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The map label for this gene is leuB [H]

Identifier: 57236905

GI number: 57236905

Start: 1766330

End: 1767406

Strand: Reverse

Name: leuB [H]

Synonym: CJE1888

Alternate gene names: 57236905

Gene position: 1767406-1766330 (Counterclockwise)

Preceding gene: 57236906

Following gene: 57236904

Centisome position: 99.41

GC content: 33.61

Gene sequence:

>1077_bases
ATGAAAGCTTATAAAGTAGCTGTTTTAGCAGGAGATGGCATAGGGCCTTTGGTGATGAAAGAAGCTTTAAAAATTTTAAC
TTTTATCTCACAAAAATACAATTTTAGTTTTGAATTTAATGAAGCTAAAATAGGCGGTGCAAGTATAGATGCTTATGGAG
TGGCTTTAAGTGATGAAACTTTAAAGCTTTGTGAGCAAAGTGATGCGATTTTATTTGGCTCTGTGGGTGGGCCTAAGTGG
GATAATTTGCCCATAGATCAAAGACCTGAAAGAGCCTCGCTTTTGCCTTTAAGAAAGCATTTTAATTTGTTTGCAAATTT
GCGTCCTTGTAAAATTTATGAAAGTTTAACTCATGCTTCACCTTTAAAAAATGAGATTATCCAAAAAGGAGTGGATATTT
TATGTGTTAGAGAGCTTACAGGTGGGATTTATTTTGGTAAGCAAGATTTAGGTAAAGAAAGTGCGTATGATACTGAAATT
TACACTAAAAAAGAGATAGAAAGGATAGCTCATATCGCTTTTGAAAGTGCAAGAATAAGAAAGAAAAAAGTGCATTTAAT
CGACAAGGCGAATGTTTTAGCAAGTTCTATTTTGTGGCGAGAAGTGGTGGCAAATGTTGTAAAAGATTATCAAGATATAA
ACTTAGAGTATATGTATGTGGATAATGCTGCGATGCAGATTGTAAAAAATCCTAGCATTTTTGATGTTATGCTTTGTTCA
AATCTTTTTGGTGATATTTTGAGTGATGAGTTAGCTGCCATTAATGGATCTTTGGGTTTGTTAAGTTCAGCAAGTTTAAA
CGATAAAGGTTTTGGACTTTATGAGCCAGCAGGTGGAAGTGCGCCTGATATAGCTCATTTAAATATAGCTAATCCCATAG
CTCAAATTTTAAGTGCGGCTTTGATGTTAAAGTATAGTTTCAAAGAAGAACAAGCCGCACAGGATATAGAAAATGCTATA
TCTTTGGCATTGGCACAAGGAAAAATGACTAAGGATTTAAATGCTAAGTCGTATTTAAATACTGATGAAATGGGGGATTG
TATTTTAGAAATTTTAAAGGAGAATAATAATGGCTAA

Upstream 100 bases:

>100_bases
AAGGACTTTCAACAGATGTTATAGAAGCTTCAGCACAAGCTTTTGCGAGTGCTTACAATGCGATTTATCGCTCTTTAAAA
GTAGAAGAAAGGAAAATGGC

Downstream 100 bases:

>100_bases
AACTTTATATGAGAAAGTCTTTGATGCTCATGTGGTTTATGAAGGCAAAAACGAACTTCCTATACTTTATATCGATAGAC
ATTTAATCCATGAAGTAACA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKAYKVAVLAGDGIGPLVMKEALKILTFISQKYNFSFEFNEAKIGGASIDAYGVALSDETLKLCEQSDAILFGSVGGPKW
DNLPIDQRPERASLLPLRKHFNLFANLRPCKIYESLTHASPLKNEIIQKGVDILCVRELTGGIYFGKQDLGKESAYDTEI
YTKKEIERIAHIAFESARIRKKKVHLIDKANVLASSILWREVVANVVKDYQDINLEYMYVDNAAMQIVKNPSIFDVMLCS
NLFGDILSDELAAINGSLGLLSSASLNDKGFGLYEPAGGSAPDIAHLNIANPIAQILSAALMLKYSFKEEQAAQDIENAI
SLALAQGKMTKDLNAKSYLNTDEMGDCILEILKENNNG

Sequences:

>Translated_358_residues
MKAYKVAVLAGDGIGPLVMKEALKILTFISQKYNFSFEFNEAKIGGASIDAYGVALSDETLKLCEQSDAILFGSVGGPKW
DNLPIDQRPERASLLPLRKHFNLFANLRPCKIYESLTHASPLKNEIIQKGVDILCVRELTGGIYFGKQDLGKESAYDTEI
YTKKEIERIAHIAFESARIRKKKVHLIDKANVLASSILWREVVANVVKDYQDINLEYMYVDNAAMQIVKNPSIFDVMLCS
NLFGDILSDELAAINGSLGLLSSASLNDKGFGLYEPAGGSAPDIAHLNIANPIAQILSAALMLKYSFKEEQAAQDIENAI
SLALAQGKMTKDLNAKSYLNTDEMGDCILEILKENNNG
>Mature_358_residues
MKAYKVAVLAGDGIGPLVMKEALKILTFISQKYNFSFEFNEAKIGGASIDAYGVALSDETLKLCEQSDAILFGSVGGPKW
DNLPIDQRPERASLLPLRKHFNLFANLRPCKIYESLTHASPLKNEIIQKGVDILCVRELTGGIYFGKQDLGKESAYDTEI
YTKKEIERIAHIAFESARIRKKKVHLIDKANVLASSILWREVVANVVKDYQDINLEYMYVDNAAMQIVKNPSIFDVMLCS
NLFGDILSDELAAINGSLGLLSSASLNDKGFGLYEPAGGSAPDIAHLNIANPIAQILSAALMLKYSFKEEQAAQDIENAI
SLALAQGKMTKDLNAKSYLNTDEMGDCILEILKENNNG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=345, Percent_Identity=32.7536231884058, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI28178821, Length=313, Percent_Identity=28.1150159744409, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI28178838, Length=321, Percent_Identity=28.9719626168224, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI4758582, Length=353, Percent_Identity=28.0453257790368, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI28178816, Length=313, Percent_Identity=28.1150159744409, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI28178819, Length=151, Percent_Identity=33.7748344370861, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI87081683, Length=361, Percent_Identity=59.2797783933518, Blast_Score=430, Evalue=1e-122,
Organism=Escherichia coli, GI1788101, Length=369, Percent_Identity=34.6883468834688, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1787381, Length=399, Percent_Identity=24.5614035087719, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=342, Percent_Identity=31.2865497076023, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17505779, Length=306, Percent_Identity=31.3725490196078, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=311, Percent_Identity=29.2604501607717, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17550882, Length=355, Percent_Identity=27.0422535211268, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319830, Length=363, Percent_Identity=45.1790633608815, Blast_Score=273, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6322097, Length=371, Percent_Identity=33.9622641509434, Blast_Score=156, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6324709, Length=356, Percent_Identity=30.3370786516854, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=340, Percent_Identity=27.6470588235294, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24643268, Length=340, Percent_Identity=31.7647058823529, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24643270, Length=340, Percent_Identity=31.7647058823529, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24661184, Length=359, Percent_Identity=28.133704735376, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078635, Length=355, Percent_Identity=28.4507042253521, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI161078637, Length=355, Percent_Identity=28.4507042253521, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078633, Length=355, Percent_Identity=28.169014084507, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24650122, Length=355, Percent_Identity=28.169014084507, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078639, Length=261, Percent_Identity=31.8007662835249, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI281362242, Length=337, Percent_Identity=26.7062314540059, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648872, Length=337, Percent_Identity=26.7062314540059, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20130355, Length=317, Percent_Identity=24.9211356466877, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39363; Mature: 39363

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAYKVAVLAGDGIGPLVMKEALKILTFISQKYNFSFEFNEAKIGGASIDAYGVALSDET
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECHHH
LKLCEQSDAILFGSVGGPKWDNLPIDQRPERASLLPLRKHFNLFANLRPCKIYESLTHAS
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
PLKNEIIQKGVDILCVRELTGGIYFGKQDLGKESAYDTEIYTKKEIERIAHIAFESARIR
HHHHHHHHCCCCEEEEEHHHCCEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KKKVHLIDKANVLASSILWREVVANVVKDYQDINLEYMYVDNAAMQIVKNPSIFDVMLCS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHCCCCHHHHHHHH
NLFGDILSDELAAINGSLGLLSSASLNDKGFGLYEPAGGSAPDIAHLNIANPIAQILSAA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEECHHHHHHHHHHHH
LMLKYSFKEEQAAQDIENAISLALAQGKMTKDLNAKSYLNTDEMGDCILEILKENNNG
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKAYKVAVLAGDGIGPLVMKEALKILTFISQKYNFSFEFNEAKIGGASIDAYGVALSDET
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECHHH
LKLCEQSDAILFGSVGGPKWDNLPIDQRPERASLLPLRKHFNLFANLRPCKIYESLTHAS
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
PLKNEIIQKGVDILCVRELTGGIYFGKQDLGKESAYDTEIYTKKEIERIAHIAFESARIR
HHHHHHHHCCCCEEEEEHHHCCEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KKKVHLIDKANVLASSILWREVVANVVKDYQDINLEYMYVDNAAMQIVKNPSIFDVMLCS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHCCCCHHHHHHHH
NLFGDILSDELAAINGSLGLLSSASLNDKGFGLYEPAGGSAPDIAHLNIANPIAQILSAA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEECHHHHHHHHHHHH
LMLKYSFKEEQAAQDIENAISLALAQGKMTKDLNAKSYLNTDEMGDCILEILKENNNG
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]