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The map label for this gene is polX [H]

Identifier: 21227996

GI number: 21227996

Start: 2251041

End: 2252795

Strand: Reverse

Name: polX [H]

Synonym: MM_1894

Alternate gene names: 21227996

Gene position: 2252795-2251041 (Counterclockwise)

Preceding gene: 21227997

Following gene: 21227995

Centisome position: 55.0

GC content: 45.64

Gene sequence:

>1755_bases
TTGAGAAACCGTGAGGTTGCAGAACTGCTGTATGAAACTGCTGATATAATGGAGTTCCAGCAGATAGAGTGGAAACCGCG
AGCTTACAGGCGGGCAGCTCAGAACATAGAGAATTTTGGCGAAGATATTGAAAAAGTCTACGAGAAAAAAGGAAAAAAGG
GGCTTACAGAGATTCCCGGCGTAGGTGAATCCATTGCCGACCATATAGCCGAATACCTGAAAACAGGGAAGGTAGAAAAA
TTTGAGGGTTTGAAAGGGAAAGCTCCTTCAGGCACTGCCGAACTGATGGAAATCAGGGGGCTTGGAGCAAAAAAGATGAA
AAAGCTTGCAGATAAGCTTGAGATAAAAACCATGTCAGACCTGAAAAATGCGGTTAAATCTCACAGGATCAGGAGGCTTT
CAGGCTTCGGGGTAAAAAGTGAAGAAAATATCGCCAGGGCAATTGAAAACTACGAAAAAAGCCATTCAAGAATCCCGCTT
GGAAAGGCACTCCCGCTTGCCGAAGAAATCATATCCGGCTTGAAAGAAGAACTGAAGAACGGAACTCCCGGGTTAGACCT
CTCCAGGATCATCTATACAGGTTCCCTGCGAAGGCTGAAAGAAACTATTGGGGACATTGACATACTTGCAGAAGCAGAAG
AAGCAGATGCAGGGAAAATTATGGATGCATTTGTTTCTCTTCCCGAAGCAGGACAGGTTGTTTCAAAAGGCAGAACCAGA
AGCAGTGTGATCCTTAAAGAGGGATTTGCGATAGACCTCAGGGTAGTTCCGCCTGAAAGCTACGGGGCAGCTTTACAATA
TTTCACGGGCTCAAAGGAACACAATATCGGACTCAGAAATATTGCTCTCAGAGAAGGTTACAAGCTTTCAGAATACGGGC
TGTATTCCAAAAATTCGGGAGAACGGATTGCAGGCAAAAGTGAAGAAGAAATTTACAGGAAACTCGGGCTTGAGTATATC
GCGCCTGAACTCCGGGAAAACAGGGGAGAACTTAAGGCTGCAGCAAAGAATGAACTCCCAGAGCTTGTGGATGCAGGAGA
CCTCAGGGGAGACCTGCATATGCATACGGATTACAGCGAAGGGGCAGAGAGCCTTGAAACAATGGTAGAAAAAGCCGAAT
CTATGGGATACGAATATATTGCAGTCACAGACCATTCGCGCTCTCAGAGGATTGCAAACGGAATGGATATTGAAACTTTG
AAAGAGCAGTGGAAAGAGATCAAAAGTCTGTCAAAGCGCTTCAGGATAAAGGTTCTAAGGGGCTCGGAAGTTGAGATCCT
TAAAGACGGAAGCCTTGACTACCCTGATGAGATCCTTAAAGAGCTTGACGTTGTGGTAGGAGCTGTACATTCAGGTTTTG
CAGCATCGGAAAGAGAAATGACAGGAAGAATTATCACTGCTCTCGAAAACAAACACCTCGACATACTTGCCCATCCTTCG
GGCAGGCTTCTCGGAAAAAGAGAGGCTTATGCGGTCAATTTCGGGAAAGTCTTTGAAGCTGCAGCAGAAAATGGAAAAGT
AATGGAAATTAACTGCCAGCCTTCAAGGCTTGACCTTAATGACGAGCTTATCTTCAGGGCAAAGGACTATGAGCTGAAAT
TCTGCATTTCAACGGACAGCCACTCCGTATCTGACCTTGCTTCCATGCGTTACGGGCTCGGACAGGCAAGAAGGAGCTGG
CTTGAAAAAGAAGATATTGTGAATACCTACCCGTACTCCAGGCTTAAGGAGGTTTTCAAGAAACTCAGGGATTGA

Upstream 100 bases:

>100_bases
GGACCTGAAGGACGGAACTTAAAAAGTAAAAGAAAAGTGGAAGAGTGTAAGTAAAATATTTTGAAAATATCAGGAAAAAT
AACAGAGTGAGGGGGAGCCT

Downstream 100 bases:

>100_bases
AAGAAAAAATCAAAATTTTGTTGTTATTTTCTTGGAAAGGGATATTTTGTATTAAAATAGAGAAAAAATCTATTCTGGAT
ATAAATATCACAGAAAAGCC

Product: DNA polymerase beta

Products: NA

Alternate protein names: DNA polymerase type-X; 3'-5' exodeoxyribonuclease; 3'-5' exonuclease [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MRNREVAELLYETADIMEFQQIEWKPRAYRRAAQNIENFGEDIEKVYEKKGKKGLTEIPGVGESIADHIAEYLKTGKVEK
FEGLKGKAPSGTAELMEIRGLGAKKMKKLADKLEIKTMSDLKNAVKSHRIRRLSGFGVKSEENIARAIENYEKSHSRIPL
GKALPLAEEIISGLKEELKNGTPGLDLSRIIYTGSLRRLKETIGDIDILAEAEEADAGKIMDAFVSLPEAGQVVSKGRTR
SSVILKEGFAIDLRVVPPESYGAALQYFTGSKEHNIGLRNIALREGYKLSEYGLYSKNSGERIAGKSEEEIYRKLGLEYI
APELRENRGELKAAAKNELPELVDAGDLRGDLHMHTDYSEGAESLETMVEKAESMGYEYIAVTDHSRSQRIANGMDIETL
KEQWKEIKSLSKRFRIKVLRGSEVEILKDGSLDYPDEILKELDVVVGAVHSGFAASEREMTGRIITALENKHLDILAHPS
GRLLGKREAYAVNFGKVFEAAAENGKVMEINCQPSRLDLNDELIFRAKDYELKFCISTDSHSVSDLASMRYGLGQARRSW
LEKEDIVNTYPYSRLKEVFKKLRD

Sequences:

>Translated_584_residues
MRNREVAELLYETADIMEFQQIEWKPRAYRRAAQNIENFGEDIEKVYEKKGKKGLTEIPGVGESIADHIAEYLKTGKVEK
FEGLKGKAPSGTAELMEIRGLGAKKMKKLADKLEIKTMSDLKNAVKSHRIRRLSGFGVKSEENIARAIENYEKSHSRIPL
GKALPLAEEIISGLKEELKNGTPGLDLSRIIYTGSLRRLKETIGDIDILAEAEEADAGKIMDAFVSLPEAGQVVSKGRTR
SSVILKEGFAIDLRVVPPESYGAALQYFTGSKEHNIGLRNIALREGYKLSEYGLYSKNSGERIAGKSEEEIYRKLGLEYI
APELRENRGELKAAAKNELPELVDAGDLRGDLHMHTDYSEGAESLETMVEKAESMGYEYIAVTDHSRSQRIANGMDIETL
KEQWKEIKSLSKRFRIKVLRGSEVEILKDGSLDYPDEILKELDVVVGAVHSGFAASEREMTGRIITALENKHLDILAHPS
GRLLGKREAYAVNFGKVFEAAAENGKVMEINCQPSRLDLNDELIFRAKDYELKFCISTDSHSVSDLASMRYGLGQARRSW
LEKEDIVNTYPYSRLKEVFKKLRD
>Mature_584_residues
MRNREVAELLYETADIMEFQQIEWKPRAYRRAAQNIENFGEDIEKVYEKKGKKGLTEIPGVGESIADHIAEYLKTGKVEK
FEGLKGKAPSGTAELMEIRGLGAKKMKKLADKLEIKTMSDLKNAVKSHRIRRLSGFGVKSEENIARAIENYEKSHSRIPL
GKALPLAEEIISGLKEELKNGTPGLDLSRIIYTGSLRRLKETIGDIDILAEAEEADAGKIMDAFVSLPEAGQVVSKGRTR
SSVILKEGFAIDLRVVPPESYGAALQYFTGSKEHNIGLRNIALREGYKLSEYGLYSKNSGERIAGKSEEEIYRKLGLEYI
APELRENRGELKAAAKNELPELVDAGDLRGDLHMHTDYSEGAESLETMVEKAESMGYEYIAVTDHSRSQRIANGMDIETL
KEQWKEIKSLSKRFRIKVLRGSEVEILKDGSLDYPDEILKELDVVVGAVHSGFAASEREMTGRIITALENKHLDILAHPS
GRLLGKREAYAVNFGKVFEAAAENGKVMEINCQPSRLDLNDELIFRAKDYELKFCISTDSHSVSDLASMRYGLGQARRSW
LEKEDIVNTYPYSRLKEVFKKLRD

Specific function: Strictly DNA-template-directed DNA polymerase, preferentially acting on DNA structures containing gaps from one to a few nucleotides and bearing a phosphate group at the 5' end of the downstream DNA. The fact that PolX is able to conduct filling of a sing

COG id: COG1796

COG function: function code L; DNA polymerase IV (family X)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PHP family [H]

Homologues:

Organism=Homo sapiens, GI4505931, Length=352, Percent_Identity=26.7045454545455, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI7019491, Length=351, Percent_Identity=23.6467236467236, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI291463287, Length=351, Percent_Identity=23.6467236467236, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI291463291, Length=298, Percent_Identity=24.496644295302, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002054
- InterPro:   IPR010996
- InterPro:   IPR003583
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR016195
- InterPro:   IPR022311 [H]

Pfam domain/function: PF02811 PHP [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 65560; Mature: 65560

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNREVAELLYETADIMEFQQIEWKPRAYRRAAQNIENFGEDIEKVYEKKGKKGLTEIPG
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VGESIADHIAEYLKTGKVEKFEGLKGKAPSGTAELMEIRGLGAKKMKKLADKLEIKTMSD
CCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LKNAVKSHRIRRLSGFGVKSEENIARAIENYEKSHSRIPLGKALPLAEEIISGLKEELKN
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
GTPGLDLSRIIYTGSLRRLKETIGDIDILAEAEEADAGKIMDAFVSLPEAGQVVSKGRTR
CCCCCCHHHHHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHCCCCCHHHHHCCCCH
SSVILKEGFAIDLRVVPPESYGAALQYFTGSKEHNIGLRNIALREGYKLSEYGLYSKNSG
HHEEEECCCEEEEEEECCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCCHHHCCCCCCCCC
ERIAGKSEEEIYRKLGLEYIAPELRENRGELKAAAKNELPELVDAGDLRGDLHMHTDYSE
CCCCCCCHHHHHHHHCHHHHCHHHHCCHHHHHHHHHHCCHHHHCCCCCCCCEEECCCHHH
GAESLETMVEKAESMGYEYIAVTDHSRSQRIANGMDIETLKEQWKEIKSLSKRFRIKVLR
HHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEC
GSEVEILKDGSLDYPDEILKELDVVVGAVHSGFAASEREMTGRIITALENKHLDILAHPS
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCC
GRLLGKREAYAVNFGKVFEAAAENGKVMEINCQPSRLDLNDELIFRAKDYELKFCISTDS
CCCCCCCCCEEECHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEEECCC
HSVSDLASMRYGLGQARRSWLEKEDIVNTYPYSRLKEVFKKLRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRNREVAELLYETADIMEFQQIEWKPRAYRRAAQNIENFGEDIEKVYEKKGKKGLTEIPG
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VGESIADHIAEYLKTGKVEKFEGLKGKAPSGTAELMEIRGLGAKKMKKLADKLEIKTMSD
CCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LKNAVKSHRIRRLSGFGVKSEENIARAIENYEKSHSRIPLGKALPLAEEIISGLKEELKN
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
GTPGLDLSRIIYTGSLRRLKETIGDIDILAEAEEADAGKIMDAFVSLPEAGQVVSKGRTR
CCCCCCHHHHHHHHHHHHHHHHHCCHHEEECCCCCCHHHHHHHHHCCCCCHHHHHCCCCH
SSVILKEGFAIDLRVVPPESYGAALQYFTGSKEHNIGLRNIALREGYKLSEYGLYSKNSG
HHEEEECCCEEEEEEECCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCCHHHCCCCCCCCC
ERIAGKSEEEIYRKLGLEYIAPELRENRGELKAAAKNELPELVDAGDLRGDLHMHTDYSE
CCCCCCCHHHHHHHHCHHHHCHHHHCCHHHHHHHHHHCCHHHHCCCCCCCCEEECCCHHH
GAESLETMVEKAESMGYEYIAVTDHSRSQRIANGMDIETLKEQWKEIKSLSKRFRIKVLR
HHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEC
GSEVEILKDGSLDYPDEILKELDVVVGAVHSGFAASEREMTGRIITALENKHLDILAHPS
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEECCC
GRLLGKREAYAVNFGKVFEAAAENGKVMEINCQPSRLDLNDELIFRAKDYELKFCISTDS
CCCCCCCCCEEECHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEEECCC
HSVSDLASMRYGLGQARRSWLEKEDIVNTYPYSRLKEVFKKLRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]