Click here to switch to the map view.

The map label for this gene is hemC

Identifier: 21227846

GI number: 21227846

Start: 2085981

End: 2086931

Strand: Direct

Name: hemC

Synonym: MM_1744

Alternate gene names: 21227846

Gene position: 2085981-2086931 (Clockwise)

Preceding gene: 21227845

Following gene: 161485673

Centisome position: 50.92

GC content: 51.95

Gene sequence:

>951_bases
ATGATAATAGGTACCCGGGGCAGCCAGCTTGCGCTTGCCCAGACCGAGAATGTTGCACGCCTCCTTAAAGAAAAAGGCGT
TGAAACCAGTATTAAGATTATAAAAACCAGCGGTGACCGGTTTACTGACCGCCCTCTGCACGCCGTATCCGGAGGAGTCG
GGGCTTTTGTCCGGGAACTGGATGATGTCATGCTTGCAGGGGAAATAGATATCGCAGTCCATTCCATGAAGGACATGCCC
ACAATCCGTCCTAAAGCGATTCCCACCGTTGCTGTCCTGAAGAGGGACACACCTTTTGATATTCTGCTCACATACGACGG
GACTCCGCTTGACGAGCTTCCTGAACAGTCAATTATAGGCACAAGTTCCCTGAGAAGGACTGCCCAGATCAGGAGGTACA
GGCCTGACATTATTACCCAGAACCTCAGAGGCAATATCGACACAAGGCTCAGAAAACTCAGGGAAGGTCAGTATGACGGG
ATTATGCTCGCGAAGGCAGGTCTTGAACGCATGGGCTGGGAGATTGAAGGGGAAATCTTTTCCCCTGACTTTTTCTGCCC
TTCTCCTAACCAGGGCACGGTTGCAGTGGTAACAAGAGAAGGCACTGAAGCTGAAGCCGCAGTTTCCATGCTTGACCATA
CCGAGAGCAGGATTGTTACCGAAATCGAACGCATCCTTATCGCCGAGCTCGGAGGGGGATGTACAACTCCTGTGGGTTCG
TATGCTGAAATTACACCTGACAGGCAGGAGATCCATGTCCGGGCTGAAGTCCTTTCTCTTGACGGCAGGGAAGCTATCAG
GATCGACGAATTTATCCCCCTGCGCGGAGGAATTGAGAAAGCCAGGGAACTCGGGCACAGACTCGTGGAGATGGGCGGAA
GGAGACTTGCTGAGGAAGCGCTCCTTCAGATTTCCAGAGGTGCCGATTCCGAAAATTCCTGTGATTATTGA

Upstream 100 bases:

>100_bases
TTGAAAAGACCCTTGAAGCTTATTCGGAAAATCTCTGAGGGCTTACGTTCCTGAGGATTTGTCTTTCAGATTTATGTTTT
AAAACGTTTGAGGATTCCAC

Downstream 100 bases:

>100_bases
TGCAGAAACTGCCTGCGGGCAGGAAACGGAGTTATTTAATGTATACTCTTACCGGACTTAAATTAAAAAATCCCACTATT
CTTGCAGCCGGGGTGCTCGG

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MIIGTRGSQLALAQTENVARLLKEKGVETSIKIIKTSGDRFTDRPLHAVSGGVGAFVRELDDVMLAGEIDIAVHSMKDMP
TIRPKAIPTVAVLKRDTPFDILLTYDGTPLDELPEQSIIGTSSLRRTAQIRRYRPDIITQNLRGNIDTRLRKLREGQYDG
IMLAKAGLERMGWEIEGEIFSPDFFCPSPNQGTVAVVTREGTEAEAAVSMLDHTESRIVTEIERILIAELGGGCTTPVGS
YAEITPDRQEIHVRAEVLSLDGREAIRIDEFIPLRGGIEKARELGHRLVEMGGRRLAEEALLQISRGADSENSCDY

Sequences:

>Translated_316_residues
MIIGTRGSQLALAQTENVARLLKEKGVETSIKIIKTSGDRFTDRPLHAVSGGVGAFVRELDDVMLAGEIDIAVHSMKDMP
TIRPKAIPTVAVLKRDTPFDILLTYDGTPLDELPEQSIIGTSSLRRTAQIRRYRPDIITQNLRGNIDTRLRKLREGQYDG
IMLAKAGLERMGWEIEGEIFSPDFFCPSPNQGTVAVVTREGTEAEAAVSMLDHTESRIVTEIERILIAELGGGCTTPVGS
YAEITPDRQEIHVRAEVLSLDGREAIRIDEFIPLRGGIEKARELGHRLVEMGGRRLAEEALLQISRGADSENSCDY
>Mature_316_residues
MIIGTRGSQLALAQTENVARLLKEKGVETSIKIIKTSGDRFTDRPLHAVSGGVGAFVRELDDVMLAGEIDIAVHSMKDMP
TIRPKAIPTVAVLKRDTPFDILLTYDGTPLDELPEQSIIGTSSLRRTAQIRRYRPDIITQNLRGNIDTRLRKLREGQYDG
IMLAKAGLERMGWEIEGEIFSPDFFCPSPNQGTVAVVTREGTEAEAAVSMLDHTESRIVTEIERILIAELGGGCTTPVGS
YAEITPDRQEIHVRAEVLSLDGREAIRIDEFIPLRGGIEKARELGHRLVEMGGRRLAEEALLQISRGADSENSCDY

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=273, Percent_Identity=36.6300366300366, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI66933009, Length=273, Percent_Identity=36.6300366300366, Blast_Score=163, Evalue=2e-40,
Organism=Escherichia coli, GI48994974, Length=309, Percent_Identity=38.8349514563107, Blast_Score=179, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6319996, Length=318, Percent_Identity=33.0188679245283, Blast_Score=149, Evalue=5e-37,
Organism=Drosophila melanogaster, GI20130425, Length=333, Percent_Identity=35.1351351351351, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_METMA (Q8PW57)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633768.1
- ProteinModelPortal:   Q8PW57
- SMR:   Q8PW57
- GeneID:   1480086
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1744
- NMPDR:   fig|192952.1.peg.1744
- HOGENOM:   HBG624842
- OMA:   RATEVFD
- ProtClustDB:   PRK00072
- BioCyc:   MMAZ192952:MM1744-MONOMER
- BRENDA:   2.5.1.61
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34847; Mature: 34847

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGTRGSQLALAQTENVARLLKEKGVETSIKIIKTSGDRFTDRPLHAVSGGVGAFVREL
CEECCCCCEEEEHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHCCCHHHHHHHH
DDVMLAGEIDIAVHSMKDMPTIRPKAIPTVAVLKRDTPFDILLTYDGTPLDELPEQSIIG
HHHHCCCCCEEEEECHHCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHHCCCHHHCC
TSSLRRTAQIRRYRPDIITQNLRGNIDTRLRKLREGQYDGIMLAKAGLERMGWEIEGEIF
HHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHCCEEECEEE
SPDFFCPSPNQGTVAVVTREGTEAEAAVSMLDHTESRIVTEIERILIAELGGGCTTPVGS
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YAEITPDRQEIHVRAEVLSLDGREAIRIDEFIPLRGGIEKARELGHRLVEMGGRRLAEEA
CCCCCCCHHHEEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
LLQISRGADSENSCDY
HHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIIGTRGSQLALAQTENVARLLKEKGVETSIKIIKTSGDRFTDRPLHAVSGGVGAFVREL
CEECCCCCEEEEHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHCCCHHHHHHHH
DDVMLAGEIDIAVHSMKDMPTIRPKAIPTVAVLKRDTPFDILLTYDGTPLDELPEQSIIG
HHHHCCCCCEEEEECHHCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHHCCCHHHCC
TSSLRRTAQIRRYRPDIITQNLRGNIDTRLRKLREGQYDGIMLAKAGLERMGWEIEGEIF
HHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHCCEEECEEE
SPDFFCPSPNQGTVAVVTREGTEAEAAVSMLDHTESRIVTEIERILIAELGGGCTTPVGS
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YAEITPDRQEIHVRAEVLSLDGREAIRIDEFIPLRGGIEKARELGHRLVEMGGRRLAEEA
CCCCCCCHHHEEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
LLQISRGADSENSCDY
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824