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The map label for this gene is leuD [H]

Identifier: 57236903

GI number: 57236903

Start: 1764327

End: 1764929

Strand: Reverse

Name: leuD [H]

Synonym: CJE1886

Alternate gene names: 57236903

Gene position: 1764929-1764327 (Counterclockwise)

Preceding gene: 57236904

Following gene: 57236897

Centisome position: 99.27

GC content: 29.35

Gene sequence:

>603_bases
ATGCAAAAATTTATTATACACAAAGGTATAGCTTGTCCTTTAGAATATGCCAATATAGACACAGATCAAATTATACCTAA
GCAATTTTTACTTGCAGTGTCTAAGCAAGGTTTTGGTAGGCATTTGTTTCATGATTTGCGTTATTTAGATGATAAAGAAA
GTGTTTTAAATATGGATTTTAATCTCAATAAAAAAGAATATCAAAATAGCTCTATTTTGGTGAGTTTTGAAAATTTTGGT
AGTGGCTCTTCAAGAGAGCATGCGCCTTGGGCTTTGGTTGATTATGGTATTAGAGCTATTATCGCACCTTCTTTTGCGGA
TATTTTTAAAAACAATGCCTTAGGAAACGGTTTGCTCACTATAGAACTTGCTAAAGATGAGGTTTTGGAAATAGTTGATG
AGTTAAAAAAATCCCAAGATAAAAATATAGAAATTTCTTTGTTGGAAAAAAGAGTATTTTTTAAAGATAAGATTTTTTCT
TTTGATTTAGATAATTTTCACAGAATTTGTCTTTTGGAAGGACTTGATAATATAGCTTTAACTTTAAAACATGAAGCACA
AATCAAAGCCTATGAAAAAAACTCAAAAAGCTTTTTGGTTTAG

Upstream 100 bases:

>100_bases
GGTAAAGGTTCTATCACTCATTTGATGAGTCCTGCAAGTGCGGCAGCTTGTGCCATAGAAGGTGTTATTTGTGATAATAG
AAAGTATTTAGGAGTTTAAT

Downstream 100 bases:

>100_bases
AATTTTTTCATAAGCTAAAAAAGGATCTTGGTAATTAGGTATGTCAAAGTTTCCACAAAAATTAAAATCAAGCTTTTTAA
AAAGCGAATTCATAGGAAAA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MQKFIIHKGIACPLEYANIDTDQIIPKQFLLAVSKQGFGRHLFHDLRYLDDKESVLNMDFNLNKKEYQNSSILVSFENFG
SGSSREHAPWALVDYGIRAIIAPSFADIFKNNALGNGLLTIELAKDEVLEIVDELKKSQDKNIEISLLEKRVFFKDKIFS
FDLDNFHRICLLEGLDNIALTLKHEAQIKAYEKNSKSFLV

Sequences:

>Translated_200_residues
MQKFIIHKGIACPLEYANIDTDQIIPKQFLLAVSKQGFGRHLFHDLRYLDDKESVLNMDFNLNKKEYQNSSILVSFENFG
SGSSREHAPWALVDYGIRAIIAPSFADIFKNNALGNGLLTIELAKDEVLEIVDELKKSQDKNIEISLLEKRVFFKDKIFS
FDLDNFHRICLLEGLDNIALTLKHEAQIKAYEKNSKSFLV
>Mature_200_residues
MQKFIIHKGIACPLEYANIDTDQIIPKQFLLAVSKQGFGRHLFHDLRYLDDKESVLNMDFNLNKKEYQNSSILVSFENFG
SGSSREHAPWALVDYGIRAIIAPSFADIFKNNALGNGLLTIELAKDEVLEIVDELKKSQDKNIEISLLEKRVFFKDKIFS
FDLDNFHRICLLEGLDNIALTLKHEAQIKAYEKNSKSFLV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=198, Percent_Identity=49.4949494949495, Blast_Score=205, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321429, Length=209, Percent_Identity=42.1052631578947, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22927; Mature: 22927

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKFIIHKGIACPLEYANIDTDQIIPKQFLLAVSKQGFGRHLFHDLRYLDDKESVLNMDF
CCCEEEECCCCCCEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEECCC
NLNKKEYQNSSILVSFENFGSGSSREHAPWALVDYGIRAIIAPSFADIFKNNALGNGLLT
CCCHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHCHHEEECCHHHHHHHCCCCCCCEEE
IELAKDEVLEIVDELKKSQDKNIEISLLEKRVFFKDKIFSFDLDNFHRICLLEGLDNIAL
EEECHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHEEECCCCHHEEHEECCCCCEEE
TLKHEAQIKAYEKNSKSFLV
EEECCCEEEEEECCCCCCCC
>Mature Secondary Structure
MQKFIIHKGIACPLEYANIDTDQIIPKQFLLAVSKQGFGRHLFHDLRYLDDKESVLNMDF
CCCEEEECCCCCCEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEECCC
NLNKKEYQNSSILVSFENFGSGSSREHAPWALVDYGIRAIIAPSFADIFKNNALGNGLLT
CCCHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHCHHEEECCHHHHHHHCCCCCCCEEE
IELAKDEVLEIVDELKKSQDKNIEISLLEKRVFFKDKIFSFDLDNFHRICLLEGLDNIAL
EEECHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHEEECCCCHHEEHEECCCCCEEE
TLKHEAQIKAYEKNSKSFLV
EEECCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA