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The map label for this gene is lig1

Identifier: 21227997

GI number: 21227997

Start: 2252874

End: 2254613

Strand: Reverse

Name: lig1

Synonym: MM_1895

Alternate gene names: 21227997

Gene position: 2254613-2252874 (Counterclockwise)

Preceding gene: 21227998

Following gene: 21227996

Centisome position: 55.04

GC content: 44.37

Gene sequence:

>1740_bases
GTGAGATTTAAAGAGCTTGCAGAGCTTTTTGAGGAGCTTGAGAAGACCACGTCCCACAGGGAAATCGTAAGAAAGATTTC
TGAATTTTTTAAGAATCTCAGGGGGGATGAAGTAAAGGACAGCGCGTATCTTTTTCTTGGAAGCACCGGGCCTGCCTTTG
AAAACACAACCCTCGGGATCAAGGACATGCTTGCCATACGGGCCATTGCAGGAGCTTACGGAGTTACCAGAGAAGATGTC
AGAAAAAGATATGCAAGAACAGGAGACCTAGGAGATGTGGCTTTTGAGCTCAGCAAAAAAAGAGAATCTTCCCTGACCAT
TGAGGATGTTTTTCAAAGGCTTCTGCAGATAAGAGAAACCTCAGGAAAAGGCAGTCAGGAAGAAAAAACAGCCCTTTTTT
CGGACATCTTACAAAAAGCCACACCTGAAGAAGGTAAATATATAGTGCGACTTGTGCTCGGAAGGTTAAGATTGGGGTTC
GGAGACCAGTTCTTGCTCGAAGCTTTCGCAATCGCATTTACAGGAGACAAAAAACATGCCGCAAAAATAAAAGAAAGCTA
TAGCGTCTGTACGGATATCGGGGAGCTAGCAAAAATCCTTGCAGAAAACGGAGCAAGGGCTACAGGGTTTATCTCCATAA
AACCGGGTAGGCCGGTAAAATCCATGCTCTCCCAGCGAGTCGAAAGTTTTGAAGAGCTTGAAAAAAGGGTTAAGGGAAAA
AAAGCTGCTGAAGAGAAATATGATGGAGAGAGGGTGCAGGTACATAAAACCGGTGAGGGGATTAAAGCTTTTTCCCGCAG
GCTTGAGGACATAACTTCCCAGTACCCTGAAATTATCGAAGATGTCAGAAAAACCGTCCCTGCAAATGAAATAGTGCTTG
ATGGAGAAATAGTTGCATACGCAGAACTGGAAAGAAACGGTAACCGGATAGAAGAGTTTTACCCGTTTCAGAACCTCATG
CAGAGGCGAAGAAAGTACGAGATTGAAAATTACAGGAAGAAATGCCCGGTAGCAGTATTCTTTTTTGATATCCTTTACCT
TAACGGAGAGCCCCTTCTAAAGAGGCCCTATCCTGAAAGAAGGGCTTTGCTTGAGATGAACGTTGTTGAGTCAGGAATAA
TTCGTCTGTCAAAAAGAATCGTTACGGAAAGCGTTGAGGAAATTGAGGATTTTTTTAATGAAACCATAGAAAAGGGGCTT
GAAGGAATAGTGGTCAAGTCCATGAGCAGCAACTCTTATTACGAAGCCGGGAAAAGGAGCTGGTTCTGGTTCAAATGGAA
ACAGGAGTATTCCGAGGGGATGAGAGAGACCTTTGACCTTGTGGTTGTGGGGAGTTATTACGGAAGAGGGAGGAGAAAAG
GGTCATTTGGAGCTCTTCTCTGTGCAGTCCTGAATAAAGAGGGACAGCGGTTTGAAACGTTGACAAAAGTGGGAACTGGT
TTTACAGAAGCGGATGCAGAAGAGATCAACAGGTTGCTTTCAGACCATATAGTAAGCGAAATTCCAAAGGGCGTTTCTAT
TAAAAAAGGTATGCTTCCTGATATCTTTATAGAACCTGCAGTAGTTATCGAAGTCCTTGGCTCGGAGATCACAAACAGCC
CCGGACATACGGCAGGAGAAGGAGAAGAAGAAACAGGACTTGCACTGCGTTTCCCCCGGTTCTTACGAATAAGGCACGAC
AAAACTCCATATGATGCCATGACAGTTAAGGAAGTAAGGGACCTGAAGGACGGAACTTAA

Upstream 100 bases:

>100_bases
AATCTCTTTTTTGGAAATTTGAGGGTGCAAAGCGATAAAGACCGGGGCAGGGAATAGTTATTGTAGAAATTTCAACCAGC
TTTTTCGGAGAGGGGCAATG

Downstream 100 bases:

>100_bases
AAAGTAAAAGAAAAGTGGAAGAGTGTAAGTAAAATATTTTGAAAATATCAGGAAAAATAACAGAGTGAGGGGGAGCCTTT
GAGAAACCGTGAGGTTGCAG

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] 1

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MRFKELAELFEELEKTTSHREIVRKISEFFKNLRGDEVKDSAYLFLGSTGPAFENTTLGIKDMLAIRAIAGAYGVTREDV
RKRYARTGDLGDVAFELSKKRESSLTIEDVFQRLLQIRETSGKGSQEEKTALFSDILQKATPEEGKYIVRLVLGRLRLGF
GDQFLLEAFAIAFTGDKKHAAKIKESYSVCTDIGELAKILAENGARATGFISIKPGRPVKSMLSQRVESFEELEKRVKGK
KAAEEKYDGERVQVHKTGEGIKAFSRRLEDITSQYPEIIEDVRKTVPANEIVLDGEIVAYAELERNGNRIEEFYPFQNLM
QRRRKYEIENYRKKCPVAVFFFDILYLNGEPLLKRPYPERRALLEMNVVESGIIRLSKRIVTESVEEIEDFFNETIEKGL
EGIVVKSMSSNSYYEAGKRSWFWFKWKQEYSEGMRETFDLVVVGSYYGRGRRKGSFGALLCAVLNKEGQRFETLTKVGTG
FTEADAEEINRLLSDHIVSEIPKGVSIKKGMLPDIFIEPAVVIEVLGSEITNSPGHTAGEGEEETGLALRFPRFLRIRHD
KTPYDAMTVKEVRDLKDGT

Sequences:

>Translated_579_residues
MRFKELAELFEELEKTTSHREIVRKISEFFKNLRGDEVKDSAYLFLGSTGPAFENTTLGIKDMLAIRAIAGAYGVTREDV
RKRYARTGDLGDVAFELSKKRESSLTIEDVFQRLLQIRETSGKGSQEEKTALFSDILQKATPEEGKYIVRLVLGRLRLGF
GDQFLLEAFAIAFTGDKKHAAKIKESYSVCTDIGELAKILAENGARATGFISIKPGRPVKSMLSQRVESFEELEKRVKGK
KAAEEKYDGERVQVHKTGEGIKAFSRRLEDITSQYPEIIEDVRKTVPANEIVLDGEIVAYAELERNGNRIEEFYPFQNLM
QRRRKYEIENYRKKCPVAVFFFDILYLNGEPLLKRPYPERRALLEMNVVESGIIRLSKRIVTESVEEIEDFFNETIEKGL
EGIVVKSMSSNSYYEAGKRSWFWFKWKQEYSEGMRETFDLVVVGSYYGRGRRKGSFGALLCAVLNKEGQRFETLTKVGTG
FTEADAEEINRLLSDHIVSEIPKGVSIKKGMLPDIFIEPAVVIEVLGSEITNSPGHTAGEGEEETGLALRFPRFLRIRHD
KTPYDAMTVKEVRDLKDGT
>Mature_579_residues
MRFKELAELFEELEKTTSHREIVRKISEFFKNLRGDEVKDSAYLFLGSTGPAFENTTLGIKDMLAIRAIAGAYGVTREDV
RKRYARTGDLGDVAFELSKKRESSLTIEDVFQRLLQIRETSGKGSQEEKTALFSDILQKATPEEGKYIVRLVLGRLRLGF
GDQFLLEAFAIAFTGDKKHAAKIKESYSVCTDIGELAKILAENGARATGFISIKPGRPVKSMLSQRVESFEELEKRVKGK
KAAEEKYDGERVQVHKTGEGIKAFSRRLEDITSQYPEIIEDVRKTVPANEIVLDGEIVAYAELERNGNRIEEFYPFQNLM
QRRRKYEIENYRKKCPVAVFFFDILYLNGEPLLKRPYPERRALLEMNVVESGIIRLSKRIVTESVEEIEDFFNETIEKGL
EGIVVKSMSSNSYYEAGKRSWFWFKWKQEYSEGMRETFDLVVVGSYYGRGRRKGSFGALLCAVLNKEGQRFETLTKVGTG
FTEADAEEINRLLSDHIVSEIPKGVSIKKGMLPDIFIEPAVVIEVLGSEITNSPGHTAGEGEEETGLALRFPRFLRIRHD
KTPYDAMTVKEVRDLKDGT

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family

Homologues:

Organism=Homo sapiens, GI4557719, Length=613, Percent_Identity=31.4845024469821, Blast_Score=292, Evalue=7e-79,
Organism=Homo sapiens, GI73747844, Length=607, Percent_Identity=28.171334431631, Blast_Score=193, Evalue=5e-49,
Organism=Homo sapiens, GI73747829, Length=607, Percent_Identity=28.171334431631, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI46255052, Length=624, Percent_Identity=27.4038461538462, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI23199993, Length=624, Percent_Identity=27.4038461538462, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI148539894, Length=624, Percent_Identity=27.4038461538462, Blast_Score=169, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI71985269, Length=605, Percent_Identity=32.0661157024793, Blast_Score=320, Evalue=9e-88,
Organism=Caenorhabditis elegans, GI71985278, Length=183, Percent_Identity=28.4153005464481, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320038, Length=613, Percent_Identity=31.810766721044, Blast_Score=291, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6324578, Length=620, Percent_Identity=25.4838709677419, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24762424, Length=607, Percent_Identity=31.1367380560132, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI85815841, Length=595, Percent_Identity=26.0504201680672, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24641829, Length=646, Percent_Identity=25.5417956656347, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLI1_METMA (Q8PVR7)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633919.1
- HSSP:   P18858
- ProteinModelPortal:   Q8PVR7
- SMR:   Q8PVR7
- GeneID:   1480237
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1895
- NMPDR:   fig|192952.1.peg.1895
- HOGENOM:   HBG570821
- OMA:   EYQSICK
- ProtClustDB:   CLSK281490
- BioCyc:   MMAZ192952:MM1895-MONOMER
- HAMAP:   MF_00407
- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- TIGRFAMs:   TIGR00574

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N; SSF50249 Nucleic_acid_OB

EC number: =6.5.1.1

Molecular weight: Translated: 65868; Mature: 65868

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00697 DNA_LIGASE_A1; PS00333 DNA_LIGASE_A2; PS50160 DNA_LIGASE_A3

Important sites: ACT_SITE 246-246 BINDING 244-244 BINDING 251-251 BINDING 266-266 BINDING 296-296 BINDING 342-342 BINDING 419-419 BINDING 425-425

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFKELAELFEELEKTTSHREIVRKISEFFKNLRGDEVKDSAYLFLGSTGPAFENTTLGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCH
KDMLAIRAIAGAYGVTREDVRKRYARTGDLGDVAFELSKKRESSLTIEDVFQRLLQIRET
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
SGKGSQEEKTALFSDILQKATPEEGKYIVRLVLGRLRLGFGDQFLLEAFAIAFTGDKKHA
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHH
AKIKESYSVCTDIGELAKILAENGARATGFISIKPGRPVKSMLSQRVESFEELEKRVKGK
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
KAAEEKYDGERVQVHKTGEGIKAFSRRLEDITSQYPEIIEDVRKTVPANEIVLDGEIVAY
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEE
AELERNGNRIEEFYPFQNLMQRRRKYEIENYRKKCPVAVFFFDILYLNGEPLLKRPYPER
EEHHCCCCHHHHHCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHH
RALLEMNVVESGIIRLSKRIVTESVEEIEDFFNETIEKGLEGIVVKSMSSNSYYEAGKRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCC
WFWFKWKQEYSEGMRETFDLVVVGSYYGRGRRKGSFGALLCAVLNKEGQRFETLTKVGTG
EEEEEHHHHHHHHHHHHHHEEEEECHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCC
FTEADAEEINRLLSDHIVSEIPKGVSIKKGMLPDIFIEPAVVIEVLGSEITNSPGHTAGE
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCC
GEEETGLALRFPRFLRIRHDKTPYDAMTVKEVRDLKDGT
CCCCCCCEEECHHHHHEECCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRFKELAELFEELEKTTSHREIVRKISEFFKNLRGDEVKDSAYLFLGSTGPAFENTTLGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCH
KDMLAIRAIAGAYGVTREDVRKRYARTGDLGDVAFELSKKRESSLTIEDVFQRLLQIRET
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
SGKGSQEEKTALFSDILQKATPEEGKYIVRLVLGRLRLGFGDQFLLEAFAIAFTGDKKHA
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHH
AKIKESYSVCTDIGELAKILAENGARATGFISIKPGRPVKSMLSQRVESFEELEKRVKGK
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
KAAEEKYDGERVQVHKTGEGIKAFSRRLEDITSQYPEIIEDVRKTVPANEIVLDGEIVAY
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEE
AELERNGNRIEEFYPFQNLMQRRRKYEIENYRKKCPVAVFFFDILYLNGEPLLKRPYPER
EEHHCCCCHHHHHCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHH
RALLEMNVVESGIIRLSKRIVTESVEEIEDFFNETIEKGLEGIVVKSMSSNSYYEAGKRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCC
WFWFKWKQEYSEGMRETFDLVVVGSYYGRGRRKGSFGALLCAVLNKEGQRFETLTKVGTG
EEEEEHHHHHHHHHHHHHHEEEEECHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCC
FTEADAEEINRLLSDHIVSEIPKGVSIKKGMLPDIFIEPAVVIEVLGSEITNSPGHTAGE
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCC
GEEETGLALRFPRFLRIRHDKTPYDAMTVKEVRDLKDGT
CCCCCCCEEECHHHHHEECCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824