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The map label for this gene is ksgA [H]

Identifier: 57236897

GI number: 57236897

Start: 1759506

End: 1760306

Strand: Reverse

Name: ksgA [H]

Synonym: CJE1880

Alternate gene names: 57236897

Gene position: 1760306-1759506 (Counterclockwise)

Preceding gene: 57236903

Following gene: 57236896

Centisome position: 99.01

GC content: 29.71

Gene sequence:

>801_bases
ATGGTTAAAGCAAAAAAACAATACGGACAAAATTTTTTAATCGATAAAAGCGTATTAGCAAAAATCATCCAAGCCATACC
CAAAGAGATGAACAATATCATTGAGATTGGGCCTGGCTTAGGTGATTTAACGCAAGAACTTTTAAAAATTTCTCAAGTAA
AAGCTTATGAAATCGATAATGATCTCATACCTATTTTAAAAAAGAAATTTCAAAAAGAACTTGAATGTGGAAAATTTAAC
TTGATCCATCAAGACGCAAGCGAAGCTTTTAATCCTAGTCTTGATGAAAAACCATATTTTTTGGTTGCAAATTTGCCTTA
TTATGTTGCAAGTCACATTATATTAAAAGCTTTAGAAGATAAAAATTGTCTAGGGCTTATTGTAATGGTACAAAGGGAAA
TGGCTGAAAAATTCTGCGCTAAAGAAGGAAATAGTGAGTTTTCTTCCTTAGGTGTTTTAAGTGCAATGATTTGCGAAAGA
AAAATACTTTTTGATGTTGATCCACAATGTTTTAATCCTCCACCAAAAGTTATGTCAGCGGTGATGAGTTTGATTAAAAC
TAAGGATTTTGATGAACTTTGCGAAATAGAAAATTTCAAAAATTTTCTAAAAGATTGTTTTAAAGCTCCTAGAAAACAAC
TCTTAGGAAATTTAAAAACATATAAAGCAAAAGTTTTAGAAGTTTTAAGCACTCTTGGCTTAAAAGAAAACATCAGACCA
CATGAAATTTGCGTTGATTTATACCTTAAAATTTATGACAAATTAAAGGATGAATATGGACGAAAACAAAGAGATAAATA
A

Upstream 100 bases:

>100_bases
CCACCCGCACAAACAATCATTTTGATCCTTATGAAATTTTACAAAAAATAATTCTACCTAAAAAGACTTAATTAAGAATT
TTTAGATAAAATTATTTCTT

Downstream 100 bases:

>100_bases
AAACGAACAAAATCCGAACTCAAATTCAAAAAACAATAAACGCTATAAATATAAAAATCGTAGAAAAAAATTAGCTGATT
CTTTGCAAAATGAAAATGAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MVKAKKQYGQNFLIDKSVLAKIIQAIPKEMNNIIEIGPGLGDLTQELLKISQVKAYEIDNDLIPILKKKFQKELECGKFN
LIHQDASEAFNPSLDEKPYFLVANLPYYVASHIILKALEDKNCLGLIVMVQREMAEKFCAKEGNSEFSSLGVLSAMICER
KILFDVDPQCFNPPPKVMSAVMSLIKTKDFDELCEIENFKNFLKDCFKAPRKQLLGNLKTYKAKVLEVLSTLGLKENIRP
HEICVDLYLKIYDKLKDEYGRKQRDK

Sequences:

>Translated_266_residues
MVKAKKQYGQNFLIDKSVLAKIIQAIPKEMNNIIEIGPGLGDLTQELLKISQVKAYEIDNDLIPILKKKFQKELECGKFN
LIHQDASEAFNPSLDEKPYFLVANLPYYVASHIILKALEDKNCLGLIVMVQREMAEKFCAKEGNSEFSSLGVLSAMICER
KILFDVDPQCFNPPPKVMSAVMSLIKTKDFDELCEIENFKNFLKDCFKAPRKQLLGNLKTYKAKVLEVLSTLGLKENIRP
HEICVDLYLKIYDKLKDEYGRKQRDK
>Mature_266_residues
MVKAKKQYGQNFLIDKSVLAKIIQAIPKEMNNIIEIGPGLGDLTQELLKISQVKAYEIDNDLIPILKKKFQKELECGKFN
LIHQDASEAFNPSLDEKPYFLVANLPYYVASHIILKALEDKNCLGLIVMVQREMAEKFCAKEGNSEFSSLGVLSAMICER
KILFDVDPQCFNPPPKVMSAVMSLIKTKDFDELCEIENFKNFLKDCFKAPRKQLLGNLKTYKAKVLEVLSTLGLKENIRP
HEICVDLYLKIYDKLKDEYGRKQRDK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=204, Percent_Identity=28.921568627451, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI7657198, Length=251, Percent_Identity=27.0916334661355, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1786236, Length=265, Percent_Identity=28.3018867924528, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25141369, Length=291, Percent_Identity=27.1477663230241, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25146882, Length=226, Percent_Identity=29.2035398230088, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21358017, Length=184, Percent_Identity=31.5217391304348, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357273, Length=211, Percent_Identity=27.4881516587678, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30458; Mature: 30458

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS00322 HISTONE_H3_1 ; PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKAKKQYGQNFLIDKSVLAKIIQAIPKEMNNIIEIGPGLGDLTQELLKISQVKAYEIDN
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DLIPILKKKFQKELECGKFNLIHQDASEAFNPSLDEKPYFLVANLPYYVASHIILKALED
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHCC
KNCLGLIVMVQREMAEKFCAKEGNSEFSSLGVLSAMICERKILFDVDPQCFNPPPKVMSA
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
VMSLIKTKDFDELCEIENFKNFLKDCFKAPRKQLLGNLKTYKAKVLEVLSTLGLKENIRP
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
HEICVDLYLKIYDKLKDEYGRKQRDK
HHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVKAKKQYGQNFLIDKSVLAKIIQAIPKEMNNIIEIGPGLGDLTQELLKISQVKAYEIDN
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DLIPILKKKFQKELECGKFNLIHQDASEAFNPSLDEKPYFLVANLPYYVASHIILKALED
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHCC
KNCLGLIVMVQREMAEKFCAKEGNSEFSSLGVLSAMICERKILFDVDPQCFNPPPKVMSA
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
VMSLIKTKDFDELCEIENFKNFLKDCFKAPRKQLLGNLKTYKAKVLEVLSTLGLKENIRP
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
HEICVDLYLKIYDKLKDEYGRKQRDK
HHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]