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The map label for this gene is dphB

Identifier: 21228457

GI number: 21228457

Start: 2813461

End: 2814261

Strand: Direct

Name: dphB

Synonym: MM_2355

Alternate gene names: 21228457

Gene position: 2813461-2814261 (Clockwise)

Preceding gene: 21228454

Following gene: 21228458

Centisome position: 68.68

GC content: 45.57

Gene sequence:

>801_bases
ATGCTCACATTTATAGGGCTGGGCCTTTTTGATGAATACGATATCTCCTTAAAAGGACTTGAAGCTGTTAAGGAAGCTGA
CCTGGTATACGCTGAGTTTTACACATCCTGTCTCATGGGAACAAACCCTGAGAAAATGGAAAAGCTCTACGGAAAGAAGG
TTCATTTACTCTCAAGGGAAGATGTGGAACAGCACCCTGACTGGCTTGATAATGCCAGAGACAAAAAAGTAGCTTTCCTC
ACCGGTGGAGATACAATGGTCTCCACAACACATGTTGACCTGCGCCTCAGAGCTGAAAAGCTCGGGATAGAGACCCGTCT
GATCCACGGGGCGTCCATCACATCAGCTGTTTCAGGGCTAACAGGGCTACAGAACTACCGGTTTGGGAAGTCTGCAAGCA
TTCCCTATCCTTATGAAAGCAGGCGAGGGACGAAAGTAATTTCTGAAACCCCTTATGATACCATAAAGCAAAATTCTTCA
TTCGGGCTTCATACTCTTGTTTTTCTGGATATCGATAAAGATAAGGGATTTATGTCCGTTAACATCGCCCTTAAGCTTCT
CCTTGAAGTTGAAAGTAAAAGGGGGGAAGGAGTGATGGATAGGGCTGTTGCTGTGGGAATAGCAAGGGCAGGCTCGGAAA
AGCCTGTAGTAAAAGCTGGTTACGCAGAGGACCTGAAAGGTTTTGATTTTGGAAAGCCTCTTCACATCCTTGTGGTTCCG
GGAAAACTGCATTTTCTGGAAGCTGAAGCCCTTGTGAAACTTGCGGATGGGCCTGAAGAAATCATGGAAAACATTGAATG
A

Upstream 100 bases:

>100_bases
ATACGTTGAGCGAATCTGTAATTCTAAAAACAAAGCTGAAGACTCCCCGGATAAGCCGTGGCGCCAAAAGCTTTAAACCA
ATAAGCTCCTAAAGCGGGTT

Downstream 100 bases:

>100_bases
AACTGAAAAAAATCTGTTGAGCAAAGAATAGCTGAATTACTGTATACACTTTCTGGTGGTCAAAATGCCTGCCGATTTGA
ATGAAAAGGTTAACCGGTAC

Product: diphthine synthase

Products: NA

Alternate protein names: Diphthamide biosynthesis methyltransferase

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MLTFIGLGLFDEYDISLKGLEAVKEADLVYAEFYTSCLMGTNPEKMEKLYGKKVHLLSREDVEQHPDWLDNARDKKVAFL
TGGDTMVSTTHVDLRLRAEKLGIETRLIHGASITSAVSGLTGLQNYRFGKSASIPYPYESRRGTKVISETPYDTIKQNSS
FGLHTLVFLDIDKDKGFMSVNIALKLLLEVESKRGEGVMDRAVAVGIARAGSEKPVVKAGYAEDLKGFDFGKPLHILVVP
GKLHFLEAEALVKLADGPEEIMENIE

Sequences:

>Translated_266_residues
MLTFIGLGLFDEYDISLKGLEAVKEADLVYAEFYTSCLMGTNPEKMEKLYGKKVHLLSREDVEQHPDWLDNARDKKVAFL
TGGDTMVSTTHVDLRLRAEKLGIETRLIHGASITSAVSGLTGLQNYRFGKSASIPYPYESRRGTKVISETPYDTIKQNSS
FGLHTLVFLDIDKDKGFMSVNIALKLLLEVESKRGEGVMDRAVAVGIARAGSEKPVVKAGYAEDLKGFDFGKPLHILVVP
GKLHFLEAEALVKLADGPEEIMENIE
>Mature_266_residues
MLTFIGLGLFDEYDISLKGLEAVKEADLVYAEFYTSCLMGTNPEKMEKLYGKKVHLLSREDVEQHPDWLDNARDKKVAFL
TGGDTMVSTTHVDLRLRAEKLGIETRLIHGASITSAVSGLTGLQNYRFGKSASIPYPYESRRGTKVISETPYDTIKQNSS
FGLHTLVFLDIDKDKGFMSVNIALKLLLEVESKRGEGVMDRAVAVGIARAGSEKPVVKAGYAEDLKGFDFGKPLHILVVP
GKLHFLEAEALVKLADGPEEIMENIE

Specific function: Required for the methylation step in diphthamide biosynthesis

COG id: COG1798

COG function: function code J; Diphthamide biosynthesis methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diphthine synthase family

Homologues:

Organism=Homo sapiens, GI117190326, Length=274, Percent_Identity=41.2408759124088, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI117190262, Length=274, Percent_Identity=41.2408759124088, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI117190324, Length=273, Percent_Identity=41.3919413919414, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17531369, Length=271, Percent_Identity=35.7933579335793, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323201, Length=284, Percent_Identity=39.7887323943662, Blast_Score=164, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28572120, Length=278, Percent_Identity=37.7697841726619, Blast_Score=153, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPHB_METMA (Q8PUH9)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_634379.1
- ProteinModelPortal:   Q8PUH9
- SMR:   Q8PUH9
- GeneID:   1480697
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_2355
- NMPDR:   fig|192952.1.peg.2355
- HOGENOM:   HBG460511
- OMA:   YAEFYTA
- ProtClustDB:   PRK04160
- BioCyc:   MMAZ192952:MM2355-MONOMER
- BRENDA:   2.1.1.98
- HAMAP:   MF_01084
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR004551
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- PANTHER:   PTHR10882
- PIRSF:   PIRSF036432
- TIGRFAMs:   TIGR00522

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.98

Molecular weight: Translated: 29397; Mature: 29397

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTFIGLGLFDEYDISLKGLEAVKEADLVYAEFYTSCLMGTNPEKMEKLYGKKVHLLSRE
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEECHH
DVEQHPDWLDNARDKKVAFLTGGDTMVSTTHVDLRLRAEKLGIETRLIHGASITSAVSGL
HHHHCCCHHCCCCCCEEEEEECCCEEEEEEEEEEEEEHHHHCCEEEEEECCHHHHHHHHH
TGLQNYRFGKSASIPYPYESRRGTKVISETPYDTIKQNSSFGLHTLVFLDIDKDKGFMSV
HHHHHCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHCCCCCCEEEEEEEEECCCCCEEEH
NIALKLLLEVESKRGEGVMDRAVAVGIARAGSEKPVVKAGYAEDLKGFDFGKPLHILVVP
HHHHHEEHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHCCCCCCCCCEEEEEEC
GKLHFLEAEALVKLADGPEEIMENIE
CEEEEEEHHHHEEECCCHHHHHHCCC
>Mature Secondary Structure
MLTFIGLGLFDEYDISLKGLEAVKEADLVYAEFYTSCLMGTNPEKMEKLYGKKVHLLSRE
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEECHH
DVEQHPDWLDNARDKKVAFLTGGDTMVSTTHVDLRLRAEKLGIETRLIHGASITSAVSGL
HHHHCCCHHCCCCCCEEEEEECCCEEEEEEEEEEEEEHHHHCCEEEEEECCHHHHHHHHH
TGLQNYRFGKSASIPYPYESRRGTKVISETPYDTIKQNSSFGLHTLVFLDIDKDKGFMSV
HHHHHCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHCCCCCCEEEEEEEEECCCCCEEEH
NIALKLLLEVESKRGEGVMDRAVAVGIARAGSEKPVVKAGYAEDLKGFDFGKPLHILVVP
HHHHHEEHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHCCCCCCCCCEEEEEEC
GKLHFLEAEALVKLADGPEEIMENIE
CEEEEEEHHHHEEECCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824