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The map label for this gene is gltX

Identifier: 21227851

GI number: 21227851

Start: 2090005

End: 2091720

Strand: Direct

Name: gltX

Synonym: MM_1749

Alternate gene names: 21227851

Gene position: 2090005-2091720 (Clockwise)

Preceding gene: 21227850

Following gene: 21227853

Centisome position: 51.02

GC content: 48.31

Gene sequence:

>1716_bases
ATGACCTTAAGTCCTGAAGATATTAAAATAATTGAAAAATACGCTCTTCAAAATGCTGTAAAATACGGAAAAGCCCCACA
GCCCAAAGCTGTTATGGGTAAAGTTATGGGGGAATGTCCCCAGTTGAGGGCTGACCCCAGCGGGGTATCTGCTGCCCTTG
AAAATATAGTTTCAGAAATTGCTAAAGGAAACCCTGAAGCCTGGGAAGCCCGCCTGTCGGAAATCGCTCCCGAGCTTATA
GAGGCTCTGAGTGTAAAGAAAGAGCCTGACAAAGGCTTAAAACCTCTTGAGGGAGCCGAAACCGGTAAGGTTGTAATGCG
CTTTGCCCCAAATCCCAACGGACCCGCAACCCTGGGAAGTTCAAGGGGAATGGTTGTCAATTCCGAGTATGTAAAAGTGT
ATAAAGGAAAGTTTATCCTGCGCTTTGATGACACTGACCCTGATATAAAGCGTCCCATGCTCGAAGCCTATGACTGGTAC
ATGGACGACTTTAAATGGCTCGGGGTTGTGCCTGACCAGGTTGTACGTGCCTCTGACCGTTTCCCGATTTATTACGATTA
TGCAAGAAAACTGATTGAGATGGGAAAAGCCTATGTCTGTTTCTGTAAAGGAGAAGACTTCAAAAGGTTAAAAGACTCCA
AAAAGGCCTGCCCTCACAGGGACACAGACCCTGAAGAAAACCTTATGCACTGGGAAAAGATGCTTGCCGGAGAATATGAA
GACCAGCAGGCTGTTCTGCGCATAAAGACCGATATAGAGCACAAAGACCCTGCACTTCGGGACTGGGGAGCATTCAGGAT
TAGAAAAATGTCCCACCCACGCCCTGAGATTGGAAATAAATACATCGTCTGGCCCCTTCTGGACTTTGCAGGTGCTATCG
AAGACCACGAGCTCGGTATGACCCACATCATCCGGGGTAAAGACCTTATCGACAGTGAAAAACGGCAGACATACATTTAC
AAATACTTCGGCTGGACATACCCGAAAACAACTCACTGGGGCAGGGTAAAGATCCATGAGTTCGGAAAGTTCAGCACAAG
TACCCTGCGTAAAGCTATCGAATCCGGAGAATACAGCGGCTGGGACGACCCCCGCCTGCCGACAATAAGGGCAATCAGGC
GCCGTGGAATTAGGGCTGAAGCTCTCAAAAAGTTCATGATAGAAATGGGAGTCGGGATGACCGATGTGAGCATCAGCATG
GAATCTCTTTATGCCGAGAACCGCAAAATCGTGGACCCGGTTGCAAACCGCTATTTCTTTGTCTGGAACCCTGTGGAGCT
TGAAATTGAAGGTATGAAACCTGTGGTCGCAAAGGTCCCCCGTCACCCAACAGACCATGCAAGAGGAATGAGGGAAATCT
CTATCGAAAATAAGGTTCTTGTCTGTGCCGAGGACATTGAAAAGCTCAATGTAGGTTCTGTCCTCCGCTTAAAAGATCTC
TGCAACGTTGAAATTACTTCCCTTTCCCCGCTGCGGGTTAAGCGCTCCGAAACCTCTCTCGAAGACCTCAAAAAAGCAAA
AGGAAAAATCATCCACTGGGTGCCGGTTGATGGAATTCCTGTGAAAGTCTGCGGGCCAGAGGGCGATATCGAAGGGACAG
GAGAACGCGGGATCGAGACCGAGCTTGATAATATTGTCCAGTTTGAACGCTTCGGTTTCTGCAGGATCGACGCCGTAGAC
GGAGAAAATGTCGTGGCGTACTTTGCTCACAAATGA

Upstream 100 bases:

>100_bases
CTGTCCACCTCTGTGCGGATTATCCATTCAGTTAAAATTTATGATATTCTTATCGCCTCCGATTAAGATTTAATCAAGGT
TTATTTCTGGAGAATTTTCT

Downstream 100 bases:

>100_bases
TCAGGGAAAGAAAACGGACAGAAAACTGCCCGTTCTTTTCTTTCTATCTCTTTTTTCCTGTCTTTTATTTCAGTTACTTT
CTCCTCTTCAGGAAATAAGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MTLSPEDIKIIEKYALQNAVKYGKAPQPKAVMGKVMGECPQLRADPSGVSAALENIVSEIAKGNPEAWEARLSEIAPELI
EALSVKKEPDKGLKPLEGAETGKVVMRFAPNPNGPATLGSSRGMVVNSEYVKVYKGKFILRFDDTDPDIKRPMLEAYDWY
MDDFKWLGVVPDQVVRASDRFPIYYDYARKLIEMGKAYVCFCKGEDFKRLKDSKKACPHRDTDPEENLMHWEKMLAGEYE
DQQAVLRIKTDIEHKDPALRDWGAFRIRKMSHPRPEIGNKYIVWPLLDFAGAIEDHELGMTHIIRGKDLIDSEKRQTYIY
KYFGWTYPKTTHWGRVKIHEFGKFSTSTLRKAIESGEYSGWDDPRLPTIRAIRRRGIRAEALKKFMIEMGVGMTDVSISM
ESLYAENRKIVDPVANRYFFVWNPVELEIEGMKPVVAKVPRHPTDHARGMREISIENKVLVCAEDIEKLNVGSVLRLKDL
CNVEITSLSPLRVKRSETSLEDLKKAKGKIIHWVPVDGIPVKVCGPEGDIEGTGERGIETELDNIVQFERFGFCRIDAVD
GENVVAYFAHK

Sequences:

>Translated_571_residues
MTLSPEDIKIIEKYALQNAVKYGKAPQPKAVMGKVMGECPQLRADPSGVSAALENIVSEIAKGNPEAWEARLSEIAPELI
EALSVKKEPDKGLKPLEGAETGKVVMRFAPNPNGPATLGSSRGMVVNSEYVKVYKGKFILRFDDTDPDIKRPMLEAYDWY
MDDFKWLGVVPDQVVRASDRFPIYYDYARKLIEMGKAYVCFCKGEDFKRLKDSKKACPHRDTDPEENLMHWEKMLAGEYE
DQQAVLRIKTDIEHKDPALRDWGAFRIRKMSHPRPEIGNKYIVWPLLDFAGAIEDHELGMTHIIRGKDLIDSEKRQTYIY
KYFGWTYPKTTHWGRVKIHEFGKFSTSTLRKAIESGEYSGWDDPRLPTIRAIRRRGIRAEALKKFMIEMGVGMTDVSISM
ESLYAENRKIVDPVANRYFFVWNPVELEIEGMKPVVAKVPRHPTDHARGMREISIENKVLVCAEDIEKLNVGSVLRLKDL
CNVEITSLSPLRVKRSETSLEDLKKAKGKIIHWVPVDGIPVKVCGPEGDIEGTGERGIETELDNIVQFERFGFCRIDAVD
GENVVAYFAHK
>Mature_570_residues
TLSPEDIKIIEKYALQNAVKYGKAPQPKAVMGKVMGECPQLRADPSGVSAALENIVSEIAKGNPEAWEARLSEIAPELIE
ALSVKKEPDKGLKPLEGAETGKVVMRFAPNPNGPATLGSSRGMVVNSEYVKVYKGKFILRFDDTDPDIKRPMLEAYDWYM
DDFKWLGVVPDQVVRASDRFPIYYDYARKLIEMGKAYVCFCKGEDFKRLKDSKKACPHRDTDPEENLMHWEKMLAGEYED
QQAVLRIKTDIEHKDPALRDWGAFRIRKMSHPRPEIGNKYIVWPLLDFAGAIEDHELGMTHIIRGKDLIDSEKRQTYIYK
YFGWTYPKTTHWGRVKIHEFGKFSTSTLRKAIESGEYSGWDDPRLPTIRAIRRRGIRAEALKKFMIEMGVGMTDVSISME
SLYAENRKIVDPVANRYFFVWNPVELEIEGMKPVVAKVPRHPTDHARGMREISIENKVLVCAEDIEKLNVGSVLRLKDLC
NVEITSLSPLRVKRSETSLEDLKKAKGKIIHWVPVDGIPVKVCGPEGDIEGTGERGIETELDNIVQFERFGFCRIDAVDG
ENVVAYFAHK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI62241042, Length=418, Percent_Identity=31.8181818181818, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI4826960, Length=372, Percent_Identity=29.0322580645161, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI134288884, Length=250, Percent_Identity=29.2, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1786895, Length=457, Percent_Identity=29.1028446389497, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1788743, Length=312, Percent_Identity=25.6410256410256, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17511007, Length=500, Percent_Identity=28.2, Blast_Score=200, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17543332, Length=448, Percent_Identity=30.1339285714286, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71993394, Length=254, Percent_Identity=27.9527559055118, Blast_Score=94, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321192, Length=507, Percent_Identity=28.7968441814596, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6324742, Length=389, Percent_Identity=31.3624678663239, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6324540, Length=242, Percent_Identity=28.099173553719, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24649466, Length=398, Percent_Identity=30.6532663316583, Blast_Score=195, Evalue=8e-50,
Organism=Drosophila melanogaster, GI17864488, Length=365, Percent_Identity=29.5890410958904, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24665321, Length=332, Percent_Identity=25, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_METMA (Q8PW52)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633773.1
- ProteinModelPortal:   Q8PW52
- SMR:   Q8PW52
- GeneID:   1480091
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1749
- NMPDR:   fig|192952.1.peg.1749
- HOGENOM:   HBG334108
- OMA:   MRFAPNP
- ProtClustDB:   PRK04156
- BioCyc:   MMAZ192952:MM1749-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_A
- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00463

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.17

Molecular weight: Translated: 64986; Mature: 64854

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSPEDIKIIEKYALQNAVKYGKAPQPKAVMGKVMGECPQLRADPSGVSAALENIVSEI
CCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AKGNPEAWEARLSEIAPELIEALSVKKEPDKGLKPLEGAETGKVVMRFAPNPNGPATLGS
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
SRGMVVNSEYVKVYKGKFILRFDDTDPDIKRPMLEAYDWYMDDFKWLGVVPDQVVRASDR
CCCEEEECCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHCCCC
FPIYYDYARKLIEMGKAYVCFCKGEDFKRLKDSKKACPHRDTDPEENLMHWEKMLAGEYE
CCEEHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCC
DQQAVLRIKTDIEHKDPALRDWGAFRIRKMSHPRPEIGNKYIVWPLLDFAGAIEDHELGM
CCCEEEEEECCCCCCCCCCCCCCHHHEECCCCCCCCCCCEEEEEEHHHHHCCCCCCCCCC
THIIRGKDLIDSEKRQTYIYKYFGWTYPKTTHWGRVKIHEFGKFSTSTLRKAIESGEYSG
CEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCC
WDDPRLPTIRAIRRRGIRAEALKKFMIEMGVGMTDVSISMESLYAENRKIVDPVANRYFF
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHCHHHCCEEE
VWNPVELEIEGMKPVVAKVPRHPTDHARGMREISIENKVLVCAEDIEKLNVGSVLRLKDL
EECCEEEEECCCCHHHHHCCCCCCHHHCCCEEEECCCCEEEEEHHHHHCCCCCEEEHHHH
CNVEITSLSPLRVKRSETSLEDLKKAKGKIIHWVPVDGIPVKVCGPEGDIEGTGERGIET
CCEEEECCCCEEEECCCCHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCHH
ELDNIVQFERFGFCRIDAVDGENVVAYFAHK
HHHHHHHHHHCCCEEEEECCCCCEEEEEECC
>Mature Secondary Structure 
TLSPEDIKIIEKYALQNAVKYGKAPQPKAVMGKVMGECPQLRADPSGVSAALENIVSEI
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AKGNPEAWEARLSEIAPELIEALSVKKEPDKGLKPLEGAETGKVVMRFAPNPNGPATLGS
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
SRGMVVNSEYVKVYKGKFILRFDDTDPDIKRPMLEAYDWYMDDFKWLGVVPDQVVRASDR
CCCEEEECCEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHCCCC
FPIYYDYARKLIEMGKAYVCFCKGEDFKRLKDSKKACPHRDTDPEENLMHWEKMLAGEYE
CCEEHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCC
DQQAVLRIKTDIEHKDPALRDWGAFRIRKMSHPRPEIGNKYIVWPLLDFAGAIEDHELGM
CCCEEEEEECCCCCCCCCCCCCCHHHEECCCCCCCCCCCEEEEEEHHHHHCCCCCCCCCC
THIIRGKDLIDSEKRQTYIYKYFGWTYPKTTHWGRVKIHEFGKFSTSTLRKAIESGEYSG
CEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCC
WDDPRLPTIRAIRRRGIRAEALKKFMIEMGVGMTDVSISMESLYAENRKIVDPVANRYFF
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHCHHHCCEEE
VWNPVELEIEGMKPVVAKVPRHPTDHARGMREISIENKVLVCAEDIEKLNVGSVLRLKDL
EECCEEEEECCCCHHHHHCCCCCCHHHCCCEEEECCCCEEEEEHHHHHCCCCCEEEHHHH
CNVEITSLSPLRVKRSETSLEDLKKAKGKIIHWVPVDGIPVKVCGPEGDIEGTGERGIET
CCEEEECCCCEEEECCCCHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCHH
ELDNIVQFERFGFCRIDAVDGENVVAYFAHK
HHHHHHHHHHCCCEEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824