRVASTSNRNFVGRQGKGSITHLMSPASAAACAIEGVICDNRKYLGV
>Mature_467_residues
AKTLYEKVFDAHVVYEGKNELPILYIDRHLIHEVTSPQAFSGLKMAKRRMARADLTLATIDHDVSTKSVDLNACSDMAKE
QITTLMQNTKEFGVRLLGLGDKNQGIVHIVGPELGFTLPGVTLVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLKQA
KLKTMKIECKGQFQKGVYTKDLILYLIAQYGTAKGTGYAIEFCGELIRNLSMEARMTLCNMAIEFGAKVGMIAPDEITFE
YIKGKEFAPKGEEFQKYCEYWKSLRSDEGAKYDASITLDVSKIKPQISYGTNPSQVIGIDEKIPKISDFKNQSEQKSLLD
ALYYVNLEQDQVIEGVKIDIVFIGSCTNGRLEDLKIAADILKGRKIHKNVKALIVPGSMQVRKEAENLGLDKIFIEAGCE
WRYAGCSMCLGMNDDKANSGQRVASTSNRNFVGRQGKGSITHLMSPASAAACAIEGVICDNRKYLGV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=402, Percent_Identity=27.363184079602, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI41352693, Length=370, Percent_Identity=24.3243243243243, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI8659555, Length=460, Percent_Identity=24.3478260869565, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=60.475161987041, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI87081781, Length=350, Percent_Identity=28.2857142857143, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787531, Length=360, Percent_Identity=25.8333333333333, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=398, Percent_Identity=29.1457286432161, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=396, Percent_Identity=29.5454545454545, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=28.8524590163934, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=458, Percent_Identity=25.5458515283843, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=55.4140127388535, Blast_Score=525, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6320440, Length=422, Percent_Identity=26.0663507109005, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=397, Percent_Identity=26.448362720403, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=396, Percent_Identity=27.020202020202, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI281365315, Length=477, Percent_Identity=26.4150943396226, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17864292, Length=477, Percent_Identity=26.4150943396226, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161076999, Length=395, Percent_Identity=26.8354430379747, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28571643, Length=477, Percent_Identity=27.2536687631027, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24645686, Length=463, Percent_Identity=25.7019438444924, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137564, Length=369, Percent_Identity=26.2872628726287, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51447; Mature: 51316

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYEKVFDAHVVYEGKNELPILYIDRHLIHEVTSPQAFSGLKMAKRRMARADLTLAT
CCHHHHHHHHCCEEEEECCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHCEEEEE
IDHDVSTKSVDLNACSDMAKEQITTLMQNTKEFGVRLLGLGDKNQGIVHIVGPELGFTLP
ECCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHCEEEEECCCCCCCEEEEECCCCCCCCC
GVTLVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLKQAKLKTMKIECKGQFQKGVYT
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHH
KDLILYLIAQYGTAKGTGYAIEFCGELIRNLSMEARMTLCNMAIEFGAKVGMIAPDEITF
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEE
EYIKGKEFAPKGEEFQKYCEYWKSLRSDEGAKYDASITLDVSKIKPQISYGTNPSQVIGI
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEECEEEEEEHHHCCCEEECCCCCCEEEEC
DEKIPKISDFKNQSEQKSLLDALYYVNLEQDQVIEGVKIDIVFIGSCTNGRLEDLKIAAD
HHCCCCCHHHCCCHHHHHHHHHHHEECCCHHHHCCCEEEEEEEEECCCCCCHHHHHHHHH
ILKGRKIHKNVKALIVPGSMQVRKEAENLGLDKIFIEAGCEWRYAGCSMCLGMNDDKANS
HHCCCHHHCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCEEECCEEEEECCCCCCCCC
GQRVASTSNRNFVGRQGKGSITHLMSPASAAACAIEGVICDNRKYLGV
CCEEECCCCCCCCCCCCCCCHHHHCCCCHHHHHEEEEEEECCCEECCC
>Mature Secondary Structure 
AKTLYEKVFDAHVVYEGKNELPILYIDRHLIHEVTSPQAFSGLKMAKRRMARADLTLAT
CHHHHHHHHCCEEEEECCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHCEEEEE
IDHDVSTKSVDLNACSDMAKEQITTLMQNTKEFGVRLLGLGDKNQGIVHIVGPELGFTLP
ECCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHCEEEEECCCCCCCEEEEECCCCCCCCC
GVTLVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLKQAKLKTMKIECKGQFQKGVYT
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHH
KDLILYLIAQYGTAKGTGYAIEFCGELIRNLSMEARMTLCNMAIEFGAKVGMIAPDEITF
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEE
EYIKGKEFAPKGEEFQKYCEYWKSLRSDEGAKYDASITLDVSKIKPQISYGTNPSQVIGI
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEECEEEEEEHHHCCCEEECCCCCCEEEEC
DEKIPKISDFKNQSEQKSLLDALYYVNLEQDQVIEGVKIDIVFIGSCTNGRLEDLKIAAD
HHCCCCCHHHCCCHHHHHHHHHHHEECCCHHHHCCCEEEEEEEEECCCCCCHHHHHHHHH
ILKGRKIHKNVKALIVPGSMQVRKEAENLGLDKIFIEAGCEWRYAGCSMCLGMNDDKANS
HHCCCHHHCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCEEECCEEEEECCCCCCCCC
GQRVASTSNRNFVGRQGKGSITHLMSPASAAACAIEGVICDNRKYLGV
CCEEECCCCCCCCCCCCCCCHHHHCCCCHHHHHEEEEEEECCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]