Click here to switch to the map view.

The map label for this gene is priB

Identifier: 21227498

GI number: 21227498

Start: 1679809

End: 1680921

Strand: Reverse

Name: priB

Synonym: MM_1396

Alternate gene names: 21227498

Gene position: 1680921-1679809 (Counterclockwise)

Preceding gene: 21227499

Following gene: 21227495

Centisome position: 41.03

GC content: 44.29

Gene sequence:

>1113_bases
ATGCAGGCAGAAAAACTTGCATATTACCCATTCACTTCAGAAGCGTCTGCCTACGTTGGAAACCTGGGAATTTCCTTAGA
AAGCCTGCTCAATTCGCGGGCTTACAGGGCTGCAAGAGCCCGTGGAATAGAAAGGGTAAAAGAAGCTCTTGAAGGAGAAA
TTAAAAAATCTCCTGTTTCGGGGGAAGCCCAGGTACTTTCAGAACTCCTTTCCTATCCATTTGCCAGGATGCTGGTCGCC
TGCGTGGATGATCAACTCTTTACCCGACGTTATGCTCTGGCAGAAGCAAAGGCTGCTTATACTTTTTTGAGAAACGAAAA
TCCGGATTTTCTGCTGGAATTCGGAGATGATTTTGGAATTTCAGCAGATTCTCAGGATTCTTATTTCAGCATGCATTTTA
CGGACTATATCCGCTTTTCAAATTCCCTTAAAGACCCTTCATGGAAACTGACAAACCGCCAGTTGAGAGCAGGCAAAATA
AAAATAACAAAAGAAGAGTTTTCAAGGCTTCTTGAAGAAGCGGTCAGGGAAAGGATTGAACAATCCTTCCCTGTCCCGGA
AATCCCGCCCGAGGTATCCAGTTTCTGTTCACCTTACGCTGCCGAGATAAAGGACAAGTTCGAAGTCCAGAAAAAGAAAT
TCGGATCTACGGATTTCGGAGCTGTCAAACCGGAACTATTCCCCCCCTGCATTGCCTATGCGCTTGCTAACGTTCAGGGA
GGAGTGAATCTTGCTCACTCGATGCGTTTTGCAATGACATCCTTCCTTCTCAATGTGGGAATGTCAGTAGATGAAATCCT
GAACCTTTTCAATATCTCACCGGATTTTAATGCAGAAAAAACCCTCTACCAGATAGAGCATATCGCAGGTGCTACAGGAA
ATACTTACAAACCACCTGCCTGCGACACCATGAGGACGTATGGGAACTGCGTAGGAAAAGACAGGCTCTGCGAGAAAATA
AGCCATCCTCTTGGATATTACGAGAAAAAAGTATTCATAAAAAATAAAGAAGGAGAAGAGGCACAGGGAAAAGAACAGGG
AAAGGAGAAGGATGATGGGAAGGAAAAGGAAAACGGGAAGGAAAGTGAAGTGAAAAAGAAGAAAGAGAAGTGA

Upstream 100 bases:

>100_bases
GGAAGGGATTTCCCCATCCTGATTGATTTCGAGATTGCAAACGGTGCGGGCAGAATCTCTTACCTGCTGGCTCCGAGAAT
CGAATCGGACTAAGGATGAC

Downstream 100 bases:

>100_bases
AAAAGAAGAAAGAGAAGTGAAAAAAGAAGTAACAGCAAATGAATAATGTAAAACTAAATTGAAAGTCGGGACTGCCAGCC
ATCAATTGTTCATAAAAACT

Product: DNA primase large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MQAEKLAYYPFTSEASAYVGNLGISLESLLNSRAYRAARARGIERVKEALEGEIKKSPVSGEAQVLSELLSYPFARMLVA
CVDDQLFTRRYALAEAKAAYTFLRNENPDFLLEFGDDFGISADSQDSYFSMHFTDYIRFSNSLKDPSWKLTNRQLRAGKI
KITKEEFSRLLEEAVRERIEQSFPVPEIPPEVSSFCSPYAAEIKDKFEVQKKKFGSTDFGAVKPELFPPCIAYALANVQG
GVNLAHSMRFAMTSFLLNVGMSVDEILNLFNISPDFNAEKTLYQIEHIAGATGNTYKPPACDTMRTYGNCVGKDRLCEKI
SHPLGYYEKKVFIKNKEGEEAQGKEQGKEKDDGKEKENGKESEVKKKKEK

Sequences:

>Translated_370_residues
MQAEKLAYYPFTSEASAYVGNLGISLESLLNSRAYRAARARGIERVKEALEGEIKKSPVSGEAQVLSELLSYPFARMLVA
CVDDQLFTRRYALAEAKAAYTFLRNENPDFLLEFGDDFGISADSQDSYFSMHFTDYIRFSNSLKDPSWKLTNRQLRAGKI
KITKEEFSRLLEEAVRERIEQSFPVPEIPPEVSSFCSPYAAEIKDKFEVQKKKFGSTDFGAVKPELFPPCIAYALANVQG
GVNLAHSMRFAMTSFLLNVGMSVDEILNLFNISPDFNAEKTLYQIEHIAGATGNTYKPPACDTMRTYGNCVGKDRLCEKI
SHPLGYYEKKVFIKNKEGEEAQGKEQGKEKDDGKEKENGKESEVKKKKEK
>Mature_370_residues
MQAEKLAYYPFTSEASAYVGNLGISLESLLNSRAYRAARARGIERVKEALEGEIKKSPVSGEAQVLSELLSYPFARMLVA
CVDDQLFTRRYALAEAKAAYTFLRNENPDFLLEFGDDFGISADSQDSYFSMHFTDYIRFSNSLKDPSWKLTNRQLRAGKI
KITKEEFSRLLEEAVRERIEQSFPVPEIPPEVSSFCSPYAAEIKDKFEVQKKKFGSTDFGAVKPELFPPCIAYALANVQG
GVNLAHSMRFAMTSFLLNVGMSVDEILNLFNISPDFNAEKTLYQIEHIAGATGNTYKPPACDTMRTYGNCVGKDRLCEKI
SHPLGYYEKKVFIKNKEGEEAQGKEQGKEKDDGKEKENGKESEVKKKKEK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG2219

COG function: function code L; Eukaryotic-type DNA primase, large subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic-type primase large subunit family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIL_METMA (Q8PX26)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_633420.1
- ProteinModelPortal:   Q8PX26
- SMR:   Q8PX26
- GeneID:   1479738
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_1396
- NMPDR:   fig|192952.1.peg.1396
- HOGENOM:   HBG734019
- OMA:   PSCATMQ
- ProtClustDB:   PRK02249
- BioCyc:   MMAZ192952:MM1396-MONOMER
- HAMAP:   MF_00701
- InterPro:   IPR007238

Pfam domain/function: PF04104 DNA_primase_lrg

EC number: NA

Molecular weight: Translated: 41699; Mature: 41699

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAEKLAYYPFTSEASAYVGNLGISLESLLNSRAYRAARARGIERVKEALEGEIKKSPVS
CCCCCEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCC
GEAQVLSELLSYPFARMLVACVDDQLFTRRYALAEAKAAYTFLRNENPDFLLEFGDDFGI
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
SADSQDSYFSMHFTDYIRFSNSLKDPSWKLTNRQLRAGKIKITKEEFSRLLEEAVRERIE
CCCCCCCEEEEHHHHHHHHHCCCCCCCCHHCCCHHCCCEEEECHHHHHHHHHHHHHHHHH
QSFPVPEIPPEVSSFCSPYAAEIKDKFEVQKKKFGSTDFGAVKPELFPPCIAYALANVQG
HCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
GVNLAHSMRFAMTSFLLNVGMSVDEILNLFNISPDFNAEKTLYQIEHIAGATGNTYKPPA
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
CDTMRTYGNCVGKDRLCEKISHPLGYYEKKVFIKNKEGEEAQGKEQGKEKDDGKEKENGK
HHHHHHHHHHCCHHHHHHHHHCCCCHHHCEEEEECCCCCCCCCHHCCCCCCCCCHHCCCC
ESEVKKKKEK
HHHHHHHCCC
>Mature Secondary Structure
MQAEKLAYYPFTSEASAYVGNLGISLESLLNSRAYRAARARGIERVKEALEGEIKKSPVS
CCCCCEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCC
GEAQVLSELLSYPFARMLVACVDDQLFTRRYALAEAKAAYTFLRNENPDFLLEFGDDFGI
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
SADSQDSYFSMHFTDYIRFSNSLKDPSWKLTNRQLRAGKIKITKEEFSRLLEEAVRERIE
CCCCCCCEEEEHHHHHHHHHCCCCCCCCHHCCCHHCCCEEEECHHHHHHHHHHHHHHHHH
QSFPVPEIPPEVSSFCSPYAAEIKDKFEVQKKKFGSTDFGAVKPELFPPCIAYALANVQG
HCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
GVNLAHSMRFAMTSFLLNVGMSVDEILNLFNISPDFNAEKTLYQIEHIAGATGNTYKPPA
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
CDTMRTYGNCVGKDRLCEKISHPLGYYEKKVFIKNKEGEEAQGKEQGKEKDDGKEKENGK
HHHHHHHHHHCCHHHHHHHHHCCCCHHHCEEEEECCCCCCCCCHHCCCCCCCCCHHCCCC
ESEVKKKKEK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824