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The map label for this gene is hybC [C]

Identifier: 21228278

GI number: 21228278

Start: 2579100

End: 2580890

Strand: Direct

Name: hybC [C]

Synonym: MM_2176

Alternate gene names: 21228278

Gene position: 2579100-2580890 (Clockwise)

Preceding gene: 21228277

Following gene: 21228279

Centisome position: 62.96

GC content: 47.68

Gene sequence:

>1791_bases
ATGGTGCAGGTCACAGTAGACCCCCTTTCAAGAATTGAAGGGCATTACAGGATTAATACTGAAGTCGATGGAAATGGTGT
GATCACTGATGCCCAGAGCAACGGCACTGTCTTCAGGGGATTTGAAAGGTTCCTCCAGAATCATGACCCAAGAGATGCAG
CACTAATGACAATGAGGATATGTGGCGTCTGTCCTGTATGCCACAGTATAGCTGCTTCTAATGCCCTGGATGAACTTTTT
GGAGTTGCGGACCAGGTGCCTAAGGACGCACTTGTTATGAGGAATATTCACCAGGGGATGAATTTTTTAGCCAGCCATGC
AGCGCATGTTTATGTGCTCTTTGGCCCGGATCTTGCAAATCCGGCTTACCATGATATTCTTACACAGTATGGAGATACTG
GAAATGCAGTATGGGACGAGTTATCCGGGCGTTTTATGCCTCTGATAAATCACTCTAACGGTACCCACTACCCTGCTGGC
TCTTCTTATCTTGGTGCTTTAAGGGAGTTCCGCCGCCTTCATGAGGGCATTTCTCTTGTTGCAGGAAAAATGCCCCATTC
TGTTTTACAGAACGTCGGAGGCGTGGTTTATTCCCCGACAGTTGCAGATATTCAGCAGCTTGGGGTATATCTCTCACAGA
CCTTAAAGTTCGTAGAATCATTCACTCTTGGGGTTCCTCTGGAGACATGGTTAGAAAACACTTACAGGGCTTCTTCTCCT
GAGAGAGCTGTCAATTTTGTCGTCGAACACCTTCAGGAGCTTGTGGACAATTCCCTTACAAACAATGATTTTTCGCACTC
TACCGGGTGGGGAGATGCCCCTCTTTTTGCAGCCTTTGGTTCGGAGCTTGTTGGAGAAACACTTCTCGGGCTGCCAGTTA
GTTTGAAATTAGACCGGTCAGGGGGTTATGAGGACCCCAATACAATTGGTTTTCTTTCCTATGGCGTTTTTTTCAAGCCA
GAGCAGGGAGATGGTTATGACCCGACAAGCCCTGCAGAGGACAGAGTAATTCCTTCAGGTTATATGGACGGGAATTTGCA
GCCTGAAAAATTCGACCACACGAAAATTTCTGAGAATATTACTCATGCCTTTTACATTGATGAGGAGGCGGATCGCCCTC
CCTGGAATGGGCTTACAGTTCCTGAGGACGATCCTGATGAAATTGATTACACACGGGGTTCTGAAAGCCGCTATTCCTGG
ATAAAGGCGCCCCATTATGATGGCACCCCCTGTGAGGTCGGCCCAATTTCCCGTCTTCTGATCATGGAAGAGCCTCTTAT
TACGGGACTGGCAAATGCTTTTGAAGAAAATGGGTACTCCCCTGTGAACAACTATACACGTATGCTTGCAAGGATGCATG
AAATCCTTTTATTAATTCCTGAAATCATGACGTGGATTGTAGAGGACCTTACGCCAAATGCAAAGGTAGCAGTACACACG
GAACTTTCCATGGCAAGAAACTCAGAAGGCATGGGCCTGTGGGAAGCCCCGCGTGGCGCCCTCGGGCACTGGGTGGCTTC
CGGCTCTAATTCAATGACAACTCTGTACCAGGCTGTAGTTCCAAGCACCTGGAACCTCGCCCCGAGAAATGCTCAGGGAA
TTCCAAGCCCTGTTGAGCAGGCTCTTATAGGCACTAAAATCTCGGCTGCTGAAAATGCGCTTGGGGTAGACTATTCAAAT
CCTCTTGGAATCCTGCATACTGCCCGCTCTTATGATCCCTGCCTTGCATGTGCTATTCATACAATTGATAAAACTGAAAA
GAATCCGGATCGCATAATCAGGGTGGTTTAA

Upstream 100 bases:

>100_bases
CAGGTGCTCATGCCTTAAGAAGGACGGTTTTCAAAAAGCCAGAGGAGAAAGAAGGCACTTCCGGGGAAAATGTTCCTGAG
GAGACAGGGGGCAGAGAATA

Downstream 100 bases:

>100_bases
AGGGGGATGAAAATATGGTACGGTCTGCTGAAAAGCCCGCTTCCAGAAGACTGGTTGTTGAACGCTATACCTGGCTTGAA
AGGGTTACCCATCTGGTCCA

Product: F420-nonreducing hydrogenase III, large subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MVQVTVDPLSRIEGHYRINTEVDGNGVITDAQSNGTVFRGFERFLQNHDPRDAALMTMRICGVCPVCHSIAASNALDELF
GVADQVPKDALVMRNIHQGMNFLASHAAHVYVLFGPDLANPAYHDILTQYGDTGNAVWDELSGRFMPLINHSNGTHYPAG
SSYLGALREFRRLHEGISLVAGKMPHSVLQNVGGVVYSPTVADIQQLGVYLSQTLKFVESFTLGVPLETWLENTYRASSP
ERAVNFVVEHLQELVDNSLTNNDFSHSTGWGDAPLFAAFGSELVGETLLGLPVSLKLDRSGGYEDPNTIGFLSYGVFFKP
EQGDGYDPTSPAEDRVIPSGYMDGNLQPEKFDHTKISENITHAFYIDEEADRPPWNGLTVPEDDPDEIDYTRGSESRYSW
IKAPHYDGTPCEVGPISRLLIMEEPLITGLANAFEENGYSPVNNYTRMLARMHEILLLIPEIMTWIVEDLTPNAKVAVHT
ELSMARNSEGMGLWEAPRGALGHWVASGSNSMTTLYQAVVPSTWNLAPRNAQGIPSPVEQALIGTKISAAENALGVDYSN
PLGILHTARSYDPCLACAIHTIDKTEKNPDRIIRVV

Sequences:

>Translated_596_residues
MVQVTVDPLSRIEGHYRINTEVDGNGVITDAQSNGTVFRGFERFLQNHDPRDAALMTMRICGVCPVCHSIAASNALDELF
GVADQVPKDALVMRNIHQGMNFLASHAAHVYVLFGPDLANPAYHDILTQYGDTGNAVWDELSGRFMPLINHSNGTHYPAG
SSYLGALREFRRLHEGISLVAGKMPHSVLQNVGGVVYSPTVADIQQLGVYLSQTLKFVESFTLGVPLETWLENTYRASSP
ERAVNFVVEHLQELVDNSLTNNDFSHSTGWGDAPLFAAFGSELVGETLLGLPVSLKLDRSGGYEDPNTIGFLSYGVFFKP
EQGDGYDPTSPAEDRVIPSGYMDGNLQPEKFDHTKISENITHAFYIDEEADRPPWNGLTVPEDDPDEIDYTRGSESRYSW
IKAPHYDGTPCEVGPISRLLIMEEPLITGLANAFEENGYSPVNNYTRMLARMHEILLLIPEIMTWIVEDLTPNAKVAVHT
ELSMARNSEGMGLWEAPRGALGHWVASGSNSMTTLYQAVVPSTWNLAPRNAQGIPSPVEQALIGTKISAAENALGVDYSN
PLGILHTARSYDPCLACAIHTIDKTEKNPDRIIRVV
>Mature_596_residues
MVQVTVDPLSRIEGHYRINTEVDGNGVITDAQSNGTVFRGFERFLQNHDPRDAALMTMRICGVCPVCHSIAASNALDELF
GVADQVPKDALVMRNIHQGMNFLASHAAHVYVLFGPDLANPAYHDILTQYGDTGNAVWDELSGRFMPLINHSNGTHYPAG
SSYLGALREFRRLHEGISLVAGKMPHSVLQNVGGVVYSPTVADIQQLGVYLSQTLKFVESFTLGVPLETWLENTYRASSP
ERAVNFVVEHLQELVDNSLTNNDFSHSTGWGDAPLFAAFGSELVGETLLGLPVSLKLDRSGGYEDPNTIGFLSYGVFFKP
EQGDGYDPTSPAEDRVIPSGYMDGNLQPEKFDHTKISENITHAFYIDEEADRPPWNGLTVPEDDPDEIDYTRGSESRYSW
IKAPHYDGTPCEVGPISRLLIMEEPLITGLANAFEENGYSPVNNYTRMLARMHEILLLIPEIMTWIVEDLTPNAKVAVHT
ELSMARNSEGMGLWEAPRGALGHWVASGSNSMTTLYQAVVPSTWNLAPRNAQGIPSPVEQALIGTKISAAENALGVDYSN
PLGILHTARSYDPCLACAIHTIDKTEKNPDRIIRVV

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=639, Percent_Identity=27.0735524256651, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1787207, Length=300, Percent_Identity=31.6666666666667, Blast_Score=121, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 65497; Mature: 65497

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQVTVDPLSRIEGHYRINTEVDGNGVITDAQSNGTVFRGFERFLQNHDPRDAALMTMRI
CEEEEECHHHHCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
CGVCPVCHSIAASNALDELFGVADQVPKDALVMRNIHQGMNFLASHAAHVYVLFGPDLAN
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
PAYHDILTQYGDTGNAVWDELSGRFMPLINHSNGTHYPAGSSYLGALREFRRLHEGISLV
HHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AGKMPHSVLQNVGGVVYSPTVADIQQLGVYLSQTLKFVESFTLGVPLETWLENTYRASSP
HCCCCHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCH
ERAVNFVVEHLQELVDNSLTNNDFSHSTGWGDAPLFAAFGSELVGETLLGLPVSLKLDRS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCC
GGYEDPNTIGFLSYGVFFKPEQGDGYDPTSPAEDRVIPSGYMDGNLQPEKFDHTKISENI
CCCCCCCCEEEEEECEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
THAFYIDEEADRPPWNGLTVPEDDPDEIDYTRGSESRYSWIKAPHYDGTPCEVGPISRLL
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHH
IMEEPLITGLANAFEENGYSPVNNYTRMLARMHEILLLIPEIMTWIVEDLTPNAKVAVHT
HHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ELSMARNSEGMGLWEAPRGALGHWVASGSNSMTTLYQAVVPSTWNLAPRNAQGIPSPVEQ
HHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
ALIGTKISAAENALGVDYSNPLGILHTARSYDPCLACAIHTIDKTEKNPDRIIRVV
HHHCCHHHHHCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCHHHEEEEC
>Mature Secondary Structure
MVQVTVDPLSRIEGHYRINTEVDGNGVITDAQSNGTVFRGFERFLQNHDPRDAALMTMRI
CEEEEECHHHHCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
CGVCPVCHSIAASNALDELFGVADQVPKDALVMRNIHQGMNFLASHAAHVYVLFGPDLAN
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
PAYHDILTQYGDTGNAVWDELSGRFMPLINHSNGTHYPAGSSYLGALREFRRLHEGISLV
HHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AGKMPHSVLQNVGGVVYSPTVADIQQLGVYLSQTLKFVESFTLGVPLETWLENTYRASSP
HCCCCHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCH
ERAVNFVVEHLQELVDNSLTNNDFSHSTGWGDAPLFAAFGSELVGETLLGLPVSLKLDRS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCC
GGYEDPNTIGFLSYGVFFKPEQGDGYDPTSPAEDRVIPSGYMDGNLQPEKFDHTKISENI
CCCCCCCCEEEEEECEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
THAFYIDEEADRPPWNGLTVPEDDPDEIDYTRGSESRYSWIKAPHYDGTPCEVGPISRLL
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHH
IMEEPLITGLANAFEENGYSPVNNYTRMLARMHEILLLIPEIMTWIVEDLTPNAKVAVHT
HHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ELSMARNSEGMGLWEAPRGALGHWVASGSNSMTTLYQAVVPSTWNLAPRNAQGIPSPVEQ
HHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
ALIGTKISAAENALGVDYSNPLGILHTARSYDPCLACAIHTIDKTEKNPDRIIRVV
HHHCCHHHHHCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCHHHEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]