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The map label for this gene is hemL [H]

Identifier: 21227845

GI number: 21227845

Start: 2084644

End: 2085918

Strand: Direct

Name: hemL [H]

Synonym: MM_1743

Alternate gene names: 21227845

Gene position: 2084644-2085918 (Clockwise)

Preceding gene: 21227844

Following gene: 21227846

Centisome position: 50.89

GC content: 49.1

Gene sequence:

>1275_bases
ATGGTATCTGAGATTACACTTGAGAAGTCCAGACAGATGTATGAGAAAGCAAAAACCCTTATTCCGGGCGGGGTTAGCAG
CCCGGTAAGAGCTATCAAGCCCTATCCTTTCTATACCGCTTCTGCTAATGGTTCGAAGATAAGGGACCTTGACGGAAACG
AATATATTGACTACTGCCTTGCCTACGGACCTGCTATCCTCGGGCACAACCATCCTGTCATAAAAGAAGCAATCAGGCAG
CAGCTTGACAGGGGATGGCTTTACGGGACCCCTACCGAGCTTGAAGTAACCCTTGCAGAAAAGGTTGCAAGCTACTATCC
AAGCATTGACATGCTCCGTTTTGTGTCTACAGGTACGGAAGCGACTATGAGTGCCCTCCGCCTCGCACGCGGCTTTACGC
GCAGGAACAAATTCATCAAGATTGAAGGCGGGTTTCACGGAGCTCATGACGCCGTGCTCGTAAAAGCAGGTTCAGGGGCA
ACAACCCTTGGAGAGCCTGATTCTCTGGGCATCCCTGCAGATTTCACGAAACACACCCTGCAGGCTCCTTATAATGATAT
TGAGACGATGACCTCGCTTGTGGAAAAGAACAGGGACGACCTTGCAGCAGTGATCATAGAACCTGTACTCGGAAATATAG
GCCCTGTGCTGCCACTTCCGGGATACCTCGAAGAGCTCAGGAAACTCACGGAAGAAAACGATGTACTTCTCATTTTCGAT
GAAGTTATTACCGGTTTCAGGCTTGCTATGGGCGGGGCACAGGAATATTTCGGAGTCGTGCCTGACATGACCACTCTTGG
AAAGATTGTGGGCGGAGGTCTTCCAATCGGTGTCTTCGGAGGAAAGCGTGAAATTATGGAAATGATTGCACCTTCCGGAG
CTGTTTATCAGGCAGGTACATTCAGCGGTAGCCCATGTTCAGTGGCTGCTGGCATTGCCATGGTCGATTACCTGAATGAG
GAAAATATTCATGCAAAGCTAAATTCAGCCGGCGATTATATGAGGGCTGCACTTTCGGAAATCGTTGAGGACGAAGGGCT
TGACTACCACGTATGCGGAATAGCTTCCATGTTCAAGATCTTCTTCGGGGACGAGCCGCATAATTACCAGGAAGTCCTGA
AGTGTGATAAAGAGGGTTACCTTGCTTTCTTCCACAGGATGCTTGCAAGCGGGGTTTTCCTGCCTCCTTCACAGTTTGAG
ACCAACTTCATATCAGCAGTCCACAGTGACGAGGATATTGAAAAGACCCTTGAAGCTTATTCGGAAAATCTCTGA

Upstream 100 bases:

>100_bases
AATTTAAATTTGATTTACTTACTCAATTTAAATTTGATTTACTTACTAAATTAATCACTTACCCCTTTATCAAACGTTCA
AATCTATCCGGTGAGAGTTC

Downstream 100 bases:

>100_bases
GGGCTTACGTTCCTGAGGATTTGTCTTTCAGATTTATGTTTTAAAACGTTTGAGGATTCCACATGATAATAGGTACCCGG
GGCAGCCAGCTTGCGCTTGC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MVSEITLEKSRQMYEKAKTLIPGGVSSPVRAIKPYPFYTASANGSKIRDLDGNEYIDYCLAYGPAILGHNHPVIKEAIRQ
QLDRGWLYGTPTELEVTLAEKVASYYPSIDMLRFVSTGTEATMSALRLARGFTRRNKFIKIEGGFHGAHDAVLVKAGSGA
TTLGEPDSLGIPADFTKHTLQAPYNDIETMTSLVEKNRDDLAAVIIEPVLGNIGPVLPLPGYLEELRKLTEENDVLLIFD
EVITGFRLAMGGAQEYFGVVPDMTTLGKIVGGGLPIGVFGGKREIMEMIAPSGAVYQAGTFSGSPCSVAAGIAMVDYLNE
ENIHAKLNSAGDYMRAALSEIVEDEGLDYHVCGIASMFKIFFGDEPHNYQEVLKCDKEGYLAFFHRMLASGVFLPPSQFE
TNFISAVHSDEDIEKTLEAYSENL

Sequences:

>Translated_424_residues
MVSEITLEKSRQMYEKAKTLIPGGVSSPVRAIKPYPFYTASANGSKIRDLDGNEYIDYCLAYGPAILGHNHPVIKEAIRQ
QLDRGWLYGTPTELEVTLAEKVASYYPSIDMLRFVSTGTEATMSALRLARGFTRRNKFIKIEGGFHGAHDAVLVKAGSGA
TTLGEPDSLGIPADFTKHTLQAPYNDIETMTSLVEKNRDDLAAVIIEPVLGNIGPVLPLPGYLEELRKLTEENDVLLIFD
EVITGFRLAMGGAQEYFGVVPDMTTLGKIVGGGLPIGVFGGKREIMEMIAPSGAVYQAGTFSGSPCSVAAGIAMVDYLNE
ENIHAKLNSAGDYMRAALSEIVEDEGLDYHVCGIASMFKIFFGDEPHNYQEVLKCDKEGYLAFFHRMLASGVFLPPSQFE
TNFISAVHSDEDIEKTLEAYSENL
>Mature_424_residues
MVSEITLEKSRQMYEKAKTLIPGGVSSPVRAIKPYPFYTASANGSKIRDLDGNEYIDYCLAYGPAILGHNHPVIKEAIRQ
QLDRGWLYGTPTELEVTLAEKVASYYPSIDMLRFVSTGTEATMSALRLARGFTRRNKFIKIEGGFHGAHDAVLVKAGSGA
TTLGEPDSLGIPADFTKHTLQAPYNDIETMTSLVEKNRDDLAAVIIEPVLGNIGPVLPLPGYLEELRKLTEENDVLLIFD
EVITGFRLAMGGAQEYFGVVPDMTTLGKIVGGGLPIGVFGGKREIMEMIAPSGAVYQAGTFSGSPCSVAAGIAMVDYLNE
ENIHAKLNSAGDYMRAALSEIVEDEGLDYHVCGIASMFKIFFGDEPHNYQEVLKCDKEGYLAFFHRMLASGVFLPPSQFE
TNFISAVHSDEDIEKTLEAYSENL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=327, Percent_Identity=29.0519877675841, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI24119277, Length=347, Percent_Identity=24.4956772334294, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI13994255, Length=332, Percent_Identity=25.6024096385542, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI37574042, Length=365, Percent_Identity=26.5753424657534, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI226442709, Length=327, Percent_Identity=26.2996941896024, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI226442705, Length=364, Percent_Identity=25.5494505494505, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI284507298, Length=278, Percent_Identity=28.4172661870504, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1786349, Length=418, Percent_Identity=49.2822966507177, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1789016, Length=384, Percent_Identity=28.90625, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI145693181, Length=307, Percent_Identity=33.5504885993485, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1788044, Length=338, Percent_Identity=30.4733727810651, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787560, Length=348, Percent_Identity=31.6091954022989, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789759, Length=328, Percent_Identity=29.5731707317073, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1786991, Length=300, Percent_Identity=27.6666666666667, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71992977, Length=334, Percent_Identity=27.2455089820359, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI25144271, Length=366, Percent_Identity=27.3224043715847, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32564660, Length=338, Percent_Identity=28.4023668639053, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=220, Percent_Identity=28.1818181818182, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=331, Percent_Identity=26.8882175226586, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324432, Length=323, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357415, Length=317, Percent_Identity=27.7602523659306, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI161085790, Length=349, Percent_Identity=26.0744985673352, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI28574759, Length=349, Percent_Identity=26.0744985673352, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46306; Mature: 46306

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSEITLEKSRQMYEKAKTLIPGGVSSPVRAIKPYPFYTASANGSKIRDLDGNEYIDYCL
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEECCCCCEEECCCCCHHHHHHH
AYGPAILGHNHPVIKEAIRQQLDRGWLYGTPTELEVTLAEKVASYYPSIDMLRFVSTGTE
HHCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHCCHH
ATMSALRLARGFTRRNKFIKIEGGFHGAHDAVLVKAGSGATTLGEPDSLGIPADFTKHTL
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
QAPYNDIETMTSLVEKNRDDLAAVIIEPVLGNIGPVLPLPGYLEELRKLTEENDVLLIFD
HCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEHH
EVITGFRLAMGGAQEYFGVVPDMTTLGKIVGGGLPIGVFGGKREIMEMIAPSGAVYQAGT
HHHHHHHHHHCCHHHHHCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCEEECCC
FSGSPCSVAAGIAMVDYLNEENIHAKLNSAGDYMRAALSEIVEDEGLDYHVCGIASMFKI
CCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHH
FFGDEPHNYQEVLKCDKEGYLAFFHRMLASGVFLPPSQFETNFISAVHSDEDIEKTLEAY
HCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
SENL
HCCC
>Mature Secondary Structure
MVSEITLEKSRQMYEKAKTLIPGGVSSPVRAIKPYPFYTASANGSKIRDLDGNEYIDYCL
CCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEECCCCCEEECCCCCHHHHHHH
AYGPAILGHNHPVIKEAIRQQLDRGWLYGTPTELEVTLAEKVASYYPSIDMLRFVSTGTE
HHCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHCCHH
ATMSALRLARGFTRRNKFIKIEGGFHGAHDAVLVKAGSGATTLGEPDSLGIPADFTKHTL
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
QAPYNDIETMTSLVEKNRDDLAAVIIEPVLGNIGPVLPLPGYLEELRKLTEENDVLLIFD
HCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEHH
EVITGFRLAMGGAQEYFGVVPDMTTLGKIVGGGLPIGVFGGKREIMEMIAPSGAVYQAGT
HHHHHHHHHHCCHHHHHCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCEEECCC
FSGSPCSVAAGIAMVDYLNEENIHAKLNSAGDYMRAALSEIVEDEGLDYHVCGIASMFKI
CCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHH
FFGDEPHNYQEVLKCDKEGYLAFFHRMLASGVFLPPSQFETNFISAVHSDEDIEKTLEAY
HCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
SENL
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]