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The map label for this gene is mtsA [H]

Identifier: 21228529

GI number: 21228529

Start: 2896878

End: 2897981

Strand: Direct

Name: mtsA [H]

Synonym: MM_2427

Alternate gene names: 21228529

Gene position: 2896878-2897981 (Clockwise)

Preceding gene: 21228525

Following gene: 21228530

Centisome position: 70.72

GC content: 51.18

Gene sequence:

>1104_bases
ATGGTAAGCGAGATGACTCCAACCCGCCGAGCTCTGGCAGCAGTGCTCGGAGGCAGGGTCGATTATGTACCCCCTGCAAA
CCCCCTGGCTCAGACCACAACAGAATTAATGGCAATCTGCAATGCTTCCTGGCCAAAAGCCCATTTTGACAGCAAAATGA
TGGCAGATCTTGCAGCCGCTTCTTACGAAGTTTGTGGCATTGAGGCTGCGCGACCCCAGTTTGACATCTCTCTTGAAGCT
GAAGTTCTGGGGTGTAAACTTGACTGGGATAAGCCAGACAGGCCTCCGGTAACAGGACCAGCTTATACGAACCCTGAAGA
TGTGACCTGGCCCGACAAACTGGAAGAAACCGGAAGGATTCCGGTAGTTCTTGGAGCAATTGATGAACTCAGGAAACGTT
ACGACGGGATGCTCCCTATCATCCCCTTACTGACCGCCCCCTTTACGGTAGCAGGTCACATAGCAGGCGTTGAAAATATG
GCCCGGTGGACAAAAACCGATCCTGAAAAAGCCCATGCCTTCATTGATGCAGCAACAGATTTTGTTGTAGCTTACGGAAA
GCTGCAGGCTGCTTACGGGGCGCATATCCTCTTCCTTGCAGATCCTTCGGCTTCCAGCAGCCTGATTTCTGCGGAAACAT
ACCGGGAATTTGTACTCCCTGCCCACAGAAGACTGGCAAAGGAAATAAGCTGCCCCCAGATCCTGCATATCTGCGGAGAT
TCCAGTAAACTGCTGCCTTATATCAAGCAGAGCGGAATCGACTGCTTCTCCTTTGATACCGTCCCGGTCTGGTACTGCCG
TCAGGTAATTGGCAACGATATGTCCATACTCGGAAGCCTGGACGTCATAGACCTGATGCCAAACGGAACCCCAGAACAGG
TTTATAACAGGACCCGGGAATGCATACTGCAGGGGACAGATATTGTAGGGACTGCATGTGGTGTATCATACGGGACCCCG
CTTGAAAATCTCAGAGCATATGTCAGGGCCTGCAAAGAGACTCCAATCCCAAGATATGATGACGTTGAAGATCTTATCAG
GCAGATTGGAGTCGGCATCGGGCGAAACATGAAAGAAAACGTTCTGGGAGGGATGCAGGAATGA

Upstream 100 bases:

>100_bases
CCCTCAATCTGTAATCTGAATGTAAAATATATTCTCTTTAATATATTATTAAATATTCTATCTTATGTAAAAGTGTGAAT
TAAAAAAGGAGACCCAAAAT

Downstream 100 bases:

>100_bases
TCAGGCATATTGACATCGCAGTCCAGAAGATTTTCGAGATGAAAGAAAAAGAACCTGCGAAATTCAAAAAACTTATTGAT
GAAGGGATCATGATCGGCCT

Product: methylcobalamin:coenzyme M methyltransferase

Products: CO2; Coproporphyrinogen III [C]

Alternate protein names: MT2-A; Methylcobamide:CoM methyltransferase II isozyme A [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MVSEMTPTRRALAAVLGGRVDYVPPANPLAQTTTELMAICNASWPKAHFDSKMMADLAAASYEVCGIEAARPQFDISLEA
EVLGCKLDWDKPDRPPVTGPAYTNPEDVTWPDKLEETGRIPVVLGAIDELRKRYDGMLPIIPLLTAPFTVAGHIAGVENM
ARWTKTDPEKAHAFIDAATDFVVAYGKLQAAYGAHILFLADPSASSSLISAETYREFVLPAHRRLAKEISCPQILHICGD
SSKLLPYIKQSGIDCFSFDTVPVWYCRQVIGNDMSILGSLDVIDLMPNGTPEQVYNRTRECILQGTDIVGTACGVSYGTP
LENLRAYVRACKETPIPRYDDVEDLIRQIGVGIGRNMKENVLGGMQE

Sequences:

>Translated_367_residues
MVSEMTPTRRALAAVLGGRVDYVPPANPLAQTTTELMAICNASWPKAHFDSKMMADLAAASYEVCGIEAARPQFDISLEA
EVLGCKLDWDKPDRPPVTGPAYTNPEDVTWPDKLEETGRIPVVLGAIDELRKRYDGMLPIIPLLTAPFTVAGHIAGVENM
ARWTKTDPEKAHAFIDAATDFVVAYGKLQAAYGAHILFLADPSASSSLISAETYREFVLPAHRRLAKEISCPQILHICGD
SSKLLPYIKQSGIDCFSFDTVPVWYCRQVIGNDMSILGSLDVIDLMPNGTPEQVYNRTRECILQGTDIVGTACGVSYGTP
LENLRAYVRACKETPIPRYDDVEDLIRQIGVGIGRNMKENVLGGMQE
>Mature_367_residues
MVSEMTPTRRALAAVLGGRVDYVPPANPLAQTTTELMAICNASWPKAHFDSKMMADLAAASYEVCGIEAARPQFDISLEA
EVLGCKLDWDKPDRPPVTGPAYTNPEDVTWPDKLEETGRIPVVLGAIDELRKRYDGMLPIIPLLTAPFTVAGHIAGVENM
ARWTKTDPEKAHAFIDAATDFVVAYGKLQAAYGAHILFLADPSASSSLISAETYREFVLPAHRRLAKEISCPQILHICGD
SSKLLPYIKQSGIDCFSFDTVPVWYCRQVIGNDMSILGSLDVIDLMPNGTPEQVYNRTRECILQGTDIVGTACGVSYGTP
LENLRAYVRACKETPIPRYDDVEDLIRQIGVGIGRNMKENVLGGMQE

Specific function: Catalyzes the transfer of the methyl group from the methylated corrinoid cofactor of mtmC, mtbC and mttC to coenzyme M [H]

COG id: COG0407

COG function: function code H; Uroporphyrinogen-III decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uroporphyrinogen decarboxylase family. MtbA/mtaA subfamily [H]

Homologues:

Organism=Drosophila melanogaster, GI19921920, Length=258, Percent_Identity=24.4186046511628, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI221330099, Length=258, Percent_Identity=24.4186046511628, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006360
- InterPro:   IPR000257 [H]

Pfam domain/function: PF01208 URO-D [H]

EC number: 4.1.1.37 [C]

Molecular weight: Translated: 40192; Mature: 40192

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSEMTPTRRALAAVLGGRVDYVPPANPLAQTTTELMAICNASWPKAHFDSKMMADLAAA
CCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHH
SYEVCGIEAARPQFDISLEAEVLGCKLDWDKPDRPPVTGPAYTNPEDVTWPDKLEETGRI
HHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCC
PVVLGAIDELRKRYDGMLPIIPLLTAPFTVAGHIAGVENMARWTKTDPEKAHAFIDAATD
CEEEHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FVVAYGKLQAAYGAHILFLADPSASSSLISAETYREFVLPAHRRLAKEISCPQILHICGD
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
SSKLLPYIKQSGIDCFSFDTVPVWYCRQVIGNDMSILGSLDVIDLMPNGTPEQVYNRTRE
CCHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCHHHHCCCCEEEECCCCCHHHHHHHHHH
CILQGTDIVGTACGVSYGTPLENLRAYVRACKETPIPRYDDVEDLIRQIGVGIGRNMKEN
HHHCCCCCEEHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHH
VLGGMQE
HCCCCCC
>Mature Secondary Structure
MVSEMTPTRRALAAVLGGRVDYVPPANPLAQTTTELMAICNASWPKAHFDSKMMADLAAA
CCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHH
SYEVCGIEAARPQFDISLEAEVLGCKLDWDKPDRPPVTGPAYTNPEDVTWPDKLEETGRI
HHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCC
PVVLGAIDELRKRYDGMLPIIPLLTAPFTVAGHIAGVENMARWTKTDPEKAHAFIDAATD
CEEEHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FVVAYGKLQAAYGAHILFLADPSASSSLISAETYREFVLPAHRRLAKEISCPQILHICGD
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
SSKLLPYIKQSGIDCFSFDTVPVWYCRQVIGNDMSILGSLDVIDLMPNGTPEQVYNRTRE
CCHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCHHHHCCCCEEEECCCCCHHHHHHHHHH
CILQGTDIVGTACGVSYGTPLENLRAYVRACKETPIPRYDDVEDLIRQIGVGIGRNMKEN
HHHCCCCCEEHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHH
VLGGMQE
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Reducing agents [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00231 {7-COOH,} 0.00053 {8-COOH-porphyrinogen} 0.00025 {8-COOH-porphyrinogen} 0.00533 {uroporphyrinogen} 0.00256 {uroporphyrinogen} 0.00133 {uroporphyrinogen} 0.001 {uroporphyrinogen} 0.0008 {uroporphyrinogen} 0.0004 {uroporphyrino

Substrates: H+; Uroporphyrinogen III [C]

Specific reaction: (4) H+ + Uroporphyrinogen III --> (4) CO2 + Coproporphyrinogen III [C]

General reaction: Decarboxylation [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); 7-COOH-porphyrinogenI; 7-COOH-porphyrinogen III; Butanedione; Diethyl dicarbonate; Fe2+; Iodoacetamide; p-Hydroxy mercuribenzoate; Phenyl glyoxal; Pt2+; Sn2+; UroporphyrinogenI uroporphyrinogen III; Uroporphyrinogen III;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8702528; 9642198; 7635826; 8617801; 9006042; 9195968; 10852929 [H]