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The map label for this gene is nifU [H]

Identifier: 21228056

GI number: 21228056

Start: 2324243

End: 2324902

Strand: Reverse

Name: nifU [H]

Synonym: MM_1954

Alternate gene names: 21228056

Gene position: 2324902-2324243 (Counterclockwise)

Preceding gene: 21228057

Following gene: 21228053

Centisome position: 56.76

GC content: 47.73

Gene sequence:

>660_bases
ATGAAAATCGAAAAGAAAAGTGAGATGAAGTTTCCTTATAGTGAAAAAGTTCTTGAGCATTTCAGGAACCCGCATAATGT
GGGGAAGATAGAGAACCCTGACGGAAAAGGCCTGGAAGGAAGCCCTGCATGCGGGGATATGGTTGCAGTATACATTAAGG
TCGACCCCAGGACAAAGGTCATTGAGGATATAAAATTCGAATCTTACGGATGTGCATCCAACATTGCCACTGGGTCCGTA
ATCACAGACCTTGCACGTGGAAAGACCCTGGATGAAGCTAAAAAAATAACCTGGAAACAGGCTTCAGAAGAGCTTGATGG
CCTTCCTCCGATAAAAGCCCACTGTTCCGTGCTTGCTGTTGAGGGCCTGCGTGCCGCAATCCGGGACTACGAAGAAAAAC
ACGGGCTCGTCACAGAGAAAGAACCGACGACGGAAGAGGTTGTCCAGCAGAGGCTCAAACACGTTATGAACCCTCTGACA
GGTCTTGATGTTGTTCGCACCAACCTTATACTTAAAACGAGTGTTGAAGCAGGAGTTATCAGGGTGGTTGTTGACCTGCC
CGCAGACCACCAGTTTGCTCCGGCAATAAAAGAGGATATAATTGAAAAGCTCGGGGCTCTCTGGGATGTGGAAAGAGTAG
ATGTGGTGTTTACAGCCTGA

Upstream 100 bases:

>100_bases
GCTGAGGGAAATTAGCCCGCTTGCAAGAAAATAAGCAGAAGGAAAAATGAAGAGAAGAGAAAAAGCAGCGGAAGAATAGA
AAAATAAAGGGGAGAGCAGG

Downstream 100 bases:

>100_bases
ATCATGCCTTAAGCAGAGAGAACAGGGAGAAAATAATTAATGGAAGCGGTTTATGAAGCCGATACTGGAGTTCAGCCCGG
ATAAACATTCAAAATACGTT

Product: NifU protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKIEKKSEMKFPYSEKVLEHFRNPHNVGKIENPDGKGLEGSPACGDMVAVYIKVDPRTKVIEDIKFESYGCASNIATGSV
ITDLARGKTLDEAKKITWKQASEELDGLPPIKAHCSVLAVEGLRAAIRDYEEKHGLVTEKEPTTEEVVQQRLKHVMNPLT
GLDVVRTNLILKTSVEAGVIRVVVDLPADHQFAPAIKEDIIEKLGALWDVERVDVVFTA

Sequences:

>Translated_219_residues
MKIEKKSEMKFPYSEKVLEHFRNPHNVGKIENPDGKGLEGSPACGDMVAVYIKVDPRTKVIEDIKFESYGCASNIATGSV
ITDLARGKTLDEAKKITWKQASEELDGLPPIKAHCSVLAVEGLRAAIRDYEEKHGLVTEKEPTTEEVVQQRLKHVMNPLT
GLDVVRTNLILKTSVEAGVIRVVVDLPADHQFAPAIKEDIIEKLGALWDVERVDVVFTA
>Mature_219_residues
MKIEKKSEMKFPYSEKVLEHFRNPHNVGKIENPDGKGLEGSPACGDMVAVYIKVDPRTKVIEDIKFESYGCASNIATGSV
ITDLARGKTLDEAKKITWKQASEELDGLPPIKAHCSVLAVEGLRAAIRDYEEKHGLVTEKEPTTEEVVQQRLKHVMNPLT
GLDVVRTNLILKTSVEAGVIRVVVDLPADHQFAPAIKEDIIEKLGALWDVERVDVVFTA

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI24307953, Length=130, Percent_Identity=40.7692307692308, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI56699456, Length=125, Percent_Identity=42.4, Blast_Score=108, Evalue=4e-24,
Organism=Escherichia coli, GI1788878, Length=127, Percent_Identity=48.0314960629921, Blast_Score=127, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17543474, Length=125, Percent_Identity=45.6, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6325122, Length=129, Percent_Identity=48.062015503876, Blast_Score=126, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324800, Length=124, Percent_Identity=45.1612903225806, Blast_Score=122, Evalue=5e-29,
Organism=Drosophila melanogaster, GI21355597, Length=125, Percent_Identity=45.6, Blast_Score=117, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002871 [H]

Pfam domain/function: PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 24255; Mature: 24255

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEKKSEMKFPYSEKVLEHFRNPHNVGKIENPDGKGLEGSPACGDMVAVYIKVDPRTKV
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHH
IEDIKFESYGCASNIATGSVITDLARGKTLDEAKKITWKQASEELDGLPPIKAHCSVLAV
HHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EGLRAAIRDYEEKHGLVTEKEPTTEEVVQQRLKHVMNPLTGLDVVRTNLILKTSVEAGVI
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCEE
RVVVDLPADHQFAPAIKEDIIEKLGALWDVERVDVVFTA
EEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MKIEKKSEMKFPYSEKVLEHFRNPHNVGKIENPDGKGLEGSPACGDMVAVYIKVDPRTKV
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHH
IEDIKFESYGCASNIATGSVITDLARGKTLDEAKKITWKQASEELDGLPPIKAHCSVLAV
HHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EGLRAAIRDYEEKHGLVTEKEPTTEEVVQQRLKHVMNPLTGLDVVRTNLILKTSVEAGVI
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCEE
RVVVDLPADHQFAPAIKEDIIEKLGALWDVERVDVVFTA
EEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]