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The map label for this gene is hisH [H]

Identifier: 21228132

GI number: 21228132

Start: 2425954

End: 2426577

Strand: Direct

Name: hisH [H]

Synonym: MM_2030

Alternate gene names: 21228132

Gene position: 2425954-2426577 (Clockwise)

Preceding gene: 21228131

Following gene: 21228133

Centisome position: 59.22

GC content: 47.12

Gene sequence:

>624_bases
TTGTTTGTTAGAGCTATGAAAAGAATCGTGATTATCGATTACGGGCTTGGAAATCTCAGAAGTGTTCAAAAAGGGCTTGA
ACACGCCGGAGCAAGTCCCGCAATCTCCGGGAATCCTGAAGAGATCCTGGCAGCAGACGGTATTATTCTCCCAGGAGTCG
GTGCTTTTATAGATGCAATGAAGTGTCTGGTCCCTCTCAAAAAGACCATAGCCGAATTTGCAGAATCGGGAAAGCCAATG
CTTGGAATATGCCTGGGGCAGCAGGTTTTGATGAGCTCTTCCGAAGAAGGCAGGCTGACAGACGGGCTTGACCTCATTCA
GGGCAGGGTGCTTCGATTCCCGAAGTCTGAACTGAAAGTTCCTCAAATGGGCTGGAATAACATAAGGGTCAAACAGGACC
ACCCTCTGTTTAAAGGCATTCCCGACGGTTCTTTCGTGTATTTCGTGCACTCATATTATGTGGATACTGCTGCAGAGAAC
ACCCTTGCATCATGCGAATATGGGCTTGAATATGCGGCTTCAGTTGTAAACTCAAAAGGAAATGTTATGGGCACCCAGTT
CCACCCTGAAAAAAGCGGAGCCACCGGGTTAAAGATTTTGAGAAACTTCGTTGAAATGTGCTGA

Upstream 100 bases:

>100_bases
ATATTTTTCAGGTATATTTTCTATTTTTCTTTGATTCTTATTTCCTGATTTTTCTTGCCTTTCTGAAATTCTTTGTTCGT
AAGCTTAATCTATCTTAACT

Downstream 100 bases:

>100_bases
ATATTTTAAACTATAAATCTACTGGCTTGCTTTGTCTTTGCGGTTGAACATTTATTTATCATTTTTTAAACTTTTATGTC
TGTGTTTTGGGCATTTTGTA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MFVRAMKRIVIIDYGLGNLRSVQKGLEHAGASPAISGNPEEILAADGIILPGVGAFIDAMKCLVPLKKTIAEFAESGKPM
LGICLGQQVLMSSSEEGRLTDGLDLIQGRVLRFPKSELKVPQMGWNNIRVKQDHPLFKGIPDGSFVYFVHSYYVDTAAEN
TLASCEYGLEYAASVVNSKGNVMGTQFHPEKSGATGLKILRNFVEMC

Sequences:

>Translated_207_residues
MFVRAMKRIVIIDYGLGNLRSVQKGLEHAGASPAISGNPEEILAADGIILPGVGAFIDAMKCLVPLKKTIAEFAESGKPM
LGICLGQQVLMSSSEEGRLTDGLDLIQGRVLRFPKSELKVPQMGWNNIRVKQDHPLFKGIPDGSFVYFVHSYYVDTAAEN
TLASCEYGLEYAASVVNSKGNVMGTQFHPEKSGATGLKILRNFVEMC
>Mature_207_residues
MFVRAMKRIVIIDYGLGNLRSVQKGLEHAGASPAISGNPEEILAADGIILPGVGAFIDAMKCLVPLKKTIAEFAESGKPM
LGICLGQQVLMSSSEEGRLTDGLDLIQGRVLRFPKSELKVPQMGWNNIRVKQDHPLFKGIPDGSFVYFVHSYYVDTAAEN
TLASCEYGLEYAASVVNSKGNVMGTQFHPEKSGATGLKILRNFVEMC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=198, Percent_Identity=41.4141414141414, Blast_Score=147, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=35.981308411215, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22466; Mature: 22466

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVRAMKRIVIIDYGLGNLRSVQKGLEHAGASPAISGNPEEILAADGIILPGVGAFIDAM
CHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCEEECCHHHHHHHH
KCLVPLKKTIAEFAESGKPMLGICLGQQVLMSSSEEGRLTDGLDLIQGRVLRFPKSELKV
HHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCC
PQMGWNNIRVKQDHPLFKGIPDGSFVYFVHSYYVDTAAENTLASCEYGLEYAASVVNSKG
CCCCCCCEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NVMGTQFHPEKSGATGLKILRNFVEMC
CEEECCCCCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MFVRAMKRIVIIDYGLGNLRSVQKGLEHAGASPAISGNPEEILAADGIILPGVGAFIDAM
CHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCEEECCHHHHHHHH
KCLVPLKKTIAEFAESGKPMLGICLGQQVLMSSSEEGRLTDGLDLIQGRVLRFPKSELKV
HHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCC
PQMGWNNIRVKQDHPLFKGIPDGSFVYFVHSYYVDTAAENTLASCEYGLEYAASVVNSKG
CCCCCCCEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NVMGTQFHPEKSGATGLKILRNFVEMC
CEEECCCCCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]