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The map label for this gene is pcn [H]

Identifier: 21227499

GI number: 21227499

Start: 1680929

End: 1681666

Strand: Reverse

Name: pcn [H]

Synonym: MM_1397

Alternate gene names: 21227499

Gene position: 1681666-1680929 (Counterclockwise)

Preceding gene: 21227500

Following gene: 21227498

Centisome position: 41.05

GC content: 46.75

Gene sequence:

>738_bases
ATGTTCAAGGCAGCAATTAATGCAGAGCTTCTGAAAGACGCAATTGCCTCACTGGCTGTAATTGTAGACGAGGTCAGATT
CAAAATTAAACCCGAAGGTATTTCGGTAAAGGCGGTTGATCCCGCCAACGTTGCAATGGGGATTTTTGAGCTTGGGTCAT
CAGCTTTTGAGGAATATAGTGCTGATGAGTGTGAAATAGGGATCGATCTGAATAAGATTACCGACCTCCTGGGAATTGCG
GACAGGAACGATACAGTACGTATGGAGCTTGATGAAGGTAGCAATAAGCTCCTGATTGATGTTGGAGGATTATCATATAC
TCTTTCTCTTCTGGACCCTTCAACAATCCGTGCAGAGCCCAGAGTTCCTCAGCTCGAACTTCCTGCCAAAGTTGTCCTGA
ACGGTGCAGACCTCAGGCGTGCCGTAAAAGCCGCAGAAAAAATAAGCGACCACATGCTTATGGGAGTCTCTGGCGATACT
TTTTATATGGAAGCAAAGGGCGATACAGATCAGGTCCGCCTGGAAATGGGTAGAGACCAGTTAATCGACCTTAAAGCAGG
TGAAGCCTGTTCCCTCTTCTCCCTGGACTACCTTACAGATATAGTAAAACCCACAAACAAGGTAAATGAGGTCACTCTCT
CCCTTGGAAGGGATTTCCCCATCCTGATTGATTTCGAGATTGCAAACGGTGCGGGCAGAATCTCTTACCTGCTGGCTCCG
AGAATCGAATCGGACTAA

Upstream 100 bases:

>100_bases
TTACCTTCAAGGGATAGAAAAATCCCAAATTGTAAGGAAATTTATATATCCAGTGATAGATAAGGAGTTTAAGGCTTAAT
TTATTTGATGGAGAAGAATC

Downstream 100 bases:

>100_bases
GGATGACATGCAGGCAGAAAAACTTGCATATTACCCATTCACTTCAGAAGCGTCTGCCTACGTTGGAAACCTGGGAATTT
CCTTAGAAAGCCTGCTCAAT

Product: DNA polymerase sliding clamp

Products: NA

Alternate protein names: Proliferating cell nuclear antigen homolog; PCNA [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MFKAAINAELLKDAIASLAVIVDEVRFKIKPEGISVKAVDPANVAMGIFELGSSAFEEYSADECEIGIDLNKITDLLGIA
DRNDTVRMELDEGSNKLLIDVGGLSYTLSLLDPSTIRAEPRVPQLELPAKVVLNGADLRRAVKAAEKISDHMLMGVSGDT
FYMEAKGDTDQVRLEMGRDQLIDLKAGEACSLFSLDYLTDIVKPTNKVNEVTLSLGRDFPILIDFEIANGAGRISYLLAP
RIESD

Sequences:

>Translated_245_residues
MFKAAINAELLKDAIASLAVIVDEVRFKIKPEGISVKAVDPANVAMGIFELGSSAFEEYSADECEIGIDLNKITDLLGIA
DRNDTVRMELDEGSNKLLIDVGGLSYTLSLLDPSTIRAEPRVPQLELPAKVVLNGADLRRAVKAAEKISDHMLMGVSGDT
FYMEAKGDTDQVRLEMGRDQLIDLKAGEACSLFSLDYLTDIVKPTNKVNEVTLSLGRDFPILIDFEIANGAGRISYLLAP
RIESD
>Mature_245_residues
MFKAAINAELLKDAIASLAVIVDEVRFKIKPEGISVKAVDPANVAMGIFELGSSAFEEYSADECEIGIDLNKITDLLGIA
DRNDTVRMELDEGSNKLLIDVGGLSYTLSLLDPSTIRAEPRVPQLELPAKVVLNGADLRRAVKAAEKISDHMLMGVSGDT
FYMEAKGDTDQVRLEMGRDQLIDLKAGEACSLFSLDYLTDIVKPTNKVNEVTLSLGRDFPILIDFEIANGAGRISYLLAP
RIESD

Specific function: Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication [H]

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PCNA family [H]

Homologues:

Organism=Homo sapiens, GI33239451, Length=260, Percent_Identity=22.6923076923077, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4505641, Length=260, Percent_Identity=22.6923076923077, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319564, Length=260, Percent_Identity=25, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136790, Length=261, Percent_Identity=21.455938697318, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45552759, Length=261, Percent_Identity=21.455938697318, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000730
- InterPro:   IPR022649
- InterPro:   IPR022659
- InterPro:   IPR022648 [H]

Pfam domain/function: PF02747 PCNA_C; PF00705 PCNA_N [H]

EC number: NA

Molecular weight: Translated: 26650; Mature: 26650

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS01251 PCNA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKAAINAELLKDAIASLAVIVDEVRFKIKPEGISVKAVDPANVAMGIFELGSSAFEEYS
CCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCHHHHHHHHHHCHHHHHHCC
ADECEIGIDLNKITDLLGIADRNDTVRMELDEGSNKLLIDVGGLSYTLSLLDPSTIRAEP
CCCEEECCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCEEEEEECCCCCCCCCC
RVPQLELPAKVVLNGADLRRAVKAAEKISDHMLMGVSGDTFYMEAKGDTDQVRLEMGRDQ
CCCCCCCCCEEEECCHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCEEEEECCCCE
LIDLKAGEACSLFSLDYLTDIVKPTNKVNEVTLSLGRDFPILIDFEIANGAGRISYLLAP
EEEECCCCCEEEEEHHHHHHHHCCCCCHHEEEEECCCCCCEEEEEEECCCCCEEEEEECC
RIESD
CCCCC
>Mature Secondary Structure
MFKAAINAELLKDAIASLAVIVDEVRFKIKPEGISVKAVDPANVAMGIFELGSSAFEEYS
CCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCHHHHHHHHHHCHHHHHHCC
ADECEIGIDLNKITDLLGIADRNDTVRMELDEGSNKLLIDVGGLSYTLSLLDPSTIRAEP
CCCEEECCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCEEEEEECCCCCCCCCC
RVPQLELPAKVVLNGADLRRAVKAAEKISDHMLMGVSGDTFYMEAKGDTDQVRLEMGRDQ
CCCCCCCCCEEEECCHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCEEEEECCCCE
LIDLKAGEACSLFSLDYLTDIVKPTNKVNEVTLSLGRDFPILIDFEIANGAGRISYLLAP
EEEECCCCCEEEEEHHHHHHHHCCCCCHHEEEEECCCCCCEEEEEEECCCCCEEEEEECC
RIESD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]