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The map label for this gene is echC [H]

Identifier: 21228424

GI number: 21228424

Start: 2781213

End: 2781683

Strand: Direct

Name: echC [H]

Synonym: MM_2322

Alternate gene names: 21228424

Gene position: 2781213-2781683 (Clockwise)

Preceding gene: 21228423

Following gene: 21228425

Centisome position: 67.89

GC content: 41.61

Gene sequence:

>471_bases
ATGAGTTTAGCAAAATCACCATGGATCATACATGTGAATTGTAACAGCTGCAATGGCTGTGACATTGAGGTAGTAGCCTG
TCTTACCCCTTTATATGATGCTGAAAGATTTGGCGTTTTAAATATCGGTACTCCCAAGCAAGCTGATATAATGGTAGTTA
CAGGCTCTGTAAATTATAAAAATGTCAATGTACTCAAAAATCTTTACAACCAGATCCCGGACCCGAAGGTTGTTTTAGCT
GTCGGTGCCTGTGCATCTACTGGTGGGATATTCCATAACTGCTATAATGTTATAGGCGGAGTGGACCAGATAATACCCGT
AGATGCATATGTTCCCGGGTGCTGTCCAAGGCCCGAAGCCATACTTGACGGCGTAGTAGCAGCACTTTCAATACTTGAAA
ATAAGAAGAAAGGGAACATCAAAGGAAAAGAAGTACCATTGAAAGGAAAAGAGGTGCCATCGAATGCTTAA

Upstream 100 bases:

>100_bases
ATGCTCAAATTGAGCTGGACATTCCTGTTAGCAATTTCACTTGTGAACATAGTGTACCTGTACGTAAGTGGAGTAAAATT
AGTGTGAAAGAGGGCTATTT

Downstream 100 bases:

>100_bases
GAATGAGGTAGAAATTAAAAGTAGCGAAGAGCTGCTAAAAACCGTAGAGCAATTAAAAAAGGATGGCTATCGAAACGCTA
CTATGATATGCCTGAAAGCT

Product: ech hydrogenase subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MSLAKSPWIIHVNCNSCNGCDIEVVACLTPLYDAERFGVLNIGTPKQADIMVVTGSVNYKNVNVLKNLYNQIPDPKVVLA
VGACASTGGIFHNCYNVIGGVDQIIPVDAYVPGCCPRPEAILDGVVAALSILENKKKGNIKGKEVPLKGKEVPSNA

Sequences:

>Translated_156_residues
MSLAKSPWIIHVNCNSCNGCDIEVVACLTPLYDAERFGVLNIGTPKQADIMVVTGSVNYKNVNVLKNLYNQIPDPKVVLA
VGACASTGGIFHNCYNVIGGVDQIIPVDAYVPGCCPRPEAILDGVVAALSILENKKKGNIKGKEVPLKGKEVPSNA
>Mature_155_residues
SLAKSPWIIHVNCNSCNGCDIEVVACLTPLYDAERFGVLNIGTPKQADIMVVTGSVNYKNVNVLKNLYNQIPDPKVVLAV
GACASTGGIFHNCYNVIGGVDQIIPVDAYVPGCCPRPEAILDGVVAALSILENKKKGNIKGKEVPLKGKEVPSNA

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=121, Percent_Identity=38.0165289256198, Blast_Score=116, Evalue=8e-27,
Organism=Escherichia coli, GI1789074, Length=133, Percent_Identity=45.8646616541353, Blast_Score=141, Evalue=2e-35,
Organism=Escherichia coli, GI1788834, Length=133, Percent_Identity=41.3533834586466, Blast_Score=130, Evalue=3e-32,
Organism=Escherichia coli, GI1788624, Length=108, Percent_Identity=44.4444444444444, Blast_Score=105, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17509685, Length=121, Percent_Identity=39.6694214876033, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18859983, Length=107, Percent_Identity=42.9906542056075, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24642371, Length=107, Percent_Identity=42.9906542056075, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24651058, Length=107, Percent_Identity=40.1869158878505, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 16521; Mature: 16390

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAKSPWIIHVNCNSCNGCDIEVVACLTPLYDAERFGVLNIGTPKQADIMVVTGSVNYK
CCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCEEEEEECCCCCC
NVNVLKNLYNQIPDPKVVLAVGACASTGGIFHNCYNVIGGVDQIIPVDAYVPGCCPRPEA
HHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHH
ILDGVVAALSILENKKKGNIKGKEVPLKGKEVPSNA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLAKSPWIIHVNCNSCNGCDIEVVACLTPLYDAERFGVLNIGTPKQADIMVVTGSVNYK
CCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCEEEEEECCCCCC
NVNVLKNLYNQIPDPKVVLAVGACASTGGIFHNCYNVIGGVDQIIPVDAYVPGCCPRPEA
HHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHH
ILDGVVAALSILENKKKGNIKGKEVPLKGKEVPSNA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]