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The map label for this gene is mtbA [H]

Identifier: 21228034

GI number: 21228034

Start: 2300198

End: 2301223

Strand: Direct

Name: mtbA [H]

Synonym: MM_1932

Alternate gene names: 21228034

Gene position: 2300198-2301223 (Clockwise)

Preceding gene: 21228033

Following gene: 21228036

Centisome position: 56.15

GC content: 48.05

Gene sequence:

>1026_bases
ATGAGAGAATTAACCCTTAACACGAGATTTAAACGGGCGCTGAAAGGTGAATCTATTGACATGGTTCCTGTCTGTTCTGT
TACCCAGACTGGCACGGTAGAACTTATGGAAATGACTGGAGCATACTGGCCTCAGGCTAATTACGATGCAGCTAAAATGG
CTGCACTTGCTCTGGGTGGATACGAGATCGCAGGATTTGAGAATGTGCGGTGCCCTTTTTGCACCACAGTGCTTGCTGAA
ACTCTGGGATGTACGGTAGACGAAGGCAGCGTTGATATACAGCCTTATGTAACAGATTTCCCCTGCAAGAAAAAGAAAGA
CGTTAAGGACATCTCAGTTCCTGATTCTCTTCTTGAAAGCAGGTTGACATCCGTAGTGCTGGACGCGGTTGAAATCCTCA
AAGAACGTGTGGGGGAGGATGTTCCTGTTGTCGCAGGGGTTGTAGGTCCTGCCGGGCTTGCTTCCATGCTTGCGGGAATG
AGAAACTACCTCATGTGGTTTGTAACAAACCCCGAAGTCGTTGAAGAGCTCATGGGAACCGTAATGGAAGCCTGTATCGA
ATATGCGAACGGATTAATTGAAAGAGGTGCAGATGCCGTGACCCTGATAGATTCTGAAGCCGGACCTGATATCATTGCCC
CTCAGATGTTTGAGACCTCGGTTTTTCCGCTCTACAGGAAATTCTGCAAAGAAGTCAAAGGCTTGAAAATCCTGCATATG
TGTGGAGATGCAACGGCTGTCCTTGATCCTCTTGCAGACTCAGGATTTGAGGGAATCAGTATTGAAGAAAAAGTAGAGGT
CAGTTTTGCAAAGCGGATTGTGGGCGACCGGATACGTCTGATAGGAAATGTTTCCCCTTCGGATACCCTGTTGATGAAAG
GGACTGAAGAGGTTATTATTGAAGCCAGGGCGTGCCTTGAAGATGGAATCGATATCCTTGCACCCGGCTGTGGCCTTGCT
CCCCACACTCCACTCGGGAACATAAAAGCACTTCTCAGGGCAAGAGACGAGTACTGTTCACTCTGA

Upstream 100 bases:

>100_bases
TTCCCGGTATATTATAAAATAACAAATATTTCCAGGACATTATTTATCAGGCTGGCAGCCGAAACTGAACTATCAATATC
TGATTATAGCTGGTGAAAAT

Downstream 100 bases:

>100_bases
ACTTTCATTAGTGGCTGCGGTAAGGGGGGAGTTCTTTCAAATAAAAACTCTTTCTCCTGTTTTTTCCGAAATATCCTCGG
GCCCCTTAAGGCTTATGATC

Product: methylcobalamin:coenzyme M methyltransferase

Products: CO2; Coproporphyrinogen III [C]

Alternate protein names: MT2-A; Methylcobamide:CoM methyltransferase II isozyme A [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MRELTLNTRFKRALKGESIDMVPVCSVTQTGTVELMEMTGAYWPQANYDAAKMAALALGGYEIAGFENVRCPFCTTVLAE
TLGCTVDEGSVDIQPYVTDFPCKKKKDVKDISVPDSLLESRLTSVVLDAVEILKERVGEDVPVVAGVVGPAGLASMLAGM
RNYLMWFVTNPEVVEELMGTVMEACIEYANGLIERGADAVTLIDSEAGPDIIAPQMFETSVFPLYRKFCKEVKGLKILHM
CGDATAVLDPLADSGFEGISIEEKVEVSFAKRIVGDRIRLIGNVSPSDTLLMKGTEEVIIEARACLEDGIDILAPGCGLA
PHTPLGNIKALLRARDEYCSL

Sequences:

>Translated_341_residues
MRELTLNTRFKRALKGESIDMVPVCSVTQTGTVELMEMTGAYWPQANYDAAKMAALALGGYEIAGFENVRCPFCTTVLAE
TLGCTVDEGSVDIQPYVTDFPCKKKKDVKDISVPDSLLESRLTSVVLDAVEILKERVGEDVPVVAGVVGPAGLASMLAGM
RNYLMWFVTNPEVVEELMGTVMEACIEYANGLIERGADAVTLIDSEAGPDIIAPQMFETSVFPLYRKFCKEVKGLKILHM
CGDATAVLDPLADSGFEGISIEEKVEVSFAKRIVGDRIRLIGNVSPSDTLLMKGTEEVIIEARACLEDGIDILAPGCGLA
PHTPLGNIKALLRARDEYCSL
>Mature_341_residues
MRELTLNTRFKRALKGESIDMVPVCSVTQTGTVELMEMTGAYWPQANYDAAKMAALALGGYEIAGFENVRCPFCTTVLAE
TLGCTVDEGSVDIQPYVTDFPCKKKKDVKDISVPDSLLESRLTSVVLDAVEILKERVGEDVPVVAGVVGPAGLASMLAGM
RNYLMWFVTNPEVVEELMGTVMEACIEYANGLIERGADAVTLIDSEAGPDIIAPQMFETSVFPLYRKFCKEVKGLKILHM
CGDATAVLDPLADSGFEGISIEEKVEVSFAKRIVGDRIRLIGNVSPSDTLLMKGTEEVIIEARACLEDGIDILAPGCGLA
PHTPLGNIKALLRARDEYCSL

Specific function: Catalyzes the transfer of the methyl group from the methylated corrinoid cofactor of mtmC, mtbC and mttC to coenzyme M [H]

COG id: COG0407

COG function: function code H; Uroporphyrinogen-III decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uroporphyrinogen decarboxylase family. MtbA/mtaA subfamily [H]

Homologues:

Organism=Homo sapiens, GI71051616, Length=243, Percent_Identity=24.2798353909465, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006360
- InterPro:   IPR000257 [H]

Pfam domain/function: PF01208 URO-D [H]

EC number: 4.1.1.37 [C]

Molecular weight: Translated: 36882; Mature: 36882

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRELTLNTRFKRALKGESIDMVPVCSVTQTGTVELMEMTGAYWPQANYDAAKMAALALGG
CCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHCC
YEIAGFENVRCPFCTTVLAETLGCTVDEGSVDIQPYVTDFPCKKKKDVKDISVPDSLLES
EEECCCCCCCCHHHHHHHHHHHCCEECCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
RLTSVVLDAVEILKERVGEDVPVVAGVVGPAGLASMLAGMRNYLMWFVTNPEVVEELMGT
HHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHH
VMEACIEYANGLIERGADAVTLIDSEAGPDIIAPQMFETSVFPLYRKFCKEVKGLKILHM
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
CGDATAVLDPLADSGFEGISIEEKVEVSFAKRIVGDRIRLIGNVSPSDTLLMKGTEEVII
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCEEECCHHHHHH
EARACLEDGIDILAPGCGLAPHTPLGNIKALLRARDEYCSL
HHHHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRELTLNTRFKRALKGESIDMVPVCSVTQTGTVELMEMTGAYWPQANYDAAKMAALALGG
CCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHCC
YEIAGFENVRCPFCTTVLAETLGCTVDEGSVDIQPYVTDFPCKKKKDVKDISVPDSLLES
EEECCCCCCCCHHHHHHHHHHHCCEECCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
RLTSVVLDAVEILKERVGEDVPVVAGVVGPAGLASMLAGMRNYLMWFVTNPEVVEELMGT
HHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHH
VMEACIEYANGLIERGADAVTLIDSEAGPDIIAPQMFETSVFPLYRKFCKEVKGLKILHM
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
CGDATAVLDPLADSGFEGISIEEKVEVSFAKRIVGDRIRLIGNVSPSDTLLMKGTEEVII
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCEEECCHHHHHH
EARACLEDGIDILAPGCGLAPHTPLGNIKALLRARDEYCSL
HHHHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Reducing agents [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00231 {7-COOH,} 0.00053 {8-COOH-porphyrinogen} 0.00025 {8-COOH-porphyrinogen} 0.00533 {uroporphyrinogen} 0.00256 {uroporphyrinogen} 0.00133 {uroporphyrinogen} 0.001 {uroporphyrinogen} 0.0008 {uroporphyrinogen} 0.0004 {uroporphyrino

Substrates: H+; Uroporphyrinogen III [C]

Specific reaction: (4) H+ + Uroporphyrinogen III --> (4) CO2 + Coproporphyrinogen III [C]

General reaction: Decarboxylation [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); 7-COOH-porphyrinogenI; 7-COOH-porphyrinogen III; Butanedione; Diethyl dicarbonate; Fe2+; Iodoacetamide; p-Hydroxy mercuribenzoate; Phenyl glyoxal; Pt2+; Sn2+; UroporphyrinogenI uroporphyrinogen III; Uroporphyrinogen III;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]