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The map label for this gene is pylS

Identifier: 21227547

GI number: 21227547

Start: 1727617

End: 1728981

Strand: Reverse

Name: pylS

Synonym: MM_1445

Alternate gene names: 21227547

Gene position: 1728981-1727617 (Counterclockwise)

Preceding gene: 21227549

Following gene: 21227546

Centisome position: 42.21

GC content: 47.25

Gene sequence:

>1365_bases
ATGGATAAAAAACCACTAAACACTCTGATATCTGCAACCGGGCTCTGGATGTCCAGGACCGGAACAATTCATAAAATAAA
ACACCACGAAGTCTCTCGAAGCAAAATCTATATTGAAATGGCATGCGGAGACCACCTTGTTGTAAACAACTCCAGGAGCA
GCAGGACTGCAAGAGCGCTCAGGCACCACAAATACAGGAAGACCTGCAAACGCTGCAGGGTTTCGGATGAGGATCTCAAT
AAGTTCCTCACAAAGGCAAACGAAGACCAGACAAGCGTAAAAGTCAAGGTCGTTTCTGCCCCTACCAGAACGAAAAAGGC
AATGCCAAAATCCGTTGCGAGAGCCCCGAAACCTCTTGAGAATACAGAAGCGGCACAGGCTCAACCTTCTGGATCTAAAT
TTTCACCTGCGATACCGGTTTCCACCCAAGAGTCAGTTTCTGTCCCGGCATCTGTTTCAACATCAATATCAAGCATTTCT
ACAGGAGCAACTGCATCCGCACTGGTAAAAGGGAATACGAACCCCATTACATCCATGTCTGCCCCTGTTCAGGCAAGTGC
CCCCGCACTTACGAAGAGCCAGACTGACAGGCTTGAAGTCCTGTTAAACCCAAAAGATGAGATTTCCCTGAATTCCGGCA
AGCCTTTCAGGGAGCTTGAGTCCGAATTGCTCTCTCGCAGAAAAAAAGACCTGCAGCAGATCTACGCGGAAGAAAGGGAG
AATTATCTGGGGAAACTCGAGCGTGAAATTACCAGGTTCTTTGTGGACAGGGGTTTTCTGGAAATAAAATCCCCGATCCT
GATCCCTCTTGAGTATATCGAAAGGATGGGCATTGATAATGATACCGAACTTTCAAAACAGATCTTCAGGGTTGACAAGA
ACTTCTGCCTGAGACCCATGCTTGCTCCAAACCTTTACAACTACCTGCGCAAGCTTGACAGGGCCCTGCCTGATCCAATA
AAAATTTTTGAAATAGGCCCATGCTACAGAAAAGAGTCCGACGGCAAAGAACACCTCGAAGAGTTTACCATGCTGAACTT
CTGCCAGATGGGATCGGGATGCACACGGGAAAATCTTGAAAGCATAATTACGGACTTCCTGAACCACCTGGGAATTGATT
TCAAGATCGTAGGCGATTCCTGCATGGTCTATGGGGATACCCTTGATGTAATGCACGGAGACCTGGAACTTTCCTCTGCA
GTAGTCGGACCCATACCGCTTGACCGGGAATGGGGTATTGATAAACCCTGGATAGGGGCAGGTTTCGGGCTCGAACGCCT
TCTAAAGGTTAAACACGACTTTAAAAATATCAAGAGAGCTGCAAGGTCCGAGTCTTACTATAACGGGATTTCTACCAACC
TGTAA

Upstream 100 bases:

>100_bases
TGTCAGCAATTGCTGGCTCCACGCTTATGTTCTGTAAGCCGGGGTTCTGAATACTGTTCTTGGAAATTATCCGGGTTTTA
GTTCACCGAGGGGTAACACT

Downstream 100 bases:

>100_bases
TAATCCTGGACCATGTAAGACACATCTAAAGATTCTCTGAAAAGCTGGAAGTATCAGAAGAAAAGTACCAAAACGGCGAA
AATGGCGATAAATGATGATA

Product: pyrolysyl-tRNA synthetase

Products: NA

Alternate protein names: Pyrrolysine--tRNA ligase; PylRS

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MDKKPLNTLISATGLWMSRTGTIHKIKHHEVSRSKIYIEMACGDHLVVNNSRSSRTARALRHHKYRKTCKRCRVSDEDLN
KFLTKANEDQTSVKVKVVSAPTRTKKAMPKSVARAPKPLENTEAAQAQPSGSKFSPAIPVSTQESVSVPASVSTSISSIS
TGATASALVKGNTNPITSMSAPVQASAPALTKSQTDRLEVLLNPKDEISLNSGKPFRELESELLSRRKKDLQQIYAEERE
NYLGKLEREITRFFVDRGFLEIKSPILIPLEYIERMGIDNDTELSKQIFRVDKNFCLRPMLAPNLYNYLRKLDRALPDPI
KIFEIGPCYRKESDGKEHLEEFTMLNFCQMGSGCTRENLESIITDFLNHLGIDFKIVGDSCMVYGDTLDVMHGDLELSSA
VVGPIPLDREWGIDKPWIGAGFGLERLLKVKHDFKNIKRAARSESYYNGISTNL

Sequences:

>Translated_454_residues
MDKKPLNTLISATGLWMSRTGTIHKIKHHEVSRSKIYIEMACGDHLVVNNSRSSRTARALRHHKYRKTCKRCRVSDEDLN
KFLTKANEDQTSVKVKVVSAPTRTKKAMPKSVARAPKPLENTEAAQAQPSGSKFSPAIPVSTQESVSVPASVSTSISSIS
TGATASALVKGNTNPITSMSAPVQASAPALTKSQTDRLEVLLNPKDEISLNSGKPFRELESELLSRRKKDLQQIYAEERE
NYLGKLEREITRFFVDRGFLEIKSPILIPLEYIERMGIDNDTELSKQIFRVDKNFCLRPMLAPNLYNYLRKLDRALPDPI
KIFEIGPCYRKESDGKEHLEEFTMLNFCQMGSGCTRENLESIITDFLNHLGIDFKIVGDSCMVYGDTLDVMHGDLELSSA
VVGPIPLDREWGIDKPWIGAGFGLERLLKVKHDFKNIKRAARSESYYNGISTNL
>Mature_454_residues
MDKKPLNTLISATGLWMSRTGTIHKIKHHEVSRSKIYIEMACGDHLVVNNSRSSRTARALRHHKYRKTCKRCRVSDEDLN
KFLTKANEDQTSVKVKVVSAPTRTKKAMPKSVARAPKPLENTEAAQAQPSGSKFSPAIPVSTQESVSVPASVSTSISSIS
TGATASALVKGNTNPITSMSAPVQASAPALTKSQTDRLEVLLNPKDEISLNSGKPFRELESELLSRRKKDLQQIYAEERE
NYLGKLEREITRFFVDRGFLEIKSPILIPLEYIERMGIDNDTELSKQIFRVDKNFCLRPMLAPNLYNYLRKLDRALPDPI
KIFEIGPCYRKESDGKEHLEEFTMLNFCQMGSGCTRENLESIITDFLNHLGIDFKIVGDSCMVYGDTLDVMHGDLELSSA
VVGPIPLDREWGIDKPWIGAGFGLERLLKVKHDFKNIKRAARSESYYNGISTNL

Specific function: Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYLS_METMA (Q8PWY1)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633469.1
- PDB:   2E3C
- PDB:   2Q7E
- PDB:   2Q7G
- PDB:   2Q7H
- PDB:   2ZCD
- PDB:   2ZCE
- PDB:   2ZIM
- PDB:   2ZIN
- PDB:   2ZIO
- PDBsum:   2E3C
- PDBsum:   2Q7E
- PDBsum:   2Q7G
- PDBsum:   2Q7H
- PDBsum:   2ZCD
- PDBsum:   2ZCE
- PDBsum:   2ZIM
- PDBsum:   2ZIN
- PDBsum:   2ZIO
- ProteinModelPortal:   Q8PWY1
- SMR:   Q8PWY1
- GeneID:   1479787
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1445
- NMPDR:   fig|192952.1.peg.1445
- HOGENOM:   HBG459849
- OMA:   SGCTREN
- ProtClustDB:   PRK09537
- BioCyc:   MMAZ192952:MM1445-MONOMER
- BRENDA:   6.1.1.26
- GO:   GO:0005737
- HAMAP:   MF_01573
- InterPro:   IPR002314
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR012739
- PANTHER:   PTHR22594
- TIGRFAMs:   TIGR02367

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: =6.1.1.26

Molecular weight: Translated: 50922; Mature: 50922

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKPLNTLISATGLWMSRTGTIHKIKHHEVSRSKIYIEMACGDHLVVNNSRSSRTARAL
CCCCHHHHHHHHHCCCEECCCCEEEHHHCCCCCCEEEEEEECCCEEEEECCCCHHHHHHH
RHHKYRKTCKRCRVSDEDLNKFLTKANEDQTSVKVKVVSAPTRTKKAMPKSVARAPKPLE
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHCHHHHHHCCCCCC
NTEAAQAQPSGSKFSPAIPVSTQESVSVPASVSTSISSISTGATASALVKGNTNPITSMS
CCCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHEEEECCCCCCCCCC
APVQASAPALTKSQTDRLEVLLNPKDEISLNSGKPFRELESELLSRRKKDLQQIYAEERE
CCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NYLGKLEREITRFFVDRGFLEIKSPILIPLEYIERMGIDNDTELSKQIFRVDKNFCLRPM
HHHHHHHHHHHHHHHHCCHHHHCCCEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
LAPNLYNYLRKLDRALPDPIKIFEIGPCYRKESDGKEHLEEFTMLNFCQMGSGCTRENLE
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
SIITDFLNHLGIDFKIVGDSCMVYGDTLDVMHGDLELSSAVVGPIPLDREWGIDKPWIGA
HHHHHHHHHCCCEEEEECCCEEEECCHHHHHCCCHHHCCEEECCCCCCCCCCCCCCCCCC
GFGLERLLKVKHDFKNIKRAARSESYYNGISTNL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDKKPLNTLISATGLWMSRTGTIHKIKHHEVSRSKIYIEMACGDHLVVNNSRSSRTARAL
CCCCHHHHHHHHHCCCEECCCCEEEHHHCCCCCCEEEEEEECCCEEEEECCCCHHHHHHH
RHHKYRKTCKRCRVSDEDLNKFLTKANEDQTSVKVKVVSAPTRTKKAMPKSVARAPKPLE
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHCHHHHHHCCCCCC
NTEAAQAQPSGSKFSPAIPVSTQESVSVPASVSTSISSISTGATASALVKGNTNPITSMS
CCCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHEEEECCCCCCCCCC
APVQASAPALTKSQTDRLEVLLNPKDEISLNSGKPFRELESELLSRRKKDLQQIYAEERE
CCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NYLGKLEREITRFFVDRGFLEIKSPILIPLEYIERMGIDNDTELSKQIFRVDKNFCLRPM
HHHHHHHHHHHHHHHHCCHHHHCCCEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
LAPNLYNYLRKLDRALPDPIKIFEIGPCYRKESDGKEHLEEFTMLNFCQMGSGCTRENLE
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
SIITDFLNHLGIDFKIVGDSCMVYGDTLDVMHGDLELSSAVVGPIPLDREWGIDKPWIGA
HHHHHHHHHCCCEEEEECCCEEEECCHHHHHCCCHHHCCEEECCCCCCCCCCCCCCCCCC
GFGLERLLKVKHDFKNIKRAARSESYYNGISTNL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824