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The map label for this gene is rpoA2

Identifier: 21228372

GI number: 21228372

Start: 2695038

End: 2696264

Strand: Reverse

Name: rpoA2

Synonym: MM_2270

Alternate gene names: 21228372

Gene position: 2696264-2695038 (Counterclockwise)

Preceding gene: 21228373

Following gene: 21228371

Centisome position: 65.82

GC content: 47.92

Gene sequence:

>1227_bases
ATGATGAGCATCAGCGAAGCCACAGTAGACAGCATGATAAAGGACCTTCCACTTCCTTCTAACATTTTAAAAACTCTGAG
GGACGATGTTATCAAGGCAGGGGTAAGCAAAAAAGAAATGGAAGAGATTATAGAAAGGGTAATGGAAGAATATACGGTCT
CGTGCATTGAGCCCTGTGACGCTGCTGGAGTTGTTGCAGCCCAGTCAATAGGAGAACCGGGCACTCAGATGACGATGCGT
ACCTTCCACTACGCCGGTGTGGCTGAGATTAACGTTACCCTGGGTCTTCCTCGTTTGATTGAAATCGTGGATGCAAGGAA
GATTCCGAGCACTCCTATGATGACAATTGCCCTTTCCAAAGAACACCCGGAAGACTATGCTTACGACAGGGAAAAGACAA
GAGCCCTTGCATGGGAGATTGAAGCAACCAAAATAGACCATATCGCTGACGTTACCACTGACCTTTCCCAGATGAAGCTT
ATAATAGACCTGCACGAAAAAGCAATGGAAGGCAGGAATATTACAATAGACCGGGTAAAGGAGAAGTTCAATGAAGAGCT
CAATGTCCTGGTCAGCATCTCTCCTGATATAGACAACCAGATTGTTATCACTCCCGGAGAGCCTTCTTACAGGGAACTGC
TCCAGCTTGCAAAGAGCATCCATAATGTTACCCTCAAAGGCATAGAAGGGATCAAAAGAGTCGTGGTCCGAAAAGAGGGC
GAAGAATATACCCTGTATACAGAAGGGTCGGCTCTTCGTGAAGTCCTGCAGTTTGAAGGCGTGGACAGGACCAGAACCAG
CACAAACAACATTAACGAAATTTACGAAGTGTTGGGGATCGAGGCTGCAAGAAACGCAATCATCAAGGAGGCTACGGATA
CCCTGCGTGAACAGGGTCTTACCGTGGACATCAGGCACATCATGCTTGTGGCAGACCTTATGACTTCGGACGGGGAAGTA
AAACAGATAGGCAGGCACGGTATTTCAGGTGAAAAGGCAAGTGTATTTGCCCGTGCGGCTTTCGAAGTTACTGTGAACCA
TCTTCTCGATGCAGGTATGCGCGGATACGTGGACCAGCTGCAGGGTGTTACGGAAAACATCATTGTCGGCCAGCCGATCC
GCATGGGTACGGGAGACGTACACCTTATAAGCAGAAAAGCAGAGAAAGTGGTGGAAGTACCACCGGAAATCGAAACAGCA
GAAGAAATTGAAGTCGAAGAGGGGTGA

Upstream 100 bases:

>100_bases
TCAAGTACGGGGAAGATGGAATTAACCCGACAAAGAGTGATTTCAGCAAACCCGACACTATCCACAGGATTGTCCGTTCC
GTAGTAAACAAGGAGGTGGC

Downstream 100 bases:

>100_bases
AGTCCGGTAAAACCCGGACACGACCTATAATTATTTAAATGTATAATCTGATACAGGACGGGTCCATATCGGACACATAT
TACCTTAATAAACTTCATCA

Product: DNA-directed RNA polymerase subunit A''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MMSISEATVDSMIKDLPLPSNILKTLRDDVIKAGVSKKEMEEIIERVMEEYTVSCIEPCDAAGVVAAQSIGEPGTQMTMR
TFHYAGVAEINVTLGLPRLIEIVDARKIPSTPMMTIALSKEHPEDYAYDREKTRALAWEIEATKIDHIADVTTDLSQMKL
IIDLHEKAMEGRNITIDRVKEKFNEELNVLVSISPDIDNQIVITPGEPSYRELLQLAKSIHNVTLKGIEGIKRVVVRKEG
EEYTLYTEGSALREVLQFEGVDRTRTSTNNINEIYEVLGIEAARNAIIKEATDTLREQGLTVDIRHIMLVADLMTSDGEV
KQIGRHGISGEKASVFARAAFEVTVNHLLDAGMRGYVDQLQGVTENIIVGQPIRMGTGDVHLISRKAEKVVEVPPEIETA
EEIEVEEG

Sequences:

>Translated_408_residues
MMSISEATVDSMIKDLPLPSNILKTLRDDVIKAGVSKKEMEEIIERVMEEYTVSCIEPCDAAGVVAAQSIGEPGTQMTMR
TFHYAGVAEINVTLGLPRLIEIVDARKIPSTPMMTIALSKEHPEDYAYDREKTRALAWEIEATKIDHIADVTTDLSQMKL
IIDLHEKAMEGRNITIDRVKEKFNEELNVLVSISPDIDNQIVITPGEPSYRELLQLAKSIHNVTLKGIEGIKRVVVRKEG
EEYTLYTEGSALREVLQFEGVDRTRTSTNNINEIYEVLGIEAARNAIIKEATDTLREQGLTVDIRHIMLVADLMTSDGEV
KQIGRHGISGEKASVFARAAFEVTVNHLLDAGMRGYVDQLQGVTENIIVGQPIRMGTGDVHLISRKAEKVVEVPPEIETA
EEIEVEEG
>Mature_408_residues
MMSISEATVDSMIKDLPLPSNILKTLRDDVIKAGVSKKEMEEIIERVMEEYTVSCIEPCDAAGVVAAQSIGEPGTQMTMR
TFHYAGVAEINVTLGLPRLIEIVDARKIPSTPMMTIALSKEHPEDYAYDREKTRALAWEIEATKIDHIADVTTDLSQMKL
IIDLHEKAMEGRNITIDRVKEKFNEELNVLVSISPDIDNQIVITPGEPSYRELLQLAKSIHNVTLKGIEGIKRVVVRKEG
EEYTLYTEGSALREVLQFEGVDRTRTSTNNINEIYEVLGIEAARNAIIKEATDTLREQGLTVDIRHIMLVADLMTSDGEV
KQIGRHGISGEKASVFARAAFEVTVNHLLDAGMRGYVDQLQGVTENIIVGQPIRMGTGDVHLISRKAEKVVEVPPEIETA
EEIEVEEG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family

Homologues:

Organism=Homo sapiens, GI39725938, Length=366, Percent_Identity=36.0655737704918, Blast_Score=215, Evalue=7e-56,
Organism=Homo sapiens, GI4505939, Length=437, Percent_Identity=29.7482837528604, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI103471997, Length=164, Percent_Identity=34.7560975609756, Blast_Score=87, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25145495, Length=378, Percent_Identity=35.7142857142857, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI71987878, Length=440, Percent_Identity=30, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71998295, Length=80, Percent_Identity=48.75, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324690, Length=377, Percent_Identity=37.1352785145889, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6320061, Length=439, Percent_Identity=31.8906605922551, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6324917, Length=117, Percent_Identity=40.1709401709402, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI281360912, Length=382, Percent_Identity=37.696335078534, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI17530899, Length=434, Percent_Identity=32.0276497695853, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI17647875, Length=160, Percent_Identity=33.125, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPOA2_METMA (Q8PUR2)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_634294.1
- ProteinModelPortal:   Q8PUR2
- SMR:   Q8PUR2
- GeneID:   1480612
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_2270
- NMPDR:   fig|192952.1.peg.2270
- HOGENOM:   HBG297327
- OMA:   LVADMMT
- ProtClustDB:   PRK04309
- BioCyc:   MMAZ192952:MM2270-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00411
- InterPro:   IPR007081
- InterPro:   IPR012757
- PANTHER:   PTHR19376:SF13
- TIGRFAMs:   TIGR02389

Pfam domain/function: PF04998 RNA_pol_Rpb1_5

EC number: =2.7.7.6

Molecular weight: Translated: 45333; Mature: 45333

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSISEATVDSMIKDLPLPSNILKTLRDDVIKAGVSKKEMEEIIERVMEEYTVSCIEPCD
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
AAGVVAAQSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIVDARKIPSTPMMTIALSK
CCCHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEECCHHHHHHHHHCCCCCCCEEEEEECC
EHPEDYAYDREKTRALAWEIEATKIDHIADVTTDLSQMKLIIDLHEKAMEGRNITIDRVK
CCCCHHHCCHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
EKFNEELNVLVSISPDIDNQIVITPGEPSYRELLQLAKSIHNVTLKGIEGIKRVVVRKEG
HHHCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EEYTLYTEGSALREVLQFEGVDRTRTSTNNINEIYEVLGIEAARNAIIKEATDTLREQGL
CEEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TVDIRHIMLVADLMTSDGEVKQIGRHGISGEKASVFARAAFEVTVNHLLDAGMRGYVDQL
EEEHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGVTENIIVGQPIRMGTGDVHLISRKAEKVVEVPPEIETAEEIEVEEG
CCCHHCCEECCCCCCCCCCEEEHHHHHHHHHCCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MMSISEATVDSMIKDLPLPSNILKTLRDDVIKAGVSKKEMEEIIERVMEEYTVSCIEPCD
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
AAGVVAAQSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIVDARKIPSTPMMTIALSK
CCCHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEECCHHHHHHHHHCCCCCCCEEEEEECC
EHPEDYAYDREKTRALAWEIEATKIDHIADVTTDLSQMKLIIDLHEKAMEGRNITIDRVK
CCCCHHHCCHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
EKFNEELNVLVSISPDIDNQIVITPGEPSYRELLQLAKSIHNVTLKGIEGIKRVVVRKEG
HHHCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EEYTLYTEGSALREVLQFEGVDRTRTSTNNINEIYEVLGIEAARNAIIKEATDTLREQGL
CEEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TVDIRHIMLVADLMTSDGEVKQIGRHGISGEKASVFARAAFEVTVNHLLDAGMRGYVDQL
EEEHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGVTENIIVGQPIRMGTGDVHLISRKAEKVVEVPPEIETAEEIEVEEG
CCCHHCCEECCCCCCCCCCEEEHHHHHHHHHCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824