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The map label for this gene is spsI [H]

Identifier: 21228318

GI number: 21228318

Start: 2640482

End: 2641228

Strand: Reverse

Name: spsI [H]

Synonym: MM_2216

Alternate gene names: 21228318

Gene position: 2641228-2640482 (Counterclockwise)

Preceding gene: 21228320

Following gene: 21228317

Centisome position: 64.48

GC content: 41.23

Gene sequence:

>747_bases
TTGAGAAAAAGTATAAAAAAAGGAGTAATATCTATGAAAGGTGTAATCCTCGCAGGAGGCACAGGCTCTCGCCTCTATCC
GCTTACTAAAGTAACAAACAAGCACCTGCTACCAGTATACGATAAGCCGATGATTTACTATCCCATACAGACTCTTATTA
ATGCAGGAATCAAAGAGATTATGATCGTTTCAGGAAAAGGTCATGCCGGACACTTTCTGGAATTGCTAGGCTCAGGTTCC
GAATTAGGTGTCCGTCTAACCTATGAAATCCAGGAGGAGGCCGGAGGAATTGCTCAGGCACTTGGACTTGCGGAAGACTT
TGCTGACAATAGTCCAGTGACAATGATTCTTGGAGATAATATTTTTCAGGATAATATCATTGATTCCGTAAAGTCTTTCA
CATCAGGAGCAAAGATATTTCTCAAAGAGGTTTCCGATGCTCACAGATTTGGAGTCGCTGAAATTGATGGAAACCGGATA
ATTTCTATAGAAGAAAAGCCTAAAATCCCGAAATCCAGCCTTGCAGTTACGGGCCTGTACATCTATGACTCCAGAGTCTT
TGAGATAATACGCAACCTGAAACCCTCCGGAAGAGGGGAGCTTGAGATTACCGATGTCAATAACGCATATGTTAGGATGG
GTGAAATGGAATACTCAATACTTGAAGGATTCTGGAGTGACGCGGGTACTTTTGAATCTTTATTCCGGGCAAGTGAGCTT
GTAAAAAACTTAAATAATAAAAACTAA

Upstream 100 bases:

>100_bases
AGGAACAGTAAAATGCCCTGGGGTTCTGAGTATTCTATCCTGACAAATGCAAAATTATCCCTCCAGACCATGAAAAGGAA
CCCATATTAAATATCCTGAA

Downstream 100 bases:

>100_bases
CGGAAAAACGAATATGTATATTTTTGAATACAGCGAGTTAATCCGAAATCTGGTAATAAGCGACCTCAAGGTCAAATACC
AGAGTTCTTTCTTAGGTTTT

Product: glucose-1-phosphate thymidylyltransferase

Products: diphosphate; dTDPglucose

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRKSIKKGVISMKGVILAGGTGSRLYPLTKVTNKHLLPVYDKPMIYYPIQTLINAGIKEIMIVSGKGHAGHFLELLGSGS
ELGVRLTYEIQEEAGGIAQALGLAEDFADNSPVTMILGDNIFQDNIIDSVKSFTSGAKIFLKEVSDAHRFGVAEIDGNRI
ISIEEKPKIPKSSLAVTGLYIYDSRVFEIIRNLKPSGRGELEITDVNNAYVRMGEMEYSILEGFWSDAGTFESLFRASEL
VKNLNNKN

Sequences:

>Translated_248_residues
MRKSIKKGVISMKGVILAGGTGSRLYPLTKVTNKHLLPVYDKPMIYYPIQTLINAGIKEIMIVSGKGHAGHFLELLGSGS
ELGVRLTYEIQEEAGGIAQALGLAEDFADNSPVTMILGDNIFQDNIIDSVKSFTSGAKIFLKEVSDAHRFGVAEIDGNRI
ISIEEKPKIPKSSLAVTGLYIYDSRVFEIIRNLKPSGRGELEITDVNNAYVRMGEMEYSILEGFWSDAGTFESLFRASEL
VKNLNNKN
>Mature_248_residues
MRKSIKKGVISMKGVILAGGTGSRLYPLTKVTNKHLLPVYDKPMIYYPIQTLINAGIKEIMIVSGKGHAGHFLELLGSGS
ELGVRLTYEIQEEAGGIAQALGLAEDFADNSPVTMILGDNIFQDNIIDSVKSFTSGAKIFLKEVSDAHRFGVAEIDGNRI
ISIEEKPKIPKSSLAVTGLYIYDSRVFEIIRNLKPSGRGELEITDVNNAYVRMGEMEYSILEGFWSDAGTFESLFRASEL
VKNLNNKN

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=225, Percent_Identity=28.4444444444444, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI11761619, Length=225, Percent_Identity=28.4444444444444, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1790224, Length=244, Percent_Identity=48.7704918032787, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI1788351, Length=243, Percent_Identity=45.679012345679, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1787488, Length=258, Percent_Identity=25.968992248062, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788355, Length=262, Percent_Identity=22.9007633587786, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133931050, Length=244, Percent_Identity=27.0491803278689, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320148, Length=240, Percent_Identity=31.6666666666667, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21355443, Length=223, Percent_Identity=27.3542600896861, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24644084, Length=223, Percent_Identity=27.3542600896861, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: 2.7.7.24

Molecular weight: Translated: 27250; Mature: 27250

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSIKKGVISMKGVILAGGTGSRLYPLTKVTNKHLLPVYDKPMIYYPIQTLINAGIKEI
CCCCHHHCHHEECCEEEECCCCCCEEEEHHHCCCCCCEECCCCEEEECHHHHHHCCCCEE
MIVSGKGHAGHFLELLGSGSELGVRLTYEIQEEAGGIAQALGLAEDFADNSPVTMILGDN
EEECCCCCCHHHHHHHCCCCCCEEEEEEEECHHCCHHHHHHHHHHHHCCCCCEEEEECCC
IFQDNIIDSVKSFTSGAKIFLKEVSDAHRFGVAEIDGNRIISIEEKPKIPKSSLAVTGLY
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCCCCCCEEEEEEE
IYDSRVFEIIRNLKPSGRGELEITDVNNAYVRMGEMEYSILEGFWSDAGTFESLFRASEL
EEHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHH
VKNLNNKN
HHHCCCCC
>Mature Secondary Structure
MRKSIKKGVISMKGVILAGGTGSRLYPLTKVTNKHLLPVYDKPMIYYPIQTLINAGIKEI
CCCCHHHCHHEECCEEEECCCCCCEEEEHHHCCCCCCEECCCCEEEECHHHHHHCCCCEE
MIVSGKGHAGHFLELLGSGSELGVRLTYEIQEEAGGIAQALGLAEDFADNSPVTMILGDN
EEECCCCCCHHHHHHHCCCCCCEEEEEEEECHHCCHHHHHHHHHHHHCCCCCEEEEECCC
IFQDNIIDSVKSFTSGAKIFLKEVSDAHRFGVAEIDGNRIISIEEKPKIPKSSLAVTGLY
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCCCCCCEEEEEEE
IYDSRVFEIIRNLKPSGRGELEITDVNNAYVRMGEMEYSILEGFWSDAGTFESLFRASEL
EEHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHH
VKNLNNKN
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; alpha-D-glucose 1-phosphate

Specific reaction: dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]