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The map label for this gene is iscS [H]

Identifier: 21228057

GI number: 21228057

Start: 2324969

End: 2326123

Strand: Reverse

Name: iscS [H]

Synonym: MM_1955

Alternate gene names: 21228057

Gene position: 2326123-2324969 (Counterclockwise)

Preceding gene: 21228058

Following gene: 21228056

Centisome position: 56.79

GC content: 47.88

Gene sequence:

>1155_bases
ATGATTTATCTAGACAATTCTGCATGCACAAGGCTGGACGAGAGAGTGCTTGAAGCAATGAAGCCCTATTTTTTTGATAC
TTATGCAGTAGCGACATCCGAATTTGGCTATTCCATGGGAATTGACGCAAAAGAAGGGCTTGAAAACTCCAGGGAAATCA
TAGCCTCAAGGCTTGGTGCGGACTCCGAAGAAATAATATTCACGTCGGGAGATACGGAATCAAGCAACATGGCATTGAAA
GGGACAGCCTGGGCACTTAAGGAGAAAAAAGGCAGGCATATTATTGTTCTGCAGATAGAGGATTTTCCGGTGTTGAATAC
GGCAAAATCCCTCCAGAAACAGGGTTTTGATGTCACATTTCTGAATGTTGACGGGGAAGGGTTTGCAGACCTTGAAGGGC
TCAGAAAAGCAATTACAAAAGAAACTGTTCTTGTCTCAATCCAGTACGCCAACCAGGAGATAGGGACGGTCCAGGACCTG
AAGGCAATCTCTGAGATCTGCAAAGAAAAAGATGTCCTTCTGCATACCGATGCCACACACAGTTTTACCCGGCTTCCCCT
GAATGTAAAAGAACTGCCTGTTGACCTGGTTACATTGTCAGCCCACACGATTCACGGTCCCAGGGGAATAGGTGCTCTCT
ACATCAGAAAAGGCACTCCAGTAAGCAAATTCATGGATGGCGGCTTTCAGGAGTTCAATTTGAGGGCAGGGGTTGAAAAT
ATTCCCGGAGCCGCAGGTTTTGCAACAGCAGTAGAACTTGTAACCGAAGATGAGACCAGGCAGCTCGAAGCCATGAGGAA
CAGGGTAATTGAGAGAGCCCTCTCTGAAATTCCGGATGTCACTCTTAACGGGAGCCGGGAAAAACGCCTGCCTCAGAATG
CAAACCTGACTTTCCATTATGTGGAAGGAGAATCCATAACCCTGCATATGGACATGAGAGGGTTTGCGGTGAGCACAGGT
TCAGCCTGTTTCAGCCGTTCCCTTGAAGCCAGCCATGTTATAAGGGGGATAGGAGGGGACCATGAAAGAGCTCATGGTTC
GGTTCGCTTCACTTTCGGACGTTACAACCGCATGGAAGATGCGGACGCTGCAGTTGATGCCATGAGTGAGGTTGTGGCAA
GGCTGAGGGAAATTAGCCCGCTTGCAAGAAAATAA

Upstream 100 bases:

>100_bases
ACAGCTTTACTGAGGAGATGAAGTATTAATTAAAAACAGAAACTGAGTCACGGGAATTAAATTTTATATGATCTGAGGAG
GGGGAAAAATATTTGAAAAT

Downstream 100 bases:

>100_bases
GCAGAAGGAAAAATGAAGAGAAGAGAAAAAGCAGCGGAAGAATAGAAAAATAAAGGGGAGAGCAGGATGAAAATCGAAAA
GAAAAGTGAGATGAAGTTTC

Product: cysteine desulfurase NifS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MIYLDNSACTRLDERVLEAMKPYFFDTYAVATSEFGYSMGIDAKEGLENSREIIASRLGADSEEIIFTSGDTESSNMALK
GTAWALKEKKGRHIIVLQIEDFPVLNTAKSLQKQGFDVTFLNVDGEGFADLEGLRKAITKETVLVSIQYANQEIGTVQDL
KAISEICKEKDVLLHTDATHSFTRLPLNVKELPVDLVTLSAHTIHGPRGIGALYIRKGTPVSKFMDGGFQEFNLRAGVEN
IPGAAGFATAVELVTEDETRQLEAMRNRVIERALSEIPDVTLNGSREKRLPQNANLTFHYVEGESITLHMDMRGFAVSTG
SACFSRSLEASHVIRGIGGDHERAHGSVRFTFGRYNRMEDADAAVDAMSEVVARLREISPLARK

Sequences:

>Translated_384_residues
MIYLDNSACTRLDERVLEAMKPYFFDTYAVATSEFGYSMGIDAKEGLENSREIIASRLGADSEEIIFTSGDTESSNMALK
GTAWALKEKKGRHIIVLQIEDFPVLNTAKSLQKQGFDVTFLNVDGEGFADLEGLRKAITKETVLVSIQYANQEIGTVQDL
KAISEICKEKDVLLHTDATHSFTRLPLNVKELPVDLVTLSAHTIHGPRGIGALYIRKGTPVSKFMDGGFQEFNLRAGVEN
IPGAAGFATAVELVTEDETRQLEAMRNRVIERALSEIPDVTLNGSREKRLPQNANLTFHYVEGESITLHMDMRGFAVSTG
SACFSRSLEASHVIRGIGGDHERAHGSVRFTFGRYNRMEDADAAVDAMSEVVARLREISPLARK
>Mature_384_residues
MIYLDNSACTRLDERVLEAMKPYFFDTYAVATSEFGYSMGIDAKEGLENSREIIASRLGADSEEIIFTSGDTESSNMALK
GTAWALKEKKGRHIIVLQIEDFPVLNTAKSLQKQGFDVTFLNVDGEGFADLEGLRKAITKETVLVSIQYANQEIGTVQDL
KAISEICKEKDVLLHTDATHSFTRLPLNVKELPVDLVTLSAHTIHGPRGIGALYIRKGTPVSKFMDGGFQEFNLRAGVEN
IPGAAGFATAVELVTEDETRQLEAMRNRVIERALSEIPDVTLNGSREKRLPQNANLTFHYVEGESITLHMDMRGFAVSTG
SACFSRSLEASHVIRGIGGDHERAHGSVRFTFGRYNRMEDADAAVDAMSEVVARLREISPLARK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=385, Percent_Identity=40.5194805194805, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI156713448, Length=415, Percent_Identity=29.8795180722892, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI48994898, Length=390, Percent_Identity=41.025641025641, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1789175, Length=395, Percent_Identity=26.0759493670886, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787970, Length=394, Percent_Identity=23.3502538071066, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI25143064, Length=385, Percent_Identity=41.038961038961, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI17533177, Length=329, Percent_Identity=29.1793313069909, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319831, Length=385, Percent_Identity=41.5584415584416, Blast_Score=288, Evalue=1e-78,
Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=41.8181818181818, Blast_Score=291, Evalue=6e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42367; Mature: 42367

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNSACTRLDERVLEAMKPYFFDTYAVATSEFGYSMGIDAKEGLENSREIIASRLGA
CEEECCHHHHHHHHHHHHHHCCHHEEHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHCC
DSEEIIFTSGDTESSNMALKGTAWALKEKKGRHIIVLQIEDFPVLNTAKSLQKQGFDVTF
CCCEEEEECCCCCCCCEEEEEHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEE
LNVDGEGFADLEGLRKAITKETVLVSIQYANQEIGTVQDLKAISEICKEKDVLLHTDATH
EEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
SFTRLPLNVKELPVDLVTLSAHTIHGPRGIGALYIRKGTPVSKFMDGGFQEFNLRAGVEN
CEEECCCCHHHCCEEEEEEEEEEECCCCCCCEEEEECCCCHHHHHCCCCEEEEEECCCCC
IPGAAGFATAVELVTEDETRQLEAMRNRVIERALSEIPDVTLNGSREKRLPQNANLTFHY
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHCCCCCCCEEEEE
VEGESITLHMDMRGFAVSTGSACFSRSLEASHVIRGIGGDHERAHGSVRFTFGRYNRMED
ECCCEEEEEEECCCEEEECCHHHHHCCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCH
ADAAVDAMSEVVARLREISPLARK
HHHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure
MIYLDNSACTRLDERVLEAMKPYFFDTYAVATSEFGYSMGIDAKEGLENSREIIASRLGA
CEEECCHHHHHHHHHHHHHHCCHHEEHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHCC
DSEEIIFTSGDTESSNMALKGTAWALKEKKGRHIIVLQIEDFPVLNTAKSLQKQGFDVTF
CCCEEEEECCCCCCCCEEEEEHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEE
LNVDGEGFADLEGLRKAITKETVLVSIQYANQEIGTVQDLKAISEICKEKDVLLHTDATH
EEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
SFTRLPLNVKELPVDLVTLSAHTIHGPRGIGALYIRKGTPVSKFMDGGFQEFNLRAGVEN
CEEECCCCHHHCCEEEEEEEEEEECCCCCCCEEEEECCCCHHHHHCCCCEEEEEECCCCC
IPGAAGFATAVELVTEDETRQLEAMRNRVIERALSEIPDVTLNGSREKRLPQNANLTFHY
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHCCCCCCCEEEEE
VEGESITLHMDMRGFAVSTGSACFSRSLEASHVIRGIGGDHERAHGSVRFTFGRYNRMED
ECCCEEEEEEECCCEEEECCHHHHHCCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCH
ADAAVDAMSEVVARLREISPLARK
HHHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA