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The map label for this gene is pyrF

Identifier: 21228185

GI number: 21228185

Start: 2482990

End: 2483652

Strand: Direct

Name: pyrF

Synonym: MM_2083

Alternate gene names: 21228185

Gene position: 2482990-2483652 (Clockwise)

Preceding gene: 21228184

Following gene: 21228192

Centisome position: 60.61

GC content: 49.32

Gene sequence:

>663_bases
ATGGAACGAAATACATGTATGATCCTTGCCCTTGATGTGACTGAGAGGGAAGAAGCGCTGAAGATTGCAGAAAATGTCCG
GGAATTTGTGGATGCAATAAAAGTGGGATACCCTCTAATTCTTGCCACCGGGCTTGATATTATTAGAGAGCTTGCCAGAT
TTGCCCCGGTCATAGCTGACTTCAAGGTTGCAGATATCCCTAACACCAACCGTCTTATCTGCGAACAGGTCTTTAAAGCC
GGAGCCGATGCGGTCATTGTGCAGGGCTTTACTGGCAGGGACAGCCTTGATGCCTGCATTGAAGTTGCTTCCAAATACGG
AAAAGATGTTTTTGTTGTAAGCGAAATGAGCCACCCGGGAGGAGCCGAGTTCCTGCAGTCAGCAGCAGAAGCGATTGCAA
AAATGGCAGTTGAAGCCGGAGCTTTCGGGCTTGTTGCTCCAGCCACACGTCCGGAAAGGGTAAAAGAGATAAGAAAAATT
ATCGGAGACAGGCTTACCATTATCTCTCCGGGTGTGGGTGCCCAGGGAGGAAAAGCCTCTGACGTTATCTCTGCAGGGGC
AGACTGGGTGATTGTAGGAAGAAGCATTTACAAGGCGGAATCTCCAAAAGAGGCTGCCTGCGAGATTGCTGAAGAAATTC
AGGCTGAGCTCCGCGGAAAGTAA

Upstream 100 bases:

>100_bases
GGGGAAGATGCAAAATCAGTTACCGTATATGCAGATTCAACAATTACTCTCCCGCTTATAGTCTCTGCTGTGAGGACGCG
CCTTTCAAAGAGGTGAGCTT

Downstream 100 bases:

>100_bases
TAAAGAATATTTTTGCTGAATGAATATTTTTTGGATTGATTGATTGATTCCATAGAAATTTTAAACCTAAAATACGTGCC
TGTGATGAAAAATCTGGCTG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MERNTCMILALDVTEREEALKIAENVREFVDAIKVGYPLILATGLDIIRELARFAPVIADFKVADIPNTNRLICEQVFKA
GADAVIVQGFTGRDSLDACIEVASKYGKDVFVVSEMSHPGGAEFLQSAAEAIAKMAVEAGAFGLVAPATRPERVKEIRKI
IGDRLTIISPGVGAQGGKASDVISAGADWVIVGRSIYKAESPKEAACEIAEEIQAELRGK

Sequences:

>Translated_220_residues
MERNTCMILALDVTEREEALKIAENVREFVDAIKVGYPLILATGLDIIRELARFAPVIADFKVADIPNTNRLICEQVFKA
GADAVIVQGFTGRDSLDACIEVASKYGKDVFVVSEMSHPGGAEFLQSAAEAIAKMAVEAGAFGLVAPATRPERVKEIRKI
IGDRLTIISPGVGAQGGKASDVISAGADWVIVGRSIYKAESPKEAACEIAEEIQAELRGK
>Mature_220_residues
MERNTCMILALDVTEREEALKIAENVREFVDAIKVGYPLILATGLDIIRELARFAPVIADFKVADIPNTNRLICEQVFKA
GADAVIVQGFTGRDSLDACIEVASKYGKDVFVVSEMSHPGGAEFLQSAAEAIAKMAVEAGAFGLVAPATRPERVKEIRKI
IGDRLTIISPGVGAQGGKASDVISAGADWVIVGRSIYKAESPKEAACEIAEEIQAELRGK

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=232, Percent_Identity=26.2931034482759, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_METMA (Q8PV88)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_634107.1
- ProteinModelPortal:   Q8PV88
- SMR:   Q8PV88
- GeneID:   1480425
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_2083
- NMPDR:   fig|192952.1.peg.2083
- HOGENOM:   HBG625253
- OMA:   TEMSHPG
- ProtClustDB:   PRK13813
- BioCyc:   MMAZ192952:MM2083-MONOMER
- BRENDA:   4.1.1.23
- HAMAP:   MF_01200_A
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 23503; Mature: 23503

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 62-62 BINDING 12-12 BINDING 34-34 BINDING 117-117 BINDING 193-193 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERNTCMILALDVTEREEALKIAENVREFVDAIKVGYPLILATGLDIIRELARFAPVIAD
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
FKVADIPNTNRLICEQVFKAGADAVIVQGFTGRDSLDACIEVASKYGKDVFVVSEMSHPG
CEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCC
GAEFLQSAAEAIAKMAVEAGAFGLVAPATRPERVKEIRKIIGDRLTIISPGVGAQGGKAS
HHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHH
DVISAGADWVIVGRSIYKAESPKEAACEIAEEIQAELRGK
HHHHCCCCEEEECCHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MERNTCMILALDVTEREEALKIAENVREFVDAIKVGYPLILATGLDIIRELARFAPVIAD
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
FKVADIPNTNRLICEQVFKAGADAVIVQGFTGRDSLDACIEVASKYGKDVFVVSEMSHPG
CEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCC
GAEFLQSAAEAIAKMAVEAGAFGLVAPATRPERVKEIRKIIGDRLTIISPGVGAQGGKAS
HHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCHH
DVISAGADWVIVGRSIYKAESPKEAACEIAEEIQAELRGK
HHHHCCCCEEEECCHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824