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The map label for this gene is nirJ [H]

Identifier: 21227839

GI number: 21227839

Start: 2078638

End: 2079687

Strand: Direct

Name: nirJ [H]

Synonym: MM_1737

Alternate gene names: 21227839

Gene position: 2078638-2079687 (Clockwise)

Preceding gene: 21227838

Following gene: 21227840

Centisome position: 50.74

GC content: 51.62

Gene sequence:

>1050_bases
ATGATTGCCATGACAAACCCACCACGGCTTATTGCATGGGAACTGACTGCAGGCTGCAACCTGAACTGCGTGCACTGCAG
GGGAGCTTCCACCTCTTCAGTTCCCGAAGGCGAGCTTACAACCGAAGAAGCGAAACACTTCATTGATGATGTTGTGGAGC
TTGGAAAACCAATCCTCATCCTGAGCGGAGGCGAGCCTCTTACCAGAACGGACGTCTTTGAAATAGCCCGCTACGGCACT
GATGCCGGGCTCCGTGTAGTGCTCGCAACAAACGGGACCCTTCTGACCCCTGAGATCGTGAAAAAATTAAGAGATGCCGG
AGTGCAGAGGCTGAGCATCAGCCTTGACGGAGCGACTGCAAAGACTCATGACGAGTTCCGCGGCGTGCCCGGAGCCTTTG
AAAGGACGCTTGCAGGCATTGAAATTCTCAGGAAAGCAGATTTCCCCTTCCAGATCAACACCACGATTTCAAGGCGCAAC
CTTGAAGAAATCCCGAAGACCTTTGAGCTTGCAAAAGAGCTCGGGGCAGTTGCTTACCATGTCTTTTTCCTTGTGCCCAC
GGGAAGGGGAGAAGAGTCCGATGAAGTTTCCCCTGCAGACTATGAGCGCGTCCTTCACTGGTTCTATGACATGCAGAAAG
AATCGGAAATCCAGCTGAAAGCGACCTGTGCTCCTCATTATTTCAGGATAATGCGCCAGAGGGCAAAAAAAGAAGGCATT
GAAATTTCCGTAAAGACCCACGGCTATGAAGCCATGACAAAGGGCTGCCTCGGAGGGACTGGTTTCTGTTTCGTGTCAAG
TGTTGGTGAGGTCTATCCCTGCGGCTACCTTCCGGTGCTTGCCGGGGATATAAGAGCACAACCTTTCAAAGATGTCTGGG
AGAACTCCGAGGTCTTCAGGAAATTGAGGGATCCCGAAGAGCTTAAAGGAAAGTGCGGGATATGTGAATATAAAAAAGTC
TGTGCAGGCTGCCGGGCAAGAGCCTATGCTGCCACGGGTGACTATCTCGAAGAAGAGCCTTACTGCATATACAGGCCCGG
AAAAAAGTGA

Upstream 100 bases:

>100_bases
TTCTGACCTATATTCCATCCTTTGATTATATGCAAATCCCTAATTATGGTTAATTTGTAAATCTATTAACCTTGACTTCC
ATAATATAAACCATTAATTC

Downstream 100 bases:

>100_bases
TAGTGATCGAGATGATTGACATTGATAGTTTAAAAGATCAGCTCCGAAAAGAGGCTGCCGAGTTTTCCTGTGATATGGAT
CCTAATGACAGGGAAGTTCC

Product: Heme biosynthesis protein

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MIAMTNPPRLIAWELTAGCNLNCVHCRGASTSSVPEGELTTEEAKHFIDDVVELGKPILILSGGEPLTRTDVFEIARYGT
DAGLRVVLATNGTLLTPEIVKKLRDAGVQRLSISLDGATAKTHDEFRGVPGAFERTLAGIEILRKADFPFQINTTISRRN
LEEIPKTFELAKELGAVAYHVFFLVPTGRGEESDEVSPADYERVLHWFYDMQKESEIQLKATCAPHYFRIMRQRAKKEGI
EISVKTHGYEAMTKGCLGGTGFCFVSSVGEVYPCGYLPVLAGDIRAQPFKDVWENSEVFRKLRDPEELKGKCGICEYKKV
CAGCRARAYAATGDYLEEEPYCIYRPGKK

Sequences:

>Translated_349_residues
MIAMTNPPRLIAWELTAGCNLNCVHCRGASTSSVPEGELTTEEAKHFIDDVVELGKPILILSGGEPLTRTDVFEIARYGT
DAGLRVVLATNGTLLTPEIVKKLRDAGVQRLSISLDGATAKTHDEFRGVPGAFERTLAGIEILRKADFPFQINTTISRRN
LEEIPKTFELAKELGAVAYHVFFLVPTGRGEESDEVSPADYERVLHWFYDMQKESEIQLKATCAPHYFRIMRQRAKKEGI
EISVKTHGYEAMTKGCLGGTGFCFVSSVGEVYPCGYLPVLAGDIRAQPFKDVWENSEVFRKLRDPEELKGKCGICEYKKV
CAGCRARAYAATGDYLEEEPYCIYRPGKK
>Mature_349_residues
MIAMTNPPRLIAWELTAGCNLNCVHCRGASTSSVPEGELTTEEAKHFIDDVVELGKPILILSGGEPLTRTDVFEIARYGT
DAGLRVVLATNGTLLTPEIVKKLRDAGVQRLSISLDGATAKTHDEFRGVPGAFERTLAGIEILRKADFPFQINTTISRRN
LEEIPKTFELAKELGAVAYHVFFLVPTGRGEESDEVSPADYERVLHWFYDMQKESEIQLKATCAPHYFRIMRQRAKKEGI
EISVKTHGYEAMTKGCLGGTGFCFVSSVGEVYPCGYLPVLAGDIRAQPFKDVWENSEVFRKLRDPEELKGKCGICEYKKV
CAGCRARAYAATGDYLEEEPYCIYRPGKK

Specific function: Together With Moac, Is Involved In The Conversion Of A Guanosine Derivative (Gxp) Into Molybdopterin Precursor Z. [C]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

Organism=Escherichia coli, GI1786999, Length=228, Percent_Identity=27.6315789473684, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 38803; Mature: 38803

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAMTNPPRLIAWELTAGCNLNCVHCRGASTSSVPEGELTTEEAKHFIDDVVELGKPILI
CCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
LSGGEPLTRTDVFEIARYGTDAGLRVVLATNGTLLTPEIVKKLRDAGVQRLSISLDGATA
EECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCEECHHHHHHHHHCCCEEEEEEECCCCC
KTHDEFRGVPGAFERTLAGIEILRKADFPFQINTTISRRNLEEIPKTFELAKELGAVAYH
CCCHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHCCHHHHHHHHHHHHHHHHHEEE
VFFLVPTGRGEESDEVSPADYERVLHWFYDMQKESEIQLKATCAPHYFRIMRQRAKKEGI
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCC
EISVKTHGYEAMTKGCLGGTGFCFVSSVGEVYPCGYLPVLAGDIRAQPFKDVWENSEVFR
EEEEEECCHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHCCHHHHH
KLRDPEELKGKCGICEYKKVCAGCRARAYAATGDYLEEEPYCIYRPGKK
HHCCHHHHCCCCCCHHHHHHHHHHHHCEEECCCCCCCCCCEEEECCCCC
>Mature Secondary Structure
MIAMTNPPRLIAWELTAGCNLNCVHCRGASTSSVPEGELTTEEAKHFIDDVVELGKPILI
CCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
LSGGEPLTRTDVFEIARYGTDAGLRVVLATNGTLLTPEIVKKLRDAGVQRLSISLDGATA
EECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCEECHHHHHHHHHCCCEEEEEEECCCCC
KTHDEFRGVPGAFERTLAGIEILRKADFPFQINTTISRRNLEEIPKTFELAKELGAVAYH
CCCHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHCCHHHHHHHHHHHHHHHHHEEE
VFFLVPTGRGEESDEVSPADYERVLHWFYDMQKESEIQLKATCAPHYFRIMRQRAKKEGI
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCC
EISVKTHGYEAMTKGCLGGTGFCFVSSVGEVYPCGYLPVLAGDIRAQPFKDVWENSEVFR
EEEEEECCHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHCCHHHHH
KLRDPEELKGKCGICEYKKVCAGCRARAYAATGDYLEEEPYCIYRPGKK
HHCCHHHHCCCCCCHHHHHHHHHHHHCEEECCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]