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The map label for this gene is cobS

Identifier: 21228159

GI number: 21228159

Start: 2451641

End: 2452483

Strand: Reverse

Name: cobS

Synonym: MM_2057

Alternate gene names: 21228159

Gene position: 2452483-2451641 (Counterclockwise)

Preceding gene: 21228160

Following gene: 21228158

Centisome position: 59.87

GC content: 44.84

Gene sequence:

>843_bases
ATGAATTCATATTTACTTGCATTTAAGTCAGGTTTTGGTTTTCTGTCCACGATCCCTGTAGGAATTACTATGGAAGGGAT
CGATGAGCTCATGAAAAAGATTTTTTTTTACCCTGTAGTTGGAGCCGTTCTCGGGCTTCTAATAGGGATAGTTGCATATG
CGGGACAGCTGGTATTTCCGGGTCCTGTTCTGGCAGCTCTTATTATGGGGTTCGTATATTACATCACAGGCTTTAACCAC
CTCGATGGAGTCACGGACATGGGAGACGGATTTATGGCCCATGGATCCCTTGAGAAAAAAGTCAAAGCCTTGAAAGACAC
AACCCTCGGGACAGGAGGAGTTGCTTTTGGTATACTTGTACTGCTTGCCTTTTACGGCTCTATAAGGTCAGTCCAGGAAG
AAGGAATTGCTGCATTTGGCTCTAATCTTCCGTTCCTGATGTTTGCATCTATGTTTATAGCGGAAGTCAGCGCCAAGCAG
TCCATGTTAACAATTGCAGCTTTTGGAAAACCACTTCCACGGCTGAAGGAACAAACTTATCCTGGCCTCGGGGAGATGAC
AATAAACGGAGCAACCAGGAAAAATTTTCTTATAGGATTTATTTTCGGTGCGGTTGTATGCTGCCTGCCCTTTGGACTGA
TAGGGCTTATTCCATACCTGGCAGCATGCATCTCTGCTCTCGTACTCCTTAACAGGAGTTATGCACACTTTGGTGGCTTG
AACGGTGACGGAATCGGTACAGCCAACGAGATTGGGAGGATAACTGCCCTGATTGTAATCGCAGTTACCCTTAAACTTTC
ATTAAATGGATACCTTGGAGGTTTAGAATGGACGCTATTGTAA

Upstream 100 bases:

>100_bases
ACGATGTCATAGGAGGCCTCATAGGAGGGGTGTCCTCCAATATGTATTCAAGAGGGATGGCAGAGTTTGAAAGAAAAGAC
TGAAAAACAGGCGGTACATA

Downstream 100 bases:

>100_bases
TGGCAGGAGGGTTCGGGCAGAGGCTTGGTATGGGGGAAAAGCCCTGCGTAGAATTGCTCGGAAAGCCGCTTATTACCTAT
GTAATAGATAACCTTAGAGC

Product: cobalamin [5'-phosphate] synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MNSYLLAFKSGFGFLSTIPVGITMEGIDELMKKIFFYPVVGAVLGLLIGIVAYAGQLVFPGPVLAALIMGFVYYITGFNH
LDGVTDMGDGFMAHGSLEKKVKALKDTTLGTGGVAFGILVLLAFYGSIRSVQEEGIAAFGSNLPFLMFASMFIAEVSAKQ
SMLTIAAFGKPLPRLKEQTYPGLGEMTINGATRKNFLIGFIFGAVVCCLPFGLIGLIPYLAACISALVLLNRSYAHFGGL
NGDGIGTANEIGRITALIVIAVTLKLSLNGYLGGLEWTLL

Sequences:

>Translated_280_residues
MNSYLLAFKSGFGFLSTIPVGITMEGIDELMKKIFFYPVVGAVLGLLIGIVAYAGQLVFPGPVLAALIMGFVYYITGFNH
LDGVTDMGDGFMAHGSLEKKVKALKDTTLGTGGVAFGILVLLAFYGSIRSVQEEGIAAFGSNLPFLMFASMFIAEVSAKQ
SMLTIAAFGKPLPRLKEQTYPGLGEMTINGATRKNFLIGFIFGAVVCCLPFGLIGLIPYLAACISALVLLNRSYAHFGGL
NGDGIGTANEIGRITALIVIAVTLKLSLNGYLGGLEWTLL
>Mature_280_residues
MNSYLLAFKSGFGFLSTIPVGITMEGIDELMKKIFFYPVVGAVLGLLIGIVAYAGQLVFPGPVLAALIMGFVYYITGFNH
LDGVTDMGDGFMAHGSLEKKVKALKDTTLGTGGVAFGILVLLAFYGSIRSVQEEGIAAFGSNLPFLMFASMFIAEVSAKQ
SMLTIAAFGKPLPRLKEQTYPGLGEMTINGATRKNFLIGFIFGAVVCCLPFGLIGLIPYLAACISALVLLNRSYAHFGGL
NGDGIGTANEIGRITALIVIAVTLKLSLNGYLGGLEWTLL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_METMA (Q8PVB4)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_634081.1
- GeneID:   1480399
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_2057
- NMPDR:   fig|192952.1.peg.2057
- HOGENOM:   HBG729392
- OMA:   INGATRK
- ProtClustDB:   CLSK568791
- BioCyc:   MMAZ192952:MM2057-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- InterPro:   IPR001411
- PRINTS:   PR01036
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 29619; Mature: 29619

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xf66e260)-; HASH(0xf59da18)-; HASH(0xc8ddb90)-; HASH(0xf0d5cc0)-; HASH(0xff1edd4)-; HASH(0xff40618)-; HASH(0xfa7c7e8)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSYLLAFKSGFGFLSTIPVGITMEGIDELMKKIFFYPVVGAVLGLLIGIVAYAGQLVFP
CCCEEEEEECCCCHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GPVLAALIMGFVYYITGFNHLDGVTDMGDGFMAHGSLEKKVKALKDTTLGTGGVAFGILV
HHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEECCCHHHHHHHHHHCCCCCCHHHHHHHH
LLAFYGSIRSVQEEGIAAFGSNLPFLMFASMFIAEVSAKQSMLTIAAFGKPLPRLKEQTY
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
PGLGEMTINGATRKNFLIGFIFGAVVCCLPFGLIGLIPYLAACISALVLLNRSYAHFGGL
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
NGDGIGTANEIGRITALIVIAVTLKLSLNGYLGGLEWTLL
CCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEH
>Mature Secondary Structure
MNSYLLAFKSGFGFLSTIPVGITMEGIDELMKKIFFYPVVGAVLGLLIGIVAYAGQLVFP
CCCEEEEEECCCCHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GPVLAALIMGFVYYITGFNHLDGVTDMGDGFMAHGSLEKKVKALKDTTLGTGGVAFGILV
HHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCEECCCHHHHHHHHHHCCCCCCHHHHHHHH
LLAFYGSIRSVQEEGIAAFGSNLPFLMFASMFIAEVSAKQSMLTIAAFGKPLPRLKEQTY
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
PGLGEMTINGATRKNFLIGFIFGAVVCCLPFGLIGLIPYLAACISALVLLNRSYAHFGGL
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
NGDGIGTANEIGRITALIVIAVTLKLSLNGYLGGLEWTLL
CCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12125824