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The map label for this gene is thiI

Identifier: 21228619

GI number: 21228619

Start: 3002150

End: 3003367

Strand: Reverse

Name: thiI

Synonym: MM_2517

Alternate gene names: 21228619

Gene position: 3003367-3002150 (Counterclockwise)

Preceding gene: 21228622

Following gene: 21228611

Centisome position: 73.32

GC content: 47.87

Gene sequence:

>1218_bases
ATGCAGGGTGCTTTCACAGATAATACGAATAACACGGGGTCAGAAAAAGAAACGAACGTTGTCATAGTCCGTTACGGGGA
GCTTGCTCTCAAAAGTCCTGGAGTCCGTAACTGGTATGAAAAAATACTTATGAAAAACATCGCCGCAATGCTGGACTCAA
AAAACATTCCATACTCCCTCATGCGGAGAGAATGGGGCAGGATATTTATAGAGACCACTGACCCGCGCGCAGCTGGAGCG
GCTGCGGATGTTTTTGGGGTAGTCTCAACATCCTCTGCACTGATAACCAAACCTGAGCTTGACAGCGCAGCCGAAACATG
CGCTTTTCTCGGAAAGAAAATCATCCAGGAAGGGGAATCTTTTGCAATCCGGGCCAGAAGAAGTGGAAATCATTCATTCT
CATCAGCAGATATAGGTAGAGCCTGCGGAGATGCCGTCTGGAACATGCTTGAAAGTGAAGGAAAGCATCCGAAAGTTAAC
CTCGGTTCCCCTGACAGGGAAATTTTTGTAGAAATGAGGCAGAACCTGGCTTATGTTTATCTGGAAACAGTTAAAGGAGT
GGGAGGGCTCCCTCTTGGCACCCAGGGCAAAATGGTGGTGCTTATGTCAGGAGGCCTCGATTCTCCTGTTGCTGCCTGGC
TGATGATGAAGCGGGGAGTGATGATAATCCCTGTTTACTGCAATACCTCTCCTTATGCGGAAAACGCTGCAAAGGAGCGG
GCTTATGACTGCATCCGTCAGCTCCAGACCTGGGCTTCAGGACACCAGTTCACCACCTACGAAATTCCTCATGGCCCCAA
TCTCAGGTCTTTTATAGAAATGTGCGATCGAAAAAACACCTGCCTCCTCTGCAAACGTATGATGTACAGGGAAGCCTATG
AAATCATGAAAAAAGAAGGTGCGAGTGGAATTATCACAGGCTCTTCCCTTGGGCAGGTAGCCTCCCAGACTGCTGCGAAT
ATGCATGCAGAGATCTACCAGCTTGCCATCCCTATATATCACCCCTTAATTGCCTTTGACAAGAGTGAGATTATAGACAT
TGCTCGCAAAATCGGGACCTACGATATATCTACCAGGTCTGCAGGAAGCTGCACCGCAGTCCCTGAACGCCCCGAAGTAA
AGGCAAACTATGACCTTATAGTTCTTGAAGAGAAAAGGCTTGATATAGAAAATATGGTCAGCGAAGCATTGAAAGCTGCA
AAGGTCCTCAAGCTCTGA

Upstream 100 bases:

>100_bases
CCAGTATATCTACTATTAGAGTATATGACAGACAATTCTGAAAGGCTGAAAGAGAATTTTAATCCGCTATCGATAAAGAA
TAAAGAGAGACATAAAGAAC

Downstream 100 bases:

>100_bases
TGGACTTCCTGACTTTAAGAGAATCTCAGCGTAAAGTAAATCCCGGATTCTCTTACATTTTAATTAGCAGGAAACGGAGA
AAACAGCATTTTAAAAAAGA

Product: thiamine biosynthesis protein

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MQGAFTDNTNNTGSEKETNVVIVRYGELALKSPGVRNWYEKILMKNIAAMLDSKNIPYSLMRREWGRIFIETTDPRAAGA
AADVFGVVSTSSALITKPELDSAAETCAFLGKKIIQEGESFAIRARRSGNHSFSSADIGRACGDAVWNMLESEGKHPKVN
LGSPDREIFVEMRQNLAYVYLETVKGVGGLPLGTQGKMVVLMSGGLDSPVAAWLMMKRGVMIIPVYCNTSPYAENAAKER
AYDCIRQLQTWASGHQFTTYEIPHGPNLRSFIEMCDRKNTCLLCKRMMYREAYEIMKKEGASGIITGSSLGQVASQTAAN
MHAEIYQLAIPIYHPLIAFDKSEIIDIARKIGTYDISTRSAGSCTAVPERPEVKANYDLIVLEEKRLDIENMVSEALKAA
KVLKL

Sequences:

>Translated_405_residues
MQGAFTDNTNNTGSEKETNVVIVRYGELALKSPGVRNWYEKILMKNIAAMLDSKNIPYSLMRREWGRIFIETTDPRAAGA
AADVFGVVSTSSALITKPELDSAAETCAFLGKKIIQEGESFAIRARRSGNHSFSSADIGRACGDAVWNMLESEGKHPKVN
LGSPDREIFVEMRQNLAYVYLETVKGVGGLPLGTQGKMVVLMSGGLDSPVAAWLMMKRGVMIIPVYCNTSPYAENAAKER
AYDCIRQLQTWASGHQFTTYEIPHGPNLRSFIEMCDRKNTCLLCKRMMYREAYEIMKKEGASGIITGSSLGQVASQTAAN
MHAEIYQLAIPIYHPLIAFDKSEIIDIARKIGTYDISTRSAGSCTAVPERPEVKANYDLIVLEEKRLDIENMVSEALKAA
KVLKL
>Mature_405_residues
MQGAFTDNTNNTGSEKETNVVIVRYGELALKSPGVRNWYEKILMKNIAAMLDSKNIPYSLMRREWGRIFIETTDPRAAGA
AADVFGVVSTSSALITKPELDSAAETCAFLGKKIIQEGESFAIRARRSGNHSFSSADIGRACGDAVWNMLESEGKHPKVN
LGSPDREIFVEMRQNLAYVYLETVKGVGGLPLGTQGKMVVLMSGGLDSPVAAWLMMKRGVMIIPVYCNTSPYAENAAKER
AYDCIRQLQTWASGHQFTTYEIPHGPNLRSFIEMCDRKNTCLLCKRMMYREAYEIMKKEGASGIITGSSLGQVASQTAAN
MHAEIYQLAIPIYHPLIAFDKSEIIDIARKIGTYDISTRSAGSCTAVPERPEVKANYDLIVLEEKRLDIENMVSEALKAA
KVLKL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=389, Percent_Identity=28.2776349614396, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_METMA (Q8PU39)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_634541.1
- ProteinModelPortal:   Q8PU39
- SMR:   Q8PU39
- GeneID:   1480859
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_2517
- NMPDR:   fig|192952.1.peg.2517
- HOGENOM:   HBG646072
- OMA:   RREWGRI
- ProtClustDB:   CLSK2392180
- BioCyc:   MMAZ192952:MM2517-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 44704; Mature: 44704

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS51165 THUMP

Important sites: BINDING 285-285 BINDING 307-307 BINDING 316-316

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGAFTDNTNNTGSEKETNVVIVRYGELALKSPGVRNWYEKILMKNIAAMLDSKNIPYSL
CCCCCCCCCCCCCCCCCCCEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
MRREWGRIFIETTDPRAAGAAADVFGVVSTSSALITKPELDSAAETCAFLGKKIIQEGES
HHHHHCEEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCC
FAIRARRSGNHSFSSADIGRACGDAVWNMLESEGKHPKVNLGSPDREIFVEMRQNLAYVY
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHCHHEEE
LETVKGVGGLPLGTQGKMVVLMSGGLDSPVAAWLMMKRGVMIIPVYCNTSPYAENAAKER
EEHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
AYDCIRQLQTWASGHQFTTYEIPHGPNLRSFIEMCDRKNTCLLCKRMMYREAYEIMKKEG
HHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
ASGIITGSSLGQVASQTAANMHAEIYQLAIPIYHPLIAFDKSEIIDIARKIGTYDISTRS
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEECCCCC
AGSCTAVPERPEVKANYDLIVLEEKRLDIENMVSEALKAAKVLKL
CCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQGAFTDNTNNTGSEKETNVVIVRYGELALKSPGVRNWYEKILMKNIAAMLDSKNIPYSL
CCCCCCCCCCCCCCCCCCCEEEEEECCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
MRREWGRIFIETTDPRAAGAAADVFGVVSTSSALITKPELDSAAETCAFLGKKIIQEGES
HHHHHCEEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCC
FAIRARRSGNHSFSSADIGRACGDAVWNMLESEGKHPKVNLGSPDREIFVEMRQNLAYVY
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHCHHEEE
LETVKGVGGLPLGTQGKMVVLMSGGLDSPVAAWLMMKRGVMIIPVYCNTSPYAENAAKER
EEHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
AYDCIRQLQTWASGHQFTTYEIPHGPNLRSFIEMCDRKNTCLLCKRMMYREAYEIMKKEG
HHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
ASGIITGSSLGQVASQTAANMHAEIYQLAIPIYHPLIAFDKSEIIDIARKIGTYDISTRS
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEECCCCC
AGSCTAVPERPEVKANYDLIVLEEKRLDIENMVSEALKAAKVLKL
CCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824