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The map label for this gene is thiH [C]

Identifier: 21227546

GI number: 21227546

Start: 1726334

End: 1727383

Strand: Reverse

Name: thiH [C]

Synonym: MM_1444

Alternate gene names: 21227546

Gene position: 1727383-1726334 (Counterclockwise)

Preceding gene: 21227547

Following gene: 21227545

Centisome position: 42.17

GC content: 45.33

Gene sequence:

>1050_bases
TTGATCCAGAAAATGGCAACCGAAGAACTTGACAGGTTCGGGGAGAAAATTATTGAAGGTTTTAAATTGTCTGATGATGA
CCTCAGGGCTCTTCTTTCTCTTGAATTCGAAGAAGAGCTGGAAAAGCTTTACTATGTAGCTAGAAAGGTCAGAAACTATT
ATTTCGGCAACAGGGTGTTTCTTAACTGTTTTATTTATTTCTCAACTTATTGTAAAAACCAGTGCTCTTTTTGCTACTAT
AACTGTAAAAACGAAATTAACCGCTACCGCCTGACCGGTGAAGAGGTTAAAGAGATGTGCAAAGCCCTGAAAGGTGCAGG
CTTTCACATGATCGACCTGACAATGGGAGAGGATCCCTATTACTATGATGACCCTGACCGCTTCGTTGAACTTGTCAGGA
CAGTAAAAGAAGAACTCGGGCTTCCAATAATGATTTCTCCGGGAGTTATGGATGACAGCACCCTCCTGAAAGCCAGGGAA
GAAGGAGCAAATTTCTTTGCCCTTTATCAGGAGACTTATGACCGCGAACTTTATGGAAAGCTAAGGGTAGGTCAGTCCTT
CGAAGGAAGGTTTAATGCCCGCAGGTTTGCAAAAGAACAGGGGTACTGTATAGAAGACGGCATTCTTACCGGCGTAGGAA
ATGATATCGAATCAACTCTTATATCCCTGAAGGGGATGAAAGCAAACAATCCTGATATGGTAAGGGTAATGACTTTTCTG
CCTCAGGAAGGAACTCCGCTTGAAGGTTTCAGCGATAGTTCAAAGCTTTCGGAGCTGAAAATCATAGCGATTCTCAGGCT
CATGTTTCCTGAATGCCTGATACCGGCTTCTCTTGACCTTGAAGGCATAGACGGCATGGTGCACCGTTTAAATGCCGGAG
CAAATATTGTAACCTCCATCCTCCCAGATTCACGCCTGGAAGGGGTTGCCAATTACGACCGCGGCATGGAAGAGAGGGAC
AGGGACGTTACAAGCGTTGTCAAAAGGCTGAAGGTTATGGGAATGGAACCTGCGCCGCAGGCTGAGTTTGAGAGAGTCCT
GGGGTGCTAG

Upstream 100 bases:

>100_bases
ACTTAAAATGGCAAAAAGATCCGGATGGGGCTGACGCGAAAGGAATTTGAAAGTAAGGTTGTAAGGATAAAAAATTTTAA
ATCTATGAGAGGGTGAAAGT

Downstream 100 bases:

>100_bases
CCTGAAAACAATATGCCTTATAGGCGGGAAGCTGCAGGGCTTCGAGGCTGCATACCTATCTAAGAAAGCCGGAATGAAAG
TGCTTGTAATAGACAAAAAC

Product: biotin synthetase

Products: biotin

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MIQKMATEELDRFGEKIIEGFKLSDDDLRALLSLEFEEELEKLYYVARKVRNYYFGNRVFLNCFIYFSTYCKNQCSFCYY
NCKNEINRYRLTGEEVKEMCKALKGAGFHMIDLTMGEDPYYYDDPDRFVELVRTVKEELGLPIMISPGVMDDSTLLKARE
EGANFFALYQETYDRELYGKLRVGQSFEGRFNARRFAKEQGYCIEDGILTGVGNDIESTLISLKGMKANNPDMVRVMTFL
PQEGTPLEGFSDSSKLSELKIIAILRLMFPECLIPASLDLEGIDGMVHRLNAGANIVTSILPDSRLEGVANYDRGMEERD
RDVTSVVKRLKVMGMEPAPQAEFERVLGC

Sequences:

>Translated_349_residues
MIQKMATEELDRFGEKIIEGFKLSDDDLRALLSLEFEEELEKLYYVARKVRNYYFGNRVFLNCFIYFSTYCKNQCSFCYY
NCKNEINRYRLTGEEVKEMCKALKGAGFHMIDLTMGEDPYYYDDPDRFVELVRTVKEELGLPIMISPGVMDDSTLLKARE
EGANFFALYQETYDRELYGKLRVGQSFEGRFNARRFAKEQGYCIEDGILTGVGNDIESTLISLKGMKANNPDMVRVMTFL
PQEGTPLEGFSDSSKLSELKIIAILRLMFPECLIPASLDLEGIDGMVHRLNAGANIVTSILPDSRLEGVANYDRGMEERD
RDVTSVVKRLKVMGMEPAPQAEFERVLGC
>Mature_349_residues
MIQKMATEELDRFGEKIIEGFKLSDDDLRALLSLEFEEELEKLYYVARKVRNYYFGNRVFLNCFIYFSTYCKNQCSFCYY
NCKNEINRYRLTGEEVKEMCKALKGAGFHMIDLTMGEDPYYYDDPDRFVELVRTVKEELGLPIMISPGVMDDSTLLKARE
EGANFFALYQETYDRELYGKLRVGQSFEGRFNARRFAKEQGYCIEDGILTGVGNDIESTLISLKGMKANNPDMVRVMTFL
PQEGTPLEGFSDSSKLSELKIIAILRLMFPECLIPASLDLEGIDGMVHRLNAGANIVTSILPDSRLEGVANYDRGMEERD
RDVTSVVKRLKVMGMEPAPQAEFERVLGC

Specific function: Required For The Synthesis Of The Thiazole Moiety Of Thiamine. [C]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790423, Length=167, Percent_Identity=26.3473053892216, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: 2.8.1.6

Molecular weight: Translated: 39959; Mature: 39959

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKMATEELDRFGEKIIEGFKLSDDDLRALLSLEFEEELEKLYYVARKVRNYYFGNRVF
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LNCFIYFSTYCKNQCSFCYYNCKNEINRYRLTGEEVKEMCKALKGAGFHMIDLTMGEDPY
HHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCC
YYDDPDRFVELVRTVKEELGLPIMISPGVMDDSTLLKAREEGANFFALYQETYDRELYGK
EECCHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHH
LRVGQSFEGRFNARRFAKEQGYCIEDGILTGVGNDIESTLISLKGMKANNPDMVRVMTFL
HHCCCCCCCCHHHHHHHHHCCCEEECCCEECCCCHHHHHHHHHCCCCCCCCCHHHHEECC
PQEGTPLEGFSDSSKLSELKIIAILRLMFPECLIPASLDLEGIDGMVHRLNAGANIVTSI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHH
LPDSRLEGVANYDRGMEERDRDVTSVVKRLKVMGMEPAPQAEFERVLGC
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MIQKMATEELDRFGEKIIEGFKLSDDDLRALLSLEFEEELEKLYYVARKVRNYYFGNRVF
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LNCFIYFSTYCKNQCSFCYYNCKNEINRYRLTGEEVKEMCKALKGAGFHMIDLTMGEDPY
HHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCC
YYDDPDRFVELVRTVKEELGLPIMISPGVMDDSTLLKAREEGANFFALYQETYDRELYGK
EECCHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHH
LRVGQSFEGRFNARRFAKEQGYCIEDGILTGVGNDIESTLISLKGMKANNPDMVRVMTFL
HHCCCCCCCCHHHHHHHHHCCCEEECCCEECCCCHHHHHHHHHCCCCCCCCCHHHHEECC
PQEGTPLEGFSDSSKLSELKIIAILRLMFPECLIPASLDLEGIDGMVHRLNAGANIVTSI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHH
LPDSRLEGVANYDRGMEERDRDVTSVVKRLKVMGMEPAPQAEFERVLGC
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]